CO2022004535A2 - Secuencia de extensión n-terminal para la expresión de péptidos terapéuticos recombinantes - Google Patents
Secuencia de extensión n-terminal para la expresión de péptidos terapéuticos recombinantesInfo
- Publication number
- CO2022004535A2 CO2022004535A2 CONC2022/0004535A CO2022004535A CO2022004535A2 CO 2022004535 A2 CO2022004535 A2 CO 2022004535A2 CO 2022004535 A CO2022004535 A CO 2022004535A CO 2022004535 A2 CO2022004535 A2 CO 2022004535A2
- Authority
- CO
- Colombia
- Prior art keywords
- expression
- terminal extension
- therapeutic peptides
- recombinant therapeutic
- extension sequence
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/605—Glucagons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
RESUMEN La invención se relaciona con secuencias de extensión N-terminal las cuales se emplean para mejorar la expresión de péptidos terapéuticos recombinantes. La invención también se relaciona con un proceso para la expresión de alto nivel de péptidos terapéuticos recombinantes usando la secuencia de extensión N-terminal. La invención también proporciona ácidos nucleicos, vectores y células hospederas recombinantes para la producción eficiente de proteínas biológicamente activas como lirapéptido.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IN201941009728 | 2019-09-13 | ||
PCT/IN2020/050790 WO2021048878A1 (en) | 2019-09-13 | 2020-09-12 | N-terminal extension sequence for expression of recombinant therapeutic peptides |
Publications (1)
Publication Number | Publication Date |
---|---|
CO2022004535A2 true CO2022004535A2 (es) | 2022-04-29 |
Family
ID=74866188
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CONC2022/0004535A CO2022004535A2 (es) | 2019-09-13 | 2022-04-08 | Secuencia de extensión n-terminal para la expresión de péptidos terapéuticos recombinantes |
Country Status (14)
Country | Link |
---|---|
US (1) | US20230002468A1 (es) |
EP (1) | EP4028519A4 (es) |
JP (1) | JP2022548598A (es) |
KR (1) | KR20220058631A (es) |
CN (1) | CN114651063A (es) |
AU (1) | AU2020345138A1 (es) |
BR (1) | BR112022004601A2 (es) |
CA (1) | CA3150902A1 (es) |
CO (1) | CO2022004535A2 (es) |
CU (1) | CU20220017A7 (es) |
IL (1) | IL291127A (es) |
MX (1) | MX2022003002A (es) |
WO (1) | WO2021048878A1 (es) |
ZA (1) | ZA202202579B (es) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4314034A2 (en) * | 2021-03-31 | 2024-02-07 | Biological E Limited | Constructs and methods for increased expression of polypeptides |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030082671A1 (en) * | 2001-07-24 | 2003-05-01 | Thomas Hoeg-Jensen | Method for making acylated polypeptides |
WO2011119484A1 (en) * | 2010-03-23 | 2011-09-29 | Iogenetics, Llc | Bioinformatic processes for determination of peptide binding |
SG11201405276PA (en) * | 2012-02-27 | 2014-10-30 | Amunix Operating Inc | Xten conjugate compositions and methods of making same |
US20190352365A1 (en) * | 2017-01-18 | 2019-11-21 | Savior Lifetec Corporation | Expression construct and method for producing proteins of interest |
WO2019082138A1 (en) * | 2017-10-27 | 2019-05-02 | Lorven Biologics Private Limited | PROCESS FOR THE PREPARATION OF LIRAGLUTIDE USING RECOMBINANT SACCHAROMYCES CEREVISIAE |
CN107881187A (zh) * | 2017-11-20 | 2018-04-06 | 珠海联邦制药股份有限公司 | 将大肠杆菌表达的融合蛋白转化为利拉鲁肽的制备方法及应用 |
CN108218997B (zh) * | 2017-12-30 | 2020-12-15 | 北京中科唯新生物医学研究所有限公司 | 一种用于纯化蛋白的表达载体 |
EP3741774A4 (en) * | 2018-01-19 | 2021-06-02 | Pepgene Inc. | N-TERMINAL FUSION PARTNER FOR THE MANUFACTURING OF A RECOMBINANT POLYPEPTID AND PROCESS FOR THE MANUFACTURING OF A RECOMBINANT POLYPEPTID USING THESE |
-
2020
- 2020-09-12 EP EP20863997.1A patent/EP4028519A4/en active Pending
- 2020-09-12 CU CU2022000017A patent/CU20220017A7/es unknown
- 2020-09-12 MX MX2022003002A patent/MX2022003002A/es unknown
- 2020-09-12 CA CA3150902A patent/CA3150902A1/en active Pending
- 2020-09-12 BR BR112022004601A patent/BR112022004601A2/pt unknown
- 2020-09-12 CN CN202080077076.8A patent/CN114651063A/zh active Pending
- 2020-09-12 US US17/763,785 patent/US20230002468A1/en active Pending
- 2020-09-12 WO PCT/IN2020/050790 patent/WO2021048878A1/en active Application Filing
- 2020-09-12 KR KR1020227012065A patent/KR20220058631A/ko active Search and Examination
- 2020-09-12 AU AU2020345138A patent/AU2020345138A1/en active Pending
- 2020-09-12 JP JP2022516303A patent/JP2022548598A/ja active Pending
-
2022
- 2022-03-02 ZA ZA2022/02579A patent/ZA202202579B/en unknown
- 2022-03-06 IL IL291127A patent/IL291127A/en unknown
- 2022-04-08 CO CONC2022/0004535A patent/CO2022004535A2/es unknown
Also Published As
Publication number | Publication date |
---|---|
EP4028519A1 (en) | 2022-07-20 |
US20230002468A1 (en) | 2023-01-05 |
AU2020345138A1 (en) | 2022-04-21 |
CA3150902A1 (en) | 2021-03-18 |
KR20220058631A (ko) | 2022-05-09 |
MX2022003002A (es) | 2022-04-07 |
WO2021048878A1 (en) | 2021-03-18 |
BR112022004601A2 (pt) | 2022-05-31 |
IL291127A (en) | 2022-05-01 |
EP4028519A4 (en) | 2023-10-11 |
ZA202202579B (en) | 2022-10-26 |
CN114651063A (zh) | 2022-06-21 |
CU20220017A7 (es) | 2022-10-11 |
JP2022548598A (ja) | 2022-11-21 |
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