CN205246666U - Fluorescence immunity chromatography quantitative analysis instrument - Google Patents
Fluorescence immunity chromatography quantitative analysis instrument Download PDFInfo
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- CN205246666U CN205246666U CN201520848421.XU CN201520848421U CN205246666U CN 205246666 U CN205246666 U CN 205246666U CN 201520848421 U CN201520848421 U CN 201520848421U CN 205246666 U CN205246666 U CN 205246666U
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- determinand
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Abstract
The utility model discloses a fluorescence immunity chromatography quantitative analysis instrument, including controller, processing circuit and fluorescence immunity optical detection module, fluorescence immunity optical detection module includes the light source, a plano -convex lens, silicon photocell, the dichroic mirror, the 2nd plano -convex lens, aperture diaphragm and test paper strip, the controller is connected with the control end of light source, on the determinand light that the light source sent shines the test paper strip again behind a plano -convex lens after the dichroic mirror reflection in, the light that shines on the determinand divide into two parts, wherein, some is absorbed by the determinand, another part reflects through the determinand, a plano -convex lens, the dichroic mirror, shine behind the 2nd plano -convex lens and the aperture diaphragm on silicon photocell's the photosensitive surface, silicon photocell's signal output part is connected with processing circuit's input, processing circuit's output is connected with the input of controller. The utility model discloses can realize fluorescence immunity chromatography quantitative determination to cost reduction.
Description
Technical field
The utility model belongs to quarantine detection technique field, relates to a kind of fluorescence immune chromatography and quantitatively dividesAnalyse instrument.
Background technology
Fluorescence immune chromatography technology is easy and simple to handle, quick because of it, and has very high specificity and spiritQuick property, must will replace colloidal gold immunochromatographimethod technology, and be generalized to clenobuterol hydrochloride, RecDopamine, salbutamol, melamine, chloramphenicol, tetracycline furans antibiotic, sulfamidoDeng residue of veterinary drug, Escherichia coli, aftosa, blue otopathy, avian influenza virus, NDV etc.The food security inspection of the toxin such as animal epidemic and zearalenone, aflatoxin, vomitoxinSurvey field. Learn according to existing market investigation, the Yangtze River Delta and Pearl River Delta one with former colloidal gold stripManufacturer, drops into one after another technical force and is ready to complete from collaurum qualitative analysis and divides to fluorescent quantitationThe transition of analysing. Independent research has been inspired confidence in for example Shenzhen ten thousand " flying to survey " serial detector, supporting fluorescence inspectionTest paper slip, detects the indexs such as cardiac troponin, be received by the market very much, but precision is slightly poor; SeparatelyThe supporting external high end instrument of outer producers carries out test strips sale, high cost, the market competitivenessLow; Also have more test strips manufacturer, being confined to does not have suitable quantitative fluorescence analysis instrument,Slowly cannot release product, incur loss through delay valuable opportunity. Therefore, if can develop a set of high performanceFluorescence immune chromatography quantitative analysis instrument, will greatly effectively promote the development of relevant industries, Er QiejuEarly stage, market was estimated, and was only specialized market, and the demand of annual instrument should be more than 50,000,Market valuation is more than 600,000,000 yuans.
Utility model content
The purpose of this utility model is to overcome the shortcoming of above-mentioned prior art, and a kind of fluorescence is providedImmunochromatography quantitative analysis instrument, this instrument can be realized fluorescence immune chromatography and quantitatively detect, andCost.
For achieving the above object, fluorescence immune chromatography quantitative analysis instrument bag described in the utility modelDraw together controller, signal processing circuit and fluorescence immunoassay optical detecting module;
Fluorescence immunoassay optical detecting module comprises light source, the first planoconvex spotlight, silicon photocell, two looksMirror, the second planoconvex spotlight, aperture and test strips, controller is connected with the control end of light source,The light that light source sends is irradiated to treating in test strips after dichroic mirror reflection after the first planoconvex spotlight againSurvey on thing, the light being irradiated on determinand is divided into two parts, and wherein, a part is absorbed by determinand,Another part is through determinand reflection, the first planoconvex spotlight, dichroic mirror, the second planoconvex spotlight and apertureAfter diaphragm, be irradiated on the photosurface of silicon photocell the signal output part of silicon photocell and signal processingThe input of circuit is connected, and the output of signal processing circuit is connected with the input of controller.
