CN220056878U - Multi-port cell culture bottle capable of sampling rapidly - Google Patents
Multi-port cell culture bottle capable of sampling rapidly Download PDFInfo
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- CN220056878U CN220056878U CN202321627159.7U CN202321627159U CN220056878U CN 220056878 U CN220056878 U CN 220056878U CN 202321627159 U CN202321627159 U CN 202321627159U CN 220056878 U CN220056878 U CN 220056878U
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- cell culture
- end cover
- bottle body
- side wall
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- 238000004113 cell culture Methods 0.000 title claims abstract description 50
- 238000005070 sampling Methods 0.000 title claims abstract description 39
- 238000003756 stirring Methods 0.000 claims abstract description 43
- 238000005192 partition Methods 0.000 claims abstract description 10
- 238000009423 ventilation Methods 0.000 claims description 12
- 230000002093 peripheral effect Effects 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 3
- 239000003550 marker Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 14
- 238000001514 detection method Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a multi-port cell culture flask capable of rapidly sampling, which comprises a flask body, a cross-shaped partition plate and four stirring rods. The cross-shaped partition board is arranged in the bottle body to uniformly divide the inner cavity of the bottle body into four independent culture cavities, and each culture cavity is correspondingly provided with a stirring rod; four first holes are formed in the top of the bottle body at intervals, and four second holes are formed in the middle upper portion of the side wall of the bottle body at intervals; the top end of the bottle body is covered with an end cover, and the inner wall of the end cover is in sliding connection with the side wall of the bottle body; and the top of the end cover is provided with a first bottleneck corresponding to the first hole, and the side wall of the end cover is provided with a second bottleneck corresponding to the second hole. According to the utility model, the end cover is rotated to sample different positions of different culture cavities, so that the number of bottle openings is reduced, and the bottle is convenient to take; the inner cavity of the bottle body is uniformly divided into four culture cavities by the cross-shaped partition plate, so that four different cells can be cultured in the bottle body at the same time; the culture solution in the corresponding culture cavity is stirred by rotating the stirring rod.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a multi-port cell culture bottle capable of rapidly sampling.
Background
In vitro culture of cells and tissues has become an essential element of life research and practice. The cell types cultivated are numerous, from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Some cells and tissues are grown in suspension and are placed in a cell culture flask for culturing.
During the process of culturing cells, sampling detection and comparison are required for different culture conditions or relevant parameters of different cells. At present, cells are mostly placed in a cell culture bottle and then placed in an incubator for culture under the same conditions; when sampling detection is needed, taking out the corresponding cell culture bottle, and then opening the cell culture bottle and sampling; after the sampling is finished, the cell culture bottle is put back into the incubator, and then the next cell culture bottle is taken out, or all the cell culture bottles are taken out together in the process of culturing cells, and sampling is carried out.
The cell culture bottle of current cell culture bottle is as this patent of current publication number CN206502828U discloses a cell culture bottle of convenient sample, cell culture bottle includes blake bottle body, isolation baffle, top end cover, upper portion end cover and lower part end cover, the blake bottle body includes body, top bottleneck, upper portion bottleneck and lower part bottleneck. When the cell culture bottle is used, a user can place different cells together in the culture bottle for culture, when sampling is carried out, the culture bottle is only required to be taken out, if the quantity is more, the quantity of the culture bottle can be increased again, time and labor are saved, the working time is greatly saved, the working efficiency is improved, the functions of a general cell culture bottle are achieved, the upper and lower different positions in the cell culture bottle can be rapidly sampled, and meanwhile, different cells can be placed in the culture bottle for culture.
However, the cell culture bottle has too many bottle openings, which is not beneficial for staff to take; the cell culture bottle is not provided with a stirring device, and the cell culture bottle is required to be taken for shaking, so that the operation is inconvenient; the cell culture bottle has no vent hole.
At present, no effective solution is proposed for solving the problems that the bottle mouth of the cell culture bottle in the related technology is too many, which is not beneficial to taking, no stirring device, no ventilation holes and the like.
Disclosure of Invention
The utility model aims to overcome the defects in the prior art, and provides a multi-port cell culture bottle capable of rapidly sampling so as to solve the problems that the bottle mouth on the cell culture bottle is too many, is unfavorable for taking, has no stirring device, has no air holes and the like in the related art.
