CN210695293U - Strain culture container - Google Patents

Strain culture container Download PDF

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Publication number
CN210695293U
CN210695293U CN201921614223.1U CN201921614223U CN210695293U CN 210695293 U CN210695293 U CN 210695293U CN 201921614223 U CN201921614223 U CN 201921614223U CN 210695293 U CN210695293 U CN 210695293U
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China
Prior art keywords
bottle
culture
bottle cap
filtering cylinder
cylinder body
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CN201921614223.1U
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Chinese (zh)
Inventor
陈强
黄巧珍
黄本素
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Tianhua Modern Agriculture Co ltd
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Tianhua Modern Agriculture Co ltd
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Abstract

The utility model relates to a strain culture container, belonging to the field of culture containers, comprising a culture bottle; the culture bottle also comprises a bottle cap component which is covered at the opening at the upper end of the culture bottle; the bottle cap component comprises a bottle cap in threaded connection with the culture bottle, and a hollow filtering cylinder body which is coaxially arranged in the bottle cap; the upper end of the filtering cylinder is in threaded connection with the inner wall of the bottle cap; after the bottle cap and the culture bottle are installed in place, the bottom end of the filtering cylinder body extends to the upper part of the bottom wall of the culture bottle, and a cavity for air circulation is formed between the filtering cylinder body and the culture bottle; a plurality of vent holes are formed in the side wall of the filtering cylinder body; the middle part of the bottle cap is provided with an air hole. The circulation of air and moisture in the culture bottle is ensured to be smooth, when the sporocarp is mature, the filtering cylinder is driven to be taken out through the bottle cap by suspending the loose bottle cap and collecting the mushrooms, so that the mushrooms can be directly removed manually, and the time and the labor are saved; after the manual removal, the filtering cylinder body and the culture bottle are convenient to clean.