The light that light source sends incides on dichroic mirror through the first optical filter.
Between dichroic mirror and the second planoconvex spotlight, be provided with the second optical filter.
Also comprise stepper motor, slide rail, screw mandrel and slide unit, fluorescence immunoassay optical detecting module is positioned atOn described slide unit, the output shaft of stepper motor is connected with one end of screw mandrel, the other end of screw mandrel withSlide unit is threaded connection, and slide unit is positioned on described slide rail.
Described signal processing circuit comprises I/V change-over circuit, filter circuit, synchronous demodulator and ADConversion chip, the signal output part of silicon photocell is connected with the input of I/V change-over circuit, I/VThe output of change-over circuit is successively through filter circuit, synchronous demodulator and AD conversion chip and controlThe input of device is connected.
Described I/V change-over circuit comprises ultra-low noise operational amplifier.
Described controller is also connected with display, memory and light source parameters input module.
The utlity model has following beneficial effect:
Fluorescence immune chromatography quantitative analysis instrument described in the utility model, in testing process, is controlledThe light that device control light source sends absorbs a part through determinand, and another part light is changed through silicon photocellFor current signal, described current signal is converted to voltage signal after signal processing circuit is processed, controlThe light intensity of the light that device processed must detect according to described voltage signal, then the light sending according to light source is strongSpend the concentration that obtains determinand with the difference of the light intensity detecting, realize fluorescence immune chromatography quantitativeDetect, cost is lower.
Further, fluorescence immunoassay optical detecting module is positioned on described slide unit, the output of stepper motorAxle is connected with one end of screw mandrel, and the other end and the slide unit of screw mandrel are threaded connection, and slide unit is positioned atOn described slide rail, thereby can realize, test strips entirety is detected, the scope of detection is wider.
Brief description of the drawings
Fig. 1 is the schematic diagram of fluorescence immunoassay optical detecting module in the utility model.
Wherein, 1 for light source, 2 be the first optical filter, 3 for dichroic mirror, 4 be the first planoconvex spotlight,5 is that test strips, 6 is that the second optical filter, 7 is that the second planoconvex spotlight, 8 is for aperture, 9Silicon photocell.
Detailed description of the invention
Below in conjunction with accompanying drawing, the utility model is described in further detail:
With reference to figure 1, fluorescence immune chromatography quantitative analysis instrument described in the utility model comprises controlDevice, signal processing circuit and fluorescence immunoassay optical detecting module; Fluorescence immunoassay optical detecting module bagDraw together light source 1, the first planoconvex spotlight 4, silicon photocell 9, dichroic mirror 3, the second planoconvex spotlight 7,Aperture 8 and test strips 5, controller is connected with the control end of light source 1, and light source 1 sendsLight after dichroic mirror 3 reflection, after the first planoconvex spotlight 4, be irradiated to again to be measured in test strips 5On thing, the light being irradiated on determinand is divided into two parts, and wherein, a part is absorbed by determinand,Another part is through determinand reflection, the first planoconvex spotlight 4, dichroic mirror 3, the second planoconvex spotlight 7And after aperture 8, be irradiated on the photosurface of silicon photocell 9 the signal output of silicon photocell 9End is connected with the input of signal processing circuit, the output of signal processing circuit and controllerInput is connected.
It should be noted that, the light that light source 1 sends incides on dichroic mirror 3 through the first optical filter 2,Between dichroic mirror 3 and the second planoconvex spotlight 7, be provided with the second optical filter 6.
