CN218973959U - One set of immunostaining tool kit for animal tissue and organ - Google Patents

Abstract

The utility model discloses an immunostaining kit for animal tissues and organs, which comprises an I silica gel embedding mould and an II bleaching and dyeing kit or an immunohistochemical wet box. The silica gel embedding mould comprises an upper mould and a lower mould, wherein the inner sides of the upper mould and the lower mould are respectively provided with an upper groove and a lower groove, and the upper groove and the lower groove enclose a cavity structure consistent with the shape of animal tissue organs; the bleaching and dyeing tool box comprises a bleaching and dyeing box body, a bleaching and dyeing filter sleeve plate and a bleaching and dyeing box cover, wherein the upper surface of the bleaching and dyeing box body is downwards extended to form a plurality of independent bleaching and dyeing box body grooves, the bleaching and dyeing filter sleeve plate is provided with a bleaching and dyeing sleeve plate groove which can be accommodated by the bleaching and dyeing box body groove, and the bottom surface of the bleaching and dyeing filter screen is arranged on the bleaching and dyeing filter sleeve plate; the immune grouping wet box comprises a wet box body, a wet box filtering sleeve plate and a wet box cover, wherein the upper surface of the wet box body extends downwards to form a plurality of independent slide grooves, the wet box filtering sleeve plate is provided with a wet box sleeve plate groove which can be accommodated by the slide grooves, and the bottom surface of the wet box filtering sleeve plate is provided with a wet box filter screen.

Description

One set of immunostaining tool kit for animal tissue and organ

Technical Field

The utility model belongs to the technical field of biological experimental equipment, and particularly relates to an immunostaining kit for animal tissues and organs.

Background

Immunohistochemistry is to apply the basic principle of immunology, namely the principle of antigen-antibody reaction, namely the specific combination of antigen and antibody, and to determine the antigen in tissue cells by developing the color developing agent of the labeled antibody through chemical reaction, and to carry out the research of localization, qualitative and relative quantification. Immunostaining methods include bleach-staining and patch-staining, and related experimental tools include tissue embedding molds (for tissue pretreatment), cell culture plates (for bleach-staining), and immunohistochemical wet cartridges (for patch-staining).

Commercial tissue embedding molds are available in different sizes, but are all cube-shaped in shape. When the frozen section embedding medium OCT is used for embedding animal tissue and organs, as the tissue embedding mould is not designed for the shape of the tissue and organs, partial tissue and organs are unevenly coated and the tissue and organ coating angles among different samples are different, when the frozen section embedding medium OCT is used for slicing on an upper machine, the angle is repeatedly adjusted and the embedding medium is trimmed, so that the slices with good effect can be obtained, and the embedding medium is wasted. And the commercial coating box is usually made of transparent plastic material, cannot be stored for a long time in a moisturizing way, and cannot protect tissues with fluorescent signals from light.

The traditional method of bleaching and staining is to use cell culture plates, and the incubation and elution of antibodies are performed by selecting cell culture plates of different specifications according to the number of samples. In the process of incubating and eluting the antibody each time, the tissue slices can be repeatedly transferred, so that time is consumed, irreversible mechanical damage is easily caused to the tissue slices, the later data processing is influenced, and the phenomenon of uneven dyeing easily occurs when the number of dyed samples is large because of the time difference between each two tissue slices. Moreover, if multiple tissue sections are placed in one cell culture plate for staining, the spatial positions of the target tissue region signals in different tissue sections need to be distinguished in a later period, so that the difficulty of statistical analysis of later data is greatly increased, and especially when continuous and highly complete section staining data needs to be acquired, the requirement of using a commercial cell culture plate cannot be met. Therefore, there is a need for an integrated, reusable bleaching and dyeing apparatus that addresses the high throughput, continuity, integrity and specificity of tissue organ sections.

