CN219194958U - Fermentation cylinder sampling device - Google Patents

Abstract

The utility model relates to the technical field of biological fermentation and discloses a fermentation tank sampling device, which comprises a fermentation tank body and further comprises a sampling assembly, wherein the sampling assembly comprises a sampling pipe which is partially positioned in the fermentation tank and a sampling pipe which is positioned outside the fermentation tank, one end of the sampling pipe, which is far away from the fermentation tank, is connected with a first sampling valve, one end of the first sampling valve, which is far away from the sampling pipe, is connected with a four-way pipe, the other three ends of the four-way pipe are respectively connected with a second sampling valve, a third sampling valve and a fourth sampling valve, one end of the second sampling valve, which is far away from the four-way pipe, is connected with a first pipeline, compressed air is introduced into the sampling pipe, and one end of the fourth sampling valve, which is far away from the four-way pipe, is connected with a second sampling valve, a third sampling valve and a fourth sampling valve, which is far away from the four-way pipe, is connected with a first pipeline, and saturated steam is introduced into the sampling pipe. The utility model has the following advantages and effects: by adding the compressed air inlet, the sampling pipeline can maintain positive pressure for a long time, so that the invasion of miscellaneous bacteria can be effectively prevented; and steam is not required to be introduced for a long time, so that the energy consumption is effectively reduced.

Description

Fermentation cylinder sampling device

Technical Field

The utility model relates to the technical field of biological fermentation, in particular to a fermentation tank sampling device.

Background

The fermentation tank is a biological reaction container for culturing microorganisms or cells, and in the fermentation process, in order to timely grasp the fermentation condition of fermentation liquid in the fermentation tank, the temperature dissolved oxygen, the PH value and the pressure in the fermentation tank are controlled, and a sterile sample is taken for sterile tracking inspection, so that a certain fermentation liquid needs to be taken out through a sampling device of the fermentation tank to carry out assay analysis. At present, a sampling device for a fermentation tank mainly comprises a sampling tube, a sampling valve and a sampling tube, when sampling is carried out, as fermentation liquor in the fermentation tank continuously reacts, gas volume can be generated in the tank, the sampling tube is always negative pressure, the fermentation liquor before the sampling tube always remains, and the fermentation liquor obtained by sampling can not react with the real condition of the fermentation liquor in the fermentation tank at the moment, so that a new sampling device is needed to solve the problems.

Disclosure of Invention

The utility model aims to provide a fermentation tank sampling device which has the advantages of convenience in use, good sampling effect and energy consumption reduction.

The technical aim of the utility model is realized by the following technical scheme: the utility model provides a fermentation cylinder sampling device, includes the fermentation cylinder body, still includes the sampling assembly, the sampling assembly is including the part is located the inside sampling tube of fermentation cylinder and be located the outer play sampling tube of fermentation cylinder, the sampling tube is kept away from the one end of fermentation cylinder and is connected with first sample valve, the one end that the sampling tube was kept away from to first sample valve is connected with the four-way pipe, second sample valve, third sample valve and fourth sample valve are connected respectively to other three ends of four-way pipe, the one end that the four-way pipe was kept away from to the second sample valve meets with play sampling tube, the one end that the four-way pipe was kept away from to the third sample valve is connected with pipeline one, the one end that the four-way pipe was kept away from to the fourth sample valve is connected with pipeline two.

By adopting the technical scheme, when the device is used, the first, second and third sampling valves are closed, the fourth sampling valve is opened first, saturated steam is introduced, and then the second sampling valve is slightly opened to discharge steam and condensed water for disinfection; after disinfection is completed, the second sampling valve and the fourth sampling valve are sequentially closed, the third sampling valve is opened, sterile air is introduced, the first sampling valve is opened again, sterile compressed air is used for purging the sampling pipeline, after purging for 5 minutes, the third sampling valve is slowly tightened, the slightly opened state is kept, the second sampling valve is slowly opened at the moment, fermentation liquor in the fermentation tank can enter the sampling pipeline at the moment, and when the fermentation liquor flows out from a sample outlet of the sample outlet pipe, the second sampling valve is fully opened, so that sampling is completed.

The utility model is further provided with: the sampling tube is connected to the bottom of the fermentation tank from the top of the fermentation tank.

By adopting the technical scheme, the sampling tube is connected from the top of the fermentation tank, so that the fragile tank structure at the bottom of the fermentation tank can not be damaged.

The utility model is further provided with: the first sampling valve and the second sampling valve are DN15 manual diaphragm valves.

By adopting the technical scheme, the closing element of the diaphragm valve is not wetted by the process medium, the flowing material flow passes through linearly or approximately linearly, and small pressure drop is generated, so that turbulent flow is avoided; the diaphragm valve may also be throttled.

The utility model is further provided with: the inner diameters of the sampling tube, the sampling tube and the four-way tube are all 15mm.

