Background technology
Termite is class existence and the extremely strong social insect of adaptive capacity.They are food with the fibrous material usually, and its harm relates to aspects such as building construction, means of transportation, telecommunication equipment, archives and books, textile, weapons and ammunitions, agriculture and forestry plant.Annual about 20~2,500,000,000 yuan of the economic loss that termite damage causes China.As for the breaking-up that termite is caused cultural relics and historic sites, dam reservoirs etc., its consequence then is difficult to weigh with economic data.
Cause harm for various termites, at present both at home and abroad based on chemical control.But the chemical agent that seldom control termites of aiming at is arranged in the world and develop.In fact the development process of termite preventing and controlling agent comprises a large amount of work that existing pesticide species is screened.For example, in the preventative improvement of termite, need the screening medicament strong, to improve the shield effectiveness of soil chemistry barrier to the termite anti-repelling properties.And for example, in termite-killing trapping control, need the suitable decoy of screening and add in the middle of the poison bait, in natural environment, find the time that poison bait is used to shorten termite, thereby improve whole trapping effect with debita spissitudo.Measuring the influence of reagent agent to the termite sensory system quickly and accurately, is the important prerequisite of screening operation success or not.
At present, is no lack of the instrument that the physiological reaction to the insect sensory system detects at scientific research field.But those detecting instrument complex structures, involve great expense, and complex operation based on bioelectrical activity; Not only basic unit's control unit makes inquiries for no reason at all, and its testing result also may not have direct directive significance to the medicament screening.
The utility model content
At the deficiencies in the prior art part, it is a kind of simple in structure and can accurately detect the checkout gear of termite to the food olfaction reaction that the utility model provides.
The utility model is for reaching above purpose, be to realize: a kind of termite olfaction reaction checking device is provided by such technical scheme, the box body that comprises upper end open, place at the upper end open place of box body can be with the loam cake of this box body sealing, on cover and be provided with air-vent, the bottom of being close to box body is provided with at least 2 tube connectors that communicate with the inner chamber of box body symmetrically, and the other end of every tube connector connects a closed container by flexible pipe; Box body, tube connector, closed container are all made by light transmissive material.
A kind of improvement as termite olfaction reaction checking device of the present utility model: the periphery at box body is provided with bracing frame, and tube connector is fixed on the bracing frame.
Further improvement as termite olfaction reaction checking device of the present utility model: closed container is by the transparent bottle and be positioned at this sealing-plug of bleeding bottle bottleneck and form of bleeding; One end of flexible pipe links to each other with tube connector, and bleed therewith the first half of bottle of the other end links to each other.
Further improvement as termite olfaction reaction checking device of the present utility model: the bottom at box body and bracing frame is provided with pedestal.
Termite olfaction reaction checking device of the present utility model, simple in structure, easy to operate; Its test reaction is highly sensitive, sense of smell and taste stimulation can be made a distinction; Therefore use termite olfaction reaction checking device of the present utility model, for judging that food is to tested worm---termite lure character helpful.Termite olfaction reaction checking device of the present utility model also can be common to and detect the olfaction reaction of other walking insect to food.
Embodiment
With reference to above-mentioned accompanying drawing, embodiment of the present utility model is elaborated.Fig. 1, Fig. 2 be in conjunction with having provided a kind of termite olfaction reaction checking device, comprises that one is made up of inside and outside two circles and the container of upper end open, and the inner chamber of inner ring and the inner chamber of outer ring are not connected; Inner ring and outer ring are concentric square, and perhaps inner ring and outer ring are the circle of concentric.When inner ring and outer ring are concentric when square, each edge lengths of inner ring is 7~15cm, and each edge lengths of outer ring is 20~30cm.When inner ring and outer ring were concentric circular, inner ring diameter was 5~15cm, and race diameter is 20~40cm.This inner ring promptly becomes box body 3, this outer ring for bracing frame 4.Placing at the upper end open place of box body 3 can be with the loam cake 1 of box body 3 sealings, and loam cake 1 is provided with air-vent 2, the intracavity inter-connection of this air-vent 2 and box body 3; Air-vent 2 can be over against the positive center of box body 3.This box body 3, bracing frame 4, loam cake 1 are made by clear glass.The height of box body 3 is advisable with 3~5cm.Be provided with 2 closed containers altogether, the bottle 6 of bleeding that each closed container is made by clear glass is formed with the sealing-plug 5 that is positioned at these bottle 6 bottlenecks of bleeding, and sealing-plug 5 plays the effect that seals the bottle 6 of bleeding; It is 250~500ml bottle of bleeding that the bottle 6 of bleeding can be selected capacity for use, and sealing-plug 5 can be made by cotton or silica gel plug.The bottom of being close to box body 3 is provided with the tube connector 9 that 2 clear glasses are made symmetrically with radial, and the radiation initial point is positioned at the positive center of box body 3.The inwall of one end of every tube connector 9 and box body 3 (being inner ring) flushes; The other end of tube connector 9 continues the 1~1.5cm that stretches out after passing bracing frame 4 (being the outer ring), links to each other by flexible pipe 8 the first half with the bottle 6 of bleeding then, and the bracing frame 4 of this moment plays the effect of fixed support tube connector 9.The external diameter of this tube connector 9 is 0.5~0.7cm, and internal diameter should guarantee that termite can pass through smoothly; Equally, the internal diameter of flexible pipe 8 must guarantee that also termite can pass through smoothly.The pedestal 10 that in the bottom of box body 3 and bracing frame 4 height to be set be 8~10cm.