During patch staining, experimenters select immune staining jars or immune grouping wet boxes with different specifications according to the number of samples, when using a commercial staining jar, due to the problem of the angle of placing a glass slide, the situation that a tissue slice falls off from the glass slide after repeated elution can occur, so that the samples are lost, most antibodies are quite expensive, and the wet boxes are selected more. The commercialized immunohistochemical wet box consists of a cover and a base, wherein two water tanks are arranged in the base, and a proper amount of deionized water is required to be added according to experience before the wet box is used to ensure the environmental humidity in the antibody incubation process, so that the antibody evaporation is avoided. When the wet box is used for antibody incubation and elution, a hydrophobic ring is firstly drawn around the patch by means of an immunohistochemical pen, and the elution is dripped into the hydrophobic ring for operation, so that the patch cannot shake randomly, the combination of the antibody and antigens in the tissue of the patch cannot be accelerated by using a shaking table, the phenomenon of uneven dyeing often occurs in patch dyeing, the dyeing effect is quite different compared with bleaching and dyeing, and the patch dyeing mode is only suitable for the dyeing work of thinner (5-30 mu m) sections. Moreover, when the existing immunohistochemical wet box is used, the manual operation of a pipettor is needed to be used repeatedly for each time of elution and incubation of antibodies and reagents, the operation is very careful to avoid the trace of a quick-breaking hydrophobic pen, the hydrophobic pen needs to be rubbed again once being damaged, the experimental operation is tedious and time-consuming, and the hydrophobic pen has volatile and pungent smell and needs to be operated in a fume hood.

Disclosure of Invention

In order to solve the problems, the utility model provides an immunostaining kit for animal tissues and organs, which can protect the original fluorescent signals of tissue samples, save antibodies, protect experimenters, improve the dyeing effect and improve the experimental efficiency.

In order to at least achieve one of the above purposes, the present utility model adopts the following technical scheme:

an immunostaining kit for animal tissue and organs, the immunostaining kit comprising an I silica gel embedding mold and an II bleaching kit or an immunohistochemical wet kit.

The silica gel embedding mould comprises an upper mould and a lower mould, wherein an upper groove is formed in the inner side of the upper mould, a lower groove is formed in the inner side of the lower mould, a cavity structure is formed by surrounding the upper groove and the lower groove, the cavity structure is consistent with the shape of animal tissue organs, and a sample inlet communicated with the cavity structure is formed in the inner side of the mould and can be used for injecting embedding agents.

The utility model provides a dyeing tool box, including the box body that floats, the upside detachable of box body that floats is connected with and floats and dye filter sleeve board and the box cover that floats, the upper surface that floats the box body extends down and forms into a plurality ofly, independent box body recess that floats, it can by to be equipped with on the filter sleeve board that floats to dye the box body recess and hold to float dyes the sleeve board recess, the bottom surface that floats and dye the sleeve board recess is equipped with and floats and dye the filter screen.

The immune grouping wet box comprises a wet box body, wherein a wet box filtering sleeve plate and a wet box cover are detachably connected to the upper side of the wet box body, the upper surface of the wet box body is downwards extended to form a plurality of independent slide grooves for accommodating slides, a wet box sleeve plate groove capable of being accommodated by the slide grooves is formed in the wet box filtering sleeve plate, a wet box filter screen is arranged on the bottom surface of the wet box sleeve plate groove, and a water tank is formed in the periphery of the bleaching and dyeing box body.

Further, the number of grooves of the bleaching box body is 60-360, or the number of slide grooves of the wet box body is 6-36.

Still further, the number of the bleaching cassette recesses is any of the above ranges or values, for example 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, or the number of the slide grooves of the wet cassette is any of the above ranges or values, for example 6, 8, 10, 12, 15, 18, 20, 22, 24, 28, 32, 36, etc., preferably the above bleaching cassette recesses are arranged in an array, for example 6×10, 8×10, 10×12, 12×12, 14×16, 15×15, 18×10, 20×16. Or the slide channels of the wetting cassette are arranged in an array, such as 2 x 3, 2 x 4, 2 x 5, 4 x 3, 5 x 3, 4 x 5, 6 x 6, etc.

Further, the inner surface of the upper mold is provided with mold buckles, the inner side of the lower mold is provided with mold buckle positions corresponding to the mold buckles, and more preferably, the number of the mold buckles is enough to make the mold be sealed, for example, 1-4.

