CN217809428U - Biological fermentation tank convenient to sample - Google Patents
Biological fermentation tank convenient to sample Download PDFInfo
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- CN217809428U CN217809428U CN202222533622.3U CN202222533622U CN217809428U CN 217809428 U CN217809428 U CN 217809428U CN 202222533622 U CN202222533622 U CN 202222533622U CN 217809428 U CN217809428 U CN 217809428U
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Abstract
The utility model relates to a biological fermentation tank convenient for sampling, which comprises a pipe cap, a cylinder body and an equipment flange, wherein the pipe cap is connected at the lower end of the cylinder body, the equipment flange is connected at the upper end of the cylinder body, an integral jacket is sleeved outside the cylinder body, a heat-insulating jacket is sleeved outside the integral jacket, a sampling device is connected at the lower side surface of the cylinder body through a sampling pipeline, and a spraying device is connected at the upper side surface of the cylinder body; the sampling pipe passes whole cover and heat preservation cover, and the inner chamber intercommunication of sampling pipe and barrel, sampling device include the sample valve, and the sample valve includes 2 at least intercommunication mouths and 1 inlet, and the inlet is connected with the sampling pipe. The beneficial effects of the utility model are that: can be through the direct leading-in sample valve of liquid in with fermentation tank of gravity, the intercommunication mouth through the sample valve can be convenient carry the sample to detect in the check out test set of difference to disinfect the sample through high temperature steam simultaneously, reduce the probability that the sample received the pollution, improve the accuracy that the sample detected.
Description
Technical Field
The utility model relates to a biological fermentation technical field, specifically a fermentation tank convenient to sample.
Background
The fermentation of microbial strains requires the addition of various culture media, strains and water, and the microbial strains are mixed uniformly by proper stirring under certain temperature and concentration conditions so as to be fermented fully. The fermentation tank is culture equipment of strains for bioengineering, the fermentation process is an aseptic and pollution-free process, and the fermentation tank adopts an aseptic system, so that the pollution of microorganisms in the air is avoided and prevented. Typical fermenters are classified as solid fermenters and liquid fermenters. The fermentation tank is divided into a seed tank (seed fermentation tank) and a fermentation tank according to the volume and the application. The fermentation tank is classified into a vented fermentation tank and an anaerobic fermentation tank according to the aerobic type. In order to guarantee that the convenience is judged the condition of fermentation, generally can adopt the liquid that sets up in the pipeline extraction fermentation cylinder at fermentation tank top to take a sample, need set up air exhaust device or negative pressure pipeline alone.
A sterile sampling device of a microbial fermentation tank as disclosed in CN202121965539.2, which comprises a sterile sampling system and a sterile sampling bottle; the sterile sampling system consists of a steam inlet valve, a first sampling valve, a second sampling valve, a third sampling valve, a steam pipe, a sample outlet pipe and a first sampling chuck; aseptic sampling bottle constitute by second sample chuck, exhaust valve, fourth sample valve, sample pipeline, sampling bottle, bottle lid, blast pipe and breathing filter. The sampling process is complicated to because the sample of single nozzle, inconvenient to carry out simultaneous processing to the sample.
Disclosure of Invention
In order to overcome at least partial defect among the prior art, the embodiment of the utility model provides a fermentation tank convenient to sample, simple structure, convenient to use can carry out the sample and the detection of a plurality of samples simultaneously, reduces the number of times of cutting apart to the sample, reduces the probability that the sample received the pollution.
The utility model relates to a biological fermentation tank convenient for sampling, which comprises a pipe cap, a cylinder body and an equipment flange, wherein the pipe cap is connected with the lower end of the cylinder body, the equipment flange is connected with the upper end of the cylinder body, an integral clamping sleeve is sleeved on the outer side of the cylinder body, a heat-insulating clamping sleeve is sleeved on the outer side of the integral clamping sleeve, the lower side surface of the cylinder body is connected with a sampling device through a sampling pipeline, and the upper side surface of the cylinder body is connected with a spraying device;
the sampling pipeline penetrates through the integral jacket and the heat-preservation jacket, the sampling pipeline is communicated with the inner cavity of the cylinder, the sampling device comprises a sampling valve, the sampling valve comprises at least 2 communication ports and 1 liquid inlet, the liquid inlet is connected with the sampling pipeline, and one communication port is connected with the steam disinfection pipeline;
spray set includes return bend, connector and sprays the ball, the return bend passes out near the one end of import the lateral wall of barrel, the detachable plug core that is connected with in import of return bend, spray the ball pass through the connector with the return bend is connected, a plurality of sprays the mouth has been seted up to the downside that sprays the ball, the connector has the lower concave part of undercut the row's of liquid that is used for the row to go to the utmost return bend and/or sprays in the ball is seted up to the downside of lower concave part is as far as possible mouthful.
Further, the downside of pipe cap is connected with the landing leg, the quantity of landing leg is 2, 2 the landing leg is followed the axis of pipe cap sets up for the axial symmetry, 2 be provided with tank bottoms valves between the landing leg, tank bottoms valves through the discharge pipe with the inner chamber intercommunication of barrel.
