CO4410255A1 - Procedimiento para introducir acido nucleico en celulas eucarioticas superiores y medio para transfeccion - Google Patents
Procedimiento para introducir acido nucleico en celulas eucarioticas superiores y medio para transfeccionInfo
- Publication number
- CO4410255A1 CO4410255A1 CO95023182A CO95023182A CO4410255A1 CO 4410255 A1 CO4410255 A1 CO 4410255A1 CO 95023182 A CO95023182 A CO 95023182A CO 95023182 A CO95023182 A CO 95023182A CO 4410255 A1 CO4410255 A1 CO 4410255A1
- Authority
- CO
- Colombia
- Prior art keywords
- cells
- procedure
- polication
- transfection
- average
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000001890 transfection Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 abstract 5
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
1. PROCEDIMIENTO PARA INTRODUCIR COMPLEJOS DE A- DICO NUCLEICO/POLICATION EN CELULAS EUCARIOTICAS SUPERIORES AL INCUBAR LAS CELULAS EN UN MEDIO DE TRANSFECCION QUE CONTIENE COMO SU CONSTITUYENTE ACTIVO LOS COMPLEJOS DE ACIDO, NUCLEICO/POLICATION CARACTERIZADO POR AGREGAR AL MEDIO 10-15% DE GLI- CEROL Y/O ETILENGLICOL BASADO EN EL VOLUMEN TOTAL DEL MEDIO, APLICAR EL MEDIO DE TRANSFECCION A LAS CELULAS Y POR ESTAR PRESENTE EL GLICEROL/ETILEN- GLICOL A LO LARGO DE TODO EL PROCESO DE INCUBA- CION. 10. PROCEDIMIENTO PARA LA PREPARACION DE CELULAS QUE EXPRESAN UN POLIPEPTIDO HETEROLOGO DE UNA MO- LECULA DE ADN TRANSFECTADA ESTABLE, CARACTERIZADO PORQUE SE TRANSFECTAN CELULAS EN PRESENCIA DE GLI- CEROL Y/O ETILENGLICOL CON UN COMPLEJO A BASE DE UNA MOLECULA DE ADN QUE CODIFICA EL POLIPEPTIDO HETEROLOGO, Y UN POLICATION, QUE EVENTUALMENTE ES- TA CONJUGADO TOTAL O PARCIALMENTE CON UN FACTOR DE INTERNALIZACION, LAS CELULAS SE CULTIVAN, SE SE- LECCIONAN CLONES DE CELULAS ESTABLES Y EVENTUAL- MENTE SE ESTABLECEN LINEAS DE CELULAS A PARTIR DE LOS CLONES OBTENIDOS. .
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE4418965A DE4418965A1 (de) | 1994-05-31 | 1994-05-31 | Verfahren zum Einbringen von Nukleinsäure in höhere eukaryotische Zellen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO4410255A1 true CO4410255A1 (es) | 1997-01-09 |
Family
ID=6519414
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO95023182A CO4410255A1 (es) | 1994-05-31 | 1995-05-30 | Procedimiento para introducir acido nucleico en celulas eucarioticas superiores y medio para transfeccion |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5965404A (es) |
| EP (1) | EP0763124B1 (es) |
| JP (1) | JPH10500856A (es) |
| AT (1) | ATE258602T1 (es) |
| AU (1) | AU709019B2 (es) |
| CA (1) | CA2191762A1 (es) |
| CO (1) | CO4410255A1 (es) |
| DE (2) | DE4418965A1 (es) |
| MX (1) | MX9605942A (es) |
| TW (1) | TW496898B (es) |
| WO (1) | WO1995033061A1 (es) |
| ZA (1) | ZA954404B (es) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996021036A2 (en) * | 1994-12-30 | 1996-07-11 | Chiron Viagene, Inc. | Nucleic acid condensing agents with reduced immunogenicity |
| WO1999019502A1 (en) * | 1997-10-10 | 1999-04-22 | Aventis Pharma Deutschland Gmbh | Transfection system for the transfer of nucleic acids into cells |
| EP0967288A1 (en) * | 1998-06-16 | 1999-12-29 | Hoechst Marion Roussel Deutschland GmbH | Transfection system for the transfer of nucleic acids into cells |
| EP0908521A1 (en) * | 1997-10-10 | 1999-04-14 | Hoechst Marion Roussel Deutschland GmbH | Transfection system for the transfer of nucleic acids into cells |
| GB9811059D0 (en) * | 1998-05-23 | 1998-07-22 | Univ Strathclyde | Polyamino acid vesicles |
| GB9908195D0 (en) | 1999-04-09 | 1999-06-02 | Microbiological Res Authority | Treatment of intracellular infection |
| DE19952983A1 (de) * | 1999-11-03 | 2001-05-17 | Acgt Progenomics Ag | Verfahren zum Transfer von molekularen Substanzen mit prokaryontischen nukleinsäurebindenden Proteinen |
| DE10049010A1 (de) * | 2000-10-04 | 2002-04-18 | Boehringer Ingelheim Int | Transferrin-Polykation/DNS-Komplexe für die systemische Therapie von Tumorerkrankungen mit zytotoxischen Proteinen |
