DD277828A1 - MEANS FOR INCREASING THE STRESSTOLERANCE OF CULTURAL PLANTS - Google Patents

Abstract

The invention relates to agents which are used to increase the stress resistance of crops, in particular for the simultaneous increase of the salt and drought tolerance of cereals. As active ingredient, these agents contain a mixture of monoethanolamine of the formula IHOCH2CH2NH2 (formula I) and at least one brassinolide of the general formula II in which R is a straight-chain or branched alkyl radical which has up to 8 C atoms, bears a vicinal glycol grouping and can have a double bond; Monoethanolamine may be present in this mixture as the free base or as the salt of the mineral acid. Formula II

Description

in which R is a straight-chain or branched alkyl radical which has up to 8 C atoms, carries a vicinal glycol moiety and may have one C-C double bond, monoethanolamine being present as the free base or as the salt of a mineral acid. 2. Composition according to claim 1, characterized in that they contain, in addition to customary excipients and carriers as the active ingredient, a mixture of monoethanolamine of the formula I and (22S, 23S) -Hcmobrassinolid.

Field of application of the invention

The invention relates to a Mitlei to increase the stress tolerance of crops. It can be used in particular for improving the tolerance of crops to abiotic stressors in agricultural crop production.

Characteristic of the known state of the art

It is well known that yield depression is primarily caused by physiological dryness. In addition, about 1 billion hectares of agricultural land in the world have yield-reducing salinity in the soil substrate. Even in humid climates, the salt concentration in the soil water during temporary drought, especially at high mineral fertilization, increases to phytodynamically unfavorable values. Particularly sensitive to salt are known to be germinating plants and young plants. To mitigate this ertragssenkenden salts effect c Bodenbchandlungen be made with gypsum, for example, but which are associated with an enormous transport and energy costs. It is also known to solve the salt problem by breeding salt-tolerant plants. Significant successes can not yet be achieved in this way. It is known that improvements are possible by using phytohormones and synthetic action analogs of the phytohormones (DDPS 218260, DDPS 217408, DDPS 217409 and DDPS 217410). It is known that an increase in the plant salt tolerance can be achieved when growth-regulating quarternary ammonium bases are used (DD-PS 215686, DD-PS 215688). However, the action of the phytohormones and synthetic growth regulators is inadequate under certain conditions. The uncertainty of the Gibber ßllino and cytokinins with Phytohormoncharaktcr is u. a. that these substances increase transpiration at the same time and thereby lower the water status stability of the plants. When applying the growth inhibitor chlorocholine chloride, the threshold of growth-inhibiting influences can be exceeded under salt conditions, so that then l: a yield advantage results from the use of funds. Furthermore, the production of phytohormones is costly and the use of chlorocholine chloride has toxicological problems. Toxicologically unfavorable saline stress-reducing hydroxylamine derivatives (DDPS 216159) are also to be evaluated. Eino salt tolerance improvement, which is coupled with a yield-increasing water balance optimization, is also achieved by low-cost biogenic and synthetic ethanolamines on individual plants and in Feldfeständon (DD-PS 255871, DDPS 151104).

By applying brassinolides, salt tolerance, herbicidal resistance and resistance to crop herbicides can be increased while avoiding toxicological disadvantages (IPPS 57-118b03, JP-PS SO-113567, OEOS 3533633). However, brassinide is expensive, so that a practical use is linked to a higher yielding activity under field conditions, or less, or a dose reduction with the same efficacy.

Object of the invention

The aim of the invention is to wrap around a means for increasing the stress tolerance of crop plants, in particular for increasing Saiz and rock tolerance with improved resistance-physiological activity, which are kos'enpunst ^: g manufacturable and have toxicologically unfavorable properties and are environmentally safe ,

Explanation of the essence of the invention

The invention has for its object to develop means for increasing nigh stress tolerance of crops, in particular for the simultaneous increase of the salt and drought tolerance of cereals, which are toxicologically and environmentally harmless and act in the salzempfia-ilichen germination and young plant periodo strawschwachend. This object is achieved in that means for increasing the stress tolerance of crops in addition to conventional excipients and carriers as the active ingredient, a mixture of monoethanolamine of the formula I.

