CN110658047B - Animal experiment pathology specimen fixing, dehydrating and decalcification system and method - Google Patents
Animal experiment pathology specimen fixing, dehydrating and decalcification system and method Download PDFInfo
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- CN110658047B CN110658047B CN201911101577.0A CN201911101577A CN110658047B CN 110658047 B CN110658047 B CN 110658047B CN 201911101577 A CN201911101577 A CN 201911101577A CN 110658047 B CN110658047 B CN 110658047B
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- 238000002474 experimental method Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims description 34
- 230000007170 pathology Effects 0.000 title claims description 12
- 239000007788 liquid Substances 0.000 claims abstract description 236
- 238000002791 soaking Methods 0.000 claims abstract description 81
- 238000002347 injection Methods 0.000 claims abstract description 41
- 239000007924 injection Substances 0.000 claims abstract description 41
- 230000001575 pathological effect Effects 0.000 claims abstract description 14
- 238000007789 sealing Methods 0.000 claims description 21
- 238000007599 discharging Methods 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- 210000001503 joint Anatomy 0.000 claims description 7
- 230000018044 dehydration Effects 0.000 claims description 3
- 238000006297 dehydration reaction Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 230000005484 gravity Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 2
- 210000001557 animal structure Anatomy 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 229920002866 paraformaldehyde Polymers 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 210000000609 ganglia Anatomy 0.000 description 1
- 239000000819 hypertonic solution Substances 0.000 description 1
- 229940021223 hypertonic solution Drugs 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The embodiment of the invention provides a system for fixing, dehydrating and decalcification of pathological specimens in animal experiments, which comprises a liquid storage tank, a liquid discharge plate, a plurality of liquid discharge funnels and a liquid discharge control mechanism, wherein the liquid discharge component comprises a liquid discharge groove, a liquid discharge plate, a plurality of liquid discharge funnels and a liquid discharge control mechanism, the liquid discharge control mechanism comprises a plurality of valves, a valve rod, a connecting rod and a handle, the valves are respectively arranged at the bottoms of the liquid discharge funnels, when the soaking liquid needs to be replaced, a liquid injection plate of the liquid injection component is arranged on the fixed plate, new soaking liquid flows into the liquid injection funnels from the liquid injection plate and then flows into a specimen tube, so that the soaking liquid in a plurality of specimen tubes can be replaced once, the replacing speed is high, the duration of the time difference is short after the old soaking liquid is discharged, the time of exposing the specimen to the air is shortened, and the specimen is not required to be removed from the specimen tube, so that pollution caused by contact with an external bearing carrier is avoided.
Description
Technical Field
The invention relates to the technical field of biomedicine, in particular to a system and a method for fixing, dehydrating and decalcification of an animal experiment pathology specimen.
Background
In the later stage of the biomedical animal experiment, the obtained animal organ pathology specimen needs to be fixed in a container by a fixing solution or dehydrated in a hypertonic solution, and if the animal organ pathology specimen relates to a skeletal system, decalcification treatment is also needed for later pathological detection. In this process, the fixing liquid, the hypertonic liquid and the decalcification liquid need to be replaced for a plurality of times. At present, an EP tube is used for accommodating a specimen in a laboratory, the specimen is required to be taken out after the liquid is replaced, the invalid liquid is poured out, and the specimen is placed into new liquid for treatment. The original structure of directly using the sample tube to soak the sample tissue has a plurality of defects, for example, the replacement needs one by one when replacing, the replacement speed is low, the sample tissue needs to be taken out and placed on an external carrier when replacing, the pollution of the carrier to the sample exists, and the risk of the pollution of the external environment to the sample also exists.
Disclosure of Invention
It is necessary to provide a system for fixing, dehydrating and decalcification of pathological specimens in animal experiments.
It is also necessary to provide a method for fixing, dehydrating and decalcification of pathological specimens of animal experiments.