In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:
a multi-port cell culture bottle of quick sample, includes bottle, cross baffle and four stirring rods, wherein:
the cross-shaped partition plate is arranged in the bottle body so as to divide the inner cavity of the bottle body into four independent culture cavities, and each culture cavity is correspondingly provided with a stirring rod;
four first holes are formed in the top of the bottle body at intervals, and four second holes are formed in the middle upper portion of the side wall of the bottle body at intervals; an end cover is covered at the top end of the bottle body, and the inner wall of the end cover is in sliding connection with the side wall of the bottle body; and the top of the end cover is provided with a first bottleneck corresponding to the first hole, and the side wall of the end cover is provided with a second bottleneck corresponding to the second hole.
Further, the first bottle mouth is detachably connected with a first bottle cap, and the second bottle mouth is detachably connected with a second bottle cap.
Further, the second bottle opening is obliquely upwards arranged, and an included angle between the second bottle opening and the bottle body is 45-60 degrees.
Further, the upper end of the stirring rod can be rotatably arranged on the top of the bottle body in a penetrating way, and an air inlet duct is formed in the axial center position of the upper part of the stirring rod;
a plurality of ventilation holes are formed in the peripheral wall of the middle upper part of the stirring rod at intervals, and the ventilation holes are positioned in the corresponding culture cavity and communicated with the air inlet channels.
Further, a plurality of auxiliary rods are arranged on the peripheral wall of the middle lower part of the stirring rod at intervals, and the top end of the stirring rod is detachably connected with an air inlet cover through threads.
Further, a through hole is formed in the center of the top of the end cover, and the top of the end cover is rotatably connected with the top of the bottle body; the top end of the stirring rod passes through the through hole and is upwards arranged.
Further, the inner diameter of the first hole is larger than the inner diameter of the first bottle mouth; the inner diameter of the second hole is larger than the inner diameter of the second bottle opening.
Further, the inner peripheral wall of end cover bottom is provided with annular spacing protruding, spacing protruding with set up in spacing recess sliding connection of bottle lateral wall.
Further, four first marks are arranged on the side wall of the bottle body at intervals, and a second mark is arranged on the side wall of the middle lower part of the end cover.
Further, a first connecting piece is arranged on the side wall of the bottle body, and a second connecting piece detachably connected with the first connecting piece is arranged on the side wall of the end cover.
Compared with the prior art, the utility model has the following technical effects:
(1) According to the multi-port cell culture bottle capable of rapidly sampling, the end cover is connected with the bottle body in a sliding manner, so that different positions of different culture cavities are sampled, the number of bottle openings is reduced, and the bottle is convenient to take;
(2) According to the multi-port cell culture bottle capable of sampling rapidly, the inner cavity of the bottle body is uniformly divided into four culture cavities through the cross-shaped partition plate, so that four different cells can be cultured in the bottle body at the same time, and sampling detection and comparison of different cells are facilitated;
(3) According to the multi-port cell culture bottle capable of sampling rapidly, the stirring rod is rotated to stir the culture solution in the corresponding culture cavity, shaking is not needed, the operation is simple and convenient, and the ventilation of the interior of the culture cavity is realized by arranging the air inlet duct at the axial center position of the upper part of the stirring rod.
Drawings
FIG. 1 is a schematic diagram showing a three-dimensional structure of a multi-port cell culture flask for rapid sampling according to the present utility model;
FIG. 2 is a schematic diagram showing a three-dimensional structure of a multi-port cell culture flask for rapid sampling according to the present utility model;
FIG. 3 is a schematic view (I) of a multi-port cell culture flask with rapid sampling in partial cross-section according to the present utility model;
FIG. 4 is a schematic partial sectional perspective view of a multi-port cell culture flask for rapid sampling according to the present utility model (II);
FIG. 5 is a schematic view showing a partial cross-sectional structure of a multi-port cell culture flask for rapid sampling according to the present utility model;
FIG. 6 is a schematic view showing a partial perspective view of a multi-port cell culture flask for rapid sampling according to the present utility model;
FIG. 7 is a schematic cross-sectional view of a rapid sampling multi-port cell culture flask according to the present utility model in a top view;
FIG. 8 is a schematic view showing a partial cross-sectional structure of a stirring rod in a multi-port cell culture flask for rapid sampling according to the present utility model;
FIG. 9 is an enlarged schematic view at A in FIG. 8;
FIG. 10 is a schematic view of a multi-port cell culture flask with rapid sampling in a partially sectioned perspective view (III) according to the present utility model.