The upper surface of wet box body has seted up wet box and has detained the position, the bottom of wet box lid is equipped with wet box buckle that corresponds with wet box detains the position, wet box detains the position quantity and is enough for making the effect of sealing wet box, for example 2-12. Still further, the number of wet cartridge snap-in positions is 6.

Further, the upper surface of the bleaching and dyeing box cover is provided with a bleaching and dyeing box handle, or the upper surface of the wet box cover is provided with a wet box handle.

Further, the recess of the bleaching cassette is sized to accommodate a tissue slice. Preferably, the recess of the bleaching box is circular, more preferably, the diameter of the circular recess is 10-50mm, which may be any value or any range of the above-mentioned numerical ranges, such as 10, 11, 16, 22, 35, 42, 48, 50mm, etc., or 10-15, 14-25, 10-30, 10-35, 20-50mm, etc.

Further, the cavity structure of the silica gel embedding mold is in a shape consistent with animal tissue and organs, and preferably, the rodent comprises a mouse or a rat. Further preferably, the tissue organ comprises brain tissue, lung, heart, liver, stomach, pancreas, gall bladder, kidney, bladder, uterus, ovary or testis, and the like.

Preferably, the animal comprises a variety of laboratory animals including rodents, rodents; guinea pig family, genus guinea; the genus Lepidoptera, lepidaceae, rabbit; canines; porcine family; a non-human primate; such as mice, rats, guinea pigs, hamsters, dogs; pig, monkey, baboon.

Further, the bleaching and dyeing filter screen is a micron-sized filter screen to prevent animal tissue from entering the bleaching and dyeing box body groove from the bleaching and dyeing box body groove, and simultaneously allow liquid to enter the bleaching and dyeing box body groove from the bleaching and dyeing box body groove.

Similarly, the wet box filter screen is a micron-sized filter screen to prevent tissue from entering the slide groove of the wet box body from the wet box filter sleeve plate groove, and simultaneously, liquid is allowed to enter the wet box filter sleeve plate groove from the slide groove, so that the loss of tissue slices in the experimental process can be reduced.

More preferably, the micron-sized filter has a pore size of 50-200 μm, which may be any of the values or any range of values described above, such as 50, 55, 60, 68, 73, 80, 92, 100, 110, 120, 135, 150, 168, 180, 200 μm, etc., or 50-100, 60-150, 80-120, 90-150 μm, etc.

Further, the wet box filter sleeve plate, when nested on the bleaching and dyeing box body, has a periphery covering the water tank. To prevent volatilization of sterile water in the wet box and maintain the ambient humidity in the box.

Further, the water tank is annular and is arranged around the upper surface of the wet box body.

Further, the bleaching and dyeing box body or the wet box body is provided with a number, and the number can be upper-case or lower-case letters, arabic numerals, characters, figures or other simple marks.

Furthermore, the immunostaining kit further comprises an adaptive wireless vibrator, and the immunohistochemical wet box and the bleaching and dyeing kit can be matched with the adaptive wireless vibrator for use.

Compared with the prior art, the utility model has the following beneficial effects:

1) The silica gel embedding mould can uniformly coat tissue and organs, is easy to operate and acquire high-quality slices, and can protect and save original fluorescence signals of tissue samples to the greatest extent.

2) The bleaching and dyeing tool box is provided with a plurality of bleaching and dyeing box body grooves, the requirement of processing tissue organ continuous slices at one time is met, the bleaching and dyeing filter sleeve plate is ingeniously arranged, the efficiency of experiments is greatly improved, irreversible mechanical damage caused by repeatedly picking the tissue slices is greatly reduced, the consistency of experimental parameters (in elution and incubation processes) between each slice is ensured, the phenomenon of uneven dyeing effect is avoided, the phenomenon that a single groove is matched with one tissue slice is benefited, the spatial position relation of signals in the tissue slices can be distinguished when the dyeing result is finally obtained, and the time of later data analysis is greatly saved.

