CY1105315T1 - Χρηση διπλης-κβαντικης 1η-nmr φασματοσκοπιας για την αναγνωριση συνδεσμικων αλληλεπιδρωντων με βιομορια στοχους - Google Patents

Χρηση διπλης-κβαντικης 1η-nmr φασματοσκοπιας για την αναγνωριση συνδεσμικων αλληλεπιδρωντων με βιομορια στοχους

Info

Publication number
CY1105315T1
CY1105315T1 CY20061100322T CY061100322T CY1105315T1 CY 1105315 T1 CY1105315 T1 CY 1105315T1 CY 20061100322 T CY20061100322 T CY 20061100322T CY 061100322 T CY061100322 T CY 061100322T CY 1105315 T1 CY1105315 T1 CY 1105315T1
Authority
CY
Cyprus
Prior art keywords
quantum
double
target
biomoles
nmr spectroscopy
Prior art date
Application number
CY20061100322T
Other languages
English (en)
Inventor
Claudio Dalvit
Carlo Battistini
Paolo Caccia
Patrizia Giordano
Paolo Pevarello
Michael Sundstrom
Marco Tato'
Original Assignee
Pharmacia Italia S.P.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Italia S.P.A. filed Critical Pharmacia Italia S.P.A.
Publication of CY1105315T1 publication Critical patent/CY1105315T1/el

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/46—NMR spectroscopy
    • G01R33/4625—Processing of acquired signals, e.g. elimination of phase errors, baseline fitting, chemometric analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
    • G01R33/00—Arrangements or instruments for measuring magnetic variables
    • G01R33/20—Arrangements or instruments for measuring magnetic variables involving magnetic resonance
    • G01R33/44—Arrangements or instruments for measuring magnetic variables involving magnetic resonance using nuclear magnetic resonance [NMR]
    • G01R33/46—NMR spectroscopy
    • G01R33/4633—Sequences for multi-dimensional NMR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Signal Processing (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Peptides Or Proteins (AREA)
  • Testing Or Measuring Of Semiconductors Or The Like (AREA)
  • Spectrometry And Color Measurement (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει μία ταχεία και αποδοτική διαδικασία σκριναρίσματος για αναγνώριση και παροχή χημικών ενώσεων οι οποίες αλληλεπιδρούν με βιομόρια στόχους, για παράδειγμα ένα θεραπευτικό βιομόριο στόχο όπως είναι μία πρωτεΐνη, όπου η εν λόγω διαδικασία περιλαμβάνει την γένεση δύο-διαστάσεων πρωτονίου (επανεστιασμένου ή όχι) διπλού-κβαντικού ή διπλού-κβαντικού χαλάρωσης ζυγισμένου διπλών κβαντικών-φασμάτων ενός μείγματος ενώσεων με και χωρίς το βιομόριο στόχο, έτσι ώστε να ανιχνευτούν οτιδήποτε διαφορές μεταξύ των φασμάτων οι οποίες αναγνωρίζουν την παρουσία μιας ή περισσότερων χημικών ενώσεων οι οποίες δεσμεύονται στο βιομόριο στόχο.
CY20061100322T 1999-10-29 2006-03-08 Χρηση διπλης-κβαντικης 1η-nmr φασματοσκοπιας για την αναγνωριση συνδεσμικων αλληλεπιδρωντων με βιομορια στοχους CY1105315T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9925677.8A GB9925677D0 (en) 1999-10-29 1999-10-29 Use of double-quantum 1H-NMR spectroscopy for the identification of ligands interacting with target biomolecules
PCT/EP2000/010370 WO2001033243A1 (en) 1999-10-29 2000-10-19 Use of double-quantum 1h-nmr spectroscopy for the identification of ligands interacting with target biomolecules

Publications (1)

Publication Number Publication Date
CY1105315T1 true CY1105315T1 (el) 2010-03-03

Family

ID=10863638

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20061100322T CY1105315T1 (el) 1999-10-29 2006-03-08 Χρηση διπλης-κβαντικης 1η-nmr φασματοσκοπιας για την αναγνωριση συνδεσμικων αλληλεπιδρωντων με βιομορια στοχους

Country Status (11)

