CN214903201U

CN214903201U - Novel entomopathogenic nematode recruits apparatus

Info

Publication number: CN214903201U
Application number: CN202022459989.6U
Authority: CN
Inventors: 武海斌; 武海波; 刁立功; 范昆; 李美荣; 姜莉莉; 宫庆涛; 王中堂
Original assignee: Shandong Institute of Pomology
Current assignee: Shandong Institute of Pomology
Priority date: 2020-10-29
Filing date: 2020-10-29
Publication date: 2021-11-30
Anticipated expiration: 2030-10-29

Abstract

The utility model relates to a novel entomopathogenic nematode recruits apparatus, including circular transparent year glass board, year glass board center top an organic whole is connected with the fixed bottle of columniform host, and the year glass board of the fixed bottle bottom of host marks out has the circular shape nematode detection zone, carries the glass board edge and upwards extends there is the box dam, and the height that highly is greater than the fixed bottle of host of box dam, the bottleneck department at the fixed bottle top of host is provided with the bottle lid, has all opened screen cloth form aperture on the lateral wall of the fixed bottle of bottle lid and host. The utility model relates to a design concept is ingenious, and simple structure is practical, and the effect is obvious, and the result of use is showing, has good market prospect and popularization and application and worth.

Description

Novel entomopathogenic nematode recruits apparatus

Technical Field

The utility model relates to a novel entomopathogenic nematode recruits apparatus.

Background

Entomopathogenic nematodes (EPNs) are a kind of nematodes which are special parasitic insects, enter a host body by means of invasion of larvae in the 3 rd stage of infection to natural openings or internode membranes of the body of pests, release carried symbiotic bacteria, propagate in the body of the pests, produce toxins and finally cause the death of the pests. Because EPNs have the advantages of wide host range, active host search, safety to non-target organisms, no side effect on the environment, capability of being mixed with a plurality of pesticides and fertilizers and the like, the EPNs are widely applied to control a plurality of agricultural and forestry soil insects such as grubs, cutworms, peach fruit moths, longicorn and the like.

However, the insecticidal effect of entomopathogenic nematodes is slow and unstable compared to chemical insecticides. Research shows that the insecticidal effect is influenced by factors such as biological factors (entomopathogenic nematode strains and dosages, different strains of entomopathogenic nematodes, host insect states and age, and the like), environmental factors (temperature, humidity, insecticide, and the like), application methods (spraying, flood irrigation, and the like), and the like. The difference of the foraging capacity of the nematode strains on the host directly determines the insecticidal effect of the nematode strains. The foraging behavior process of entomopathogenic nematodes can be divided into three main stages of long-distance diffusion, short-distance orientation and final recruitment, and the process of foraging hosts of entomopathogenic nematodes mainly depends on chemical stimulation from the hosts themselves or other substances. The recruitment is the recruitment number of the nematodes within a range of 1-2 mm around the host before the nematodes invade the host, the higher the recruitment number is, the stronger the recruitment capability is, and the strength of the recruitment capability directly determines the key of whether the nematodes can directly invade the host.

Currently, no relevant device or technology exists for researching the recruitment capacity of nematodes. In order to effectively detect the recruitment capacity of the nematode, the recruitment capacity of the nematode is determined. Therefore, a need exists for a novel device and a method for determining the nematode recruitment detection capability of the device, and technical support is provided for nematode application.

SUMMERY OF THE UTILITY MODEL

For solving the technical insufficiency, the utility model provides a simple structure, short test time, the low novel entomopathogenic nematode recruitment apparatus of experimental error.

The utility model discloses a realize through following measure:

the utility model provides a novel entomopathogenic nematode recruits apparatus, includes circular transparent glass-carrying board, carry glass-carrying board central top an organic whole and be connected with the fixed bottle of columniform host, the mark has the circular shape nematode detection zone on the glass-carrying board of the fixed bottle bottom of host, carry glass-carrying board edge upwards to extend there is the box dam, the height that highly is greater than the fixed bottle of host of box dam, the bottleneck department at the fixed bottle top of host is provided with the bottle lid, all open screen cloth form aperture on the lateral wall of bottle lid and the fixed bottle of host.

The glass carrying plate is a circular transparent plastic plate, the diameter of the glass carrying plate is 3-12 cm, the thickness of the glass carrying plate is 1-2 mm, the height of the box dam is 1-3 cm, and the thickness of the box dam is consistent with that of the glass carrying plate.

The nematode detection zone is circular and is located the center of carrying the glass board, and the diameter is 2~6cm, and the nematode detection zone is provided with black bottom, is painted with the gridlines on the bottom, and the square that the gridlines cross formation, its length of side is 1mm ~2mm, and the gridlines colour can be red, green or blue.

