CU22666A1 - Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados - Google Patents

Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados

Info

Publication number
CU22666A1
CU22666A1 CU1996107A CU1996107A CU22666A1 CU 22666 A1 CU22666 A1 CU 22666A1 CU 1996107 A CU1996107 A CU 1996107A CU 1996107 A CU1996107 A CU 1996107A CU 22666 A1 CU22666 A1 CU 22666A1
Authority
CU
Cuba
Prior art keywords
dengue
expression
virus
recombinant
adns
Prior art date
Application number
CU1996107A
Other languages
English (en)
Inventor
Diaz Rayner Rodriguez
Dominguez Delfina Esperanza Rosario
Perera Gabriel Jesus Marquez
Campos Raisa Vazquez
Villasuso Valexys Vazquez
Tirado Maria Guadalupe Guzman
Jimenez Mayra Mune
Acosta Anabel Alvarez
Curbelo Carlos Augusto Sariol
Ramudo Susana Vazquez
Nieto Gerardo Enrique Guillen
Suarez Jose Garcia
Original Assignee
Inst De Medicina Tropical Pedro Kouri
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Medicina Tropical Pedro Kouri, Ct Ingenieria Genetica Biotech filed Critical Inst De Medicina Tropical Pedro Kouri
Priority to CU1996107A priority Critical patent/CU22666A1/es
Priority to BR9713540-2A priority patent/BR9713540A/pt
Priority to PCT/CU1997/000006 priority patent/WO1998023754A1/es
Priority to AU53973/98A priority patent/AU5397398A/en
Priority to CN97181246A priority patent/CN1258317A/zh
Publication of CU22666A1 publication Critical patent/CU22666A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24161—Methods of inactivation or attenuation
    • C12N2710/24162—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

La presente invención se relaciona con la rama de la biotecnología y con las técnicas de ADN recombinante y en particular con un procedimiento para la expresión del gen que codifica para la proteína de la envoltura de los virus del dengue seropositivos 2 y 4 en levaduras metilotróficas del genero Pichia. El objetivo es obtener un inmunógeno para vacunación en humanos. Para el virus dengue serotipo 2 se partió de la secuencia génica que codifica para la peoteína E del virus, a partir de la cepa A 15 aislada durante la epidemia de fiebre del dengue y dengue hemorrágico ocurrida en Cuba en el ano 1981. En el caso del virus Dengue serotipo 4 se partió de la secuencia génica que codifica para la proteína E del virus, a partir de la cepa 814669 aislada durante la epidemia de fiebre del dengue ocurrida en Dominica en el ano 1981.
CU1996107A 1996-11-25 1996-11-25 Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados CU22666A1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CU1996107A CU22666A1 (es) 1996-11-25 1996-11-25 Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados
BR9713540-2A BR9713540A (pt) 1996-11-25 1997-11-25 Procedimento para a expressão do gene do vìrus da dengue
PCT/CU1997/000006 WO1998023754A1 (es) 1996-11-25 1997-11-25 Procedimiento para la expresion de genes de los virus del dengue
AU53973/98A AU5397398A (en) 1996-11-25 1997-11-25 Process for the expression of genes of the dengue viruses
CN97181246A CN1258317A (zh) 1996-11-25 1997-11-25 登革病毒基因表达的方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1996107A CU22666A1 (es) 1996-11-25 1996-11-25 Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados

Publications (1)

Publication Number Publication Date
CU22666A1 true CU22666A1 (es) 2001-04-27

Family

ID=46093346

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1996107A CU22666A1 (es) 1996-11-25 1996-11-25 Procedimiento para la expresión de genes de los virus del dengue en la levadura pichia pastoris, adns recombinantes y microorganismos transformados

Country Status (5)

Country Link
CN (1) CN1258317A (es)
AU (1) AU5397398A (es)
BR (1) BR9713540A (es)
CU (1) CU22666A1 (es)
WO (1) WO1998023754A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23245A1 (es) 2001-07-16 2007-10-17 Inst De Medicina Tropical Pedr CADENAS QUIMéRICAS CODIFICANTES PARA PROTEINAS INDUCTORAS DE EFECTOS CONTRA VIRUS. PREPARADOS UTILIZANDO PROTEINAS QUIMéRICAS
JP4500049B2 (ja) * 2001-12-04 2010-07-14 バヴァリアン・ノルディック・アクティーゼルスカブ フラビウイルスｎｓ１サブユニットワクチン
CU23578A1 (es) 2005-09-16 2010-09-30 Ct Ingenieria Genetica Biotech Proteína de la cápsida del virus dengue inductora de respuesta protectora y composición vacunal
CU23586A1 (es) * 2005-11-22 2010-10-30 Ct Ingenieria Genetica Biotech Métodos y proteínas para el tratamiento profiláctico y/o terapéutico de los cuatro serotipos del virus de dengue y otros flavivirus
CU23630A1 (es) 2006-10-30 2011-02-24 Ct Ingenieria Genetica Biotech Moléculas peptídicas quiméricas con propiedades antivirales contra los virus de la familia flaviviridae
CN102363751A (zh) * 2011-03-24 2012-02-29 中山大学 登革病毒样颗粒及其制备方法与应用
CN103103293B (zh) * 2013-02-01 2014-04-16 中华人民共和国北京出入境检验检疫局 登革2型病毒纳米磁分离实时荧光定量pcr检测试剂盒及核苷酸序列

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69624815T2 (de) * 1995-05-24 2003-07-10 Hawaii Biotech Group Untereinheitsimpfstoff gegen flavivirus infektion

Also Published As

Publication number Publication date
CN1258317A (zh) 2000-06-28
WO1998023754A1 (es) 1998-06-04
BR9713540A (pt) 2000-10-24
AU5397398A (en) 1998-06-22

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