3) The immune grouping wet box is also provided with the wet box filter sleeve plate and the wet box body, integrates the technical advantages of the dyeing jar and the existing wet box, effectively protects the volatilization of sterile water in the wet box and keeps the environmental humidity in the box, changes the traditional patch dyeing mode, gets rid of the limitation brought by using the hydrophobic pen, can be used by combining with the adaptive wireless vibrator, greatly improves the dyeing effect, is efficient and convenient, and can also prevent the experimenter from being hurt to the body by the volatile reagent of the hydrophobic pen.

4) In the preferred scheme, no matter the tissue slice staining tool box or the wet box for tissue slices, the filter sleeve plates of the tissue slice staining tool box and the wet box for tissue slices adopt micron-sized filter screens, so that the loss of the tissue slices in the experimental process is reduced.

5) In the preferred scheme, the wireless charging design of the wireless vibrator greatly improves the operability of the experiment and the comfort level of the experiment matched with commercial temperature control equipment, and meanwhile, the combination of the wireless vibrator and the wet box for tissue slicing greatly improves the patch dyeing effect.

Drawings

FIG. 1 is a schematic diagram of the inner side structure of a brain tissue silica gel embedding mold according to the present utility model;

FIG. 2 is a schematic view of the inner side structure of the mold for embedding the heart silica gel of the present utility model;

FIG. 3 is a schematic view of the inner side structure of a kidney silica gel embedding mold according to the present utility model;

FIG. 4 is a schematic view of the structure of the bleaching and dyeing tool box of the present utility model;

FIG. 5 is a schematic diagram of the structure of an immunohistochemical wet kit of the present utility model;

the components in the figures are labeled as follows: 1-silica gel embedding mold, 101-upper mold, 102-lower mold, 103-upper groove, 104-lower groove, 105-sample inlet, 106-mold buckle, 107-mold buckle, 2-bleaching and dyeing tool box, 201-bleaching and dyeing box body, 202-bleaching and dyeing box body groove, 203-bleaching and dyeing filter sleeve plate, 204-bleaching and dyeing sleeve plate groove, 205-bleaching and dyeing filter screen, 206-bleaching and dyeing box cover, 207-bleaching and dyeing box handle, 3-immune grouping wet box, 301-wet box body, 302-water tank, 303-slide groove, 304-wet box buckle, 305-wet box filter sleeve plate, 306-wet box sleeve plate groove, 307-wet box filter screen, 308-wet box cover, 309-wet box buckle and 310-wet box handle.

Detailed Description

The advantages and features of the present utility model will become more apparent from the following description when taken in conjunction with the accompanying drawings. It should be understood that the embodiments are illustrative only and should not be taken as limiting the scope of the utility model.

It should be noted that, in the claims and the specification of the present application, the terms "side," "upper," "lower," and the like indicate an orientation or a positional relationship based on the orientation or the positional relationship shown in the drawings, and are not necessarily to be construed as necessarily referring to the apparatus or the component having a specific orientation, being configured or operated in a specific orientation.

In the present utility model, unless explicitly specified and limited otherwise, the terms "fixedly attached," "connected," and the like are to be construed broadly and, for example, as a fixed connection, as a removable connection, or as a unit; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

The utility model comprises a silica gel embedding mould 1 and a bleaching and dyeing tool box 2 or an immunohistochemical wet box 3.

Fig. 1 to 3 show schematic structural views of three tissue and organ silica gel embedding molds in the immunostaining kit of the present utility model.

As in fig. 1, an example is illustrated with brain tissue: the silica gel embedding mould 1 comprises an upper mould 101 and a lower mould 102, wherein an upper groove 103 is formed in the inner side of the upper mould 101, a lower groove 104 is formed in the inner side of the lower mould 102, the upper groove 103 and the lower groove 104 enclose a cavity structure, the cavity structure is consistent with the shape of brain tissue of a mouse, a mould buckle 106 is arranged on the inner surface of the upper mould 101, a mould buckle position 107 corresponding to the mould buckle 106 is formed in the inner side of the lower mould 102, and a sample inlet 105 communicated with the cavity structure is formed in the inner side of the mould 1 so as to facilitate sample injection of reagents such as embedding agents. The sample inlet 105 may be disposed on the upper mold 101, or may be disposed on the lower mold 102, or the sample inlet 105 may be defined when the upper mold 101 and the lower mold 102 are engaged.