Country Link
EP (1) EP1224486B1 (el)
JP (1) JP2003513290A (el)
AT (1) ATE314660T1 (el)
AU (1) AU780921B2 (el)
CA (1) CA2388216A1 (el)
CY (1) CY1105315T1 (el)
DE (1) DE60025246T2 (el)
DK (1) DK1224486T3 (el)
ES (1) ES2254245T3 (el)
GB (1) GB9925677D0 (el)
WO (1) WO2001033243A1 (el)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO20013387A (no) * 2001-07-09 2002-12-16 Leiv Eiriksson Nyfotek As Fremgangsmåte for måling av ulike bestanddeler i organisk og biologisk materiale
EP1511988A4 (en) * 2002-06-05 2005-07-06 Pharmacia & Upjohn Co Llc METHOD OF IDENTIFYING LIGANDS USING 1H NMR COMPETITIVE BINDING EXPERIMENTS
KR100901309B1 (ko) * 2002-06-15 2009-06-09 크리스탈지노믹스(주) 단백질의 활성 부위에 결합하는 화합물을 선별하는 방법
NO20025738D0 (no) * 2002-11-29 2002-11-29 Amersham Health As Metode
GB0308586D0 (en) * 2003-04-14 2003-05-21 Amersham Health R & D Ab Method and arrangements in NMR spectroscopy
EP2994143A4 (en) 2013-05-08 2017-02-01 Board of Regents, The University of Texas System Stat6 inhibitors
CN104181186B (zh) * 2014-09-03 2016-08-24 山西大学 一种黄芪注射液1h-nmr指纹图谱的构建方法
CN105823788B (zh) * 2016-05-12 2017-10-17 山西大学 山西老陈醋1h‑nmr指纹图谱的构建方法和应用
CN107102021A (zh) * 2017-05-03 2017-08-29 山西广誉远国药有限公司 一种海马1h‑nmr指纹图谱的构建方法和用途
CN114624276B (zh) * 2022-03-14 2023-03-24 中国科学院精密测量科学与技术创新研究院 氢检测半整数四极核高分辨固体nmr异核相关谱方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU711092B2 (en) * 1995-11-14 1999-10-07 Abbvie Inc. Use of nuclear magnetic resonance to design ligands to target biomolecules
US5698401A (en) * 1995-11-14 1997-12-16 Abbott Laboratories Use of nuclear magnetic resonance to identify ligands to target biomolecules
US5891643A (en) * 1995-11-14 1999-04-06 Abbott Laboratories Use of nuclear magnetic resonance to design ligands to target biomolecules
DE19649359C1 (de) * 1996-11-28 1998-02-12 Thomas Prof Dr Peters Verfahren zum Nachweis biologisch aktiver Substanzen in Substanzbibliotheken

Also Published As

Publication number Publication date
DE60025246D1 (de) 2006-02-02
ES2254245T3 (es) 2006-06-16
DK1224486T3 (da) 2006-02-13
WO2001033243A1 (en) 2001-05-10
AU780921B2 (en) 2005-04-28
GB9925677D0 (en) 1999-12-29
JP2003513290A (ja) 2003-04-08
EP1224486B1 (en) 2005-12-28
DE60025246T2 (de) 2006-09-07
ATE314660T1 (de) 2006-01-15
CA2388216A1 (en) 2001-05-10
EP1224486A1 (en) 2002-07-24
AU1142601A (en) 2001-05-14

Similar Documents

Publication Publication Date Title
CY1105315T1 (el) Χρηση διπλης-κβαντικης 1η-nmr φασματοσκοπιας για την αναγνωριση συνδεσμικων αλληλεπιδρωντων με βιομορια στοχους
TR199902167T2 (xx) Hedef biyomolek�llere ba�lanan ligandlar�n tan�nmas� i�in tek boyutlu n�kleer manyetik rezonans�n kullan�lmas�.
Moritz Tubulin or not tubulin: heading toward total protein staining as loading control in western blots
Mommen et al. Expanding the detectable HLA peptide repertoire using electron-transfer/higher-energy collision dissociation (EThcD)
Dallongeville et al. Proteins in art, archaeology, and paleontology: from detection to identification
CA2237343A1 (en) Use of nuclear magnetic resonance to identify ligands to target biomolecules
CY1111966T1 (el) Αντισωματα που δεσμευονται με κυτταροσυνδετο ca 125/0722ρ και μεθοδοι χρησεις αυτων
CY1109335T1 (el) Κιτ ραδιοεπισημανσης και δοκιμασια δεσμευσης
Tan et al. Direct quantitation of MHC‐bound peptide epitopes by selected reaction monitoring
ATE437185T1 (de) Gegen das gemeinsame epitop von mutierten p53 gerichtete humane synthetische einzelkettenantikörper und anwendungen davon
CY1112101T1 (el) Νευροτροφικοι παραγοντες
CY1112631T1 (el) ΜΕΘΟΔΟΙ ΤΑΥΤΟΠΟΙΗΣΗΣ ΟΓΚΩΝ ΠΟΥ ΑΠΟΚΡΙΝΟΝΤΑΙ ΣΕ ΑΓΩΓΗ ΜΕ ΑΝΤΙΣΩΜΑΤΑ ΑΝΤΙ-ΕrbB2
ATE489108T1 (de) Flt4 (vegfr-3) als ziel für tumornachweis (imaging) und antitumortherapie
CY1109699T1 (el) Νουκλεινικα οξεα που κωδικοποιουν πολυπεπτιδια πολυεπιτοπων
DE69940067D1 (de) Ger einheiten die zielmaterialbindende farbstoffe gebrauchen
ATE337011T1 (de) Modifizierte psma-liganden und deren verwendung
DK1358458T3 (da) Massedefektmærkning til bestemmelse af oligomersekvenser
ATE359300T1 (de) Flt4 (vegfr-3) als ein ziel für krebs darstellung und anti-krebs-behandlung
AU2003224674A8 (en) Compounds and methods for analyzing the proteome
ATE421584T1 (de) Cpg rezeptor (cpg-r) und darauf bezogene methoden
DK161112C (da) Baeremateriale til anvendelse ved immunbestemmelse,fremgangsmaade til fremstilling af baerematerialet og anvendelse deraf ved immunbestemmelser
CY1107474T1 (el) Χρηση 13c-nmr για να ανιχνευεται συνδεση
Vallée et al. Chemoselective bioconjugation of triazole phosphonites in aqueous media
ES2141249T3 (es) Procedimiento para la identificacion de celulas humanas y animales que presentan la capacidad para una proliferacion ilimitada o para una formacion de tumores.
HUP9800225A2 (hu) Eljárás és vegyületek analitok meghatározására remanenciaméréssel és ezek alkalmazásai