CN220183293U

CN220183293U - Cell culture medium bottle

Info

Publication number: CN220183293U
Application number: CN202321631273.7U
Authority: CN
Inventors: 李倩; 贺胜华; 李鹏飞; 毛凯
Original assignee: Beijing Maitekerui Biotechnology Co ltd
Current assignee: Beijing Maitekerui Biotechnology Co ltd
Priority date: 2023-06-26
Filing date: 2023-06-26
Publication date: 2023-12-15
Anticipated expiration: 2033-06-26

Abstract

The utility model relates to a cell culture medium bottle, which belongs to the technical field of cell culture, and is characterized in that an additive component is arranged, an additive nutrient medicament is added into an additive box through an additive inlet pipe, a pull rod is pulled upwards to enable a connecting rod to drive a push plate to rise, negative pressure is generated in a pressure box, a one-way valve is opened, the pull rod is pressed downwards to enable the medicament in the additive box to enter the pressure box through a liquid inlet pipe, the medicament is extruded by the push plate and then enters a base bottle body through a liquid outlet pipe, a sealing cover is prevented from being directly opened, bacteria enter the inside to affect cell culture, air circulation to the base bottle body is facilitated through the arrangement of an air inlet component, meanwhile, sealing can be also facilitated, and multiple filtration is facilitated for the entering air through the arrangement of a first air filtering membrane and a second air filtering membrane.

Description

Cell culture medium bottle

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture medium bottle.

Background

The cell culture technique can be a simple single cell or a few differentiated multicellular from a single cell through mass culture, which is an essential link of the cloning technique, and the cell culture itself is the cloning of the cell. A large number of cells or their metabolites are obtained by cell culture. Since biological products are derived from cells, cell culture techniques can be said to be the most central and basic of biotechnology.

The utility model discloses a special culture medium bottle for cell culture, which comprises a bottle body, wherein the upper part of the bottle body is provided with a bottle opening, a bottle cap is detachably arranged on the bottle opening, a marking area is arranged on the bottle cap, and a balancing weight is fixedly arranged at the bottom of the bottle body. According to the special culture medium bottle for cell culture, the bottle cap is in threaded connection with the bottle mouth, and the bottle mouth and the bottle cap are sealed, so that the problem of pollution to the culture medium bottle mouth is avoided. Meanwhile, the first groove is formed in the upper surface of the bottle cap, the bottom surface of the first groove is ground, the ground surface is convenient for writing and coloring, the color and the digital mark are added, and the distinguishing is convenient; when the utility model is used, the balancing weights with different weights can be placed in the second groove according to the volume of the culture medium in the bottle, the use is flexible and convenient, and the problem of easy dumping can be effectively solved.

The above-mentioned existing devices also have the following disadvantages:

in the process of culturing cells in a culture bottle, various nutrients and medicines need to be given regularly, the prior art can only unscrew a bottle cap or insert a straw to add the nutrients, so that bacterial infection is easy to cause, and the survival rate of the cells is reduced, meanwhile, the prior cell culture bottle can only strengthen the gas exchange inside and outside the bottle body by unscrewing the bottle cap, then the pollution of culture solution is extremely easy to cause, and the gas exchange inside and outside the bottle body is unfavorable.

Disclosure of Invention

The present utility model is directed to a cell culture medium bottle, which solves the above-mentioned problems of the prior art.

The technical scheme of the utility model is as follows: including the base bottle body, be provided with the dosing module on the base bottle body, upper end one side intercommunication of base bottle body has the intake pipe, the upper end of intake pipe is provided with the subassembly that admits air, the dosing module is including setting up the pull rod in the middle of the top of base bottle body, the inner end fixedly connected with pressure cell of base bottle body, fixedly connected with connecting rod in the middle of the lower extreme of pull rod, the other end of connecting rod passes the top fixedly connected with push pedal of pressure cell, the intercommunication has the drain pipe in the middle of the lower extreme of pressure cell, install the check valve on the drain pipe, dosing module is still including being located the dosing box of base bottle body both sides.

Further, the upper ends of the two medicine adding boxes are respectively communicated with medicine feeding pipes, the two medicine feeding pipes are connected with sealing covers in a threaded manner, the lower ends of the two sides of the pressure box are respectively communicated with liquid feeding pipes, the two liquid feeding pipes are respectively communicated with one sides of the two medicine adding boxes, and the two liquid feeding pipes are respectively provided with one-way valves.

Further, the top of base bottle body with one side that the subassembly that admits air kept away from mutually is fixedly connected with slot board, the label tablet is inserted to one side of slot board.

Furthermore, the scale is installed to front end one side of base bottle body, L type draw-in groove has been seted up to the front end upside of base bottle body, the rear end upside fixedly connected with L type fixed plate of base bottle body.

Further, the subassembly that admits air including with intake pipe upper end threaded connection's seal tube, the upper end of seal tube is seted up flutedly, the inside sealing block that is provided with of recess, the seal tube lower extreme is circumference equidistance and leaves and be equipped with a plurality of first inlet ports, a plurality of the upper end of first inlet port all with the recess intercommunication, first through-hole has been seted up to the seal tube lower extreme, first through-hole is located a plurality of the lower extreme of first inlet port, and a plurality of first inlet port all with first through-hole intercommunication, the lower extreme of first through-hole with the inside intercommunication of intake pipe, the inside fixedly connected with first air filtering membrane that can dismantle of first through-hole.

Further, the second through-hole has been seted up to the upper end of sealing block, the inside of second through-hole can be dismantled fixedly connected with second air filtering membrane, the inside of sealing block is circumference equidistance and leaves and be equipped with a plurality of second inlet ports, and is a plurality of the upper end of second inlet port respectively with second through-hole intercommunication, the lower extreme of sealing block is circumference equidistance fixedly connected with a plurality of rubber blocks, a plurality of the rubber block respectively with a plurality of the second inlet port is corresponding, a plurality of all has seted up the intercommunicating pore on the rubber block, a plurality of the intercommunicating pore respectively with a plurality of the second inlet port is linked together, a plurality of the rubber block all with the bottom contact of recess, fixedly connected with draws the piece in the middle of the upper end of sealing block.

The utility model provides a cell culture medium bottle through improvement, and compared with the prior art, the cell culture medium bottle has the following improvement and advantages:

according to the utility model, the medicine adding component is arranged, the added nutrient medicine is added into the medicine adding box through the medicine inlet pipe, the pull rod is pulled upwards, the connecting rod drives the push plate to rise, negative pressure is generated in the pressure box, the one-way valve is opened, the pull rod is pressed downwards, the medicine in the medicine adding box enters the pressure box through the liquid inlet pipe, the medicine is extruded through the push plate and then enters the base bottle body through the liquid outlet pipe, the phenomenon that bacteria enter the base bottle body directly due to the fact that the sealing cover is opened is avoided, cell culture is influenced, air circulation to the base bottle body is facilitated through the arrangement of the air inlet component, meanwhile sealing can be performed, and multiple filtration of the entering air is facilitated through the arrangement of the first air filtering membrane and the second air filtering membrane.

Drawings

The utility model is further explained below with reference to the drawings and examples:

FIG. 1 is a schematic elevational view of the present utility model;

FIG. 2 is a schematic side view of the present utility model;

FIG. 3 is a schematic view showing the internal structure of the base bottle body according to the present utility model;

FIG. 4 is an enlarged schematic view of the structure of FIG. 1A according to the present utility model;

FIG. 5 is a schematic top view of a seal block according to the present utility model.

Reference numerals illustrate: 1. a base bottle body; 2. a slot plate; 3. a label plate; 4. an L-shaped clamping groove; 5. an L-shaped fixing plate; 6. a graduated scale; 7. a dosing assembly; 8. a pull rod; 9. a pressure cell; 10. a connecting rod; 11. a push plate; 12. a liquid outlet pipe; 13. a dosing box; 14. a medicine inlet pipe; 15. a liquid inlet pipe; 16. an air intake assembly; 17. a sealing cylinder; 18. a groove; 19. a first air inlet hole; 20. a first through hole; 21. a first air filtration membrane; 22. a sealing block; 23. a second air inlet hole; 24. a second through hole; 25. a second air filtration membrane; 26. a rubber block; 27. a communication hole; 28. pulling blocks; 29. and an air inlet pipe.

Detailed Description

The following detailed description of the present utility model will provide clear and complete description of the technical solutions of the embodiments of the present utility model, with reference to fig. 1 to 5, and it is obvious that the described embodiments are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The utility model provides a cell culture medium bottle through improvement, as shown in fig. 1-5, the cell culture medium bottle comprises a base bottle body 1, a dosing assembly 7 is arranged on the base bottle body 1, one side of the upper end of the base bottle body 1 is communicated with an air inlet pipe 29, the upper end of the air inlet pipe 29 is provided with an air inlet assembly 16, the dosing assembly 7 comprises a pull rod 8 arranged in the middle of the top of the base bottle body 1, the inner end of the base bottle body 1 is fixedly connected with a pressure box 9, the middle of the lower end of the pull rod 8 is fixedly connected with a connecting rod 10, the other end of the connecting rod 10 is fixedly connected with a push plate 11 through the top of the pressure box 9, the middle of the lower end of the pressure box 9 is communicated with a liquid outlet pipe 12, a one-way valve is arranged on the liquid outlet pipe 12, the dosing assembly 7 also comprises a dosing box 13 positioned at two sides of the base bottle body 1, when nutrient substances are required to be added, the nutrient substances are added into the dosing box 13 from the liquid inlet pipe 14, the pull rod 8 is held, the connecting rod 10 is driven to move upwards, the push plate 11 is driven to move upwards, negative pressure is generated in the pressure box 9, the pressure box 9 is driven to move upwards, a liquid is driven to be filled into the pressure box 9, a liquid is driven to be filled into the base bottle body through the connecting rod 10, a liquid inlet pipe 10 is driven to move the connecting rod 10 is driven to move upwards, and then the nutrient solution is driven to move the nutrient solution to be filled into the nutrient box body through the bottle body through the connecting rod 10, and is driven to move 13, and is driven to move, and is filled into the nutrient is filled.

The upper ends of the two medicine feeding boxes 13 are respectively communicated with medicine feeding pipes 14, the two medicine feeding pipes 14 are connected with sealing covers in a threaded manner, the lower ends of two sides of the pressure box 9 are respectively communicated with liquid feeding pipes 15, the two liquid feeding pipes 15 are respectively communicated with one sides of the two medicine feeding boxes 13, and one-way valves are respectively arranged on the two liquid feeding pipes 15 so as to facilitate feeding.

One side that the top of basic bottle body 1 and the subassembly 16 that admits air kept away from mutually fixedly connected with slot board 2, one side of slot board 2 is inserted and is equipped with tag 3, is convenient for mark basic bottle body 1 cell, categorised.

The scale 6 is installed to front end one side of basic bottle body 1, and L type draw-in groove 4 has been seted up to the front end upside of basic bottle body 1, and the rear end upside fixedly connected with L type fixed plate 5 of basic bottle body 1 is convenient for connect a plurality of basic bottle bodies 1, inserts L type fixed plate 5 of another basic bottle body 1 into L type draw-in groove 4, is convenient for connect basic bottle body 1, saves space.

The air inlet assembly 16 comprises a sealing cylinder 17 in threaded connection with the upper end of an air inlet pipe 29, a groove 18 is formed in the upper end of the sealing cylinder 17, a sealing block 22 is arranged in the groove 18, a plurality of first air inlet holes 19 are formed in the lower end of the sealing cylinder 17 in a circumferential equidistant manner, the upper ends of the plurality of first air inlet holes 19 are all communicated with the groove 18, a first through hole 20 is formed in the lower end of the sealing cylinder 17, the first through hole 20 is located at the lower end of the plurality of first air inlet holes 19, the plurality of first air inlet holes 19 are all communicated with the first through hole 20, the lower end of the first through hole 20 is communicated with the inside of the air inlet pipe 29, a first air filtering membrane 21 is detachably and fixedly connected to the inside of the first through hole 20, and multiple filtration is conveniently carried out on air by arranging the first air filtering membrane 21 and the second air filtering membrane 25.

The upper end of the sealing block 22 is provided with a second through hole 24, a second air filtering membrane 25 is detachably and fixedly connected to the inside of the second through hole 24, a plurality of second air inlets 23 are formed in the sealing block 22 at equal intervals, the upper ends of the plurality of second air inlets 23 are respectively communicated with the second through hole 24, the lower end of the sealing block 22 is fixedly connected with a plurality of rubber blocks 26 at equal intervals, the plurality of rubber blocks 26 are respectively corresponding to the plurality of second air inlets 23, the plurality of rubber blocks 26 are respectively provided with a communication hole 27, the plurality of communication holes 27 are respectively communicated with the plurality of second air inlets 23, the plurality of rubber blocks 26 are respectively contacted with the bottoms of the grooves 18, a pull block 28 is fixedly connected to the middle of the upper end of the sealing block 22, the second air inlets 23, the communication holes 27 and the first air inlets 19 are correspondingly arranged through rotating the sealing block 22, so that the sealing block 22 is rotated when sealing is needed, the rubber blocks 26 are driven to rotate, the rubber blocks 26 and the first air inlets 19 are staggered and are conveniently sealed, and multiple filtering is facilitated through the arrangement of the first air inlets 21 and the second air filtering membrane 25.