The utility model provides an animal experiment pathology sample is fixed, dehydration, decalcification system, includes flowing back subassembly, fixed subassembly, soaking subassembly, annotates the liquid subassembly, and the flowing back subassembly includes reservoir, flowing back board, a plurality of flowing back funnels, flowing back control mechanism, reservoir internally mounted holds the cavity, and the flowing back board sets up in the reservoir top, sets up a plurality of mounting holes on the flowing back board, and a plurality of flowing back funnels are installed on the mounting hole, and the upper end of flowing back funnels is fixed in on the flowing back board, and the lower extreme is used for discharging soaking liquid, and flowing back control mechanism sets up in the below of flowing back funnels, and flowing back control mechanism includes a plurality of valves, valve rod, connecting rod, handle, and a plurality of valves set up respectively in the bottom of flowing back funnels, and the valve is connected to the one end of valve to the control valve, and the other end of connecting rod passes the lateral wall of reservoir, sets up the handle at the tip of connecting rod, the fixed subassembly includes stand column, fixed plate setting up in flowing back board top, stand column setting up between fixed plate and flowing back board for installing the soaking subassembly on the mounting hole, soaking subassembly including a plurality of tubes, soaking liquid, the bottom of tubes is set up in the bottom of the funnels, the bottom of the filling plate, the sample is placed in the bottom of the filling plate, is filled in the bottom of the sample, is filled in the bottom of the filling, is filled in the sample.
Preferably, a flexible filter screen is further arranged inside the specimen tube so as to prevent the specimen from being discharged along with the liquid during liquid discharge.
Preferably, the specimen tube comprises a soaking tube, a connecting tube and a sealing cover, wherein the soaking tube is a common test tube, the lower end of the soaking tube is in an inclined funnel shape, the connecting tube is arranged at the bottom of the soaking tube, an external thread is arranged on the outer wall of the connecting tube, and an internal thread is further arranged on the inside of the neck of the liquid draining funnel so as to be in butt joint with the external thread of the connecting tube.
Preferably, the bottom of the liquid injection funnel is higher than the mouth part of the specimen tube, so that the bottom of the liquid injection funnel is prevented from extending into the specimen tube to pollute the soaking liquid in the specimen tube.
Preferably, the mouth of the liquid filling funnel is flush with the mounting hole of the bottom of the liquid containing disc, so that the soaking liquid in the liquid containing disc flows into the liquid filling funnel, and the outer wall of the liquid filling funnel is in sealing connection with the mounting hole.
Preferably, the end of the valve rod is hinged with the connecting rod so as to enable the valve rod to rotate relative to the connecting rod.
Preferably, a drain pipe is further provided on the side wall of the drain tank, and a main valve is provided on the drain pipe.
A method for fixing, dehydrating and decalcifying an animal experimental pathological specimen by using the animal experimental pathological specimen fixing, dehydrating and decalcifying system, which comprises the following steps:
s1, placing a specimen in a specimen tube filled with a soaking solution, and keeping the test for required time;
s2, when the soaking liquid needs to be replaced, removing a sealing cover of the opening of each sample tube, installing a liquid injection assembly above the fixed assembly, and keeping a liquid injection funnel of the liquid injection assembly above the sample tubes;
s3, opening a valve of a liquid discharge control mechanism, and discharging all used invalid soaking liquid in the sample tubes at one time;
s4, closing a valve of the liquid discharge control mechanism;
s5, pouring new soaking liquid into the liquid injection tray, and filling each sample tube with the new soaking liquid;
and S6, removing the liquid injection assembly, covering the mouth of each specimen tube with a sealing cover, and keeping the experiment for required time.
