HK1187953A1 - 慢病毒載體的穩定生產 - Google Patents

慢病毒載體的穩定生產

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Publication number
HK1187953A1
HK1187953A1 HK14100990.4A HK14100990A HK1187953A1 HK 1187953 A1 HK1187953 A1 HK 1187953A1 HK 14100990 A HK14100990 A HK 14100990A HK 1187953 A1 HK1187953 A1 HK 1187953A1
Authority
HK
Hong Kong
Prior art keywords
lentiviral vectors
stable production
stable
production
lentiviral
Prior art date
Application number
HK14100990.4A
Other languages
English (en)
Inventor
Chiara Bovolenta
Anna Stornaiuolo
Paolo Rizzardi
Fulvio Mavilio
Original Assignee
Molmed Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molmed Spa filed Critical Molmed Spa
Publication of HK1187953A1 publication Critical patent/HK1187953A1/zh

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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C12N15/8673—Special methods for packaging systems
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/21—Retroviridae, e.g. equine infectious anemia virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14144—Chimeric viral vector comprising heterologous viral elements for production of another viral vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C12N2740/16052—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14311—Parvovirus, e.g. minute virus of mice
    • C12N2750/14351—Methods of production or purification of viral material
    • C12N2750/14352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/60—Vectors comprising as targeting moiety peptide derived from defined protein from viruses
    • C12N2810/6045—RNA rev transcr viruses
    • C12N2810/6054—Retroviridae

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  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Control Of Eletrric Generators (AREA)
HK14100990.4A 2010-09-02 2014-01-29 慢病毒載體的穩定生產 HK1187953A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10175088 2010-09-02
PCT/EP2011/065090 WO2012028681A1 (en) 2010-09-02 2011-09-01 Stable production of lentiviral vectors

Publications (1)

Publication Number Publication Date
HK1187953A1 true HK1187953A1 (zh) 2014-04-17

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Application Number Title Priority Date Filing Date
HK13112180.0A HK1184822A1 (zh) 2010-09-02 2013-10-29 慢病毒載體的半穩定生產
HK14100990.4A HK1187953A1 (zh) 2010-09-02 2014-01-29 慢病毒載體的穩定生產

Family Applications Before (1)

Application Number Title Priority Date Filing Date
HK13112180.0A HK1184822A1 (zh) 2010-09-02 2013-10-29 慢病毒載體的半穩定生產

Country Status (23)

Country Link
US (3) US9441245B2 (zh)
EP (1) EP2480678B1 (zh)
JP (1) JP5897577B2 (zh)
KR (1) KR101990068B1 (zh)
CN (1) CN103228790B (zh)
AU (1) AU2011298333B2 (zh)
BR (2) BR112013005120A2 (zh)
CA (1) CA2809453C (zh)
CY (1) CY1115053T1 (zh)
DK (2) DK2480678T3 (zh)
ES (2) ES2460947T3 (zh)
HK (2) HK1184822A1 (zh)
HR (1) HRP20140392T1 (zh)
IL (2) IL224520A (zh)
NZ (2) NZ607690A (zh)
PL (1) PL2480678T3 (zh)
PT (2) PT2480678E (zh)
RS (1) RS53344B (zh)
RU (2) RU2577979C2 (zh)
SI (1) SI2480678T1 (zh)
SM (1) SMT201400058B (zh)
WO (1) WO2012028681A1 (zh)
ZA (1) ZA201300998B (zh)