Working principle: when nutrients are required to be added, the nutrient substances are added into the dosing box 13 from the dosing tube 14, the pull rod 8 is held, the pull rod 8 drives the connecting rod 10 to move upwards, the connecting rod 10 drives the push plate 11 to move upwards, the push plate 11 drives to move upwards so that negative pressure is generated in the pressure box 9, nutrient solution in the dosing box 13 is sucked into the pressure box 9, then the pull rod 8 is pressed downwards, the pull rod 8 drives the connecting rod 10 to move downwards, the connecting rod 10 drives the push plate 11 to move downwards, the push plate 11 can discharge the nutrient substances in the pressure box 9 into the base bottle body 1 from the liquid outlet tube 12, so that the nutrient substances are added to cells in the base bottle body 1, when air is required to enter the base bottle body 1, the second air inlet hole 23, the communication hole 27 and the first air inlet hole 19 are correspondingly formed through rotating the sealing block 22, the sealing block 22 is convenient to enter the air, the rubber block 26 is driven to rotate, the rubber block 26 and the first air inlet hole 19 are staggered for sealing, and the air filtering film 25 is convenient to carry out multiple filtering through the arrangement of the first air filtering film 21 and the second air filtering film 25.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A cell culture medium bottle, characterized in that: including basic bottle body (1), be provided with medicine adding component (7) on basic bottle body (1), upper end one side intercommunication of basic bottle body (1) has intake pipe (29), the upper end of intake pipe (29) is provided with air intake assembly (16), medicine adding component (7) are including setting up pull rod (8) in the middle of the top of basic bottle body (1), the inside fixedly connected with pressure box (9) of basic bottle body (1), fixedly connected with connecting rod (10) in the middle of the lower extreme of pull rod (8), the other end of connecting rod (10) passes top fixedly connected with push pedal (11) of pressure box (9), the intercommunication has drain pipe (12) in the middle of the lower extreme of pressure box (9), install the check valve on drain pipe (12), medicine adding component (7) still including being located medicine adding box (13) of basic bottle body (1) both sides.

2. A cell culture medium bottle according to claim 1, wherein: the upper ends of the two dosing boxes (13) are respectively communicated with a dosing pipe (14), the two dosing pipes (14) are connected with sealing covers in a threaded manner, the lower ends of the two sides of the pressure box (9) are respectively communicated with a liquid inlet pipe (15), the two liquid inlet pipes (15) are respectively communicated with one sides of the two dosing boxes (13), and the two liquid inlet pipes (15) are respectively provided with one-way valves.

3. A cell culture medium bottle according to claim 2, wherein: the top of basic bottle body (1) with one side that subassembly (16) that admits air kept away from mutually is fixedly connected with slot board (2), one side of slot board (2) is inserted and is equipped with tag (3).

4. A cell culture medium bottle according to claim 1, wherein: the utility model discloses a bottle, including base bottle body (1), scale (6) are installed to front end one side of base bottle body (1), L type draw-in groove (4) have been seted up to front end upside of base bottle body (1), rear end upside fixedly connected with L type fixed plate (5) of base bottle body (1).

5. A cell culture medium bottle according to claim 1, wherein: the utility model provides a novel air filter, including intake subassembly (16), intake subassembly (16) including with sealing tube (17) of intake pipe (29) upper end threaded connection, fluted (18) are seted up to the upper end of sealing tube (17), inside sealing block (22) that are provided with of recess (18), sealing tube (17) lower extreme is circumference equidistance and leaves and be equipped with a plurality of first inlet port (19), a plurality of the upper end of first inlet port (19) all with recess (18) intercommunication, first through-hole (20) have been seted up to sealing tube (17) lower extreme, first through-hole (20) are located a plurality of the lower extreme of first inlet port (19), and a plurality of first inlet port (19) all with first through-hole (20) intercommunication, the lower extreme of first through-hole (20) with intake pipe (29) inside intercommunication, the inside detachably fixedly connected with first air filtering membrane (21) of first through-hole (20).

6. A cell culture medium bottle according to claim 5, wherein: the upper end of sealing block (22) has seted up second through-hole (24), the inside of second through-hole (24) can be dismantled fixedly connected with second air filtration membrane (25), the inside of sealing block (22) is circumference equidistance and leaves and be equipped with a plurality of second inlet port (23), a plurality of the upper end of second inlet port (23) respectively with second through-hole (24) intercommunication, the lower extreme of sealing block (22) is circumference equidistance fixedly connected with a plurality of rubber piece (26), a plurality of rubber piece (26) respectively with a plurality of second inlet port (23) are corresponding, a plurality of communication hole (27) have all been seted up on rubber piece (26), a plurality of communication hole (27) respectively with a plurality of second inlet port (23) are linked together, a plurality of rubber piece (26) all with the bottom contact of recess (18), the upper end intermediate fixedly connected with of sealing block (22) draws piece (28).