A method for fixing, dehydrating and decalcifying an animal experiment pathology specimen comprises the following steps:
s1, placing a specimen in a specimen tube filled with a soaking solution, and keeping the test for required time;
s2, when the soaking liquid needs to be replaced, removing a sealing cover of each sample tube opening, installing a liquid injection assembly above the fixed assembly, and keeping a liquid injection funnel of the liquid injection assembly above the sample tubes;
s3, opening a valve of a liquid discharge control mechanism, pouring new soaking liquid into the liquid injection tray, discharging all used soaking liquid in the sample tubes at one time, and filling the sample tubes with the new soaking liquid;
s4, closing a valve of the liquid discharge control mechanism;
and S5, removing the liquid injection assembly, covering the mouth of each specimen tube with a sealing cover, and keeping the experiment for required time.
In the invention, the liquid needs to be changed for many times in the pathological specimen treatment process, for example, the whole decalcification process needs to be completed for one month or even several months, the soaking liquid is changed once in several days in the decalcification period, and under the condition of large specimen quantity, the traditional method is to pour out the liquid one by one and then change the liquid one by one, so that the process is very time-consuming. The device only needs to discharge the used old liquid in all the sample tubes in batch by opening the liquid discharge control mechanism once, and then injects the new soaking liquid in batch by the liquid injection assembly, thereby realizing liquid exchange and greatly saving liquid exchange time.
In the traditional method, the tissue is taken out from the liquid in the liquid exchange process, and in the process, the sample is placed in the external environment and is easily polluted by the external environment, or enzymes or other factors to be detected in the tissue are damaged due to the difference of temperature or pH value. In the liquid exchange process, the device does not need to take out the sample, and avoids the contact between the sample and the external environment, thereby avoiding the problems.
In the traditional method, the tissue is required to be taken out from the liquid in the liquid exchange process, and the tissue is usually required to be clamped by using ophthalmic forceps or microscopic forceps, and in the process, softer specimens such as brain, spinal cord and the like are extremely easy to damage due to repeated clamping. And for some smaller specimens, such as nerves and ganglia, the specimens are easily lost in the process of replacing the fluid for many times, so that the specimens are lost. The device is provided with the flexible filter screen at the lower end interface, so that a specimen does not need to be clamped for multiple times in the liquid discharge process, the damage of the specimen is avoided, and the loss of the specimen is prevented.
The traditional method needs to remove the specimen tube filled with the specimen to the side of the water tank, and the bottom of the device is provided with a liquid storage tank and a connecting pipe, so that the waste liquid can be directly drained to the waste liquid tank or the water tank for discharging.
The traditional method is exposed in the liquid exchange process, such as formalin or paraformaldehyde with most of fixed liquid which is inflammable, explosive, volatile, toxic and carcinogenic, and the laboratory environment can be polluted by the long-time exposure in the liquid exchange process, so that potential safety hazards are caused. The device effectively reduces the exposure time, is relatively airtight in the liquid discharge process, and effectively reduces the volatilization amount of the fixed liquid, thereby avoiding the problems.
After the traditional method uses the specimen tube, most of the specimen tubes are directly discarded, so that the resource waste is caused and the environment is easy to pollute. If the sample tube needs to be cleaned again, the cleaning process needs to be performed by cleaning liquid brushing for more than 15 times, tap water flushing for more than 3 times, deionized water flushing is performed, and the sample tube can only be singly flushed and cannot be operated in batches, so that the process efficiency is low and the time is consumed. After decalcification of this device finishes, take out the sample, put into this device again with the sample pipe, place annotate the liquid subassembly in the backup pad, empty washing liquid, running water, deionized water in proper order and annotate the liquid inslot, washing liquid automatic downward flow sample pipe, flowing back funnel, to its self-cleaning, keep the washing time, reach many times abluent purpose, can carry out batch operation like this, and the flowing water washing effect is more lavage effect better.
Drawings
Fig. 1 is a schematic structural diagram of a system for fixing, dehydrating and decalcification of an animal experimental pathological specimen.
Fig. 2 is a partial cross-sectional view of fig. 1.