As shown in Figure 3, the course of work that termite olfaction reaction checking device of the present utility model is actual when using is as follows:
Earlier termite 7 is put into the inner chamber of box body 3, cover loam cake 1 then, prevent that termite 7 from fleeing from.Different two kinds of determinands 11,12 (as food or poison bait etc.) are dropped into two bottles 6 of bleeding respectively, and bleeding with 5 pairs of sealing-plugs then bottles 6 seals.Whole detection device is placed in the incubator of lucifuge constant temperature.The smell of determinand 11,12 all enters the inner chamber of box body 3 by volatilizing naturally along the direction of " bottle 6---flexible pipe 8---tube connector 9 of bleeding ".The termite 7 that is arranged in the inner chamber of box body 3 can smell the different smell that determinand 11,12 sends, and then according to the attraction of different smells to it, creeps to the bottle 6 of bleeding that sends respective smell; The distance of creeping is " inner chamber of box body 3---tube connector a 9---flexible pipe 8 ", falls into the bottle 6 of bleeding accordingly then.Because flexible pipe 8 links to each other with the first half of the bottle 6 of bleeding, the termite 7 that therefore falls into the bottle 6 of bleeding can't return the inner chamber of climbing to box body 3 by former distance.After placement after a while, the observer can judge the relative attraction of 11,12 pairs of termites 7 of determinand by the quantity of checking termite 7 in the different bottles 6 of bleeding.Because termite 7 enters all not contacted determinand 11 and determinand 12 before the bottle 6 of bleeding, so determinand 11 and determinand 12 belong to the sense of smell category fully for the what is called " attraction " of termite 7.
Further specify the effect of this checkout gear below in conjunction with three experiments.Experiment condition is as follows: employed termite 7 is eastern subterranean termite Reticulitermes flaviceps in the following experiment, the each test with 10 termites 7.Change other 10 behind the EOT.To determinand 11 and 12, test repeats 10 times for each.For the normal termite 7 of physiological status, under the environmental temperature about 27 ℃, test once lasts 30 minutes to 1 hour.Surpass 1 hour and go to observe, can not exert an influence result of the test.
In example 1, the bottle 6 of bleeding determinand 11-cane powder is housed, in another bottle 6 of bleeding determinand 12-starch is housed.Observe the number of these two termites 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 1.(annotate: numeral is the number of the termite 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistics marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05).
Table 1 termite is to the taxis of cane powder and starch
The 1st group the 2nd group the 3rd group the 4th group the 5th group the 6th group the 7th group the 8th group the 9th group the 10th group average ± SD | 9 10 10 9 9 10 9 10 10 10 9.60±0.52
a | 1 0 0 1 1 0 1 0 0 0 0.40±0.52
b |
In example 2, the bottle 6 of bleeding determinand 11-fermentation cane powder is housed, another is bled and determinand 12-common cane powder is housed in bottles 6.Observe the number of these two termites 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 2.(annotate: numeral is the number of the termite 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistics marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05)
Table 2 termite is to the taxis of fermentation cane powder and common cane powder
| The fermentation cane powder | Common cane powder |
The 1st group the 2nd group the 3rd group the 4th group the 5th group the 6th group the 7th group the 8th group the 9th group | 9 10 10 10 10 10 9 10 10 | 1 0 0 0 0 0 1 0 0 |
The 10th group of average ± SD | 10 9.80±0.42
a | 0 0.20±0.42
b |
In example 3, the bottle 6 of bleeding determinand 11-fermentation cane powder is housed, another is bled and determinand 12-fermentation cane powder+insecticidal active ingredient (40mg/kg) is housed in bottles 6.Observe the number of these two termites 7 in the bottle 6 of bleeding after 1 hour, concrete outcome is as shown in table 3.(annotate: numeral is the number of the termite 7 in the bottle 6 of bleeding that corresponding determinand 11 or 12 are housed in the table; Statistics marks with same letter, shows that difference is not remarkable, on the contrary significant difference, p=0.05)
Table 3 termite is to the taxis of poison bait
| The fermentation cane powder | Fermentation cane powder+insecticidal active ingredient (40mg/kg) |
The 1st group the 2nd group the 3rd group the 4th group the 5th group the 6th group the 7th group the 8th group the 9th group the 10th group average ± SD | 6 6 6 7 6 5 4 4 5 4 5.30±1.06
a | 4 4 4 3 4 5 6 6 5 6 4.70±1.06
a |
Learn from above-mentioned experiment: cane powder has obviously strong sense of smell to attract than starch to termite 7; The fermentation cane powder has obviously strong sense of smell to attract than common unleavened cane powder to termite 7; Add the insecticidal active ingredient of 40mg/kg in the fermentation cane powder made poison bait, its sense of smell attraction to termite 7 does not have significant difference with fermentation cane powder itself.
More than experimental results show that: termite olfaction reaction checking device of the present utility model, test reaction is highly sensitive, can be used to detect the olfaction reaction of insects such as termite to different determinands.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present utility model.Obviously, the utility model is not limited to above embodiment, and many distortion can also be arranged, and for example changes the quantity of bleed bottle 6 and corresponding tube connector 9.All distortion that those of ordinary skill in the art can directly derive or associate from the disclosed content of the utility model all should be thought protection domain of the present utility model.