CU22666A1 - PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS - Google Patents

PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS

Info

Publication number
CU22666A1
CU22666A1 CU1996107A CU1996107A CU22666A1 CU 22666 A1 CU22666 A1 CU 22666A1 CU 1996107 A CU1996107 A CU 1996107A CU 1996107 A CU1996107 A CU 1996107A CU 22666 A1 CU22666 A1 CU 22666A1
Authority
CU
Cuba
Prior art keywords
dengue
expression
virus
recombinant
adns
Prior art date
Application number
CU1996107A
Other languages
Spanish (es)
Inventor
Diaz Rayner Rodriguez
Dominguez Delfina Esperanza Rosario
Perera Gabriel Jesus Marquez
Campos Raisa Vazquez
Villasuso Valexys Vazquez
Tirado Maria Guadalupe Guzman
Jimenez Mayra Mune
Acosta Anabel Alvarez
Curbelo Carlos Augusto Sariol
Ramudo Susana Vazquez
Nieto Gerardo Enrique Guillen
Suarez Jose Garcia
Original Assignee
Inst De Medicina Tropical Pedro Kouri
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Medicina Tropical Pedro Kouri, Ct Ingenieria Genetica Biotech filed Critical Inst De Medicina Tropical Pedro Kouri
Priority to CU1996107A priority Critical patent/CU22666A1/en
Priority to AU53973/98A priority patent/AU5397398A/en
Priority to BR9713540-2A priority patent/BR9713540A/en
Priority to CN97181246A priority patent/CN1258317A/en
Priority to PCT/CU1997/000006 priority patent/WO1998023754A1/en
Publication of CU22666A1 publication Critical patent/CU22666A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24161—Methods of inactivation or attenuation
    • C12N2710/24162—Methods of inactivation or attenuation by genetic engineering
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Virology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se relaciona con la rama de la biotecnología y con las técnicas de ADN recombinante y en particular con un procedimiento para la expresión del gen que codifica para la proteína de la envoltura de los virus del dengue seropositivos 2 y 4 en levaduras metilotróficas del genero Pichia. El objetivo es obtener un inmunógeno para vacunación en humanos. Para el virus dengue serotipo 2 se partió de la secuencia génica que codifica para la peoteína E del virus, a partir de la cepa A 15 aislada durante la epidemia de fiebre del dengue y dengue hemorrágico ocurrida en Cuba en el ano 1981. En el caso del virus Dengue serotipo 4 se partió de la secuencia génica que codifica para la proteína E del virus, a partir de la cepa 814669 aislada durante la epidemia de fiebre del dengue ocurrida en Dominica en el ano 1981.The present invention relates to the biotechnology branch and to recombinant DNA techniques and in particular to a method for the expression of the gene that encodes the envelope protein of seropositive dengue viruses 2 and 4 in methylotrophic yeasts of the genus Pichia. The goal is to obtain an immunogen for vaccination in humans. For the dengue serotype 2 virus, it was based on the gene sequence coding for the peotein E of the virus, from strain A 15 isolated during the dengue fever and hemorrhagic dengue fever epidemic that occurred in Cuba in 1981. In the case Dengue virus serotype 4 was based on the gene sequence that codes for the protein E of the virus, from strain 814669 isolated during the dengue fever epidemic that occurred in Dominica in 1981.

CU1996107A 1996-11-25 1996-11-25 PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS CU22666A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CU1996107A CU22666A1 (en) 1996-11-25 1996-11-25 PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS
AU53973/98A AU5397398A (en) 1996-11-25 1997-11-25 Process for the expression of genes of the dengue viruses
BR9713540-2A BR9713540A (en) 1996-11-25 1997-11-25 Procedure for the expression of the dengue virus gene
CN97181246A CN1258317A (en) 1996-11-25 1997-11-25 Process for expression of genes of dengue viruses
PCT/CU1997/000006 WO1998023754A1 (en) 1996-11-25 1997-11-25 Process for the expression of genes of the dengue viruses

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1996107A CU22666A1 (en) 1996-11-25 1996-11-25 PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS

Publications (1)

Publication Number Publication Date
CU22666A1 true CU22666A1 (en) 2001-04-27

Family

ID=46093346

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1996107A CU22666A1 (en) 1996-11-25 1996-11-25 PROCEDURE FOR THE EXPRESSION OF GENES OF DENGUE VIRUSES IN PICHIA PASTORIS LEAVE, RECOMBINANT ADNS AND TRANSFORMED MICROORGANISMS

Country Status (5)

Country Link
CN (1) CN1258317A (en)
AU (1) AU5397398A (en)
BR (1) BR9713540A (en)
CU (1) CU22666A1 (en)
WO (1) WO1998023754A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23245A1 (en) 2001-07-16 2007-10-17 Inst De Medicina Tropical Pedr CHEMICAL CODING CHAINS FOR INDUCTIVE PROTEINS OF VIRUS EFFECTS. PREPARED USING CHEMICAL PROTEINS
EP1456230A2 (en) * 2001-12-04 2004-09-15 Bavarian Nordic A/S Flavivirus ns1 subunit vaccine
CU23578A1 (en) 2005-09-16 2010-09-30 Ct Ingenieria Genetica Biotech VIRUS CAPSID PROTEIN DENGES INDUCTIVE PROTECTION RESPONSE AND VACCINE COMPOSITION
CU23586A1 (en) * 2005-11-22 2010-10-30 Ct Ingenieria Genetica Biotech METHODS AND PROTEINS FOR THE PROPHLACTIC AND / OR THERAPEUTIC TREATMENT OF THE FOUR SEROTYPES OF THE DENGUE VIRUS AND OTHER FLAVIVIRUS
CU23630A1 (en) 2006-10-30 2011-02-24 Ct Ingenieria Genetica Biotech CHEMERIC PEPTIDE MOLECULES WITH ANTIVIRAL PROPERTIES AGAINST VIRUSES OF THE FLAVIVIRIDAE FAMILY
CN102363751A (en) * 2011-03-24 2012-02-29 中山大学 Dengue virus (DENV)-like particle as well as preparation method and application thereof
CN103103293B (en) * 2013-02-01 2014-04-16 中华人民共和国北京出入境检验检疫局 Dengue 2 virus nano-magnetic separation real-time fluorescent quantitative PCR (polymerase chain reaction) test kit and nucleotide sequence

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037221A1 (en) * 1995-05-24 1996-11-28 Hawaii Biotechnology Group, Inc. Subunit vaccine against flavivirus infection

Also Published As

Publication number Publication date
BR9713540A (en) 2000-10-24
AU5397398A (en) 1998-06-22
CN1258317A (en) 2000-06-28
WO1998023754A1 (en) 1998-06-04

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