Fig. 3 is a partial enlarged view of fig. 2.
Fig. 4 is an exploded view of fig. 1.
Fig. 5 is a cross-sectional view of the tray.
Fig. 6 and 7 are schematic diagrams of the structure of the sample tube.
Fig. 8 is a cross-sectional view of the present device.
Fig. 9 is a partial enlarged view of fig. 8.
In the figure: reservoir 11, discharge tubule 111, discharge plate 12, discharge funnel 13, discharge tube 131, valve 14, valve stem 15, link 16, handle 17, column 21, fixing plate 22, sample tube 31, immersing tube 311, connecting tube 312, cover 313, flexible filter 314, liquid containing tray 41, and liquid filling funnel 42.
Detailed Description
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Referring to fig. 1-7, the embodiment of the invention provides a system for fixing, dehydrating and decalcification of pathological specimens in animal experiments, which comprises a liquid discharge assembly, a fixing assembly, a soaking assembly and a liquid injection assembly, wherein the liquid discharge assembly comprises a liquid storage tank 11, a liquid discharge plate 12, a plurality of liquid discharge funnels 13 and a liquid discharge control mechanism, a containing cavity is arranged in the liquid storage tank 11, the liquid discharge plate 12 is arranged above the liquid storage tank 11, a plurality of mounting holes are formed in the liquid discharge plate 12, a plurality of liquid discharge funnels 13 are arranged on the mounting holes, the upper end of the liquid discharge funnels 13 is fixed on the liquid discharge plate 12, the lower end of the liquid discharge funnels 13 is used for discharging soaking liquid, the liquid discharge control mechanism is arranged below the liquid discharge funnels 13, the liquid discharge control mechanism comprises a plurality of valves 14, a valve rod 15, a connecting rod 16 and a handle 17, the valves 14 are respectively arranged at the bottom of the liquid discharge funnels 13, one end of the valve rod 15 is connected with the valve 14, the other end of the connecting rod 16 penetrates through the side wall of the liquid storage tank 11, a handle 17 is arranged at the end part of the connecting rod 16, the fixing component comprises a stand column 21 and a fixing plate 22, the fixing plate 22 is arranged above the liquid discharge plate 12, the stand column 21 is arranged between the fixing plate 22 and the liquid discharge plate 12, a plurality of mounting holes are formed in the fixing plate 22 and used for mounting a soaking component, the soaking component comprises a plurality of sample tubes 31, a soaking liquid is contained in the sample tubes 31, the sample is soaked in the soaking liquid of the sample tubes 31, the upper ends of the sample tubes 31 are inserted into the mounting holes of the mounting plate, the lower ends of the sample tubes are in butt joint with the liquid discharge hopper 13, the liquid injection component comprises a liquid containing disc 41 and a plurality of liquid injection hoppers 42, the bottom of the liquid containing disc 41 is provided with supporting legs and used for being placed above the fixing plate 22, a plurality of mounting holes are formed in the disc bottom of the liquid containing disc 41, the filling funnel 42 is disposed in the mounting hole, and a lower end of the filling funnel 42 is disposed at a mouth of the specimen tube 31.
Further, a flexible filter screen 314 is further provided inside the specimen tube 31 to prevent the specimen from being discharged with the liquid during the liquid discharge, and to prevent the specimen from being damaged by the liquid discharge process or the extrusion of the specimen in a narrow space at the bottom after the liquid discharge.