Wherein the reference numerals are as follows:
1. a bottle body; 2. a cross-shaped partition plate; 3. a culture chamber; 4. a stirring rod; 5. a first hole; 6. a second hole; 7. an end cap; 8. a first bottle mouth; 9. a second bottle mouth; 10. a first bottle cap; 11. a second bottle cap; 12. an air inlet duct; 13. ventilation holes; 14. an auxiliary lever; 15. an air inlet cover; 16. a limit protrusion; 17. a limit groove; 18. a first identifier; 19. a second identifier; 20. a first connector; 21. and a second connecting piece.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It should be noted that, without conflict, the embodiments of the present utility model and features of the embodiments may be combined with each other.
The utility model is further described below with reference to the drawings and specific examples, which are not intended to be limiting.
A multi-port cell culture flask for rapid sampling is shown in fig. 1-7, and comprises a flask body 1, a cross-shaped partition plate 2 and four stirring rods 4.
The cross-shaped partition board 2 is arranged in the bottle body 1 to divide the inner cavity of the bottle body 1 into four independent culture cavities 3 uniformly, and each culture cavity 3 is correspondingly provided with a stirring rod 4; four first holes 5 are formed in the top of the bottle body 1 at intervals, and four second holes 6 are formed in the middle upper portion of the side wall of the bottle body at intervals; the top end of the bottle body 1 is covered with an end cover 7, and the inner wall of the end cover 7 is in sliding connection with the side wall of the bottle body 1; and the top of the end cover 7 is provided with a first bottle mouth 8 corresponding to the first hole 5, and the side wall of the end cover 7 is provided with a second bottle mouth 9 corresponding to the second hole 6.
Specifically, the four first holes 5 and the four second holes 6 are symmetrically arranged by taking the central axis of the bottle body 1 as a symmetry axis; a first hole 5 and a second hole 6 are arranged in each culture cavity 3; by rotating the end cap 7, the first hole 5 in the same culture cavity 3 is communicated with the first bottle opening 8, and the second hole 6 is communicated with the second bottle opening 9.
The inner cavity of the bottle body 1 is uniformly divided into four culture cavities 3 through the cross-shaped partition plates 2, so that four different cells can be cultured in the bottle body 1 at the same time, and sampling, detection and comparison of the different cells are facilitated; the end cover 7 is rotated to enable the first bottle opening 8 and the second bottle opening 9 to be respectively communicated with the first hole 5 and the second hole 6, so that sampling of different positions of the corresponding culture cavity 3 is facilitated; the number of bottle openings on the end cover 7 is reduced through sliding connection between the end cover 7 and the bottle body 1, so that the bottle is convenient to take; the stirring rod 4 is rotated to stir the culture solution in the corresponding culture cavity 3, so that shaking is not needed, and the operation is simple and convenient.
In some of these embodiments, the central axis of the first bottle mouth 8 is disposed on the same plane as the central axis of the second bottle mouth 9.
In some of these embodiments, the bottom end of the stirring rod 4 is rotatably connected to a bottom groove of the bottle body 1.
In some embodiments, the four stirring rods 4 are symmetrically arranged with the central axis of the bottle body 1 as a symmetry axis.
In some of these embodiments, the stirring rod 4 is near the central axis of the bottle 1 and the first hole 5 is far from the central axis of the bottle 1.
Further, as shown in fig. 1 and 3 to 4, a first bottle cap 10 is detachably connected to the first bottle mouth 8, and a second bottle cap 11 is detachably connected to the second bottle mouth 9.
In some of these embodiments, the first neck finish 8 is removably threaded to the first bottle cap 10 and the second neck finish 9 is removably threaded to the second bottle cap 11.