By adopting the technical scheme, if the inner diameter of the sampling tube is too large in the fermentation tank, the flow is increased, more fermentation liquor needs to be discharged, waste is caused, air and tail gas are entrained in the fermentation liquor, foam is more during sampling, the flow is large, the fermentation liquor is easy to splash, and the thallus splashed by the sampling port pollutes the surrounding environment; when taking sterile samples, the inner diameter of the sample outlet pipe is large, the opening of the sampling bottle is small, fermentation liquor is easy to be stained with the opening of the sterile sampling bottle, so that sterile sampling pollution is caused, and the sterile tracking false bacteria infection condition occurs.

The utility model is further provided with: the sampling port of the sampling tube is consistent with the direction of the sampling outlet of the sampling tube.

By adopting the technical scheme, the sampling port of the sampling tube is positioned in the fermentation broth and is vertically arranged downwards, and when sampling is performed, the fermentation broth is extruded downwards by the air pressure generated in the fermentation tank, so that the fermentation broth enters the sampling tube from the sampling port; the vertical downward setting of appearance mouth of sampling tube makes the zymotic fluid flow out from the appearance mouth in can directly getting into the sample bottle through the action of gravity, prevents that the zymotic fluid from splashing.

The utility model is further provided with: from the first conduit is compressed sterile air.

Through adopting above-mentioned technical scheme, through inserting compressed air, make sampling tube keep positive pressure for a long time, can prevent effectively that miscellaneous fungus from invading, the compressed air of letting in is originally the fermentation needs simultaneously.

The utility model is further provided with: saturated steam is fed from the pipe.

By adopting the technical scheme, the saturated steam is used for disinfecting the first sampling valve to the sampling pipe, so that the sampling liquid is prevented from being polluted by mixed bacteria.

The beneficial effects of the utility model are as follows:

1. when the device is used, the first, second and third sampling valves are closed, the fourth sampling valve is opened first, saturated steam is introduced, and then the second sampling valve is slightly opened to discharge steam and condensed water for disinfection; after disinfection is completed, the second sampling valve and the fourth sampling valve are sequentially closed, the third sampling valve is opened, sterile air is introduced, the first sampling valve is opened again, sterile compressed air is used for purging the sampling pipeline, after purging for 5 minutes, the third sampling valve is slowly tightened, the slightly opened state is kept, the second sampling valve is slowly opened at the moment, fermentation liquor in the fermentation tank can enter the sampling pipeline at the moment, and when the fermentation liquor flows out from a sample outlet of the sample outlet pipe, the second sampling valve is fully opened, so that sampling is completed.

2. The utility model adds a compressed air inlet, but does not need to maintain steam to continuously sterilize the sampling port; and the sampling port is directly connected into the fermentation tank from the bottom, so that the structure of the bottom of the tank body is extremely easy to damage in long-term use.

3. By accessing compressed air, the sampling pipeline can maintain positive pressure for a long time, and the invasion of miscellaneous bacteria can be effectively prevented. Moreover, steam is not required to be introduced for a long time, so that the energy consumption is effectively reduced, and the introduced compressed air is needed for fermentation.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present utility model, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the present utility model.

In the figure, 1, a fermentation tank; 2. a sampling assembly; 3. a sampling tube; 31. a material taking port; 4. a sampling tube; 41. a sample outlet; 5. a first sampling valve; 6. a second sampling valve; 7. a third sampling valve; 8. a fourth sampling valve; 9. a four-way pipe; 10. a first pipeline; 11. and a second pipeline.

Detailed Description

The technical scheme of the present utility model will be clearly and completely described in connection with specific embodiments. It will be apparent that the described embodiments are only some, but not all, embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to fall within the scope of the utility model.

Examples: please refer to fig. 1, a fermentation tank sampling device, including fermentation tank 1 body, still include sampling assembly 2, sampling assembly 2 is including part being located the inside sampling tube 3 of fermentation tank 1 and being located the outer play sampling tube 4 of fermentation tank 1, the one end that fermentation tank 1 was kept away from to sampling tube 3 is connected with first sampling valve 5, the one end that sampling tube 3 was kept away from to first sampling valve 5 is connected with four-way pipe 9, second sampling valve 6 is connected respectively to other three ends of four-way pipe 9, third sampling valve 7 and fourth sampling valve 8, the one end that four-way pipe 9 was kept away from to second sampling valve 6 meets with play sampling tube 4, the one end that four-way pipe 9 was kept away from to third sampling valve 7 is connected with pipeline one 10, the one end that four-way pipe 9 was kept away from to fourth sampling valve 8 is connected with pipeline two 11, let in compressed sterile air from pipeline two 11 lets in saturated steam.

