AR084630A1 - Metodos para determinar la zigosidad en una muestra compuesta - Google Patents

Metodos para determinar la zigosidad en una muestra compuesta

Info

Publication number
AR084630A1
AR084630A1 ARP110104981A ARP110104981A AR084630A1 AR 084630 A1 AR084630 A1 AR 084630A1 AR P110104981 A ARP110104981 A AR P110104981A AR P110104981 A ARP110104981 A AR P110104981A AR 084630 A1 AR084630 A1 AR 084630A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
methods
nucleotide sequence
zigosity
primers
Prior art date
Application number
ARP110104981A
Other languages
English (en)
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR084630A1 publication Critical patent/AR084630A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Los métodos para determinar la presencia o la ausencia de una secuencia de nucleótidos insertada en un sitio de inserción particular de un ácido nucleico incluyen: aislar ácido nucleico a partir de la muestra de tejidos compuesta; poner el ácido nucleico en contacto con un cebador directo capaz de ligarse al ácido nucleico corriente arriba del sitio de inserción, un primer cebador inverso específico a la secuencia de nucleótidos insertada y un segundo cebador inverso capaz de ligarse al ácido nucleico corriente abajo del sitio de inserción. Se pueden usar los cebadores para reproducir ácidos nucleicos entre los cebadores. Se pueden analizar los ácidos nucleicos reproducidos para determinar si una secuencia de nucleótidos insertada está presente o ausente en la muestra.
ARP110104981A 2010-12-29 2011-12-28 Metodos para determinar la zigosidad en una muestra compuesta AR084630A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201061428142P 2010-12-29 2010-12-29

Publications (1)

Publication Number Publication Date
AR084630A1 true AR084630A1 (es) 2013-05-29

Family

ID=46383837

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110104981A AR084630A1 (es) 2010-12-29 2011-12-28 Metodos para determinar la zigosidad en una muestra compuesta

Country Status (16)

Country Link
US (1) US20140017684A1 (es)
EP (1) EP2659006A4 (es)
CN (1) CN103403185A (es)
AR (1) AR084630A1 (es)
AU (2) AU2011352159A1 (es)
BR (1) BRPI1105703A2 (es)
CA (1) CA2822967A1 (es)
CL (1) CL2013001893A1 (es)
CO (1) CO6731137A2 (es)
MX (1) MX2013007573A (es)
NZ (1) NZ611916A (es)
RU (2) RU2605324C2 (es)
UA (1) UA112977C2 (es)
UY (1) UY33843A (es)
WO (1) WO2012092327A2 (es)
ZA (1) ZA201304475B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018100466A1 (en) * 2016-11-30 2018-06-07 Semiconductor Energy Laboratory Co., Ltd. Display device, display module, and electronic device
WO2020197558A1 (en) * 2019-03-28 2020-10-01 Bioceres Llc Soybean transgenic event ind-øø41ø-5

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) * 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants
FR2796963B1 (fr) * 1999-07-28 2001-09-28 Rhobio Procede d'obtention de lignees isotransgeniques
EP1620571B1 (en) * 2003-05-02 2015-07-01 Dow AgroSciences LLC Corn event tc1507 and methods for detection thereof
WO2005059103A2 (en) * 2003-12-15 2005-06-30 Monsanto Technology Llc Corn plant mon88017 and compositions and methods for detection thereof
AU2005292090B2 (en) * 2004-09-29 2011-02-03 Corteva Agriscience Llc Corn event DAS-59122-7 and methods for detection thereof
AP2693A (en) * 2005-05-27 2013-07-16 Monsanto Technology Llc Soybean event MON89788 and methods for detection thereof
WO2007015945A2 (en) * 2005-07-29 2007-02-08 Monsanto Technology Llc Development of novel germplasm using segregates from transgenic crosses
EP1922409B1 (en) * 2005-08-08 2017-11-08 Bayer CropScience NV Herbicide tolerant cotton plants and methods for identifying same
JP2009529875A (ja) * 2006-03-17 2009-08-27 メルク エンド カムパニー インコーポレーテッド プラスミド高生産性大腸菌クローンの遺伝子選択方法。
CN1873010B (zh) * 2006-04-14 2010-04-07 中国科学院武汉植物园 利用花生Ara h3启动子转基因载体的制备方法及应用
US7928295B2 (en) * 2006-08-24 2011-04-19 Bayer Bioscience N.V. Herbicide tolerant rice plants and methods for identifying same
CN103773863A (zh) * 2007-04-05 2014-05-07 拜尔作物科学公司 抗虫棉花植物及其鉴定方法
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
EP2615173B1 (en) * 2007-06-11 2020-09-16 Basf Agricultural Solutions Seed Us Llc Insect resistant cotton plants and methods for identifying same
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass

Also Published As

Publication number Publication date
RU2605324C2 (ru) 2016-12-20
WO2012092327A2 (en) 2012-07-05
WO2012092327A3 (en) 2013-01-10
AU2011352159A1 (en) 2013-07-04
AU2016262648A1 (en) 2016-12-08
NZ611916A (en) 2015-12-24
CN103403185A (zh) 2013-11-20
BRPI1105703A2 (pt) 2015-08-04
CL2013001893A1 (es) 2013-12-06
US20140017684A1 (en) 2014-01-16
UY33843A (es) 2012-07-31
CA2822967A1 (en) 2012-07-05
RU2013135397A (ru) 2015-02-10
CO6731137A2 (es) 2013-08-15
ZA201304475B (en) 2014-09-25
EP2659006A4 (en) 2014-10-29
UA112977C2 (uk) 2016-11-25
MX2013007573A (es) 2013-07-22
EP2659006A2 (en) 2013-11-06
RU2016144372A (ru) 2018-12-18

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