Further, as shown in fig. 1 to 5, the second bottle mouth 9 is arranged obliquely upwards, and the included angle between the second bottle mouth 9 and the bottle body 1 is 45 degrees to 60 degrees.
Further, the included angle between the second bottle mouth 9 and the bottle body 1 is 45-50 degrees.
In some of these embodiments, the angle between the second mouth 9 and the bottle body 1 is 45 °.
Further, as shown in fig. 5 to 10, the upper end of the stirring rod 4 is rotatably arranged on the top of the bottle body 1, and an air inlet duct 12 is arranged at the axial center position of the upper part; a plurality of ventilation holes 13 are arranged on the peripheral wall of the middle upper part of the stirring rod 4 at intervals, and the ventilation holes 13 are positioned in the corresponding culture cavity 3 and are communicated with the air inlet pore canal 12.
Specifically, air flows from the air inlet duct 12 to the air holes 13 and is discharged into the culture chamber 3 through the air holes 13.
Further, as shown in fig. 5 to 10, a plurality of auxiliary rods 14 are provided at intervals on the outer peripheral wall of the middle lower part of the stirring rod 4, and an air inlet cover 15 is detachably screwed on the top end of the stirring rod 4.
In some of these embodiments, the culture solution in the culture chamber 3 is sufficiently stirred by rotating the tip of the stirring rod 4 to rotate the plurality of auxiliary rods 14.
Further, as shown in fig. 1 to 4, in order to avoid the stirring rod 4 from obstructing the rotation of the end cover 7, a through hole is formed in the center of the top of the end cover 7, and the top of the end cover 7 is rotatably connected with the top of the bottle body 1; the top end of the stirring rod 4 passes through the through hole and is upwards arranged.
Further, as shown in fig. 3 and 5, in order to facilitate sampling through the first bottle mouth 8 and the second bottle mouth 9, the inner diameter of the first hole 5 is larger than the inner diameter of the first bottle mouth 8; the inner diameter of the second bore 6 is larger than the inner diameter of the second bottle mouth 9.
Further, as shown in fig. 1 to 3 and 6, in order to prevent the end cap 7 from separating from the bottle body 1, an annular limiting protrusion 16 is provided on the inner peripheral wall of the bottom end of the end cap 7, and the limiting protrusion 16 is slidably connected with a limiting groove 17 provided on the outer side wall of the bottle body 1.
Further, as shown in fig. 3 to 5, four first marks 18 are provided on the side wall of the bottle body 1 at intervals, and a second mark 19 is provided on the middle lower side wall of the end cap 7.
In some of these embodiments, the first and second mouths 8, 9 are brought into communication with the first and second apertures 5, 6, respectively, by aligning the second indicia 19 with the first indicia 18.
Further, as shown in fig. 2, in order to avoid unnecessary rotation of the cap 7, a first connector 20 is provided on a sidewall of the bottle body 1, and a second connector 21 detachably connected to the first connector 20 is provided on a sidewall of the cap 7.
In some embodiments, the first connector 20 and the second connector 21 are velcro.
In some of these embodiments, the second connector 21 is located below the limit groove 17.
The application method of the utility model is as follows:
during sampling, the end cover 7 is rotated so that the first mark 18 is aligned with the second mark 19, namely the first bottle opening 8 and the second bottle opening 9 are respectively communicated with the first hole 5 and the second hole 6;
the first bottle cap 10 and the second bottle cap 11 are respectively opened to sample different positions of the corresponding culture cavity 3;
after the sampling is finished, the first bottle cap 10 and the second bottle cap 11 are detachably connected to the first bottle opening 8 and the second bottle opening 9 respectively;
the end cap 7 is turned to detachably connect the first connector 20 with the second connector 21.
During stirring, the stirring rod 4 is rotated so that the corresponding auxiliary rods 14 stir the culture solution in the corresponding culture chambers 3.
When ventilation is performed, the air inlet cover 15 is opened, air flows from the air inlet pore canal 12 to the ventilation holes 13, and then is discharged into the corresponding culture cavity 3 through the ventilation holes 13.
The foregoing description is only illustrative of the preferred embodiments of the present utility model and is not to be construed as limiting the scope of the utility model, and it will be appreciated by those skilled in the art that equivalent substitutions and obvious variations may be made using the description and illustrations of the present utility model, and are intended to be included within the scope of the present utility model.