HO-CHr-CH ₇ -NH ;, (formula I)

and at least one brassinolide of general formula II ₍

(Formula II)

in which R is a straight chain (branched or branched alkyl radical having up to 8 carbon atoms, carries a vicinalo Glycolgruppierung and may have a C-CDoppelbindung containing monoelhanolamine as the free base or as the salt of a mineral acid in this Mixture vorliegon can.

embodiments

Example 1:

Influence of erfindungstjtmäßen means on the Kelmlings growth of spring barley under salt stress investigations on seedling growth are in Orgomental in Petri dish. Service. For this purpose seed of Sommergeiste (cv. Salome) was pre-swollen either in distilled water or in saline solutions for 5 hours. A portion of the water or saline solution was added before swelling the grains with different amounts of the agents of the invention. Following the pre-quenching phase, the kernels were placed on wet filter paper in sealed petri dishes. The seedling growth was determined 48 hours after the kernels had been placed in the Petri dishes (culturing at 23 C in a darkroom). From the germination experiment it is apparent that under the conditions of salt stress, the use of the agent according to the invention significantly stimulates the growth of crows (18%). The growth effect was clearly above the effects of 1 ppm S.S.SBrassinolid... And 60ppm monoethanolamine, which had been used as a standard by regulation. The results further show that the ratio of S, S, S-homobrassinosteroid to monoethanolamine should be 1 (valley). 1).

Example 2:

Influence of the inventive agents on root growth of barley plants bsi physiological dryness Culturing animal barley plants in small vessels (70Og silica sand / GefäiJ) following fertilization asked been performed (details per vessel): 60mg N, 44 mg P, 77mg K, 3InKjCa, 112mg Mg, 5 Found Mikronährslof!, ·. The seed was then sown in aqueous solutions of the agents according to the invention odor aqua dest (Konirollvariante) vorg <-q>) o! ', Yn (swelling time Sh) and then in the filled with sand vessels (15 grains / vessel). During the 2-leaf stage, the barley plants are again treated with aqueous solutions of the agents according to the invention (spray application). After foliar application, the plants cultured initially at 60% of usable water capacity (nWKt iio;> soil) were removed over 7 days

soil moisture 20 ... 25Ό, so that the plants were exposed to a stressor complex (lack of water, increase in salt retention in the soil and additional Blalterwärmung). The relative root dicMe was determined:

_. , ,,, .... root mass

Relative root density = -

Wurzellängb

Root masonry = root logging per vessel Root length = mean root length per vessel

Table 1: Influence of the agents according to the invention on the seedling growth of spring barley (variety Salome) under goringem to moderate salt stress "

Medium ' ¹ concentration a) according to the invention EA: 6ppm (1 x Br: Ippm 10 ⁴ minor) seedling EA-Br mixture Br: 1 ppm Br: 0.1 ppm growth EA: 60 ppm (1 Br: lOppm • 10 ³ mol / l) Br: 1 ppm EA: 6 ppm 118 ' EA: 0,6ppm (1 EA: COppm • 10 ⁶ mol / l) Gr: 1 ppm EA: 0.6 ppm 108 EA: 6ppm (1 10 ⁴ minor) Br: 0.1 ppm - 103 EA: 6ppm (1 x 10 ⁴ minor) Br: 1öppm 111 b) Comparative variants ³ ' (22S.23S) - d) without means and without salt stress 104 Homobrassi- nolid - Monoetha- 110 ethanolamine 105 (EA) 103 c) without funds 102 - Control 106 98 100 (59.0 mg Koimmasse / lOOKörner) 108

1) Salunchalt of Losuno: 0.25% NaCl, 0.1% KCl 2I EA: Monoethanolatnine Br: I22S, 23SI Homobrassinolide 3I Dosage of the comparator preparations acc. Literature or palm origin * u * 0.05 (TukcyTest). Results from 3 reviews

Table 2: Influence of the agents according to the invention on the root growth of summer barley plants (variety Salome) under physiological dryness

Medium" inventively root density a) EABr-Gomisch'60ppm'1 ppm) EA-B 'mixture (600pp: n / 1ppm) 128 ·· EA-Br mixture (6 ppm / 1 ppm) 119 * Compare versions 116 * ti) EA (600 ppm) Rr; i ppm) 107 without funds 112 C) control 100 without Millet (= 0.32) d) without stress 137 '

1) First treatment - swell seeds in preparation solution; / wide treatment ^; Spray application in aqueous solution (2niljo vessel)

Egg: monoethanolamine Br: (22S, 23S) Hornobrcssinoliil • η. 0:05; · <Α · 0.01 (Tukoy-Tcst) By using the agent according to the invention, the dioecinotic root density was significantly increased (28 ° b). The improvement in root density corresponded in trend to the tester without stress. The fatigue attenuator effect of the preparation according to the invention was also similar to that used for the comparator preparations which were used according to the rules for foliar plucking.

Claims (1)

1. A means for increasing the stress tolerance of crops, in particular for the simultaneous increase of the salt and drought tolerance of cereals, characterized in that in addition to conventional auxiliary and Trägerstoifen as active ingredient, a mixture of monoethanolamine of the formula I. HO-CH ₂ -CH ₂ -NH ₂ (Formula I) and at least one brassinolide of general formula II (Formelll)