Further, the specimen tube 31 includes a soaking tube 311, a connecting tube 312, and a sealing cap 313, the soaking tube 311 is a common test tube, the lower end of the soaking tube 311 is in a slant funnel shape, the connecting tube 312 is mounted at the bottom of the soaking tube 311, an external thread is provided on the outer wall of the connecting tube 312, a liquid discharge tube 131 is further provided at the bottom of the liquid discharge funnel 13, the liquid discharge tube 131 extends downward to a length greater than that of the connecting tube, an internal thread is further provided on the inside of the neck of the liquid discharge tube 131 to be in butt joint with the external thread of the connecting tube 312, a valve 14 is provided on the outer wall of the liquid discharge tube 131, and the sealing cap 313 is detachably connected with the soaking tube 311 in a sealing manner. Since the dehydration and decalcification process requires 1 month or several months, the connection tube 312 of the sample tube 31 is screwed to the drain tube in this process, so that the connection is stable and the connection can be detachably performed. The sample tube adopts the spiral interface can also conveniently take a certain sample alone and conveniently take off freely when doing experimental study to the inclined plane bottom and the liquid discharge funnel 13 inclined plane butt joint of connecting pipe 312 not only stably connect, and the inclined plane also helps the liquid to discharge moreover, is difficult for deposit impurity or sediment.
For example, the mouth of the cover 313 and the mouth of the immersing tube 311 are screwed together, and a seal ring is provided on the inner wall of the cover 313, and after tightening, the sealing connection is achieved. Thus, when realizing in this patent whole flowing back in batches, pour into new liquid into, still can be to a sample pipe individual operation, for example, the decalcification of a certain sample pipe is accomplished, when needing to take out this individual sample pipe, can screw up the valve 14 of this sample pipe below, screw up in the bar closing cap, then screw up soaking tube 311, make its bottom and the separation of flowing back funnel 13, then lift this sample pipe, take out, because closing cap sealing operation this moment, connecting pipe 312 bottom opening is also less for the liquid in this sample pipe keeps, can not leak from the bottom, so can take out this sample pipe alone, realized the purpose of individual operation, two purposes are achieved. See fig. 8, 9. Labels which can be scrubbed and reused for many times are arranged on the top of the sealing cover 313 and the side wall of the soaking tube 311, so that the specimen information can be marked conveniently.
Further, the bottom of the filling funnel 42 is higher than the mouth of the sample tube 31, so that the bottom of the filling funnel 42 is prevented from extending into the sample tube 31 to pollute the immersion liquid in the sample tube 31.
Further, the mouth of the filling funnel 42 is flush with the mounting hole of the bottom of the liquid containing plate 41, so that the soaking liquid in the liquid containing plate 41 flows into the filling funnel 42, and the outer wall of the filling funnel 42 is in sealing connection with the mounting hole. When changing the soak, place the annotate liquid dish of annotating the liquid subassembly on fixed plate 22, again with new soak introduction annotate the liquid in the liquid dish, flow into annotate liquid funnel 42 by annotating the liquid dish, and then flow into in the sample pipe 31, realized once only changing the soak in a plurality of sample pipes 31 like this, and change fastly, also make old soak discharge back before new soak gets into, this time difference duration is shorter, thereby make the time that the sample was exposed in the air shorten, and this mode, need not remove sample pipe 31 with the sample, avoided the pollution that contacts with outside carrier and cause.
Further, the end of the valve rod 15 is hinged to the connecting rod 16, so that the valve rod 15 rotates relative to the connecting rod 16. When liquid discharge is needed, only the handle is needed to be pulled, the connecting rod 16 moves to one side, and the valve rod 15 is driven to rotate by 90 degrees, so that the valve 14 is opened, and liquid discharge is realized.
Further, a drain pipe 111 is provided on the drain tank side wall, and a main valve is provided on the drain pipe 111.
The invention also provides a method for fixing, dehydrating and decalcification of the animal experimental pathological specimen by using the animal experimental pathological specimen fixing, dehydrating and decalcification system, which comprises the following steps:
s1, placing a specimen in a specimen tube 31 filled with a soaking solution, and keeping the test for required time;
s2, when the soaking liquid needs to be replaced every three days, removing a sealing cover 313 at the opening part of each sample tube 31, installing a liquid injection assembly above the fixed assembly, and keeping a liquid injection funnel 42 of the liquid injection assembly above the sample tubes 31;
s3, opening the valve 14 of the liquid discharge control mechanism, and discharging all used soaking liquid in the sample tube 31 at one time;
s4, closing a valve 14 of the liquid discharge control mechanism;
s5, pouring new soaking liquid into the liquid injection tray, and filling each sample tube 31 with the new soaking liquid;
s6, removing the liquid injection assembly, covering the opening of each sample tube 31 with a sealing cover 313, and keeping the experiment for required time.