In this embodiment, the number of the mold buckles 106 and the corresponding mold buckles 107 is 2, so that the upper mold 101 and the lower mold 102 can be guaranteed to be completely airtight when being attached, and the original fluorescence signal of the brain tissue sample is protected to the greatest extent.

Fig. 2 and 3 show schematic diagrams of cavity structures of a heart tissue organ and a kidney tissue organ of a mouse, respectively.

Of course, the cavity structure may be configured to conform to other tissue organs of the animal, such as the lung, liver, stomach, pancreas, gall bladder, uterus, ovary or testis, etc., and is not intended to be exhaustive. But also other animals than rodents. The shape of animal tissue organs is well known, and reference is made, for example, to "Grant anatomical atlas", etc.

FIG. 4 shows a schematic structural diagram of a bleaching kit in an immunostaining kit according to the present utility model.

The bleaching and dyeing tool box 2 comprises a bleaching and dyeing box body 201, a bleaching and dyeing filter sleeve plate 203 and a bleaching and dyeing box cover 206 are detachably connected to the upper side of the bleaching and dyeing box body 201, the upper surface of the bleaching and dyeing box body 201 extends downwards to form a plurality of independent bleaching and dyeing box body grooves 202, bleaching and dyeing filter sleeve plate grooves 204 which can be accommodated by the bleaching and dyeing box body grooves 202 are formed in the bleaching and dyeing filter sleeve plate 203, the bleaching and dyeing box body grooves 202 are circular, the diameters of the bleaching and dyeing box body grooves 202 are suitable for accommodating tissue slices, bleaching and dyeing filter screens 205 are arranged on the bottom surface of the bleaching and dyeing box body grooves, and the bleaching and dyeing filter screens 205 are micron-level filter screens. The bleaching and dyeing box 201 is provided with Arabic numerals and capital letter numbers. The bleaching and dyeing box 201, the bleaching and dyeing filter sleeve plate 203 and the bleaching and dyeing box cover 206 are all integrally formed, so that the production and the manufacturing are facilitated.

In this embodiment, the number of the grooves 202 of the bleaching and dyeing box body is 120, which satisfies the requirement of disposable tissue and organ continuous slicing, improves the experimental efficiency, reduces the irreversible mechanical damage caused by repeatedly picking slices, and effectively solves the problems of low flux, complicated liquid exchange, uneven antibody incubation and the like of the traditional dyeing tool. The diameter of the bleaching and dyeing filter 205 is 100 μm and the diameter of the circular groove 202 of the circular bleaching and dyeing box is 11mm.

Fig. 5 shows a schematic structural diagram of an immunohistochemical wet kit in the immunostaining kit of the present utility model.

The immunohistochemical wet box 3 comprises a wet box body 301, a wet box filter sleeve 305 and a wet box cover 308 are detachably connected to the upper side of the wet box body 301, the upper surface of the wet box body 301 extends downwards to form a plurality of independent slide grooves 303 for accommodating standard slides, wet box sleeve grooves 306 capable of being accommodated by the slide grooves 303 are formed in the wet box filter sleeve 305, wet box filter screens 307 are arranged on the bottom surfaces of the wet box sleeve grooves 306, water grooves 302 are formed in the peripheries of the bleaching box body 301, and when the wet box filter sleeve 305 is nested on the bleaching box body 301, the peripheries of the wet box filter sleeve grooves 302 are covered by the peripheries of the wet box filter sleeve grooves. The upper surface of wet box lid 308 is equipped with wet box handle 310, and wet box buckling position 304 has been seted up to wet box body 301's upper surface, and wet box lid 308's bottom is equipped with wet box buckle 309 that corresponds with wet box buckling position 304. The wet box body 301, the wet box filter sleeve plate 305 and the wet box cover 308 are all integrally formed, so that the wet box body, the wet box filter sleeve plate and the wet box cover are convenient to produce and manufacture.