Description

Strain culture container
Technical Field
The utility model relates to a culture vessel field, more specifically relates to a bacterial culture vessel.
Background
The mother culture process of fungus mushroom, need a large amount of culture containers, like the culture container of the cultivation adoption to crab taste mushroom, be CN203801356U like patent publication, the name is a special cultivation bottle of crab taste mushroom, though solved the later stage and gone out the mushroom because the interval undersize between bottle and the bottle, the mutual bottle crowds of crab taste mushroom fruit body, be unfavorable for the circulation of air, can cause partial mushroom body mushroom shape to damage simultaneously, be unfavorable for the oxygen transport of bottom culture material in the bottle and the timely emission problem of carbon dioxide, its nutrient substance of this application is filled in the bottle, lead to its inside air and moisture circulation not smooth, its cultivation bottle is difficult for the sanitization. Therefore, effective solutions to solve the above problems need to be proposed.
SUMMERY OF THE UTILITY MODEL
In order to overcome the defects of the prior art, the utility model discloses a strain culture container, the difficult sanitization's of inside air and moisture circulation problem in the solution technique, its cultivation bottle.
To achieve the purpose, the utility model adopts the following technical proposal:
the utility model provides a strain culture container, which comprises a culture bottle;
the culture bottle also comprises a bottle cap component which is covered at the opening at the upper end of the culture bottle; the bottle cap assembly comprises a bottle cap in threaded connection with the culture bottle, and the hollow filtering cylinder body is coaxially arranged in the bottle cap; the upper end of the filtering cylinder is in threaded connection with the inner wall of the bottle cap; after the bottle cap and the culture bottle are installed in place, the bottom end of the filtering cylinder body extends to the position above the bottom wall of the culture bottle, and a cavity for air circulation is formed between the filtering cylinder body and the culture bottle; a plurality of vent holes are formed in the side wall of the filtering cylinder body; the middle part of the bottle cap is provided with an air hole.
As a further improvement of the technical proposal, the bottle neck of the culture bottle is in a straight cylinder shape, and the bottle body of the culture bottle is provided with an arc shape which is inwards sunk towards the vertical central axis direction of the culture bottle.
As a further improvement of the technical scheme, the ratio of the diameter of the bottle body to the diameter of the bottle neck is 1.5:1-1.6: 1.
As a further improvement of the technical scheme, the air holes are filled with porous air-permeable sponge.
As a further improvement of the technical scheme, the bottom of the filtering cylinder body is provided with a necking.
As a further improvement of the technical scheme, the culture bottle and the bottle cap assembly are made of PET materials.
The utility model has the advantages that:
the utility model relates to a strain culture container, a cavity for air circulation is arranged between a filtering cylinder and a culture bottle, and is filled with anhydrous or a small amount of water, the water is used as a gas storage layer to provide enough oxygen for hypha culture, and the oxygen provides necessary oxygen for hypha growth through the cavity or an air vent; wherein with bottle lid and blake bottle threaded connection, filter the upper end of barrel and the inner wall threaded connection of bottle lid, can guarantee the bottle lid and filter the barrel to and the stability that bottle lid and blake bottle are connected, avoid opening because of the accident and lead to external bacterial to invade in the bottle. Therefore, the nutrient medium is filled in the filtering cylinder body, the filtering cylinder body can control the fruiting bodies of the beech mushrooms in the filtering cylinder body, the fruiting bodies are compact, uniform in size and attractive in appearance, when the fruiting bodies are mature, the filtering cylinder body is driven to be taken out through the bottle cap by loosening the bottle cap in a hanging mode, the mushrooms are collected, and the time and labor can be saved by manually and directly picking the mushrooms; after the manual removal, the filtering cylinder body and the culture bottle are convenient to clean.
Drawings
FIG. 1 is a schematic front view of a bacterial culture container according to an embodiment of the present invention;
fig. 2 is an enlarged schematic structural diagram of a point a provided in the embodiment of the present invention.
In the figure:
1. a culture bottle; 11. a bottleneck; 12. a bottle body; 2. a bottle cap assembly; 21. a bottle cap; 211. air holes are formed; 22. a filter cylinder; 221. a vent hole; 100. a cavity.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.
The utility model provides a strain culture container, as shown in figure 1-2; comprises a culture bottle 1; the culture bottle also comprises a bottle cap component 2 which is covered at the opening at the upper end of the culture bottle 1; the bottle cap component 2 comprises a bottle cap 21 in threaded connection with the culture bottle 1 and a hollow filtering cylinder 22 coaxially arranged in the bottle cap 21; the upper end of the filtering cylinder 22 is in threaded connection with the inner wall of the bottle cap 21; after the bottle cap 21 and the culture bottle 1 are installed in place, the bottom end of the filter cylinder 22 extends to the upper part of the bottom wall of the culture bottle 1, and a cavity 100 for air circulation is formed between the filter cylinder 22 and the culture bottle 1; thereby ensuring that the cavity 100 is communicated with the inner cavity of the culture bottle 1 and the filtering cylinder 22, and the side wall of the filtering cylinder 22 is provided with a plurality of vent holes 221; the middle part of the bottle cap 21 is provided with a vent hole 211. Specifically, when the Hypsizygus marmoreus is planted, the nutrient substances and the water are set in proportion, the breathable water-retaining material is added into the culture bottle 1, then the culture material is placed into the filter cylinder 22, the culture material is sterilized by a vacuum autoclave at 121 ℃ for 1.5 hours, the culture period of hyphae enters after inoculation, no water or a small amount of water is filled in a cavity 100 for air circulation between the filter cylinder 22 and the culture bottle 1, the water is used as a gas storage layer to provide enough oxygen for hyphae culture, and the oxygen provides necessary oxygen for hyphae growth through the cavity 100 or the air vent 221. Wherein with bottle lid 21 and 1 threaded connection of blake bottle, filter the upper end of barrel 22 and the inner wall threaded connection of bottle lid 21, can guarantee that bottle lid 21 filters barrel 22 to and the stability that bottle lid 21 and blake bottle 1 are connected, avoid opening because of the accident and lead to external bacterial to invade in the bottle. Therefore, the hollow filtering cylinder 22 is coaxially arranged inside the bottle cap 21, and the upper end of the filtering cylinder 22 is in threaded connection with the inner wall of the bottle cap 21, wherein the nutrition medium is filled inside the filtering cylinder 22, the filtering cylinder 22 can control the fruiting bodies of the beech mushrooms in the filtering cylinder 22, the fruiting bodies are compact, uniform and attractive in size, when the fruiting bodies are mature, the bottle cap 21 is loosened in a hanging manner, the filtering cylinder 22 is driven by the bottle cap 21 to be taken out and the mushrooms are collected, the manual operation can be directly carried out, the time and the labor are saved, and the filtering cylinder 22 and the culture bottle 1 can be conveniently cleaned after the manual operation.
Further, the neck 11 of the culture bottle 1 is a straight cylinder, and the body 12 of the culture bottle 1 has an arc shape recessed inward toward the vertical central axis of the culture bottle 1. The bottle body 12 is provided with an inward arc shape, so that gaps between adjacent bottles are increased, air circulation is increased, and thorough sterilization is facilitated. And the inward side wall can facilitate the grabbing of the bottle body 12.
Further, the ratio of the diameter of the bottle body 12 to the diameter of the bottle neck 11 is 1.5:1-1.6: 1. Can make the interval increase between the body 12, in the fruiting stage of later stage, prevent that the mushroom entity of crab flavor is crowded each other, be favorable to the circulation of air.
Furthermore, the air holes 211 are filled with porous sponge, so that the air holes can filter mixed bacteria and are fully suitable for the growth and development of strains.
Further, the bottom of the filter cylinder 22 is provided as a throat. Wherein nutrient medium fills inside filtering barrel 22, filters barrel 22 and can control the fruit body of crab flavor mushroom in filtering barrel 22, guarantees that the compact size of fruit body is consistent and pleasing to the eye, and when the fruit body is ripe back, through hanging loose bottle lid 21, drives through bottle lid 21 and filters barrel 22 and take out and receive the mushroom, filters barrel 22's bottom and sets to the throat for nutrient medium receives the extrusion, and is difficult for dropping.
Further, the culture bottle 1 and the bottle cap assembly 2 are made of PET material. The PET is highly transparent, facilitates the observation of the growth of hyphae, is nontoxic and odorless, has proper hardness, and does not influence the growth of the hypsizygus marmoreus.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the spirit and scope of the invention. The present invention is not to be limited by the specific embodiments disclosed herein, and other embodiments that fall within the scope of the claims of the present application are intended to be within the scope of the present invention.