The diameter of the bottom of the host fixing bottle is smaller than that of the nematode detection area and is 1-5 cm, the thickness is 0.5mm, and the height is 0.5-2.5 cm.

The utility model has the advantages that: the utility model discloses divide into different model structures, can use different nematode strains to same host insect, also can use same nematode strain to different host insects, still can use the research of natural open-ended recruitment ability such as same line strain to same host insect mouth, anus and gas pocket. 2. The utility model discloses an overall structure, convenient and fast during the use is convenient for develop different experimental research. 3. The utility model discloses the bulk material is transparent plastic. The purpose is to facilitate carrying and transportation. 4. The utility model discloses can be according to host insect's size (like the small-size insects such as leek maggot, bactrocera dorsalis larva or peach fruit borer, walnut are lifted medium-sized insects such as limb moth or large-scale insects such as little cutworm, grub, wax moth), the nematode recruitment apparatus of different models of selectable has reduced experimental error. 5. The utility model relates to a design concept is ingenious, and simple structure is practical, and the effect is obvious, and the result of use is showing, has good market prospect and popularization and application and worth.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

FIG. 2 is a schematic view of the structure of a host fixing bottle.

FIG. 3 is a schematic view of the whole apparatus for detecting the recruitment of entomopathogenic nematodes.

Wherein, 1, glass carrying plate; 2. a box dam; 3. a nematode detection zone; 4. grid lines; 5. entomopathogenic nematodes; 6. a host fixing bottle; 7. a bottle cap; 8. a small hole; 10. A host insect.

Detailed Description

The invention is described in further detail below with reference to the accompanying drawings:

as shown in fig. 1, 2 and 3, a novel entomopathogenic nematode recruitment determinator comprises a round transparent glass carrying plate 1, wherein a cylindrical host fixing bottle 6 is integrally connected above the center of the glass carrying plate 1, a round nematode detection area 3 is marked on the glass carrying plate 1 at the bottom of the host fixing bottle 6, a dam 2 extends upwards from the edge of the glass carrying plate 1, the height of the dam 2 is greater than that of the host fixing bottle 6, a bottle cap 7 is arranged at the bottle mouth at the top of the host fixing bottle 6, and screen-shaped small holes 8 are formed in the side walls of the bottle cap 7 and the host fixing bottle 6.

The glass carrying plate is a circular transparent plastic plate, the diameter of the glass carrying plate is 3-12 cm, the thickness of the glass carrying plate is 1-2 mm, the height of the box dam 2 is 1-3 cm, and the thickness of the box dam 2 is consistent with that of the glass carrying plate 1. The nematode detection zone 3 is circular and is located the center of carrying the glass board, and the diameter is 2~6cm, and nematode detection zone 3 is provided with black bottom, is painted grid line 4 on the bottom, and the square that grid line 4 alternately formed, its length of side is 1mm ~2mm, and the 4 colours of grid line can be red, green or blue. The diameter of the bottom of the host fixing bottle 6 is smaller than that of the nematode detection area 3 and is 1-5 cm, the thickness is 0.5mm, and the height is 0.5-2.5 cm.