In this embodiment, the number of wet box buckles 304 and 309 is 6, and the number of slide slots 303 is 20. The number can also be arranged on the wet box body 301, and the matched design of the slide groove 303 and the wet box sleeve plate groove 306 gets rid of the limitation brought by using the hydrophobic pen, so that the volatilization of sterile water in the wet box and the environmental humidity in the box are effectively protected, the wet box is efficient and convenient, and the experimenter can be prevented from being hurt by the volatile reagent of the hydrophobic pen. The aperture of the wet box screen 307 was 100 μm, reducing tissue slice loss during the experiment.

In order to better understand the technical scheme of the utility model, the following is an example of the use method of the utility model, which is to take the staining work of continuous sections of brain tissue of an adult mouse as an example:

1) Placing the fixed and dehydrated mouse brain tissue into a brain tissue silica gel embedding mould, fastening the two half moulds, inputting OCT from a sample inlet, then transferring to-20 ℃ for freezing, if the experiment is needed, only freezing for 15-20min, and achieving a very uniform coating effect, if the experiment is not needed immediately, storing the mouse brain tissue for a long time at-20 ℃, for example, after 6 months, adhering the coated brain tissue on a freezing head of a frozen slicer by using OCT, adjusting the slicing thickness to 50 mu m for slicing, and obtaining a flat and high-quality slice without repeated trimming.

2) If the bleaching and dyeing tool box is selected for bleaching and dyeing, firstly, a bleaching and dyeing filter sleeve plate is sleeved on the bleaching and dyeing box body, 100 microliters of PBS solution is added into each bleaching and dyeing box body groove, brain slices are sequentially placed into holes of the bleaching and dyeing box body groove according to the slicing sequence, and recording sequence is carried out according to letters and numbers on the bleaching and dyeing box body. After the film collection is finished, covering the bleaching and dyeing box cover, placing the bleaching and dyeing box cover on an adaptive wireless vibrator for OCT (optical coherence tomography) elution, carrying out three times of elution for 10 minutes each time, taking out the bleaching and dyeing filter sleeve plate and placing the bleaching and dyeing box cover above absorbent paper each time, discarding the used PBS in the bleaching and dyeing box body, adding a new PBS solution, re-nesting the bleaching and dyeing filter sleeve plate in the bleaching and dyeing box body, covering the bleaching and dyeing box cover, placing the bleaching and dyeing box cover on the adaptive wireless vibrator for OCT elution, repeating the operation in the mode, and sequentially: blocking (5% BSA in 0.3% PBST solution, 50. Mu.l per well, 1 hour at room temperature) of blocking solution, primary incubation (50. Mu.l per well, for cfos antibody staining, primary dilution concentration 1:1000, 20 hours at 4 ℃), primary elution (PBS wash 3 times, 15 minutes per time, 100. Mu.l per well), secondary incubation (dilution concentration 1:1000, 50. Mu.l per well, 2 hours at room temperature), secondary elution (PBS wash 3 times, 15 minutes per time, 100. Mu.l per well), and final blocking.

3) If the immunohistochemical wet box is selected for patch staining, firstly, the brain slice is sequentially attached to the sticky glass slide according to the slicing sequence, the room temperature is kept for 24 hours, then the wet box filter sleeve plate is sleeved on the wet box body, 800 microliter of PBS solution is added into each hole, the glass slide attached with the brain slice is sequentially placed in the groove of the wet box filter sleeve plate according to the sequence, the wet box cover is covered, the glass slide is placed on an adaptive wireless vibrator for OCT (optical coherence tomography) elution, three times of elution are carried out for 15 minutes each time, each time of liquid exchange only needs to take off the wet box cover, the wet box filter sleeve plate is taken out and placed above absorbent paper, PBS used in a glass slide groove of the wet box body is discarded, the wet box filter sleeve plate is sleeved again after new PBS solution is added, the wet box cover is covered and then placed on the adaptive wireless vibrator for elution, in this way, and the operations of sealing, primary anti-elution, secondary anti-elution and secondary anti-elution are sequentially completed.