Claims (10)
1. The utility model provides a many mouthfuls of type cell culture bottle of quick sample, its characterized in that includes bottle (1), cross baffle (2) and four stirring rod (4), wherein:
the cross-shaped partition plate (2) is arranged in the bottle body (1) so as to divide the inner cavity of the bottle body (1) into four independent culture cavities (3) uniformly, and each culture cavity (3) is internally provided with a stirring rod (4) correspondingly;
four first holes (5) are formed in the top of the bottle body (1) at intervals, and four second holes (6) are formed in the middle upper portion of the side wall of the bottle body at intervals; an end cover (7) is arranged on the top end cover of the bottle body (1), and the inner wall of the end cover (7) is in sliding connection with the side wall of the bottle body (1); and the top of the end cover (7) is provided with a first bottleneck (8) corresponding to the first hole (5), and the side wall of the end cover (7) is provided with a second bottleneck (9) corresponding to the second hole (6).
2. The rapid sampling multi-port cell culture flask according to claim 1, wherein a first bottle cap (10) is detachably connected to the first bottle mouth (8), and a second bottle cap (11) is detachably connected to the second bottle mouth (9).
3. The rapid sampling multi-port cell culture flask according to claim 1, wherein the second flask mouth (9) is arranged obliquely upwards, and an included angle between the second flask mouth (9) and the flask body (1) is 45-60 °.
4. The rapid sampling multiport cell culture flask according to claim 1, wherein the upper end of the stirring rod (4) is rotatably arranged on the top of the flask body (1), and an air inlet duct (12) is arranged at the axial center of the upper part;
a plurality of ventilation holes (13) are formed in the peripheral wall of the upper middle part of the stirring rod (4) at intervals, and the ventilation holes (13) are positioned in the corresponding culture cavity (3) and are communicated with the air inlet pore canal (12).
5. The rapid sampling multiport cell culture flask according to claim 1, wherein a plurality of auxiliary rods (14) are arranged on the peripheral wall of the middle lower part of the stirring rod (4) at intervals, and an air inlet cover (15) is detachably connected with the top end of the stirring rod (4) through threads.
6. The rapid sampling multi-port cell culture flask according to claim 1, wherein a through hole is formed in the center of the top of the end cover (7), and the top of the end cover (7) is rotatably connected with the top of the flask body (1); the top end of the stirring rod (4) passes through the through hole and is upwards arranged.
7. The rapid sampling multi-port cell culture flask according to claim 1, wherein the inner diameter of the first hole (5) is larger than the inner diameter of the first bottle mouth (8); the inner diameter of the second hole (6) is larger than the inner diameter of the second bottle opening (9).
8. The rapid sampling multi-port cell culture flask according to claim 1, wherein an annular limiting protrusion (16) is arranged on the inner peripheral wall of the bottom end of the end cover (7), and the limiting protrusion (16) is slidably connected with a limiting groove (17) arranged on the outer side wall of the flask body (1).
9. The rapid sampling multi-port cell culture flask according to claim 1, wherein four first markers (18) are arranged on the side wall of the flask body (1) at intervals, and a second marker (19) is arranged on the side wall of the middle lower portion of the end cover (7).
10. The rapid sampling multi-port cell culture flask according to claim 1, wherein a first connector (20) is provided on the side wall of the flask body (1), and a second connector (21) detachably connected to the first connector (20) is provided on the side wall of the end cap (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321627159.7U CN220056878U (en) | 2023-06-26 | 2023-06-26 | Multi-port cell culture bottle capable of sampling rapidly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321627159.7U CN220056878U (en) | 2023-06-26 | 2023-06-26 | Multi-port cell culture bottle capable of sampling rapidly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220056878U true CN220056878U (en) | 2023-11-21 |
Family
ID=88785655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321627159.7U Active CN220056878U (en) | 2023-06-26 | 2023-06-26 | Multi-port cell culture bottle capable of sampling rapidly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220056878U (en) |
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2023
- 2023-06-26 CN CN202321627159.7U patent/CN220056878U/en active Active
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