CN218372341U

CN218372341U - Cell culture bottle

Info

Publication number: CN218372341U
Application number: CN202222812069.7U
Authority: CN
Inventors: 韩红艳
Original assignee: Jinzhong University
Current assignee: Zhongchuang Longsheng Pharmaceutical Biotechnology (Shanxi) Co.,Ltd.
Priority date: 2022-10-25
Filing date: 2022-10-25
Publication date: 2023-01-24
Anticipated expiration: 2032-10-25

Abstract

The utility model discloses a cell culture bottle, including fixed case, fixedly connected with motor on the inner wall of fixed case, the reciprocating type thick stick of output fixedly connected with of motor, the symmetrical meshing is connected with two reciprocating type thick stick covers on the reciprocating type thick stick, every the equal fixedly connected with montant of one side of reciprocating type thick stick cover, two slide openings, two have been seted up to the up end symmetry of fixed case the slide opening and common fixedly connected with bottle all run through to the upper end of montant, the straight section of thick bamboo of fixedly connected with in the bottle. The utility model can drive the bottle body to swing left and right through the vertical rod through the matching arrangement of the motor, the reciprocating type rod and the reciprocating type rod sleeve, thereby preventing the cells in the bottle body from being gathered together; through the cooperation setting of slide, push rod and siphunculus, can follow and inhale nutritive substance in the containing box, need not unscrew the bottle lid or insert the straw, reduce bacterial infection's probability, and then improve the survival rate of cell.

Description

Cell culture bottle

Technical Field

The utility model relates to a blake bottle technical field especially relates to a cell culture bottle.

Background

In vitro culture of cells and tissues has become an essential element in life research and practice, with a wide variety of cell types ranging from viruses to bacteria and fungi, and from human cells to animal and plant cells. Some cells and tissues can grow in suspension, but a significant proportion of mammalian cells require surface adherence. The cell culture flask is used for cell culture, and can obtain a large amount of experimental data about basic growth information and culture process parameters of cells under the conditions of limited manpower, material resources and space, so the cell culture flask is widely applied to laboratory research and industrial production.

In the process of culturing cells in a culture bottle, a plurality of nutrient substances and medicines are required to be regularly administered, and in the prior art, the bottle cap is unscrewed or a straw is inserted to add the nutrient substances, so that bacterial infection is easily caused, and the survival rate of the cells is reduced.

In order to solve the problems, a cell culture bottle is provided.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the problem that in the prior art, multiple nutrients and medicines are required to be regularly given to cells in the culture bottle culture process, the prior art is that the bottle cap is unscrewed or the straw is inserted to add nutrients, and the bacterial infection is easily caused by the prior art, so that the survival rate of the cells is reduced, and the cell culture bottle is provided.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a cell culture bottle, includes fixed case, fixedly connected with motor on the inner wall of fixed case, the reciprocating type thick stick of output fixedly connected with of motor, the symmetrical meshing is connected with two reciprocating type thick stick covers on the reciprocating type thick stick, every the equal fixedly connected with montant in one side of reciprocating type thick stick cover, two slide holes, two have been seted up to the up end symmetry of fixed case the upper end of montant all runs through slide hole and common fixedly connected with bottle, the straight section of thick bamboo of fixedly connected with in the bottle, the lower terminal surface fixedly connected with discharging pipe of straight section of thick bamboo, fixedly connected with exhaust check valve in the discharging pipe, sliding connection has the slide in the straight section of thick bamboo, the up end fixedly connected with push rod of slide, two of the symmetrical two siphunculus of left and right sides of straight section of thick bamboo the one end that the siphunculus is relative all runs through the straight section of thick bamboo and is linked together with the straight section of thick bamboo, two containing boxes of left and right sides symmetry fixedly connected with of bottle, two the siphunculus one end back of the body all runs through the lateral wall of bottle and containing box and communicates with the containing box mutually, every the siphunculus is the equal fixedly connected with check valve that admits air in the one end in containing box.

Preferably, the equal fixedly connected with filling tube of up end of containing box, every the equal meshing of upper end of filling tube is connected with sealed lid, every equal fixedly connected with filter screen in the filling tube.

Preferably, the upper end surface of the bottle body is symmetrically and fixedly connected with two air inlet pipes, and the lower end of each air inlet pipe penetrates through the upper end surface of the bottle body and is communicated with the bottle body.

Preferably, the upper end of the push rod penetrates through the upper end face of the bottle body and is fixedly connected with the handle, and the push rod is connected in the inner wall of the bottle body in a sliding mode.

