CN2489340Y - Intelligence magnetic separation enzyme-linked immuno-tester - Google Patents

Abstract

The utility model relates to an intelligence magnetic separation enzyme-linked immuno-tester. A light path system comprises a bromine tungsten-arc lamp which can be suitable to wavelength of 492nm, 550nm and 630nm, a two-stage light splitter, a light filter which can be suitable to narrowband of 492nm, 550nm and 630nm and a light detector. The light sent from the light source is divided by a first grade light splitter into two beams after passing through the detected object. One beam is sent to the light detector by passing through the light filter with a narrowband of 630nm, while the other beam is divided by a second grade light splitter into two beams which are respectively sent to the light detector through the light filter with a narrowband of 550nm and a narrowband of 492nm. The signal detected by the light detector is sent into a chip selecting circuit after being amplified by the amplifier, and then the signal is sent into a computer through an A/D circuit and an I/O interface circuit.

Description

The separation enzyme-linked immunoassay instrument of intelligent magnetic

The utility model belongs to the optics biochemical instrument, relates to a kind of magnetic enzyme linked immunological tester, the separation enzyme-linked immunoassay instrument of particularly a kind of intelligent magnetic.

Human body trace and trace materials are measured and are mainly used the enzyme immunization method to detect at present, in enzyme immunoassay method, round as the label of measuring media, have occurred some kinds of measuring methods again.Common have EIA enzyme immunoassay, enzyme immunosorbent assay, the separation enzyme-linked immunoassays of magnetic, fluorescence immunoassay, rapid fluorescence enzyme mark and luminescent immunoassay or the like.The separation enzyme-linked immunoassays of magnetic wherein are advanced technologies of a kind of heterotope immune detection of Switzerland's invention in the latter stage eighties, also are called magnetic antibody mediated immunity technology.This technology is immuno-enzymatic system (magnetic-particle) combination, uses a kind of determination method of two kinds of high-affinity monoclonal antibodies, and it makes the double-antibody sandwich Enzyme-multiplied immune technique is being to separate fully apace in the antibody complex free property labelled antibody with antigen.Outstanding advantages such as it has hypersensitivity, high specific, high stability, pollution-free, avirulence, the reagent storage frame life-span is long, linear measurement range is wide, detections is rapid, aspect microdetermination and the radiommunoassay of typing reach same level.In the separation enzyme-linked method of immunity of magnetic, what adopted is three light wave photometers of a discontinuous wavelength, is used for measuring solution absorbency in the test tube.Establish microprocessor in the instrument, it can become concentration value with the absorbance numerical transformation that reads.But this instrument can not cooperate closely with computing machine, does not also possess data management function, and this trend with the computing machine high speed development does not conform to very much, hospital's implementation computer management also is one regrets.This instrument also has a fatal shortcoming, because may there be error in the surface topography of test tube, and factors such as scratch, impression of the hand, booty, surperficial little foam, liquid fouling product all can be brought error to measurement result exactly when measuring.

The purpose of this utility model is to overcome the deficiencies in the prior art, the separation enzyme-linked immunoassay instrument of magnetic is combined with computing machine, allow computing machine participate in checked operation and data are handled, make it have certain intelligentized detection control and statistical study ability, and eliminate the error of bringing because of foreign material.

The utility model is achieved through the following technical solutions: the separation enzyme-linked immunoassay instrument of a kind of intelligent magnetic, comprise light path system, simulation amplifier section, part of data acquisition, computing machine and printer, it is characterized in that: described light path system comprises bromine tungsten filament lamp, first order optical splitter, second level optical splitter, 492nm, 550nm, the 630nm narrow band pass filter that can be applicable to 492nm, 50nm, 630nm wavelength and can accept three road light signals of different wave length respectively and convert thereof into the photodetector of electric signal; Part of data acquisition comprises data acquisition chip select circuit, A/D change-over circuit and I/O interface circuit; The light that sends from light source is divided into two bundles through behind the measured object by first order optical splitter, a branch of through the 630nm narrow band pass filter to photodetector, another Shu Guangzai through second level optical splitter be divided into two bundles respectively through 550nm and 492nm narrow band pass filter to photodetector, the signal that photodetector detects is amplified into chip select circuit through amplifier, enters computing machine through A/D circuit and I/O interface circuit again.

The utility model combines with general calculation machine hardware and software, utilizing enzyme mark final reaction product phenolphthalein is 0 to 630nm wavelength absorbance, to 550nm wavelength absorbance maximum, to 492nm wavelength absorbance is 1/5 characteristic of 550nm wavelength absorbance, introduced the mensuration of 630nm wavelength, the measurement result of promptly representing blank test tube with the measured value of 630nm wavelength, processing by computing machine, only need 550nm, the absorbance of 492nm deducts the absorbance of 630nm, equal in spectrum test, to have deducted the influence of foreign material, thereby eliminated the error that causes because of foreign material.Using a computer has replaced original microprocessor, has improved computing velocity, has possessed storage space and data exchange interface.Thereby the statistical report form and the inspection sheet that can enable printer print all kinds of standard formats also can be eliminated the error of bringing because of system fluctuation with repeatedly calculating the way of averaging; Carrying out intelligentized processing aspect the ordering that detects sample, solving mark this shop and test tube measuring sequence and intersected the problem of check mark.

