CN221007599U

CN221007599U - Light-shielding mixing box for protein imprinting

Info

Publication number: CN221007599U
Application number: CN202322872351.9U
Authority: CN
Inventors: 吴剑慧; 段学章; 孙静; 李栋; 张丹; 赵晓芳
Original assignee: Fifth Medical Center of PLA General Hospital
Current assignee: Fifth Medical Center of PLA General Hospital
Priority date: 2023-10-25
Filing date: 2023-10-25
Publication date: 2024-05-24
Anticipated expiration: 2033-10-25

Abstract

The utility model provides a light-shielding mixing box for protein blotting, and belongs to the technical field of protein blotting experiments. The luminous box comprises a box body, a plurality of holding tank has been seted up to surface one side of box body, the cover is established on the holding tank and is connected with luminous liquid storage structure, the surface of box body is close to holding tank one side and has been seted up a plurality of and is corresponding mixed work groove with it, the mixing hole of mutual intercommunication has been seted up to the bottom of mixed work groove and holding tank, a side surface wall top fixedly connected with shading cover plate of mixed work groove, surface wall both sides fixedly connected with baffle in the mixed work groove. According to the utility model, the luminous liquid storage structure is arranged, the ECL luminous liquid which is prepared at present is stored in the storage tube, and the storage tube keeps the ECL luminous liquid from light until the ECL luminous liquid is mixed with the PVDF film after the preparation is finished, so that the condition that the internal components of the ECL luminous liquid lose activity due to light is avoided.

Description

Light-shielding mixing box for protein imprinting

Technical Field

The utility model relates to the technical field of Western blotting experiments, in particular to a light-shielding mixing box for Western blotting.

Background

Western blotting is a method for detecting a certain protein in a complex sample according to the specific binding of antigen and antibody, and is a novel immunochemical technology developed on the basis of gel electrophoresis and solid phase immunoassay technology, and is commonly used for identifying a certain protein and can perform qualitative and semi-quantitative analysis on the protein. By combining with chemiluminescence detection, the expression quantity difference of the homologous proteins of a plurality of samples can be simultaneously compared.

When the existing Western blotting experiment is performed in a color development exposure step, ECL luminous liquid is prepared and then needs to be stored in a dark place, then the ECL luminous liquid is mixed with a PVDF film in a laboratory, the ECL luminous liquid is exposed when being mixed with the PVDF film before the ECL luminous liquid is turned off, meanwhile, the PVDF film is easy to adhere to the bottom of a mixing tool and difficult to take out when being mixed, and workers can mix the ECL luminous liquid manually, so that the mixing effect is poor.

Disclosure of utility model

The utility model aims to solve the technical problems that when a color development and exposure step is carried out in the existing western blotting experiment, ECL luminous liquid needs to be stored in a dark place after being prepared, then the ECL luminous liquid is mixed with a PVDF film in a laboratory, the ECL luminous liquid is exposed when being mixed with the PVDF film before the light is turned off, and meanwhile, the bottom of a mixing tool is easy to adhere and difficult to take out when the PVDF film is mixed for work, and a worker manually mixes the ECL luminous liquid, so that the mixing effect is poor.

In order to solve the technical problems, the utility model provides the following technical scheme:

The utility model provides a light-resistant mixing box for protein imprinting, includes the box body, a plurality of holding tank has been seted up to surface one side of box body, the cover is established on the holding tank and is connected with luminous liquid storage structure, a plurality of mixed work groove that corresponds with it has been seted up near holding tank one side on the surface of box body, the mixing hole of mutual intercommunication has been seted up to the bottom of mixed work groove and holding tank, a side surface wall top fixedly connected with shading apron of mixed work groove, the interior table wall both sides fixedly connected with baffle of mixed work groove, light-resistant mixing groove and vibration groove have been seted up to the both sides that are close to the baffle inside the mixed work groove, vibration structure is fixedly connected with vibration structure inside the vibration groove, light-resistant mixing groove bottom surface fixedly connected with PVDF membrane fixed knot constructs, fixedly connected with between vibration structure and the PVDF membrane fixed knot constructs.

Preferably, the luminous liquid storage structure comprises a storage tube sleeved on the inner surface wall of the storage tank, a piston rod is sleeved on the inner surface wall of the storage tube, one end, close to the piston rod, of the storage tube is fixedly connected with a limiting ring, and a liquid outlet is formed in the other end of the storage tube.

Preferably, a limit groove matched with the limit ring is formed in the upper end of the inner surface wall of the storage groove, the diameter of the inner surface wall of the storage groove is consistent with that of the outer surface wall of the storage pipe, and the depth of the storage pipe is slightly smaller than that of the storage groove.

Preferably, the vibration structure comprises a vibration motor fixedly connected to one side surface of the partition plate, the output end of the vibration motor is fixedly connected with a vibration turntable, a vibration connecting rod is fixedly connected to the edge of one side surface of the vibration turntable, and one end of the vibration connecting rod, far away from the vibration turntable, penetrates through the partition plate and extends into the light-shielding mixing tank to be fixedly connected with the PVDF film fixing structure.