Claims (6)

1. A strain culture container comprises a culture bottle (1); the method is characterized in that:
the culture bottle is characterized by also comprising a bottle cap component (2) which is covered at an opening at the upper end of the culture bottle (1); the bottle cap assembly (2) comprises a bottle cap (21) in threaded connection with the culture bottle (1), and a hollow filtering cylinder body (22) which is coaxially arranged in the bottle cap (21); the upper end of the filtering cylinder body (22) is in threaded connection with the inner wall of the bottle cap (21); after the bottle cap (21) and the culture bottle (1) are installed in place, the bottom end of the filter cylinder body (22) extends to the position above the bottom wall of the culture bottle (1), and a cavity (100) for air circulation is formed between the filter cylinder body (22) and the culture bottle (1); a plurality of vent holes (221) are formed in the side wall of the filtering cylinder body (22); the middle part of the bottle cap (21) is provided with an air hole (211).
2. A strain culture vessel according to claim 1, wherein:
the bottleneck (11) of blake bottle (1) become straight tube-shape, have on the body (12) of blake bottle (1) towards the arc type of the vertical axis direction invagination of blake bottle (1).
3. A strain culture vessel according to claim 2, wherein:
the ratio of the diameter of the bottle body (12) to the diameter of the bottle neck (11) is 1.5:1-1.6: 1.
4. A strain culture vessel according to claim 1, wherein:
porous breathable sponge is filled at the positions of the breathable holes (211).
5. A strain culture vessel according to claim 1, wherein:
the bottom of the filtering cylinder body (22) is provided with a necking.
6. A strain culture vessel according to claim 1, wherein:
the culture bottle (1) and the bottle cap component (2) are made of PET materials.
CN201921614223.1U 2019-09-26 2019-09-26 Strain culture container Active CN210695293U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201921614223.1U CN210695293U (en) 2019-09-26 2019-09-26 Strain culture container

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201921614223.1U CN210695293U (en) 2019-09-26 2019-09-26 Strain culture container

Publications (1)

Publication Number Publication Date
CN210695293U true CN210695293U (en) 2020-06-09

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CN201921614223.1U Active CN210695293U (en) 2019-09-26 2019-09-26 Strain culture container

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111699926A (en) * 2020-07-06 2020-09-25 湖南果秀食品有限公司 Fungus bottle body for inoculating pleurotus eryngii with liquid seeds and comprehensive fungus bottle culture method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111699926A (en) * 2020-07-06 2020-09-25 湖南果秀食品有限公司 Fungus bottle body for inoculating pleurotus eryngii with liquid seeds and comprehensive fungus bottle culture method

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