Preferably, one end of the reciprocating bar, which is far away from the motor, is rotatably connected to the inner wall of the fixed box, and the two vertical bars are connected to the sliding hole in a sliding mode.

Preferably, the right side of the bottle body is fixedly connected with a liquid discharge pipe, and the liquid discharge pipe is fixedly connected with a valve.

Compared with the prior art, the beneficial effects of the utility model are that:

through the matching arrangement of the motor, the reciprocating lever and the reciprocating lever sleeve, the bottle body can be driven by the vertical rod to swing left and right, so that cells in the bottle body are prevented from being gathered together; through the cooperation setting of slide, push rod and siphunculus, can follow and inhale nutritive substance in the containing box, need not unscrew the bottle lid or insert the straw, reduce bacterial infection's probability, and then improve the survival rate of cell.

Drawings

FIG. 1 is a schematic view of a front three-dimensional structure of a cell culture bottle according to the present invention;

FIG. 2 is a schematic diagram of a front cross-sectional structure of a cell culture bottle according to the present invention.

In the figure: the device comprises a fixed box 1, a motor 2, a reciprocating lever 3, a reciprocating lever sleeve 4, a vertical rod 5, a bottle 6, a straight cylinder 7, a sliding plate 8, a push rod 9, a handle 10, a containing box 11, a charging pipe 12, a filter screen 13, a through pipe 14, an air inlet check valve 15, a discharging pipe 16, an air inlet pipe 17 and a liquid discharge pipe 18.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-2, a cell culture bottle, including a fixed box 1, a motor 2 is fixedly connected to an inner wall of the fixed box 1, a reciprocating lever 3 is fixedly connected to an output end of the motor 2, two reciprocating lever sleeves 4 are symmetrically engaged and connected to the reciprocating lever 3, a vertical rod 5 is fixedly connected to one side of each reciprocating lever sleeve 4, two sliding holes are symmetrically formed in an upper end surface of the fixed box 1, upper ends of the two vertical rods 5 penetrate through the sliding holes and are fixedly connected to a bottle 6 together, a straight tube 7 is fixedly connected to the inside of the bottle 6, a discharge tube 16 is fixedly connected to a lower end surface of the straight tube 7, an exhaust check valve is fixedly connected to the inside of the discharge tube 16, a sliding plate 8 is slidably connected to the straight tube 7, a push rod 9 is fixedly connected to an upper end surface of the sliding plate 8, two through tubes 14 are symmetrically fixedly connected to left and right sides of the straight tube 7, opposite ends of the two through tubes 14 penetrate through the straight tube 7 and are communicated with the straight tube 7, two containing boxes 11 are symmetrically fixedly connected to left and right sides of the bottle 6, opposite ends of the two containing boxes 14, two containing boxes 11 are fixedly connected to each through the side walls of the containing box 11, one end of each through the fixed to the corresponding one of the containing box 14, the fixed to the corresponding one through tube 4, the corresponding one of the corresponding containing box, the bottle 6, the corresponding through tube 6, the corresponding one through tube 15, the bottle 2 and the corresponding to prevent the bottle 5 from shaking of the reciprocating lever sleeve 6, the bottle 2, the bottle 6, and the bottle 6, and the corresponding bar 6, the bottle 6, and prevent the bottle from shaking bar 6; through the cooperation setting of slide 8, push rod 9 and siphunculus 14, can inhale nutritive substance from containing box 11, need not unscrew the bottle lid or insert the straw, reduce bacterial infection's probability, and then improve the survival rate of cell.

Wherein, the equal fixedly connected with filling tube 12 of up end of two containing box 11, the equal meshing in upper end of every filling tube 12 is connected with sealed lid, equal fixedly connected with filter screen 13 in every filling tube 12, and filter screen 13 can prevent that dust impurity from entering into containing box 11 in to the nutrient solution cause the pollution.

Wherein, two intake pipes 17 of the up end symmetry fixedly connected with of bottle 6, the lower extreme of every intake pipe 17 all runs through the up end of bottle 6 and is linked together with bottle 6, can follow and add oxygen in the intake pipe 17.

Wherein, the upper end of push rod 9 runs through the up end of bottle 6 and fixedly connected with handle 10, and push rod 9 sliding connection is in the inner wall of bottle 6, and push rod 9 can drive slide 8 and remove.

Wherein, reciprocating type thick stick 3 deviates from the one end rotation connection of motor 2 on the inner wall of fixed case 1, and two equal sliding connection of montant 5 are in the slide opening, and reciprocating type thick stick 3 can drive reciprocating type thick stick cover 4 and remove.