Preferably, the PVDF film fixing structure comprises four spring columns which are respectively fixed on two sides of the bottom surface of the light-shielding mixing tank, each two spring columns are fixedly connected with a PVDF film first placing rack, the upper surface of the PVDF film first placing rack is rotationally connected with a PVDF film second placing rack, two placing rack connecting rods are fixedly connected at the middle positions of the opposite surfaces of the PVDF film first placing racks, and vibration connecting rods are fixedly connected at the middle positions of the surfaces of the placing rack connecting rods, which are close to one side of the partition plate.

Preferably, the one ends of the first PVDF film placing frame and the second PVDF film placing frame are rotationally connected through a rotating hinge, a limiting rod is fixedly connected with the surface of one end, far away from the rotating hinge, of the spring column, a limiting groove is formed in the position, corresponding to the limiting rod, of one side surface of the second PVDF film placing frame, and the first PVDF film placing frame and the second PVDF film placing frame are of triangular structures with the same specification.

Preferably, the waste liquid collecting box is fixedly connected with the lower part of the box body, which is close to the mixed working groove, and the waste liquid groove is formed in the surface of one side of the waste liquid collecting box, the waste liquid draining hole is formed in the bottom surface of the mixed working groove, the waste liquid groove is communicated with the mixed working groove through the waste liquid draining hole, and the timer is fixedly connected with the surface of one side of the box body.

Preferably, the bottom surface of the mixing working tank is an arc-shaped surface, and the waste liquid tank is positioned at the bottom end of the arc-shaped surface.

Compared with the prior art, the utility model has at least the following beneficial effects:

In the scheme, through setting up luminous liquid storage structure, the ECL luminous liquid of present preparation is inside the storage tube, and the storage tube is when preserving ECL luminous liquid for ECL luminous liquid is after the completion of present preparation until mixing with PVDF membrane, keeps the light-proof always, avoids ECL luminous liquid internal component to lose the condition of activity because of light.

Through setting up PVDF membrane fixed knot constructs, fix the simple easy operation of PVDF membrane through the first rack of PVDF membrane and PVDF membrane second rack, the PVDF membrane can not paste the light-resistant mixed groove bottom surface when mixing with ECL luminous liquid, avoid the problem that the PVDF membrane is difficult to take out, and PVDF membrane fixed knot constructs and PVDF membrane area of contact is little, can not influence ECL luminous liquid and PVDF membrane contact and mix, shake the fixed knot of PVDF membrane through vibrating structure simultaneously, shake the mixture for the manual hand of staff, PVDF membrane and ECL luminous liquid mix more thoroughly for development imaging effect is better.

Drawings

The accompanying drawings, which are incorporated herein and form a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the disclosure and to enable a person skilled in the pertinent art to make and use the disclosure.

FIG. 1 is a schematic perspective view of a light-shielding mixing box for Western blotting;

Fig. 2 is a schematic perspective view of a light shielding cover plate in a light shielding mixing box for western blotting;

FIG. 3 is an enlarged perspective view of the interior of the hybrid tank;

FIG. 4 is a schematic view of an enlarged assembly structure of vibration connecting rods, vibration turntables and rack connecting rods;

FIG. 5 is a schematic view of a PVDF membrane fixing structure in a three-dimensional enlarged structure;

FIG. 6 is a schematic diagram of a three-dimensional enlarged structure of a luminescent liquid storage structure;

FIG. 7 is an enlarged perspective view of a piston rod;

FIG. 8 is an enlarged perspective view of a waste liquid collecting box.

[ Reference numerals ]

1. A case body; 11. a mixing working tank; 12. a mixing hole; 13. a partition plate; 14. a rack connecting rod; 141. a spring post; 142. a PVDF film first rack; 143. a PVDF film second placing rack; 144. a rotating hinge; 15. a vibration tank; 16. a vibration motor; 17. vibrating the connecting rod; 18. vibrating the turntable; 2. a light shielding cover plate; 3. a waste liquid collection box; 31. a waste liquid tank; 4. a storage tank; 5. a luminescent liquid storage structure; 51. a piston rod; 52. a storage tube; 53. and a liquid outlet.

While particular structures and devices are shown in the drawings to enable a clear implementation of embodiments of the utility model, this is for illustrative purposes only and is not intended to limit the utility model to the particular structures, devices and environments, which may be modified or adapted by those of ordinary skill in the art, as desired, and which remain within the scope of the appended claims.

Detailed Description

The light-shielding mixing box for protein blotting provided by the utility model is described in detail below with reference to the accompanying drawings and specific examples. While the utility model has been described herein in terms of the preferred and preferred embodiments, the following embodiments are intended to be more illustrative, and may be implemented in many alternative ways as will occur to those of skill in the art; and the accompanying drawings are only for the purpose of describing the embodiments more specifically and are not intended to limit the utility model specifically.

It should be noted that references in the specification to "one embodiment," "an example embodiment," "some embodiments," etc., indicate that the embodiment described may include a particular feature, structure, or characteristic, but every embodiment may not necessarily include the particular feature, structure, or characteristic. