CN221071504U - Biological bacterial liquid fermentation reaction tank capable of estimating bacterial colony number - Google Patents
Biological bacterial liquid fermentation reaction tank capable of estimating bacterial colony number Download PDFInfo
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- CN221071504U CN221071504U CN202322451134.2U CN202322451134U CN221071504U CN 221071504 U CN221071504 U CN 221071504U CN 202322451134 U CN202322451134 U CN 202322451134U CN 221071504 U CN221071504 U CN 221071504U
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- reaction tank
- biological
- estimating
- culture medium
- dilution
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 58
- 239000007788 liquid Substances 0.000 title claims abstract description 41
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 39
- 238000000855 fermentation Methods 0.000 title claims abstract description 28
- 230000004151 fermentation Effects 0.000 title claims abstract description 28
- 238000010790 dilution Methods 0.000 claims abstract description 24
- 239000012895 dilution Substances 0.000 claims abstract description 24
- 239000001963 growth medium Substances 0.000 claims abstract description 21
- 238000005070 sampling Methods 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 241000233866 Fungi Species 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000013060 biological fluid Substances 0.000 claims 6
- 210000001503 joint Anatomy 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 abstract description 8
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 238000007789 sealing Methods 0.000 description 7
- 244000005700 microbiome Species 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
Abstract
The utility model discloses a biological bacterial liquid fermentation reaction tank capable of estimating the number of bacterial colonies, which belongs to the field of fermentation reaction tanks, and aims to solve the problems that the biological bacterial liquid is sampled at fixed time, the reaction tank is opened for each sampling, the exposure of the microbial environment and the risk of external pollution can be caused, and the possibility of microbial pollution still exists even if strict aseptic operation is adopted; according to the utility model, the dilution liquid is introduced through the dilution conduit, falls onto the biological bacterial liquid along the slotted hole of the funnel, after the culture is carried out for a period of time, the number of bacterial colonies growing on the culture medium is observed, and estimation of the number of bacterial colonies can help to determine whether the supply of nutrient elements in the culture medium is enough or not, and which components need to be regulated or supplemented, so that the biological bacterial liquid reaction in the reaction tank is facilitated to be promoted, and the fermentation process is regulated to achieve the optimal product yield.
Description
Technical Field
The utility model relates to the field of fermentation reaction tanks, in particular to a biological bacterial liquid fermentation reaction tank capable of estimating the number of bacterial colonies.
Background
The reaction tank is usually composed of a plurality of components including a reaction vessel, a stirring system, an air intake and exhaust system, a temperature control system, a pH control system, a circulation cooling system, a sampling port, a monitoring and control device, etc., during fermentation, microbial bacteria liquid is placed in the reaction vessel, the stirring system thoroughly mixes the fermentation liquid by stirring paddles or stirrers to provide sufficient oxygen and nutrients for the growth of microorganisms, the air intake and exhaust system controls the flow of air in the reaction tank to ensure proper oxygen and carbon dioxide content, the temperature control system can adjust and maintain a constant temperature of the fermentation liquid to accommodate the growth requirements of microorganisms, the pH control system is used to monitor and adjust the acid-base balance of the reaction liquid to provide a proper pH environment, some fermentation processes require low temperature conditions, and thus may be equipped with a circulation cooling system to maintain a constant temperature of the fermentation liquid, however, the colony count is an important index for evaluating fermentation effect and activity, in order to understand the growth rate of microorganisms at different time points, metabolic activity and fermentation efficiency, sampling the biological bacteria liquid needs to be timed, which involves opening the reaction tank, which may lead to exposure to microbial environment and exposure and external contamination, and strict aseptic sampling can be performed even though the frequent or aseptic sampling may still exist.
Disclosure of utility model
The utility model aims to provide a biological bacterial liquid fermentation reaction tank capable of estimating the number of bacterial colonies, so as to solve the problems that the biological bacterial liquid is sampled at fixed time, the reaction tank is opened every time, the exposure of the microbial environment and the risk of external pollution can be caused, and even if strict aseptic operation is adopted, the defect of the possibility of microbial pollution still exists, and the use is convenient.
In order to achieve the above purpose, the technical scheme provided by the utility model is as follows:
The utility model relates to a biological bacterial liquid fermentation reaction tank capable of estimating the bacterial colony number, which comprises a reaction tank and a base arranged at the bottom end of the reaction tank, wherein a reaction chamber is arranged in the reaction tank;
the upper surface of transparent observation jar is provided with the control little pole, and two sets of are installed to the control little pole for the biological fungus liquid of water conservancy diversion dilution.
Preferably, an observation chamber is arranged in the transparent observation tank, a rubber movable adjusting sleeve is arranged on the upper surface of the transparent observation tank, two groups of rubber movable adjusting sleeves are arranged, and an extension movable pipe is arranged at the inner bottom end of each rubber movable adjusting sleeve.
Preferably, the rubber movable adjusting sleeve and the extension movable pipe are fixedly butted with the middle end of the control small rod, the inner bottom surface of the observation chamber is provided with a culture medium flat plate, the peripheral frame of the culture medium flat plate is provided with a limiting guard rail, and the inner end head of the control small rod is butted with the upper surface of the culture medium flat plate.
Preferably, a pump head is arranged on one side wall surface of the reaction chamber, the pump head corresponds to the observation chamber, one end of the sampling tube is provided with a drainage bent pipe, and the drainage bent pipe penetrates through the reaction tank and extends to the inside of the observation chamber.
Preferably, the end of the drainage bent pipe is of an L-shaped structure, and the end of the drainage bent pipe corresponds to the upper surface of the culture medium flat plate.
Preferably, the control small rod is internally provided with a dilution conduit, the outer end head of the control small rod is provided with a sealing end head, and one end of the sealing end head is provided with a threaded plug head.
Preferably, one port of the dilution conduit is connected with the threaded plug, and the other port of the dilution conduit is provided with a funnel slot.
Compared with the prior art, the technical scheme provided by the utility model has the following beneficial effects:
1. According to the biological bacterial liquid fermentation reaction tank capable of estimating the bacterial colony number, the sampling tube is used for sampling a part of biological bacterial liquid which is reacted in the reaction chamber through the pump head, the part of the biological bacterial liquid is connected with the outer port of the dilution conduit through the threaded plug, the sealing end is taken down, the dilution conduit is used for introducing the dilution liquid, the dilution liquid falls onto the biological bacterial liquid along the slotted hole of the funnel, after the bacterial colony is cultured for a period of time, the bacterial colony number growing on the culture medium is observed, and estimation of the bacterial colony number can help to determine whether the nutrient element supply in the culture medium is enough or not, and the adjustment or the supplementation of the components are needed, so that the biological bacterial liquid reaction in the reaction tank is facilitated, and the fermentation process is regulated to achieve the optimal product yield.
2. According to the biological bacterial liquid fermentation reaction tank capable of estimating the bacterial colony number, the rubber movable adjusting sleeve and the extension movable pipe are fixedly connected with the middle end of the control small rod, the rubber movable adjusting sleeve and the extension movable pipe are of rubber structures, the rotation adjustment of the control small rod can be realized by utilizing the elastic structure of rubber, the rotation length of the control small rod can be properly adjusted by the extension movable pipe, and the adjustment of the dropping position of diluent is facilitated.
Drawings
FIG. 1 is a schematic view of the overall interior plan of the present utility model;
FIG. 2 is a schematic diagram of the structure of the reaction tank of the present utility model;
FIG. 3 is a schematic plan view of the interior of the transparent observation tank of the present utility model;
Fig. 4 is a schematic view of the interior of the control stick of the present utility model.
In the figure: 1. a reaction tank; 2. a base; 3. a reaction chamber; 4. a transparent observation tank; 41. an observation chamber; 42. a rubber movable adjusting sleeve; 43. extending the movable tube; 44. limiting guard rails; 45. a medium plate; 5. a sampling tube; 51. a pump head; 52. a drainage elbow; 6. a control stick; 61. diluting the catheter; 62. sealing the end head; 63. a threaded plug; 64. a funnel slot.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
For a further understanding of the present utility model, the present utility model will be described in detail with reference to the drawings.
Referring to fig. 1, the biological bacteria liquid fermentation reaction tank capable of estimating the number of bacterial colonies comprises a reaction tank 1 and a base 2 arranged at the bottom end of the reaction tank 1, wherein a reaction chamber 3 is arranged in the reaction tank 1, a transparent observation tank 4 is arranged on one side surface of the reaction tank 1, the transparent observation tank 4 is of a transparent structure, a sampling tube 5 is arranged at one side end of the inside of the reaction chamber 3, the biological bacteria liquid reacted in the reaction chamber 3 is sampled into the transparent observation tank 4 through the sampling tube 5, the biological bacteria liquid is transferred through proper dilution and is cultivated under proper conditions, after the bacterial colonies grow on a culture medium for a period of time are cultivated, a control small rod 6 is arranged on the upper surface of the transparent observation tank 4, and two groups of control small rods 6 are arranged for guiding and diluting the biological bacteria liquid.
The utility model is further described below with reference to examples.
Referring to fig. 2 to 4, an observation chamber 41 is provided in the transparent observation tank 4, a rubber movable adjusting sleeve 42 is provided on the upper surface of the transparent observation tank 4, two sets of rubber movable adjusting sleeves 42 are provided, an extension movable tube 43 is provided at the inner bottom end of the rubber movable adjusting sleeve 42, the rubber movable adjusting sleeve 42 and the extension movable tube 43 are fixedly abutted to the middle end of the control small rod 6, a culture medium flat plate 45 is provided at the inner bottom surface of the observation chamber 41, a limit guard rail 44 is mounted on the peripheral frame of the culture medium flat plate 45, and the inner end of the control small rod 6 is abutted to the upper surface of the culture medium flat plate 45.
A pump head 51 is arranged on one side wall surface of the reaction chamber 3, the pump head 51 corresponds to the observation chamber 41, a drainage bent pipe 52 is arranged at one end of the sampling pipe 5, the drainage bent pipe 52 penetrates through the reaction tank 1 to extend into the observation chamber 41, the end of the drainage bent pipe 52 is of an L-shaped structure, the end of the drainage bent pipe 52 corresponds to the upper surface of the culture medium flat plate 45, a dilution conduit 61 is arranged in the control small rod 6, a sealing end 62 is arranged at the outer end of the control small rod 6, a threaded plug 63 is arranged at one end of the sealing end 62, one port of the dilution conduit 61 is connected with the threaded plug 63, and a funnel slot 64 is arranged at the other port of the dilution conduit 61.
In this embodiment, the sampling tube 5 samples a part of the biological bacterial liquid passing through the reaction chamber 3 through the pump head 51 to the upper surface of the culture medium flat plate 45, the screw plug 63 is connected with the outer port of the dilution conduit 61, the sealing end 62 is removed, the dilution conduit 61 is used for introducing the dilution liquid, the dilution liquid falls onto the biological bacterial liquid along the funnel slot 64, after a period of culture, the number of colonies growing on the culture medium is observed, the estimation of the number of colonies can help to determine whether the nutrient element supply in the culture medium is enough, which components need to be regulated or supplemented, the promotion of the biological bacterial liquid reaction in the reaction tank is facilitated, the regulation of the fermentation process is facilitated to achieve the optimal product yield, the rubber movable regulating sleeve 42 and the extension movable tube 43 are fixedly connected with the middle end of the control small rod 6, the rubber movable regulating sleeve 42 and the extension movable tube 43 are of a rubber structure, the rotation regulation of the control small rod 6 can be realized by using the elastic structure of rubber, the rotation length of the control small rod 6 can be properly regulated by the extension movable tube 43, and the dripping position of the dilution liquid is facilitated to be regulated.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a biological fungus liquid fermentation reaction jar of estimated bacterial colony quantity, includes retort (1) and installs base (2) in retort (1) bottom, its characterized in that: the method comprises the steps that a reaction chamber (3) is arranged in a reaction tank (1), a transparent observation tank (4) is arranged on one side surface of the reaction tank (1), the transparent observation tank (4) is of a transparent structure, a sampling tube (5) is arranged at one side end of the inside of the reaction chamber (3), biological bacteria liquid reacted in the reaction chamber (3) is sampled into the transparent observation tank (4) through the sampling tube (5), the biological bacteria liquid is transferred through proper dilution, and the biological bacteria liquid is cultured under proper conditions, and after the biological bacteria liquid is cultured for a period of time, the number of bacterial colonies growing on a culture medium is observed;
The upper surface of transparent observation jar (4) is provided with control little pole (6), and two sets of are installed to control little pole (6) for the biological fungus liquid of water conservancy diversion dilution.
2. The biological fluid fermentation reaction tank capable of estimating the number of bacterial colonies according to claim 1, wherein: the inside of transparent observation jar (4) is provided with observation room (41), and the upper surface of transparent observation jar (4) is provided with rubber activity adjustment sleeve (42), and rubber activity adjustment sleeve (42) are provided with two sets of, and the inner bottom end of rubber activity adjustment sleeve (42) is provided with extension movable tube (43).
3. The biological fluid fermentation reaction tank capable of estimating the number of bacterial colonies according to claim 2, wherein: the rubber movable adjusting sleeve (42) and the extension movable tube (43) are fixedly connected with the middle end of the control small rod (6), a culture medium flat plate (45) is arranged on the inner bottom surface of the observation chamber (41), a limit guard rail (44) is arranged on the peripheral frame of the culture medium flat plate (45), and the inner end of the control small rod (6) is connected with the upper surface of the culture medium flat plate (45) in a butt joint mode.
4. A biological fluid fermentation reaction tank capable of estimating the number of colonies according to claim 3, wherein: a pump head (51) is arranged on one side wall surface of the reaction chamber (3), the pump head (51) corresponds to the observation chamber (41), a drainage elbow (52) is arranged at one end of the sampling tube (5), and the drainage elbow (52) penetrates through the reaction tank (1) and extends into the observation chamber (41).
5. The biological fluid fermentation reaction tank capable of estimating colony count according to claim 4, wherein: the end of the drainage bent pipe (52) is of an L-shaped structure, and the end of the drainage bent pipe (52) corresponds to the upper surface of the culture medium flat plate (45).
6. The biological fluid fermentation reaction tank capable of estimating the number of bacterial colonies according to claim 1, wherein: the inside of control little pole (6) is provided with dilution pipe (61), and the outer end of control little pole (6) is provided with sealed end (62), and screw thread chock plug (63) are installed to one end of sealed end (62).
7. The biological fluid fermentation reaction tank capable of estimating colony count according to claim 6, wherein: one port of the dilution conduit (61) is connected with the threaded plug (63), and the other port of the dilution conduit (61) is provided with a funnel slot (64).
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN221071504U true CN221071504U (en) | 2024-06-04 |
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| GR01 | Patent grant |