The utility model also comprises stepper motor, slide rail, screw mandrel and slide unit, the inspection of fluorescence immunoassay opticsSurvey module and be positioned on described slide unit, the output shaft of stepper motor is connected with one end of screw mandrel, screw mandrelThe other end and slide unit be threaded connection, slide unit is positioned on described slide rail, signal processing circuit bagDraw together I/V change-over circuit, filter circuit, synchronous demodulator and AD conversion chip, silicon photocell 9Signal output part be connected with the input of I/V change-over circuit, the output of I/V change-over circuit is complied withInferiorly be connected with the input of controller through filter circuit, synchronous demodulator and AD conversion chip,I/V change-over circuit comprises ultra-low noise operational amplifier, and controller is also connected with display, storageDevice and light source parameters input module.
Specific operation process of the present utility model is:
Controller is luminous according to the intensity signal control light source 1 of user's input, the light that light source 1 sendsAfter dichroic mirror 3 refractions, be irradiated on the determinand in test strips 5 through the first planoconvex spotlight 4 again,The light being irradiated on determinand is divided into two parts, and wherein, branch's light is absorbed by determinand, anotherPart light is irradiated on dichroic mirror 3, then after determinand reflection after the first planoconvex spotlight 4 againAfter dichroic mirror 3 transmissions, after the second planoconvex spotlight 7 and aperture 8, be irradiated to silicon photocell againOn 9 photosurface, make silicon photocell 9 generation current signals, the electric current letter that silicon photocell 9 producesNumber be converted to voltage signal and described voltage signal is amplified through I/V change-over circuit, after amplificationVoltage signal successively through filter circuit filtering, synchronous demodulator synchronous demodulation and AD conversion coreAfter sheet AD conversion, be input in controller, controller is according to the magnitude of voltage of the voltage signal receivingThe light intensity value of the light that must detect, and then according to the intensity signal of user's input and the light detectingThe difference of light intensity value obtain the concentration of determinand.
Described light source 1 is LED lamp, and described silicon photocell 9 is photodiode, and described AD turnsChanging chip is 24 sigma-delta type AD conversion chips of low noise.
The utility model overcome conventional time-resolved fluorescence detect the fluorescence signal that principle exists weak,Be subject to the technological difficulties of ambient light interference, improved system detection sensitivity, detect in guarantee systemUnder the prerequisite of sensitivity, also provide the experimental prototype development plan of a kind of low cost, high performance-price ratio,Can directly meet market competition in the urgent need to. Scan in addition clear area, survey in test strips 5Amount band and quality control band spectral line, clear area adopts smothing filtering Denoising Algorithm, and measures band, quality control bandAdopt " curve of spectrum integration " algorithm, intelligent quantitative analysis determinand sample concentration, detects sensitiveDegree is more than 1%. Therefore the utility model can be applied to food security field, can detect multipleMaterial, comprises clenbuterol hydrochloride, melamine, chloramphenicol, tetracycline furans antibiotic, sulfamidoDeng residue of veterinary drug, Escherichia coli, aftosa, blue otopathy, avian influenza virus, NDV etc.The toxin such as animal epidemic and zearalenone, aflatoxin, vomitoxin.
Claims (7)
1. a fluorescence immune chromatography quantitative analysis instrument, is characterized in that, comprises controller, signal processing circuit and fluorescence immunoassay optical detecting module;
Fluorescence immunoassay optical detecting module comprises light source (1), the first planoconvex spotlight (4), silicon photocell (9), dichroic mirror (3), the second planoconvex spotlight (7), aperture (8) and test strips (5), controller is connected with the control end of light source (1), the light that light source (1) sends is irradiated on the determinand in test strips (5) after dichroic mirror (3) reflection after the first planoconvex spotlight (4) again, the light being irradiated on determinand is divided into two parts, wherein, a part is absorbed by determinand, another part reflects through determinand, the first planoconvex spotlight (4), dichroic mirror (3), after the second planoconvex spotlight (7) and aperture (8), be irradiated on the photosurface of silicon photocell (9), the signal output part of silicon photocell (9) is connected with the input of signal processing circuit, the output of signal processing circuit is connected with the input of controller.