Furthermore, the tank bottom valve group comprises at least 2 valve bodies connected with the discharge pipeline, and an included angle of 60-120 degrees is formed between central axes of liquid outlets of the two valve bodies.
Furthermore, the bottom of the inner cavity of the pipe cap is connected with an anti-vortex plate, the bottom of the anti-vortex plate protrudes downwards to form a protruding part, the protruding part is connected with the bottom of the pipe cap, and the discharge pipeline is positioned on the lower side of the anti-vortex plate.
Further, the mounting panel is connected to equipment flange upper end, the mounting panel is connected with rabbling mechanism, the rabbling mechanism lower extreme is connected with the (mixing) shaft, the (mixing) shaft stretches into the part of the inner chamber of barrel is connected with the stirring rake.
Furthermore, the downside of stirring rake is connected with stirring baffle, stirring baffle with the inner wall of barrel or the inner wall connection of pipe cap.
Further, the side of the cylinder is connected with an observation window for observing the fermentation condition in the cylinder.
The utility model discloses an useful part lies in: the liquid in the biological fermentation tank can be directly guided into the sampling valve through gravity, the sample can be conveniently conveyed into the detection equipment for simultaneous detection through the communication port of the sampling valve, and the sample is disinfected through high-temperature steam, so that the pollution probability of the sample is reduced, and the accuracy of the sample detection is improved;
spray ball bottom and be 360 and seted up the mouth that sprays, washing barrel inner wall that can be better (including paddle shade region), in order to prevent that remaining washing liquid from causing the destruction to the fermentation environment among the fermentation process, arrange the mouth through the return bend that arranges to the greatest extent and come to arrange the washing liquid that sprays in the ball smoothly to the greatest extent, reduce the washing liquid as far as possible and remain.
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of a biological fermenter for facilitating sampling.
Fig. 2 is a schematic view of the installation position of the shower device.
Fig. 3 is a schematic structural view of the spray device.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts all belong to the protection scope of the present invention.
Referring to fig. 1, fig. 2 and fig. 3, in a preferred embodiment of the present invention, a biological fermentation tank convenient for sampling comprises a pipe cap 1, a cylinder 2 and an equipment flange 3, wherein the pipe cap 1 is connected to the lower end of the cylinder 2, the equipment flange 3 is connected to the upper end of the cylinder 2, an integral jacket 4 is sleeved outside the cylinder 2, an insulation jacket 5 is sleeved outside the integral jacket 4, a sampling device 7 is connected to the lower side surface of the cylinder 2 through a sampling pipe 6, and a spraying device 8 is connected to the upper side surface of the cylinder 2;
the sampling pipeline 6 penetrates through the integral jacket 4 and the heat-insulating jacket 5, the sampling pipeline 6 is communicated with the inner cavity of the cylinder 2, the sampling device 7 comprises a sampling valve 71, the sampling valve 71 comprises at least 2 communication ports 711 and 1 liquid inlet 712, the liquid inlet 712 is connected with the sampling pipeline 6, and one of the communication ports 711 is connected with a steam disinfection pipeline. Sampling tube 6 connects the downside at barrel 2, and then can be through the direct leading-in sampling valve 71 of liquid with in the fermentation tank of gravity, can be convenient carry the sample to detect in the check out test set through the intercommunication mouth of sampling valve 71 to disinfect the sample through high temperature steam simultaneously, reduce the probability that the sample received the pollution, improve the accuracy that the sample detected. In the practical implementation process, in order to obtain a better sterilization effect, high-temperature steam with the temperature of 121 ℃ is introduced into the steam sterilization pipeline to sterilize the sampled sample, and the inside of the cavity can be sterilized before the culture medium is introduced.
In the above embodiment, in order to effectively support the cylinder body 2, the lower side of the pipe cap 1 is connected with the support legs 9, the number of the support legs 9 is 2, 2 the support legs 9 are arranged along the central axis of the pipe cap 1 in an axisymmetric manner, 2 tank bottom valve groups 10 are arranged between the support legs 9, and the tank bottom valve groups 10 are communicated with the inner cavity of the cylinder body 2 through a discharge pipeline.
In the above embodiment, the tank bottom valve group 10 includes at least 2 valve bodies 101 connected to the discharge pipe, and an included angle of 60 ° to 120 ° is formed between central axes of liquid outlets of the two valve bodies 101. In actual implementation, two valve bodies 101 are connected with different storage jar respectively, can carry the liquid that the fermentation was accomplished and the liquid that the fermentation failed to different storage jar in, reduce the setting degree of difficulty of pipeline.
In the above embodiment, the bottom of the inner cavity of the tube cap 1 is connected with the anti-vortex plate 11, the bottom of the anti-vortex plate 11 protrudes downwards to form a protruding portion, the protruding portion is connected with the bottom of the tube cap 1, and the discharge pipeline is located on the lower side of the anti-vortex plate 11. In practical implementation, in order to obtain a better anti-vortex effect, the anti-vortex plate 11 is a cross-shaped plate.
