CY1115053T1 - Σταθερη παραγωγη λεντοϊικων φορεων - Google Patents
Σταθερη παραγωγη λεντοϊικων φορεωνInfo
- Publication number
- CY1115053T1 CY1115053T1 CY20141100321T CY141100321T CY1115053T1 CY 1115053 T1 CY1115053 T1 CY 1115053T1 CY 20141100321 T CY20141100321 T CY 20141100321T CY 141100321 T CY141100321 T CY 141100321T CY 1115053 T1 CY1115053 T1 CY 1115053T1
- Authority
- CY
- Cyprus
- Prior art keywords
- cell lines
- structural
- proteins
- regulatory
- stable
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000004806 packaging method and process Methods 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 2
- 230000001105 regulatory effect Effects 0.000 abstract 2
- 108700010908 HIV-1 proteins Proteins 0.000 abstract 1
- 241000725303 Human immunodeficiency virus Species 0.000 abstract 1
- 101710150344 Protein Rev Proteins 0.000 abstract 1
- 101710172711 Structural protein Proteins 0.000 abstract 1
- 108091006104 gene-regulatory proteins Proteins 0.000 abstract 1
- 102000034356 gene-regulatory proteins Human genes 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 108010089520 pol Gene Products Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000010473 stable expression Effects 0.000 abstract 1
- 241000701447 unidentified baculovirus Species 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/866—Baculoviral vectors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/867—Retroviral vectors
- C12N15/8673—Special methods for packaging systems
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/02—Recovery or purification
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/21—Retroviridae, e.g. equine infectious anemia virus
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/14011—Baculoviridae
- C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
- C12N2710/14141—Use of virus, viral particle or viral elements as a vector
- C12N2710/14144—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16051—Methods of production or purification of viral material
- C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14311—Parvovirus, e.g. minute virus of mice
- C12N2750/14351—Methods of production or purification of viral material
- C12N2750/14352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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- C12N2800/00—Nucleic acids vectors
- C12N2800/40—Systems of functionally co-operating vectors
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- C12N2800/00—Nucleic acids vectors
- C12N2800/50—Vectors for producing vectors
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- C12N2810/00—Vectors comprising a targeting moiety
- C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
- C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
- C12N2810/6045—RNA rev transcr viruses
- C12N2810/6054—Retroviridae
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Control Of Eletrric Generators (AREA)
Abstract
Η παρούσα εφεύρεση παρέχει νέες σταθερές κυτταρικές σειρές συσκευασίας και κυτταρικές σειρές παραγωγούς καθώς επίσης και μεθόδους για τη λήψη τους, και μια νέα μέθοδο για την παραγωγή λεντοϊικών φορέων με τη χρήση αυτών των κυτταρικών σειρών. Οι νέες μέθοδοι και οι κυτταρικές σειρές συσκευασίας της εφεύρεσης παράγονται με τη χρήση ενός βακιλο-AAV υβριδικού συστήματος για σταθερή έκφραση δομικών και ρυθμιστικών λεντοϊικών πρωτεϊνών, με αυτό το σύστημα να περιλαμβάνει μια βακιλοϊική ραχοκοκαλιά που περιέχει μια κασέτα ενσωμάτωσης που πλευροκοπείται από AAV FTR, σε συνδυασμό με μια rep πρωτεΐνη που κωδικοποιεί πλασμίδιο. Αυτό το σύστημα επιτρέπει την επίτευξη μιας σταθερής ενσωμάτωσης των δομικών και ρυθμιστικών HIV-1 πρωτεϊνών gag/pol και rev. Το σύστημα επιτρέπει τη λήψη ενός πρώτου ενδιαμέσου που περιλαμβάνει μόνο τις δομικές και ρυθμιστικές HIV πρωτεΐνες gag/pol και rev, για να χρησιμοποιηθεί ως σημείο εκκίνησης για τη λήψη σταθερών κυτταρικών σειρών συσκευασίας καθώς επίσης και κυτταρικών σειρών παραγωγών.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10175088 | 2010-09-02 | ||