Wherein, the right side of bottle 6 is fixedly connected with a liquid discharge pipe 18, and a valve is fixedly connected on the liquid discharge pipe 18, so that waste liquid can be discharged from the liquid discharge pipe 18.

In the utility model, when nutrient substances are required to be added, the nutrient substances are added into the containing box 11 from the feeding pipe 12, the handle 10 is held, the handle 10 drives the push rod 9 to move upwards, the push rod 9 drives the slide plate 8 to move upwards, the slide plate 8 drives the slide plate 8 to move upwards, so that negative pressure can be generated in the straight cylinder 7, nutrient solution in the containing box 11 is sucked into the straight cylinder 7, then the handle 10 is pressed downwards, the handle 10 drives the push rod 9 to move downwards, the push rod 9 drives the slide plate 8 to move downwards, the slide plate 8 can discharge the nutrient substances in the straight cylinder 7 into the bottle body 6 from the discharge pipe 16, and the nutrient substances are added to cells in the bottle body 6; the motor 2 is started, the output end of the motor drives the reciprocating lever 3 to rotate, the reciprocating lever 3 drives the reciprocating lever sleeve 4 to move left and right, the reciprocating lever sleeve 4 drives the vertical rod 5 to move left and right, and the vertical rod 5 drives the bottle body 6 to move left and right, so that cells in the bottle body 6 are prevented from being accumulated together; through the matching arrangement of the motor 2, the reciprocating lever 3 and the reciprocating lever sleeve 4, the bottle body 6 can be driven to swing left and right through the vertical rod 5, so that cells in the bottle body 6 are prevented from being aggregated together; through the cooperation setting of slide 8, push rod 9 and siphunculus 14, can inhale nutrient substance from containing box 11, need not unscrew the bottle lid or insert the straw, reduce bacterial infection's probability, and then improve the survival rate of cell.

The above, only be the embodiment of the preferred of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, which are designed to be replaced or changed equally, all should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a cell culture bottle, includes fixed case (1), its characterized in that, fixedly connected with motor (2) on the inner wall of fixed case (1), the reciprocating type thick stick (3) of output fixedly connected with of motor (2), the symmetry meshing is connected with two reciprocating type thick stick cover (4) on reciprocating type thick stick (3), every reciprocating type thick stick cover (4) one side equal fixedly connected with montant (5), two slide openings have been seted up to the up end symmetry of fixed case (1), two slide openings and common fixedly connected with bottle (6) are all run through to the upper end of montant (5), fixedly connected with straight section of thick bamboo (7) in bottle (6), the lower terminal surface fixedly connected with discharging pipe (16) of straight section of thick bamboo (7), fixedly connected with exhaust check valve in discharging pipe (16), sliding connection has slide (8) in straight section of thick bamboo (7), the up end fixedly connected with push rod (9) of slide (8), two left and right sides symmetry fixedly connected with of straight section of thick bamboo (7) have two siphunculus (14), two the relative one end of straight section of thick bamboo (14) all run through straight section of thick bamboo (7) and with the case lateral wall with two siphunculus (11) of the case lateral walls of containing box (11) are linked together, the box (11) are all the side wall of the two holding box (11) are linked together, the side of the holding box (11) are connected with the bottle (11) and the side wall of the bottle (11) and the holding box lateral wall (11) is held And an air inlet one-way valve (15) is fixedly connected to one end of each through pipe (14) in the storage box (11).

2. The cell culture bottle according to claim 1, wherein the upper end surfaces of the two containers (11) are fixedly connected with feed pipes (12), the upper end of each feed pipe (12) is engaged and connected with a sealing cover, and a filter screen (13) is fixedly connected in each feed pipe (12).

3. The cell culture bottle according to claim 1, wherein two air inlet pipes (17) are symmetrically and fixedly connected to the upper end surface of the bottle body (6), and the lower end of each air inlet pipe (17) penetrates through the upper end surface of the bottle body (6) and is communicated with the bottle body (6).

4. The cell culture bottle according to claim 1, wherein the upper end of the push rod (9) penetrates the upper end surface of the bottle body (6) and is fixedly connected with a handle (10), and the push rod (9) is slidably connected in the inner wall of the bottle body (6).

5. A cell culture flask according to claim 1, wherein the end of the reciprocating bar (3) facing away from the motor (2) is rotatably connected to the inner wall of the stationary box (1), and the two vertical bars (5) are slidably connected in slide holes.

6. The cell culture bottle according to claim 1, wherein a drain pipe (18) is fixedly connected to the right side of the bottle body (6), and a valve is fixedly connected to the drain pipe (18).