In the above embodiment, in order to mix the culture medium, the strains and the water in the biological fermentation tank uniformly, the upper end of the equipment flange is connected with the mounting plate 12, the mounting plate 12 is connected with the stirring mechanism 13, the lower end of the stirring mechanism 13 is connected with the stirring shaft 131, and the part of the stirring shaft 131 extending into the inner cavity of the cylinder body is connected with the stirring paddle 132.
In the above embodiment, a stirring baffle (not shown) is connected to the lower side of the stirring paddle 132, and the stirring baffle is connected to the inner wall of the cylinder 2 or the inner wall of the cap 1. The stirring baffles can change the rotational tangential flow to radial and axial flow. It can also greatly increase the shearing strength of fluid, eliminate vortex, improve the circulation of main body, increase the turbulence degree and improve the stirring effect. Meanwhile, the fluctuation of the stirring load can be reduced, and the power consumption is kept stable.
In the above embodiment, in order to observe the fermentation status of the strains or other substances in the biological fermentation tank and to know the fermentation status, the side of the cylinder is connected with an observation window 21 for observing the fermentation status in the cylinder 2.
The present invention has been explained by using specific embodiments, and the explanation of the above embodiments is only used to help understand the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be changes in the specific embodiments and the application range, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (7)
1. The utility model provides a fermentation tank convenient to sample which characterized in that: the device comprises a pipe cap, a cylinder body and an equipment flange, wherein the pipe cap is connected to the lower end of the cylinder body, the equipment flange is connected to the upper end of the cylinder body, an integral jacket is sleeved on the outer side of the cylinder body, a heat-insulating jacket is sleeved on the outer side of the integral jacket, a sampling device is connected to the lower side surface of the cylinder body through a sampling pipeline, and a spraying device is connected to the upper side surface of the cylinder body;
the sampling pipeline penetrates through the integral jacket and the heat-preservation jacket, the sampling pipeline is communicated with the inner cavity of the cylinder, the sampling device comprises a sampling valve, the sampling valve comprises at least 2 communication ports and 1 liquid inlet, the liquid inlet is connected with the sampling pipeline, and one of the communication ports is connected with the steam disinfection pipeline;
spray set includes return bend, connector and sprays the ball, the return bend passes out near the one end of import the lateral wall of barrel, the detachable plug core that is connected with in import of return bend, spray the ball pass through the connector with the return bend is connected, a plurality of sprays the mouth has been seted up to the downside that sprays the ball, the connector has undercut's lower concave part the downside of concave part is offered and is used for the row of the liquid in the return bend and/or the spray ball to the greatest extent mouthful down.
2. The ease of sampling biological fermentor according to claim 1, characterized by: the downside of pipe cap is connected with the landing leg, the quantity of landing leg is 2, 2 the landing leg is followed the axis of pipe cap sets up for the axial symmetry, 2 be provided with tank bottoms valves between the landing leg, tank bottoms valves through discharge pipe with the inner chamber intercommunication of barrel.
3. The ease of sampling biological fermentor according to claim 2, characterized by: the tank bottom valve group comprises at least 2 valve bodies connected with the discharge pipeline, and an included angle of 60-120 degrees is formed between central axes of liquid outlets of the two valve bodies.
4. The ease of sampling biological fermentor according to claim 2, characterized by: the anti-vortex tube comprises a tube cap, and is characterized in that an anti-vortex plate is connected to the bottom of an inner cavity of the tube cap, the bottom of the anti-vortex plate protrudes downwards to form a protruding portion, the protruding portion is connected with the bottom of the tube cap, and a discharge pipeline is located on the lower side of the anti-vortex plate.
5. The ease of sampling biological fermentor according to claim 1, characterized by: the mounting panel is connected to equipment flange upper end, the mounting panel is connected with rabbling mechanism, the rabbling mechanism lower extreme is connected with the (mixing) shaft, the (mixing) shaft stretches into the part of the inner chamber of barrel is connected with the stirring rake.
6. The ease of sampling biological fermentor according to claim 5, characterized by: the downside of stirring rake is connected with stirring baffle, stirring baffle with the inner wall of barrel or the inner wall connection of pipe cap.
7. The ease of sampling biological fermentor according to claim 1, characterized by: the side surface of the cylinder body is connected with an observation window for observing the fermentation condition in the cylinder body.
Priority Applications (1)
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CN202222533622.3U CN217809428U (en) | 2022-09-26 | 2022-09-26 | Biological fermentation tank convenient to sample |
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CN202222533622.3U CN217809428U (en) | 2022-09-26 | 2022-09-26 | Biological fermentation tank convenient to sample |
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CN217809428U true CN217809428U (en) | 2022-11-15 |
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- 2022-09-26 CN CN202222533622.3U patent/CN217809428U/en active Active
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