EP11752530.3A EP2480678B1 (en) | 2010-09-02 | 2011-09-01 | Stable production of lentiviral vectors |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1115053T1 true CY1115053T1 (el) | 2016-12-14 |
Family
ID=44534436
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20141100321T CY1115053T1 (el) | 2010-09-02 | 2014-05-06 | Σταθερη παραγωγη λεντοϊικων φορεων |
Country Status (23)
Country | Link |
---|---|
US (3) | US9441245B2 (el) |
EP (1) | EP2480678B1 (el) |
JP (1) | JP5897577B2 (el) |
KR (1) | KR101990068B1 (el) |
CN (1) | CN103228790B (el) |
AU (1) | AU2011298333B2 (el) |
BR (2) | BR112013005139A2 (el) |
CA (1) | CA2809453C (el) |
CY (1) | CY1115053T1 (el) |
DK (2) | DK2480678T3 (el) |
ES (2) | ES2537184T3 (el) |
HK (2) | HK1184822A1 (el) |
HR (1) | HRP20140392T1 (el) |
IL (2) | IL224520A (el) |
NZ (2) | NZ607686A (el) |
PL (1) | PL2480678T3 (el) |
PT (2) | PT2480677E (el) |
RS (1) | RS53344B (el) |
RU (2) | RU2577979C2 (el) |
SI (1) | SI2480678T1 (el) |
SM (1) | SMT201400058B (el) |
WO (1) | WO2012028681A1 (el) |
ZA (1) | ZA201300998B (el) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103228790B (zh) * | 2010-09-02 | 2015-08-19 | 莫尔梅德股份有限公司 | 慢病毒载体的稳定生产 |
CA2809447C (en) | 2010-09-02 | 2019-12-03 | Molmed S.P.A. | Semi-stable production of lentiviral vectors |
CA2848939A1 (en) | 2011-10-05 | 2013-04-11 | Molmed Spa | Viral vectors purification system |
PT2956477T (pt) | 2013-02-15 | 2021-02-05 | Bioverativ Therapeutics Inc | Gene do fator viii otimizado |
EP3302556A4 (en) * | 2015-05-29 | 2018-12-05 | Clark Atlanta University | Human cell lines mutant for zic2 |
US10450574B2 (en) | 2015-11-24 | 2019-10-22 | Glaxosmithkline Intellectual Property Development Limited | Transient transfection method for retroviral production |
KR102091957B1 (ko) * | 2015-11-24 | 2020-03-20 | 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 | 레트로바이러스 생산을 위한 안정한 세포주 |
EP3394271B1 (en) * | 2015-12-21 | 2020-05-20 | MolMed SpA | Stable integration of sin lentiviral transfer vectors |
EP3452102B1 (en) * | 2016-04-15 | 2024-06-12 | The Trustees of The University of Pennsylvania | Gene therapy for treating hemophilia a |
CN105950664B (zh) * | 2016-05-17 | 2019-03-29 | 上海优卡迪生物医药科技有限公司 | 一种靶向cd123的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用 |
CN105950663B (zh) * | 2016-05-17 | 2019-04-02 | 上海优卡迪生物医药科技有限公司 | 一种靶向cd30的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用 |
CN105950662B (zh) * | 2016-05-17 | 2019-04-30 | 上海优卡迪生物医药科技有限公司 | 一种靶向cd22的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用 |
CN105969805B (zh) * | 2016-05-17 | 2019-03-29 | 上海优卡迪生物医药科技有限公司 | 一种靶向Mesothelin的复制缺陷性重组慢病毒CAR-T转基因载体及其构建方法和应用 |
GB201706121D0 (en) * | 2017-04-18 | 2017-05-31 | Glaxosmithkline Ip Dev Ltd | Stable cell lines for retroviral production |
GB201715052D0 (en) | 2017-09-19 | 2017-11-01 | Oxford Genetics Ltd | Vectors |
EP3696272A1 (en) * | 2017-12-22 | 2020-08-19 | Oxford BioMedica (UK) Limited | Retroviral vector |
WO2019134866A1 (en) | 2018-01-03 | 2019-07-11 | Molmed Spa | Chimeric antigen receptors containing optimal spacer region |
EP3802796A1 (en) | 2018-05-30 | 2021-04-14 | Glycostem Therapeutics B.V. | Car nk cells |
EP3854879A4 (en) * | 2018-09-20 | 2022-06-22 | National University Corporation Tokyo Medical and Dental University | METHOD OF INCREASING THE PRODUCTION OF LENTIVIRUS VECTORS |