The invention also provides a method for fixing, dehydrating and decalcification of the pathological specimen of the animal experiment, which comprises the following steps:
s1, placing a specimen in a specimen tube 31 filled with a soaking solution, and keeping the test for required time;
s2, when the soaking liquid needs to be replaced every three days, removing a sealing cover 313 at the opening part of each sample tube 31, installing a liquid injection assembly above the fixed assembly, and keeping a liquid injection funnel 42 of the liquid injection assembly above the sample tubes 31;
s3, opening a valve 14 of the liquid discharge control mechanism, simultaneously pouring new soaking liquid into the liquid injection tray, discharging all used soaking liquid in the sample tubes 31 at one time, and simultaneously filling the sample tubes 31 with the new soaking liquid;
s4, closing a valve 14 of the liquid discharge control mechanism;
s5, removing the liquid injection assembly, covering the opening of each sample tube 31 with a sealing cover 313, and keeping the experiment for required time.
In this scheme, go on with the soaking solution that the bottom was discharged just and pour into new soaking solution in step, avoided when separately operating, just obtain the whole discharge of soaking solution, when new soaking solution had not been pour into yet, the sample exposes in the air, by the risk of impurity pollution in the air. In this scheme, new soak and old soak seamless butt joint for the sample is in the soak all the time, does not have the problem of contacting with the air.
Because the specific gravity of the soaking liquid is smaller than the density of the specimen, the specimen is sunk at the bottom of the specimen tube 31 at any time, and the old soaking liquid has a problem of slight volatilization after three days of use, so that the specific gravity of the old soaking liquid is slightly larger than that of the new soaking liquid, and the old soaking liquid is actively discharged downwards due to the slightly heavier specific gravity when the new soaking liquid is added while the old soaking liquid is discharged, and the problem of unclean discharge of the old soaking liquid does not exist.
For example, in the present invention, formalin or paraformaldehyde is selected as the soaking solution.
The modules or units in the device of the embodiment of the invention can be combined, divided and deleted according to actual needs.
The foregoing description of the preferred embodiment of the invention will, of course, not be construed as limiting the scope of the invention, but rather as being embodied in other specific forms without departing from the spirit or essential characteristics thereof. Those skilled in the art will recognize that the invention can be practiced with modification within the spirit and scope of the appended claims. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is intended to include only a single embodiment, and that this description is for clarity only, and that the disclosure is not limited to only a single embodiment, as other embodiments may be suitably combined in various embodiments as will be apparent to those of skill in the art.
