CY1105063T1 - Η χρηση της διepγασιας χτενισματος για την αναγνωριση των σημειων εναρξης αναδιπλασιασμου του dna - Google Patents

Η χρηση της διepγασιας χτενισματος για την αναγνωριση των σημειων εναρξης αναδιπλασιασμου του dna

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Publication number
CY1105063T1
CY1105063T1 CY20061100908T CY061100908T CY1105063T1 CY 1105063 T1 CY1105063 T1 CY 1105063T1 CY 20061100908 T CY20061100908 T CY 20061100908T CY 061100908 T CY061100908 T CY 061100908T CY 1105063 T1 CY1105063 T1 CY 1105063T1
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CY
Cyprus
Prior art keywords
replication
dna
initiation sites
genome
combing
Prior art date
Application number
CY20061100908T
Other languages
English (en)
Inventor
Aaron Bensimon
John Herrick
Olivier Hyrien
Original Assignee
Institut Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur filed Critical Institut Pasteur
Publication of CY1105063T1 publication Critical patent/CY1105063T1/el

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Τα ευκαρυωτικά γονιδιώματα διπλασιάζονται από την ενεργοποίηση πολλαπλών αμφίδρομων σημείων έναρξης του αναδιπλασιασμού. Τα προγράμματα αναδιπλασιασμού αυτών των κυττάρων εξαρτώνται από τη περιστασιακή και χωροταξική οργάνωση των σημείων έναρξης του αναδιπλασιασμού σε όλη την έκταση του γονιδιώματος. Για να ερευνηθεί το πρόγραμμα του αναδιπλασιασμού σε ένα ανώτερο ευκαρυωτικό, χρησιμοποιήσαμε μια τεχνική που ονομάζεται μοριακό χτένισμα. Αυτή η τεχνική επιτρέπει μια ποσοτική ανάλυση του αναδιπλασιασμού του DNA ενός γονιδιώματος ευρείας βάσης. Σαν σύστημα μοντέλο, η χρωματίνη του σπέρματος του Xenopus laevis σημάνθηκε διαφορικά σε διαδοχικά χρονικά σημεία μετά την έναρξη της σύνθεσης του DNA. Το γενωμικό DNA στη συνέχεια εξήχθη και χτενίστηκε επί μιας γυάλινης επιφάνειας. Απ' ευθείας μετρήσεις που πραγματοποιήθηκαν επί του σημασμένου DNA, παρείχαν μια κατανοητή ανάλυση της χωροταξικής και περιστασιακής οργάνωσης του προγράμματος αναδιπλασιασμού του πρώιμου εμβρύου του Χ. laevis και αποκάλυψαν ότι ο αριθμός των σημείων έναρξης αναδιπλασιασμού που ενεργοποιείται ανά κιλοβάσεις, αυξάνει σε όλη τη χρονική έκταση της σύνθεσης του DNA.
CY20061100908T 1999-05-28 2006-07-03 Η χρηση της διepγασιας χτενισματος για την αναγνωριση των σημειων εναρξης αναδιπλασιασμου του dna CY1105063T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13679299P 1999-05-28 1999-05-28
PCT/IB2000/001240 WO2000073503A2 (en) 1999-05-28 2000-05-29 Use of the combing process for the identification of dna origins of replication

Publications (1)

Publication Number Publication Date
CY1105063T1 true CY1105063T1 (el) 2010-03-03

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Family Applications (1)

Application Number Title Priority Date Filing Date
CY20061100908T CY1105063T1 (el) 1999-05-28 2006-07-03 Η χρηση της διepγασιας χτενισματος για την αναγνωριση των σημειων εναρξης αναδιπλασιασμου του dna

Country Status (12)

Country Link
US (3) US6248537B1 (el)
EP (1) EP1192283B1 (el)
AT (1) ATE330035T1 (el)
AU (1) AU6590400A (el)
CA (1) CA2375534C (el)
CY (1) CY1105063T1 (el)
DE (1) DE60028768T2 (el)
DK (1) DK1192283T3 (el)
ES (1) ES2265962T3 (el)
IL (1) IL146796A0 (el)
PT (1) PT1192283E (el)
WO (1) WO2000073503A2 (el)

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Also Published As

Publication number Publication date
AU6590400A (en) 2000-12-18
US20050142593A1 (en) 2005-06-30
PT1192283E (pt) 2006-10-31
DE60028768D1 (de) 2006-07-27
US6248537B1 (en) 2001-06-19
US20110159490A1 (en) 2011-06-30
IL146796A0 (en) 2002-07-25
DK1192283T3 (da) 2006-09-11
EP1192283B1 (en) 2006-06-14
EP1192283A2 (en) 2002-04-03
DE60028768T2 (de) 2007-05-24
WO2000073503A2 (en) 2000-12-07
ES2265962T3 (es) 2007-03-01
WO2000073503A3 (en) 2002-02-07
CA2375534C (en) 2009-09-01
WO2000073503A9 (en) 2002-03-07
ATE330035T1 (de) 2006-07-15
CA2375534A1 (en) 2000-12-07

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