CO6731137A2 - Métodos para determinar la zigosidad en una muestra compuesta - Google Patents

Métodos para determinar la zigosidad en una muestra compuesta

Info

Publication number
CO6731137A2
CO6731137A2 CO13179010A CO13179010A CO6731137A2 CO 6731137 A2 CO6731137 A2 CO 6731137A2 CO 13179010 A CO13179010 A CO 13179010A CO 13179010 A CO13179010 A CO 13179010A CO 6731137 A2 CO6731137 A2 CO 6731137A2
Authority
CO
Colombia
Prior art keywords
nucleic acid
methods
nucleotide sequence
primers
composite sample
Prior art date
Application number
CO13179010A
Other languages
English (en)
Inventor
Chandra-Shekara Channabasavaradhya
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of CO6731137A2 publication Critical patent/CO6731137A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Los métodos para determinar la presencia o la ausencia de una secuencia de nucleótidos insertada en un sitio de inserción particular de un ácido nucleico incluyen: aislar ácido nucleico a partir de la muestra de tejidos compuesta; poner el ácido nucleico en contacto con un cebador directo capaz de ligarse al ácido nucleico corriente arriba del sitio de inserción, un primer cebador inverso específico a la secuencia de nucleótidos insertada y un segundo cebador inverso capaz de ligarse al ácido nucleico corriente abajo del sitio de inserción.Se pueden usar los cebadores para reproducir ácidos nucleicos entre los cebadores. Se pueden analizar los ácidos nucleicos reproducidos para determinar si una secuencia de nucleótidos insertada está presente o ausente en la muestra.
CO13179010A 2010-12-29 2013-07-29 Métodos para determinar la zigosidad en una muestra compuesta CO6731137A2 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201061428142P 2010-12-29 2010-12-29

Publications (1)

Publication Number Publication Date
CO6731137A2 true CO6731137A2 (es) 2013-08-15

Family

ID=46383837

Family Applications (1)

Application Number Title Priority Date Filing Date
CO13179010A CO6731137A2 (es) 2010-12-29 2013-07-29 Métodos para determinar la zigosidad en una muestra compuesta

Country Status (16)

Country Link
US (1) US20140017684A1 (es)
EP (1) EP2659006A4 (es)
CN (1) CN103403185A (es)
AR (1) AR084630A1 (es)
AU (2) AU2011352159A1 (es)
BR (1) BRPI1105703A2 (es)
CA (1) CA2822967A1 (es)
CL (1) CL2013001893A1 (es)
CO (1) CO6731137A2 (es)
MX (1) MX2013007573A (es)
NZ (1) NZ611916A (es)
RU (2) RU2605324C2 (es)
UA (1) UA112977C2 (es)
UY (1) UY33843A (es)
WO (1) WO2012092327A2 (es)
ZA (1) ZA201304475B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018100466A1 (en) * 2016-11-30 2018-06-07 Semiconductor Energy Laboratory Co., Ltd. Display device, display module, and electronic device
CN113543631A (zh) * 2019-03-28 2021-10-22 百塞生技有限责任公司 大豆转基因事件ind-øø41ø-5

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) * 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants
FR2796963B1 (fr) * 1999-07-28 2001-09-28 Rhobio Procede d'obtention de lignees isotransgeniques
PT1620571E (pt) * 2003-05-02 2015-09-03 Du Pont Milho do evento tc1507 e métodos para deteção deste
PT1708560E (pt) * 2003-12-15 2015-06-03 Monsanto Technology Llc Planta de milho mon88017 e composições e métodos para a sua detecção
US7323556B2 (en) * 2004-09-29 2008-01-29 Pioneer Hi-Bred International, Inc. Corn event DAS-59122-7 and methods for detection thereof
PT1885176T (pt) * 2005-05-27 2016-11-28 Monsanto Technology Llc Evento mon89788 de soja e métodos para a sua deteção
EP1910547A2 (en) * 2005-07-29 2008-04-16 Monsanto Technology, LLC Development of novel germplasm using segregates from transgenic crosses
CN101238217B (zh) * 2005-08-08 2016-01-20 拜尔作物科学公司 耐受除草剂的棉花植物和用于鉴定其的方法
AU2007227665A1 (en) * 2006-03-17 2007-09-27 Merck & Co., Inc. Method for genetic selection of high-plasmid producing E. coli clones
CN1873010B (zh) * 2006-04-14 2010-04-07 中国科学院武汉植物园 利用花生Ara h3启动子转基因载体的制备方法及应用
US7928295B2 (en) * 2006-08-24 2011-04-19 Bayer Bioscience N.V. Herbicide tolerant rice plants and methods for identifying same
EP2132320B1 (en) * 2007-04-05 2013-08-14 Bayer CropScience NV Insect resistant cotton plants and methods for identifying same
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
CN101680000B (zh) * 2007-06-11 2014-06-11 拜尔作物科学公司 包含优良事件ee-gh6的抗虫棉花植物及其鉴定方法
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass

Also Published As

Publication number Publication date
WO2012092327A3 (en) 2013-01-10
RU2605324C2 (ru) 2016-12-20
AR084630A1 (es) 2013-05-29
RU2013135397A (ru) 2015-02-10
CN103403185A (zh) 2013-11-20
WO2012092327A2 (en) 2012-07-05
US20140017684A1 (en) 2014-01-16
EP2659006A4 (en) 2014-10-29
UY33843A (es) 2012-07-31
NZ611916A (en) 2015-12-24
MX2013007573A (es) 2013-07-22
CA2822967A1 (en) 2012-07-05
AU2011352159A1 (en) 2013-07-04
AU2016262648A1 (en) 2016-12-08
CL2013001893A1 (es) 2013-12-06
RU2016144372A (ru) 2018-12-18
ZA201304475B (en) 2014-09-25
EP2659006A2 (en) 2013-11-06
BRPI1105703A2 (pt) 2015-08-04
UA112977C2 (uk) 2016-11-25

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