Claims (6)
1. An animal experiment pathology sample fixing, dehydration and decalcification system is characterized in that: comprises a liquid discharge assembly, a fixing assembly, a soaking assembly and a liquid injection assembly, wherein the liquid discharge assembly comprises a liquid storage tank, a liquid discharge plate, a plurality of liquid discharge funnels and a liquid discharge control mechanism, a containing cavity is arranged in the liquid storage tank, the liquid discharge plate is arranged above the liquid storage tank, a plurality of mounting holes are formed in the liquid discharge plate, the plurality of liquid discharge funnels are arranged on the mounting holes, the upper ends of the liquid discharge funnels are fixed on the liquid discharge plate, the lower ends of the liquid discharge funnels are used for discharging soaking liquid, the liquid discharge control mechanism is arranged below the liquid discharge funnels, the liquid discharge control mechanism comprises a plurality of valves, a valve rod, a connecting rod and a handle, the valves are respectively arranged at the bottoms of the liquid discharge funnels, one ends of the valve rod are connected with the valves to control the opening and closing of the valves, the other ends of the valve rod are connected with one ends of the connecting rod, the other ends of the connecting rod penetrate through the side wall of the liquid storage tank, the end part of the connecting rod is provided with a handle, the fixing component comprises a stand column and a fixing plate, the fixing plate is arranged above the liquid discharging plate, the stand column is arranged between the fixing plate and the liquid discharging plate, a plurality of mounting holes are formed in the fixing plate and used for mounting the soaking component, the soaking component comprises a plurality of sample tubes, soaking liquid is contained in the sample tubes, the samples are soaked in the soaking liquid of the sample tubes, the upper ends of the sample tubes are inserted into the mounting holes of the mounting plate, the lower ends of the sample tubes are in butt joint with the liquid discharging funnel, the liquid filling component comprises a liquid containing disc and a plurality of liquid filling funnels, the bottom of the liquid containing disc is provided with supporting legs and used for being placed above the fixing plate, a plurality of mounting holes are formed in the disc bottom of the liquid containing disc, the liquid filling funnels are arranged in the mounting holes, and the lower ends of the liquid filling funnels are placed at the mouth parts of the sample tubes; a flexible filter screen is arranged in the specimen tube to avoid the discharge of the specimen along with the liquid during the liquid discharge; the specimen tube comprises a soaking tube, a connecting tube and a sealing cover, wherein the soaking tube is a common test tube, the lower end of the soaking tube is in an inclined funnel shape, the connecting tube is arranged at the bottom of the soaking tube, external threads are arranged on the outer wall of the connecting tube, and internal threads are further arranged on the inside of the neck of the liquid draining funnel so as to be in butt joint with the external threads of the connecting tube.
2. The animal experiment pathology specimen fixing, dehydrating and decalcification system according to claim 1, wherein: the height of the bottom of the liquid injection funnel is higher than that of the opening of the sample tube, so that the bottom of the liquid injection funnel is prevented from extending into the sample tube to pollute the soaking liquid in the sample tube.
3. The animal experiment pathology specimen fixing, dehydrating and decalcification system according to claim 1, wherein: the mouth of the liquid injection funnel is flush with the mounting hole of the bottom of the liquid containing disc, so that the soaking liquid in the liquid containing disc flows into the liquid injection funnel, and the outer wall of the liquid injection funnel is in sealing connection with the mounting hole.
4. The animal experiment pathology specimen fixing, dehydrating and decalcification system according to claim 1, wherein: the end of the valve rod is hinged with the connecting rod so as to enable the valve rod to rotate relative to the connecting rod.
5. The animal experiment pathology specimen fixing, dehydrating and decalcification system according to claim 1, wherein: the side wall of the liquid discharge groove is also provided with a discharge thin pipe, and the discharge thin pipe is provided with a main valve.
6. A method for fixing, dehydrating and decalcifying an animal experimental pathological specimen by using the animal experimental pathological specimen fixing, dehydrating and decalcifying system as claimed in any one of claims 1 to 5, characterized by comprising the steps of:
s1, placing a specimen in a specimen tube filled with a soaking solution, and keeping the test for required time;
s2, when the soaking liquid needs to be replaced, removing a sealing cover of the opening of each sample tube, installing a liquid injection assembly above the fixed assembly, and keeping a liquid injection funnel of the liquid injection assembly above the sample tubes;
s3, opening a valve of a liquid discharge control mechanism, and discharging all used soaking liquid in the sample tubes at one time;
s4, closing a valve of the liquid discharge control mechanism;
s5, pouring new soaking liquid into the liquid injection tray, and filling each sample tube with the new soaking liquid;
and S6, removing the liquid injection assembly, covering the mouth of each specimen tube with a sealing cover, and keeping the experiment for required time.
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