CN216309595U

CN216309595U - Fast immunohistochemical film developing device

Info

Publication number: CN216309595U
Application number: CN202121917587.4U
Authority: CN
Inventors: 王新娟; 郭静竹; 韩娜; 郁卫东
Original assignee: Peking University Peoples Hospital
Current assignee: Peking University Peoples Hospital
Priority date: 2021-08-13
Filing date: 2021-08-13
Publication date: 2022-04-15
Anticipated expiration: 2031-08-13

Abstract

The utility model relates to a quick immunohistochemical film washer, which comprises a box body, wherein a plurality of washing grooves are arranged on the box body, and a fishing net is arranged in each washing groove; the scoop net is freely removable from the wash tank. The utility model solves the problems of time consumption for cleaning and easy damage of the tissue slices in the immunohistochemical process of the brain tissue slices, and improves the efficiency of the experiment and the quality of the experiment result.

Description

Fast immunohistochemical film developing device

Technical Field

The utility model relates to the technical field of film washing equipment for biomedical immunohistochemical experiments, in particular to a quick immunohistochemical film washer.

Background

Immunohistochemistry is an important technique in life science and medical research. It is a one-door staining technique established by carrying out antigen-antibody reaction from the level of tissue cells and displaying dynamic change of biological macromolecules on tissue slices in situ. The method comprises the following basic steps: obtaining tissue sections, repairing antigens, breaking membranes, sealing, incubating primary antibodies and secondary antibodies, developing, imaging and the like. Essentially each step is followed by washing the tissue section with phosphate buffered saline. In the study of central nervous brain, immunohistochemistry of mouse brain tissue section is basically performed by using a floating slice method. The floating method is different from the patch method, and the brain tissue section is not attached to a glass slide for operation. Thus, the washing of the brain slice at each step becomes troublesome.

At present, the mainly adopted slice fishing device is mainly a writing brush and the like, brain tissues need to be transferred one by one, so that the time cost is increased, tissue slices are easily damaged, and the experimental result is influenced.

SUMMERY OF THE UTILITY MODEL

Aiming at the problems, the utility model aims to provide a quick immunohistochemical film washer, which can solve the problems of time consumption for cleaning and easy damage of tissue slices in the immunohistochemical process of brain tissue slices so as to improve the efficiency and quality of experiments.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the quick immunohistochemical film washer comprises a box body, wherein a plurality of cleaning grooves are arranged on the box body, and a fishing net is arranged in each cleaning groove; the scoop net is freely removable from the wash tank.

The fast immunohistochemical film developing device is characterized in that the scoop net comprises a scoop net body, a handle and a screen, the scoop net body is of a cylindrical structure with an upper opening and a lower opening, the handle is arranged at the edge of the upper opening of the scoop net body, and the screen is arranged at the opening of the lower part of the scoop net body and is tightly connected with the edge of the lower opening of the scoop net body.

The quick immunohistochemical film developing device is characterized in that the cleaning tank is preferably of a cylindrical structure matched with the shape of the fishing net body.

The quick immunohistochemical film washer is characterized in that preferably, the screen mesh is a nylon mesh.

The quick immunohistochemical film developing device is characterized in that the cleaning tanks are preferably arranged on the box body in an array mode.

The quick immunohistochemical film washer preferably further comprises a box cover, wherein the box cover covers the box body, and the box cover is connected with the box body through the matching of a buckle of the box cover and a buckle of the box body.

Due to the adoption of the technical scheme, the utility model has the following advantages:

the utility model solves the problems of time consumption for cleaning and easy damage of the tissue slices in the immunohistochemical process of the brain tissue slices, and improves the efficiency of the experiment and the quality of the experiment result.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is an enlarged schematic view of a portion a in fig. 1.

The figures are numbered:

1-box body; 2-a box cover; 3-buckling a box cover; 4, buckling a box body; 5, cleaning a tank; 6-fishing net; 601-a scoop net body; 602-a grip; 603-screen mesh.

Detailed Description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the utility model can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended to limit the scope of the present invention, but are merely intended to illustrate the spirit of the technical solution of the present invention.

The utility model provides a quick immunohistochemical film washer, which comprises a box body, wherein the box body is provided with a plurality of cleaning tanks, and each cleaning tank is internally provided with a fishing net; the scoop net is freely removable from the wash tank. The utility model solves the problems of time consumption for cleaning and easy damage of the tissue slices in the immunohistochemical process of the brain tissue slices, and improves the efficiency of the experiment and the quality of the experiment result.

As shown in fig. 1, the fast immunohistochemical film washer provided by the utility model comprises a box body 1, wherein a plurality of washing tanks 5 are arranged on the box body 1, and a fishing net 6 is arranged in each washing tank 5; the scoop net 6 can be freely removed from the wash bowl 5.

In the above embodiment, preferably, as shown in fig. 2, the scoop 6 includes a scoop body 601, a handle 602 and a screen 603, the scoop body 601 is a cylindrical structure with an upper opening and a lower opening, the handle 602 is disposed at the edge of the upper opening of the scoop body 601, and the screen 603 is disposed at the lower opening of the scoop body 601 and is tightly connected with the edge of the lower opening of the scoop body 601.

In the above embodiment, preferably, the cleaning tank 5 is a cylindrical structure adapted to the shape of the scoop body 601.

In the above embodiment, preferably, the mesh 603 is a nylon mesh.

In the above embodiment, it is preferable that the washing tanks 5 are arranged in an array on the cartridge body 1.

In the above embodiment, preferably, the present invention further comprises a box cover 2, wherein the box cover 2 covers the box body 1 and is connected by the matching of the box cover buckle 3 and the box body buckle 4. Therefore, the box body 1 can be sealed, the cleaning liquid can be prevented from being poured, the tissue section is cleaned in a relatively closed environment, and the influence of surrounding adverse factors is reduced.

When tissue slices are washed, after phosphate buffer saline solution is added into a washing tank 5, a fishing net 6 is put into the washing tank, and then the tissue slices are put into the washing tank; the whole box body 1 is placed on a shaking table, and after the tissue slices are washed once, the fishing net 6 and the tissue slices are moved into a next washing tank 5 containing phosphate buffer salt solution together, so that the washing liquid is updated, and a new round of washing is performed. Therefore, the tedious process of repeatedly fishing each tissue slice and the damage to the tissue are avoided, the time and the cost are saved, the experiment quality is ensured, and the rapid and nondestructive cleaning of the tissue can be realized.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A quick immunohistochemical film washer is characterized by comprising a box body, wherein a plurality of cleaning tanks are arranged on the box body, and a fishing net is arranged in each cleaning tank; the fishing net can be freely taken out of the cleaning tank;

the scoop net comprises a scoop net body, a handle and a screen, the scoop net body is a cylindrical structure with an upper opening and a lower opening, the handle is arranged at the edge of the upper opening of the scoop net body, and the screen is arranged at the lower opening of the scoop net body and is closely connected with the edge of the lower opening of the scoop net body.

2. The rapid immunohistochemical processor of claim 1, wherein the cleaning tank is a cylindrical structure adapted to the shape of the scoop body.

3. The instant immunohistochemical processor of claim 1, wherein the screen is a nylon mesh.

4. The instant immunohistochemical processor of claim 1, wherein the washing wells are arrayed on the cartridge.

5. The rapid immunohistochemical film washer according to any one of claims 1 to 4, further comprising a box cover, wherein the box cover covers the box body and is connected through the matching of the box cover buckle and the box body buckle.