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KR101990068B1 (ko) * 2010-09-02 2019-06-17 몰메드 에스피에이 렌티바이러스 벡터의 안정한 제조
US20140248695A1 (en) 2011-10-05 2014-09-04 MolMed, S.p.A. Viral Vectors Purification System
DK3889173T3 (da) 2013-02-15 2023-10-09 Bioverativ Therapeutics Inc Optimeret faktor viii-gen
WO2016196499A1 (en) * 2015-05-29 2016-12-08 Clark Atlanta University Human cell lines mutant for zic2
RU2752498C2 (ru) * 2015-11-24 2021-07-28 Глэксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед Стабильные клеточные линии для продуцирования ретровирусов
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JP2018537990A (ja) * 2015-12-21 2018-12-27 モルメド エスピーエー Ｓｉｎトランスファーベクターの安定した組み込み
KR102450833B1 (ko) 2016-04-15 2022-10-05 더 트러스티스 오브 더 유니버시티 오브 펜실베니아 혈우병 a 치료용 유전자 요법
CN105969805B (zh) * 2016-05-17 2019-03-29 上海优卡迪生物医药科技有限公司 一种靶向Mesothelin的复制缺陷性重组慢病毒CAR-T转基因载体及其构建方法和应用
CN105950664B (zh) * 2016-05-17 2019-03-29 上海优卡迪生物医药科技有限公司 一种靶向cd123的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用
CN105950663B (zh) * 2016-05-17 2019-04-02 上海优卡迪生物医药科技有限公司 一种靶向cd30的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用
CN105950662B (zh) * 2016-05-17 2019-04-30 上海优卡迪生物医药科技有限公司 一种靶向cd22的复制缺陷性重组慢病毒car-t转基因载体及其构建方法和应用
GB201706121D0 (en) 2017-04-18 2017-05-31 Glaxosmithkline Ip Dev Ltd Stable cell lines for retroviral production
GB201715052D0 (en) 2017-09-19 2017-11-01 Oxford Genetics Ltd Vectors
ES2951857T3 (es) * 2017-12-22 2023-10-25 Oxford Biomedica Ltd Vector retroviral
WO2019134866A1 (en) 2018-01-03 2019-07-11 Molmed Spa Chimeric antigen receptors containing optimal spacer region
WO2019229109A1 (en) 2018-05-30 2019-12-05 Glycostem Therapeutics B.V. Car nk cells
EP3854879A4 (en) * 2018-09-20 2022-06-22 National University Corporation Tokyo Medical and Dental University METHOD OF INCREASING THE PRODUCTION OF LENTIVIRUS VECTORS
CN110184300A (zh) * 2019-05-21 2019-08-30 安徽环球基因科技有限公司 一种利用稳定细胞株生产膜蛋白的方法
CN110982842B (zh) * 2019-12-31 2023-04-18 山西大学 一种慢病毒表达载体的设计及应用
CN116234917A (zh) 2020-07-27 2023-06-06 安杰瑞姆生物科学公司 Dna分子组合物及其制备方法和使用方法
WO2023069998A1 (en) * 2021-10-19 2023-04-27 Amgen Inc. Composition and methods for recombinant lentiviral production
GB202213936D0 (en) * 2022-09-23 2022-11-09 Imperial College Innovations Ltd Introns
CN116731974A (zh) * 2023-05-23 2023-09-12 杭州荣谷生物科技有限公司 病毒的制备方法和应用

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KR101990068B1 (ko) * 2010-09-02 2019-06-17 몰메드 에스피에이 렌티바이러스 벡터의 안정한 제조
KR101891626B1 (ko) 2010-09-02 2018-09-28 몰메드 에스피에이 렌티바이러스 벡터의 반-안정성 산물
US20140248695A1 (en) 2011-10-05 2014-09-04 MolMed, S.p.A. Viral Vectors Purification System

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DK2480678T3 (da) 2014-05-12
US20190284574A1 (en) 2019-09-19
AU2011298333B2 (en) 2015-05-14
RU2013114444A (ru) 2014-10-10
ZA201300998B (en) 2014-04-30
US10144937B2 (en) 2018-12-04
SI2480678T1 (sl) 2014-06-30
RU2013114445A (ru) 2014-10-10
PL2480678T3 (pl) 2014-08-29
RU2577982C2 (ru) 2016-03-20
HK1184822A1 (zh) 2014-01-30
BR112013005139A2 (pt) 2016-06-28
CN103228790A (zh) 2013-07-31
CY1115053T1 (el) 2016-12-14
US20140051125A1 (en) 2014-02-20
HRP20140392T1 (hr) 2014-06-06
NZ607686A (en) 2014-10-31
IL224521A (en) 2016-06-30
NZ607690A (en) 2014-10-31
IL224520A (en) 2016-06-30
JP5897577B2 (ja) 2016-03-30
RU2577979C2 (ru) 2016-03-20
KR20130101527A (ko) 2013-09-13
EP2480678B1 (en) 2014-02-12
US9441245B2 (en) 2016-09-13
CN103228790B (zh) 2015-08-19
RS53344B (en) 2014-10-31
WO2012028681A1 (en) 2012-03-08
ES2460947T3 (es) 2014-05-16
PT2480678E (pt) 2014-05-07
JP2013537801A (ja) 2013-10-07
US20180002721A1 (en) 2018-01-04
CA2809453C (en) 2019-12-03
PT2480677E (pt) 2015-07-13
BR112013005120A2 (pt) 2021-04-20
CA2809453A1 (en) 2012-03-08
SMT201400058B (it) 2014-07-07
DK2480677T3 (en) 2015-06-15
EP2480678A1 (en) 2012-08-01
KR101990068B1 (ko) 2019-06-17
AU2011298333A1 (en) 2013-03-21

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Effective date: 20210901