The above description is only of the preferred embodiments of the present utility model and is not intended to limit the present utility model, and various modifications and variations may be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims (10)

1. An immune staining kit for animal tissue organs, includes I silica gel embedding mould (1) and II bleaching and dyeing kit (2) or immune group ization wet box (3), its characterized in that:

the silica gel embedding mold (1) comprises an upper mold (101) and a lower mold (102), wherein an upper groove (103) is formed in the inner side of the upper mold (101), a lower groove (104) is formed in the inner side of the lower mold (102), the upper groove (103) and the lower groove (104) enclose a cavity structure, the cavity structure is consistent with the shape of an animal tissue organ, and a sample inlet (105) communicated with the cavity structure is formed in the inner side of the mold (1);

the bleaching and dyeing tool box (2) comprises a bleaching and dyeing box body (201), wherein a bleaching and dyeing filtering sleeve plate (203) and a bleaching and dyeing box cover (206) are detachably connected to the upper side of the bleaching and dyeing box body (201), the upper surface of the bleaching and dyeing box body (201) is extended downwards to form a plurality of independent bleaching and dyeing box body grooves (202), bleaching and dyeing filtering sleeve plate grooves (204) which can be contained by the bleaching and dyeing box body grooves (202) are formed in the bleaching and dyeing filtering sleeve plate (203), and bleaching and dyeing filter screens (205) are arranged on the bottom surface of the bleaching and dyeing sleeve plate grooves (204);

the immune grouping wet box (3) comprises a wet box body (301), a wet box filter sleeve plate (305) and a wet box cover (308) are detachably connected to the upper side of the wet box body (301), the upper surface of the wet box body (301) is downwards extended to form a plurality of slide grooves (303) which are independent and contain slides, a wet box sleeve plate groove (306) which can be contained by the slide grooves (303) is arranged on the wet box filter sleeve plate (305), a wet box filter screen (307) is arranged on the bottom surface of the wet box sleeve plate groove (306), and a water groove (302) is formed in the periphery of the wet box body (301).

2. The immunostaining kit of claim 1, wherein: the number of the bleaching box grooves (202) is 60-360, or the number of the slide grooves (303) of the wet box is 6-36.

3. Immunostaining kit according to claim 1 or 2, characterized in that: the inner surface of the upper die (101) is provided with a die buckle (106), the inner side of the lower die (102) is provided with a die buckle position (107) corresponding to the die buckle (106), or the upper surface of the wet box body (301) is provided with wet box buckle positions (304), the bottom of the wet box cover (308) is provided with wet box buckles (309) corresponding to the wet box buckle positions (304), and the number of the wet box buckle positions (304) is 2-12.

4. Immunostaining kit according to claim 1 or 2, characterized in that: the upper surface of the bleaching and dyeing box cover (206) is provided with a bleaching and dyeing box handle (207), or the upper surface of the wet box cover (308) is provided with a wet box handle (310).

5. Immunostaining kit according to claim 1 or 2, characterized in that: the bleaching cassette recess (202) is sized to receive a tissue slice.

6. The immunostaining kit of claim 1, wherein: the shape of the cavity structure of the silica gel embedding mould (1) is consistent with the tissue and organ of animals, and the animals are from experimental animals, including experimental animals from murine, guinea pig, true rabbit, canine, porcine or non-human primate.

7. The immunostaining kit of claim 1 or 6, wherein: the tissue organ comprises brain tissue, lung, heart, liver, stomach, pancreas, gall bladder, kidney, bladder, uterus, ovary or testis.

8. Immunostaining kit according to claim 1 or 2, characterized in that: when the wet box filter sleeve plate (305) is nested on the wet box body (301), the periphery of the wet box filter sleeve plate (305) covers the water tank (302).

9. Immunostaining kit according to claim 1 or 2, characterized in that: the bleaching filter screen (205) and the wet box filter screen (307) are micron-sized filter screens, and the pore diameter of the filter screens is 50-200 mu m.

10. Immunostaining kit according to claim 1 or 2, characterized in that: the immunostaining kit further comprises an adaptive wireless vibrator.

Images