The utility model is described in further detail below in conjunction with embodiment of the present utility model:

Description of drawings:

Fig. 1～the utility model hardware block diagram

Fig. 2～the utility model structural front view

Fig. 3～the utility model structure vertical view

Fig. 4～the utility model structure left view

An embodiment of the present utility model has been showed in Fig. 1～4, the light transmission measured object that light source sends is after first order optical splitter is divided into two bundles, a branch of through the 630nm narrow band pass filter to photodetector, another Shu Zaijing second level optical splitter is divided into two bundles, respectively behind 550nm and 492nm narrow band pass filter to photodetector.630nm, 550nm and 492nm narrow band pass filter can be isolated the light wave of three kinds of wavelength, convert electric signal respectively to through photodetector, be amplified into chip select circuit selecting and purchasing respectively respectively through amplifier, enter computing machine through A/D conversion, I/O interface respectively again, connect display and printer.The utility model has been selected 586 computing machines and common printer for use.In fact, the utility model is with floppy drive 2, hard disk 3, mainboard 4, power supply 5, light source 8, transformer 9, light base module 10, all place in the same casing 1, upper surface at casing 1 is provided with audio amplifier 7, pilot lamp 11 (Huang), 12 (green), keyboard 13, is provided with leg 6 below casing 1.Optical splitter, optical filter, photodetector, amplifier, chip select circuit, A/D change-over circuit, I/O interface circuit all are contained on the light base module 10.

The utility model is made according to the reaction principle of the separation enzyme-linked immunoreagent of magnetic.Adopted alkaline phosphatase to mark as enzyme, its final reaction product is a phenolphthalein, and when enzyme linked immunoassay finished, it is pink that liquid shows.By spectral analysis as can be known, on the 550nm wavelength, to the phenolphthalein color survey the absorbance maximum, absorbance is 0 (minimum value) on the 630nm wavelength, and when absorbance is the 552nm wavelength just on the 492nm wavelength 1/5 of measured value.The utility model has utilized this relation of 550nm wavelength and 492nm wavelength dexterously, with chip select circuit transfer point is controlled, the color is too dark causes instrument to occur automatically switching to the 492nm section when saturated at the signal of 550nm measuring section to measure when phenolphthalein, make the linear measurement range of instrument expand to five times of common survey scope, avoided when measuring because of the excessive process that must dilute of sample concentration.

The utility model uses a computer and has replaced original microprocessor, improved test speed, also possessed data space and data exchange interface, can utilize the repertoire of computing machine to realize printing, networking data transmission or the like, increase maintenance, inquiry, statistics and management function various data.Because being designed to three light paths in analog amplify circuit amplifies respectively, three road signals is chosen respectively carried out analog to digital conversion again, can change into monochromatic light road colorimetric estimation system from three road colorimetric systems easily, makes instrument possess certain extended capability.

Claims (3)

1, the separation enzyme-linked immunoassay instrument of a kind of intelligent magnetic, comprise light path system, simulation amplifier section, part of data acquisition, computing machine and printer, it is characterized in that: described light path system comprises bromine tungsten filament lamp, first order optical splitter, second level optical splitter, 492nm, 550nm, the 630nm narrow band pass filter that can be applicable to 492nm, 550nm, 630nm wavelength and can accept three road light signals of different wave length respectively and convert thereof into the photodetector of electric signal; Part of data acquisition comprises data acquisition chip select circuit, A/D change-over circuit and I/O interface circuit; The light that sends from light source is divided into two bundles through behind the measured object by first order optical splitter, a branch of through the 630nm narrow band pass filter to photodetector, another Shu Guangzai through second level optical splitter be divided into two bundles respectively through 550nm and 492nm narrow band pass filter to photodetector, the signal that photodetector detects is amplified into chip select circuit through amplifier, enters computing machine through A/D circuit and I/O interface circuit again.

2, according to the separation enzyme-linked immunoassay instrument of the described intelligent magnetic of claim 1, it is characterized in that 630nm, 550nm and 492nm narrow band pass filter isolate the light wave of three kinds of wavelength, convert electric signal respectively to through photodetector, be amplified into chip select circuit selecting and purchasing respectively respectively through amplifier, enter computing machine through A/D conversion, I/O interface respectively again.

3, according to claim 1 or the separation enzyme-linked immunoassay instrument of 2 described intelligent magnetic, it is characterized in that described optical splitter, optical filter, photodetector, amplifier, chip select circuit, A/D change-over circuit, I/O interface circuit all are contained on the light base module (10), floppy drive (2), hard disk (3), mainboard (4), power supply (5), light source (8), transformer (9), light base module (10), all place in the same casing (1).

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