The glass carrying plate 1 is made of circular polyethylene, polypropylene, polymethacrylate, polyethylene terephthalate, polycarbonate resin, polystyrene and other types of plastic materials, and has the advantage that the black grid lines 4 on the nematode detection area 3 marked on the glass carrying plate 1 can be seen clearly under a microscope. The diameter of the circular glass carrying plate 1 is 3-12 cm, the thickness is 1-2 mm, and the design can meet the research on the recruitment capacity of entomopathogenic nematodes 5 to host insects 10 of different sizes. The height of the box dam 2 is 1-3 cm, and the thickness of the box dam is consistent with the material and thickness of the glass carrying plate 1. The bottom of the dam 2 and the front surface of the glass carrying plate 1 are of an integral structure, and when the recruitment capacity of the entomopathogenic nematodes 5 is detected, the entomopathogenic nematodes 5 are prevented from moving out of the glass carrying plate 1. The nematode detection zone 3 is located the central point of year glass board, and the diameter is 2~6cm, and the setting of nematode detection zone 3 bottom plate is black, is painted grid line 4 on the bottom plate, and the square that grid line 4 alternately formed, its length of side is 1mm ~2mm, and grid line 4 colour can be red, one of green and blue, and the purpose of this design is because entomopathogenic nematode 5 is the milk white, and the entomopathogenic nematode 5 of being convenient for under this colour observes and counts under the microscope. The fixed bottle of host 6 is cylindrical, and the bottom is located the central part in nematode detection zone 3, and the whole colour of the fixed bottle of host 6 is black, and the circular diameter in bottom is 1~5cm, is less than the diameter in nematode detection zone 3, and the purpose of this design is difficult to observe under the microscope when preventing host insect 10 to hug closely at the fixed bottle wall of host 6. The thickness of the host fixing bottle 6 is 0.5mm, the height of the host fixing bottle is 0.5-2.5 cm lower than that of the dam 2, and the host insect 10 can be completely immersed in the pluronic gel through the design, so that observation of nematodes is facilitated. The periphery of the bottom of the host fixing bottle 6 and the nematode detection area 3 are of an integral structure. The host fixing bottle 6 is provided with a bottle cap 7, and the thickness and the diameter of the bottle cap 7 are consistent with those of the host fixing bottle 6. The bottle cap 7 and the side wall of the host fixing bottle 6 are both provided with the screen-shaped small holes 8, and the diameter of the screen-shaped small holes 8 is 3-5 mm, so that the host fixing bottle is favorable for free movement of nematodes, and can prevent the host insects 10 from leaving the nematode detection area 3, thereby facilitating detection of the nematodes. The host insect 10 may be small insects such as chive maggot, bactrocera dorsalis larva, peach fruit borer, etc., or large insects such as black cutworm, grub, wax moth, etc. The recruitment device of the present invention is capable of detecting nematode concentrations within the normal use range.

Through the utility model discloses a when entomopathogenic nematode 5 recruits ability detection device, through putting 1 host insect 10 in the fixed bottle 6 of host earlier to cover the bottle lid 7 of the fixed bottle 6 of host. mu.L of entomopathogenic nematode 5 suspension (about 400 strips) was gently stirred with an amount of pluronic gel in a beaker until well mixed. The mixed solution of the prepared pluronic gel and the entomopathogenic nematodes 5 is added into the circular glass carrying plate until the thickness of the mixed solution exceeds the height of the host fixing bottle 6 and the mixed solution does not overflow. After various times of treatment, the number of entomopathogenic nematodes 5 in the grid surrounding the host insect 10 in the nematode detection zone 3 was observed under a stereomicroscope. When the number of the nematodes is investigated, when the nematode polypide is on the cross line of the grid line 4, the polypide size is more than or equal to half of the size in the grid line 4, and the nematode is 1; when less than half, it is ignored. The recruitment capacity of entomopathogenic nematodes 55 is evaluated by calculating the attraction rate of the entomopathogenic nematodes moving into the grid surrounding the host insect 1010

The utility model discloses a device can use and can use different nematode strains to same host insect 10, also can use same nematode strain to different host insects 10, still can use the research of natural open-ended recruitment ability such as same line strain to same host insect 10 mouths, anus and gas pocket. Suitable for all nematode strains currently recorded or not recorded, including all nematode strains used in the genera Steelegans and Heterodera.

The foregoing is only a preferred embodiment of this patent, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of this patent, and these modifications and substitutions should also be regarded as the protection scope of this patent.

Claims

1. A novel entomopathogenic nematode recruitment tester is characterized in that: including circular transparent year glass board, carry glass board center top an organic whole and be connected with the fixed bottle of columniform host, the year glass board of the fixed bottle bottom of host marks out has the circular shape nematode detection zone, it upwards extends to carry the glass board edge has the box dam, the height that highly is greater than the fixed bottle of host of box dam, the bottleneck department at the fixed bottle top of host is provided with the bottle lid, all open screen cloth form aperture on the lateral wall of the fixed bottle of bottle lid and host.

2. The novel entomopathogenic nematode recruitment assay of claim 1, wherein: the glass carrying plate is a circular transparent plastic plate, the diameter of the glass carrying plate is 3-12 cm, the thickness of the glass carrying plate is 1-2 mm, the height of the box dam is 1-3 cm, and the thickness of the box dam is consistent with that of the glass carrying plate.

3. The novel entomopathogenic nematode recruitment assay of claim 1, wherein: the nematode detection zone is circular and is located the center of carrying the glass board, and the diameter is 2~6cm, and the nematode detection zone is provided with black bottom, is painted with the gridlines on the bottom, and the square that the gridlines cross formation, its length of side is 1mm ~2mm, and the gridlines colour can be red, green or blue.

4. The novel entomopathogenic nematode recruitment assay of claim 1, wherein: the diameter of the bottom of the host fixing bottle is smaller than that of the nematode detection area and is 1-5 cm, the thickness is 0.5mm, and the height is 0.5-2.5 cm.