By introducing compressed air, the positive pressure of the 3 channels of the sampling tube is maintained for a long time, so that the invasion of mixed bacteria can be effectively prevented, and meanwhile, the introduced compressed air is needed by fermentation; the saturated steam is used for disinfecting the first sampling valve 5 to the sampling pipe 4 and preventing the sampling liquid from being polluted by mixed bacteria.

As shown in fig. 1, a sampling tube 3 is connected from the top of a fermentation tank 1 to the bottom of the fermentation tank 1; the first sampling valve 5 and the second sampling valve 6 are DN15 manual diaphragm valves, and the inner diameters of the sampling tube 3, the sampling tube 4 and the four-way tube 9 are 15mm; the sampling port of the sampling tube 3 is consistent with the direction of the sampling port 41 of the sampling tube 4.

The sampling tube 3 is connected from the top of the fermentation tank 1, so that the fragile tank structure at the bottom of the fermentation tank 1 is not damaged.

The closing element of the diaphragm valve is not wetted by the process medium, the flow stream passes straight or approximately straight, and a small pressure drop is generated, so that turbulence is avoided; the diaphragm valve may also be throttled.

If the inner diameter of the sampling tube 3 in the fermentation tank 1 is too large, the flow rate is increased, more fermentation liquid needs to be discharged, waste is caused, air and tail gas are entrained in the fermentation liquid, foam is more during sampling, the flow rate is large, the fermentation liquid is easy to splash, and the splashed thalli at the sampling port pollute the surrounding environment; when taking aseptic samples, the inner diameter of the sampling tube 4 is large, the opening of the sampling bottle is small, fermentation liquor is easy to be stained with the opening of the aseptic sampling bottle, aseptic sampling pollution is caused, and aseptic tracking false bacteria infection occurs.

The sampling port of the sampling tube 3 is positioned in the fermentation liquor and is vertically arranged downwards, and when sampling is performed, the fermentation liquor is extruded downwards by the air pressure generated in the fermentation tank 1, so that the fermentation liquor enters the sampling tube 3 from the sampling port; the sample outlet 41 of the sample outlet pipe 4 is vertically downwards arranged, so that fermentation liquid can directly enter the sampling bottle under the action of gravity when flowing out from the sample outlet 41, and the fermentation liquid is prevented from splashing.

Sampling flow: when the device is used, the first, second and third sampling valves 7 are closed, the fourth sampling valve 8 is opened firstly, saturated steam is introduced, and then the second sampling valve 6 is slightly opened to discharge steam and condensed water for disinfection; after disinfection is completed, the second sampling valve 6 and the fourth sampling valve 8 are sequentially closed, the third sampling valve 7 is opened, sterile air is introduced, the first sampling valve 5 is opened again, the sterile compressed air is used for purging the 3 channels of the sampling tube, after purging for 5 minutes, the third sampling valve 7 is slowly tightened, the slightly opened state is kept, the second sampling valve 6 is slowly opened at the moment, fermentation liquor in the fermentation tank 1 can enter the 3 channels of the sampling tube at the moment, and when the fermentation liquor flows out from the sample outlet 41 of the sample outlet tube 4, the second sampling valve 6 is fully opened, so that sampling is completed.

Claims (7)

1. The utility model provides a fermentation cylinder sampling device, includes fermentation cylinder (1) body, its characterized in that: still include sample subassembly (2), sample subassembly (2) including part be located inside sampling tube (3) of fermentation cylinder (1) and be located outer play sampling tube (4) of fermentation cylinder (1), the one end that fermentation cylinder (1) was kept away from to sampling tube (3) is connected with first sample valve (5), the one end that sampling tube (3) was kept away from to first sample valve (5) is connected with four-way pipe (9), second sample valve (6), third sample valve (7) and fourth sample valve (8) are connected respectively to other three ends of four-way pipe (9), the one end that four-way pipe (9) was kept away from to second sample valve (6) meets with play sampling tube (4), the one end that four-way pipe (9) was kept away from to third sample valve (7) is connected with pipeline one (10), the one end that four-way pipe (9) was kept away from to fourth sample valve (8) is connected with pipeline two (11).

2. A fermenter sampling apparatus according to claim 1, wherein: the sampling tube (3) is connected to the bottom of the fermentation tank (1) from the top of the fermentation tank (1).

3. A fermenter sampling apparatus according to claim 1, wherein: the first sampling valve (5) and the second sampling valve (6) are DN15 manual diaphragm valves.

4. A fermenter sampling apparatus according to claim 1, wherein: the inner diameters of the sampling tube (3), the sampling tube (4) and the four-way tube (9) are all 15mm.

5. A fermenter sampling apparatus according to claim 1, wherein: the sampling port of the sampling tube (3) is consistent with the direction of the sampling port (41) of the sampling tube (4).

6. A fermenter sampling apparatus according to claim 1, wherein: from the first conduit (10) is compressed sterile air.

7. A fermenter sampling apparatus according to claim 1, wherein: saturated steam is introduced from the second pipeline (11).

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