2. fluorescence immune chromatography quantitative analysis instrument according to claim 1, is characterized in that, the light that light source (1) sends incides on dichroic mirror (3) through the first optical filter (2).
3. fluorescence immune chromatography quantitative analysis instrument according to claim 2, is characterized in that, between dichroic mirror (3) and the second planoconvex spotlight (7), is provided with the second optical filter (6).
4. fluorescence immune chromatography quantitative analysis instrument according to claim 1, it is characterized in that, also comprise stepper motor, slide rail, screw mandrel and slide unit, fluorescence immunoassay optical detecting module is positioned on described slide unit, the output shaft of stepper motor is connected with one end of screw mandrel, the other end and the slide unit of screw mandrel are threaded connection, and slide unit is positioned on described slide rail.
5. fluorescence immune chromatography quantitative analysis instrument according to claim 1, it is characterized in that, described signal processing circuit comprises I/V change-over circuit, filter circuit, synchronous demodulator and AD conversion chip, the signal output part of silicon photocell (9) is connected with the input of I/V change-over circuit, and the output of I/V change-over circuit is connected with the input of controller through filter circuit, synchronous demodulator and AD conversion chip successively.
6. fluorescence immune chromatography quantitative analysis instrument according to claim 5, is characterized in that, described I/V change-over circuit comprises ultra-low noise operational amplifier.
7. fluorescence immune chromatography quantitative analysis instrument according to claim 1, is characterized in that, described controller is also connected with display, memory and light source parameters input module.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520848421.XU CN205246666U (en) | 2015-10-29 | 2015-10-29 | Fluorescence immunity chromatography quantitative analysis instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520848421.XU CN205246666U (en) | 2015-10-29 | 2015-10-29 | Fluorescence immunity chromatography quantitative analysis instrument |
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| CN205246666U true CN205246666U (en) | 2016-05-18 |
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| CN201520848421.XU Expired - Fee Related CN205246666U (en) | 2015-10-29 | 2015-10-29 | Fluorescence immunity chromatography quantitative analysis instrument |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242039A (en) * | 2015-10-29 | 2016-01-13 | 詹爱军 | Fluorescent immunochromatography quantitative analyzer and method |
| WO2018058525A1 (en) * | 2016-09-30 | 2018-04-05 | 深圳市检验检疫科学研究院 | Fluorescence immunochromatography quantitative analysis instrument and method |
| CN108414484A (en) * | 2018-03-02 | 2018-08-17 | 江西英大生物技术有限公司 | Fully-automatic dry type fluorescence immunity analyzer |
| CN108535471A (en) * | 2017-03-06 | 2018-09-14 | 广州万孚生物技术股份有限公司 | Immunofluorescence test light path mechanism, Immunofluorescence test instrument and its calibration method |
| CN112345502A (en) * | 2020-10-27 | 2021-02-09 | 天津市英贝特航天科技有限公司 | Detector for eliminating fluorescence noise in fluorescence immunoassay analyzer |
-
2015
- 2015-10-29 CN CN201520848421.XU patent/CN205246666U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105242039A (en) * | 2015-10-29 | 2016-01-13 | 詹爱军 | Fluorescent immunochromatography quantitative analyzer and method |
| WO2018058525A1 (en) * | 2016-09-30 | 2018-04-05 | 深圳市检验检疫科学研究院 | Fluorescence immunochromatography quantitative analysis instrument and method |
| CN108535471A (en) * | 2017-03-06 | 2018-09-14 | 广州万孚生物技术股份有限公司 | Immunofluorescence test light path mechanism, Immunofluorescence test instrument and its calibration method |
| CN108414484A (en) * | 2018-03-02 | 2018-08-17 | 江西英大生物技术有限公司 | Fully-automatic dry type fluorescence immunity analyzer |
| CN112345502A (en) * | 2020-10-27 | 2021-02-09 | 天津市英贝特航天科技有限公司 | Detector for eliminating fluorescence noise in fluorescence immunoassay analyzer |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160518 Termination date: 20201029 |