CN110184300A (zh) * | 2019-05-21 | 2019-08-30 | 安徽环球基因科技有限公司 | 一种利用稳定细胞株生产膜蛋白的方法 |
CN110982842B (zh) * | 2019-12-31 | 2023-04-18 | 山西大学 | 一种慢病毒表达载体的设计及应用 |
KR20230056669A (ko) | 2020-07-27 | 2023-04-27 | 안자리움 바이오사이언시스 아게 | Dna 분자의 조성물, 이의 제조 방법 및 이의 사용 방법 |
AU2022371427A1 (en) * | 2021-10-19 | 2024-03-14 | Amgen Inc. | Composition and methods for recombinant lentiviral production |
GB202213936D0 (en) * | 2022-09-23 | 2022-11-09 | Imperial College Innovations Ltd | Introns |
CN116731974A (zh) * | 2023-05-23 | 2023-09-12 | 杭州荣谷生物科技有限公司 | 病毒的制备方法和应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6013516A (en) * | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
IT1291135B1 (it) * | 1997-04-08 | 1998-12-29 | Angeletti P Ist Richerche Bio | Vettori ricombinanti utilizzabili in terapia genica |
CA2344208A1 (en) * | 2001-04-30 | 2002-10-30 | Oxford Biomedica (Uk) Limited | Method |
RU2312896C2 (ru) * | 2001-09-20 | 2007-12-20 | Глаксо Груп Лимитед | Последовательность нуклеиновой кислоты, кодирующая белок gag вич-1, способ получения указанной последовательности, вектор, содержащий ее, белок, кодируемый ею, фармацевтическая композиция и их применение для профилактики и/или лечения вич-инфекции и спида |
GB0220467D0 (en) * | 2002-09-03 | 2002-10-09 | Oxford Biomedica Ltd | Composition |
US7090837B2 (en) * | 2003-01-21 | 2006-08-15 | The Salk Institute For Biological Studies | Compositions and methods for tissue specific targeting of lentivirus vectors |
WO2005072364A2 (en) * | 2004-01-27 | 2005-08-11 | University Of Florida | A modified baculovirus expression system for production of pseudotyped raav vector |
WO2009104964A1 (en) * | 2008-02-19 | 2009-08-27 | Amsterdam Molecular Therapeutics B.V. | Optimisation of expression of parvoviral rep and cap proteins in insect cells |
CA2809447C (en) | 2010-09-02 | 2019-12-03 | Molmed S.P.A. | Semi-stable production of lentiviral vectors |
CN103228790B (zh) * | 2010-09-02 | 2015-08-19 | 莫尔梅德股份有限公司 | 慢病毒载体的稳定生产 |
CA2848939A1 (en) | 2011-10-05 | 2013-04-11 | Molmed Spa | Viral vectors purification system |
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2011
- 2011-09-01 CN CN201180042473.2A patent/CN103228790B/zh not_active Expired - Fee Related
- 2011-09-01 BR BR112013005139A patent/BR112013005139A2/pt not_active Application Discontinuation
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- 2011-09-01 WO PCT/EP2011/065090 patent/WO2012028681A1/en active Application Filing
- 2011-09-01 BR BR112013005120-5A patent/BR112013005120A2/pt not_active IP Right Cessation
- 2011-09-01 NZ NZ607686A patent/NZ607686A/en not_active IP Right Cessation
- 2011-09-01 PT PT117498493T patent/PT2480677E/pt unknown
- 2011-09-01 NZ NZ607690A patent/NZ607690A/en not_active IP Right Cessation
- 2011-09-01 RU RU2013114444/10A patent/RU2577979C2/ru active
- 2011-09-01 PL PL11752530T patent/PL2480678T3/pl unknown
- 2011-09-01 ES ES11749849.3T patent/ES2537184T3/es active Active
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- 2011-09-01 JP JP2013526470A patent/JP5897577B2/ja not_active Expired - Fee Related
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- 2011-09-01 ES ES11752530.3T patent/ES2460947T3/es active Active
- 2011-09-01 US US13/819,991 patent/US9441245B2/en active Active
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- 2011-09-01 CA CA2809453A patent/CA2809453C/en not_active Expired - Fee Related
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- 2011-09-01 AU AU2011298333A patent/AU2011298333B2/en not_active Ceased
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