| WO2003078576A2 (en) * | 2002-03-12 | 2003-09-25 | Nitto Denko Corporation | Vector for transfection of eukaryotic cells |
| US7833513B2 (en) | 2004-12-03 | 2010-11-16 | Rhode Island Hospital | Treatment of Alzheimer's Disease |
| JP5864077B2 (ja) | 2006-09-08 | 2016-02-17 | ロード アイランド ホスピタル | アルコール誘発性肝疾患の治療、予防および回復 |
| JP2010502719A (ja) | 2006-09-08 | 2010-01-28 | ロード アイランド ホスピタル | アルコール誘発性脳疾患の治療、予防および回復 |
| SI3135301T1 (en) | 2010-06-22 | 2018-08-31 | Onxeo | OPTIMIZED SYSTEM OF DELIVERY AND VIVO WITH ENDOSOMOLITIC MEANS FOR KONJUGATE NUCLEIC ACIDS |
| US11807857B2 (en) | 2014-06-25 | 2023-11-07 | Monsanto Technology Llc | Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression |
| CN108138177B9 (zh) | 2015-07-23 | 2021-08-13 | 法国居里学院 | Dbait分子与PARP抑制剂的组合用于治疗癌症的用途 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4652522A (en) * | 1983-07-05 | 1987-03-24 | The University Of Pennsylvania | Continuous lymphocyte cell lines, their production and use |
| US4608339A (en) * | 1983-10-25 | 1986-08-26 | Yoakum George H | Protoplast fusion method for high-frequency DNA transfection in human cells |
| IL83348A (en) * | 1986-08-26 | 1995-12-08 | Du Pont | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| JPS63102682A (ja) * | 1986-10-20 | 1988-05-07 | Medeisa Shinyaku Kk | 真核細胞内へのｄｎａ導入法 |
| US5354844A (en) * | 1989-03-16 | 1994-10-11 | Boehringer Ingelheim International Gmbh | Protein-polycation conjugates |
| AU642889B2 (en) * | 1989-07-11 | 1993-11-04 | Biotechnology Research And Development Corporation | Aerosol beam microinjector |
| US5286634A (en) * | 1989-09-28 | 1994-02-15 | Stadler Joan K | Synergistic method for host cell transformation |
| CA2091346C (en) * | 1990-09-14 | 2007-04-24 | Philip Frost | Methods and compositions for genetic therapy and potentiation of anti-tumor immunity |
| NZ244306A (en) * | 1991-09-30 | 1995-07-26 | Boehringer Ingelheim Int | Composition for introducing nucleic acid complexes into eucaryotic cells, complex containing nucleic acid and endosomolytic agent, peptide with endosomolytic domain and nucleic acid binding domain and preparation |
| US5254342A (en) * | 1991-09-30 | 1993-10-19 | University Of Southern California | Compositions and methods for enhanced transepithelial and transendothelial transport or active agents |
| US5521291A (en) * | 1991-09-30 | 1996-05-28 | Boehringer Ingelheim International, Gmbh | Conjugates for introducing nucleic acid into higher eucaryotic cells |
| WO1993019768A1 (en) * | 1992-04-03 | 1993-10-14 | The Regents Of The University Of California | Self-assembling polynucleotide delivery system |
-
1994
- 1994-05-31 DE DE4418965A patent/DE4418965A1/de not_active Withdrawn
-
1995
- 1995-05-24 EP EP95920875A patent/EP0763124B1/de not_active Expired - Lifetime
- 1995-05-24 MX MX9605942A patent/MX9605942A/es unknown
- 1995-05-24 AT AT95920875T patent/ATE258602T1/de not_active IP Right Cessation
- 1995-05-24 WO PCT/EP1995/001969 patent/WO1995033061A1/de active IP Right Grant
- 1995-05-24 AU AU26158/95A patent/AU709019B2/en not_active Ceased
- 1995-05-24 JP JP8500272A patent/JPH10500856A/ja not_active Abandoned
- 1995-05-24 CA CA002191762A patent/CA2191762A1/en not_active Abandoned
- 1995-05-24 US US08/750,181 patent/US5965404A/en not_active Expired - Lifetime
- 1995-05-24 DE DE59510853T patent/DE59510853D1/de not_active Expired - Fee Related
- 1995-05-30 CO CO95023182A patent/CO4410255A1/es unknown
- 1995-05-30 ZA ZA954404A patent/ZA954404B/xx unknown
- 1995-05-31 TW TW084105508A patent/TW496898B/zh active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2191762A1 (en) | 1995-12-07 |
| EP0763124A1 (de) | 1997-03-19 |
| ZA954404B (en) | 1995-11-30 |
| JPH10500856A (ja) | 1998-01-27 |
| WO1995033061A1 (de) | 1995-12-07 |
| EP0763124B1 (de) | 2004-01-28 |
| TW496898B (en) | 2002-08-01 |
| AU709019B2 (en) | 1999-08-19 |
| AU2615895A (en) | 1995-12-21 |
| ATE258602T1 (de) | 2004-02-15 |
| US5965404A (en) | 1999-10-12 |
| DE4418965A1 (de) | 1995-12-07 |
| DE59510853D1 (de) | 2004-03-04 |
| MX9605942A (es) | 1997-12-31 |
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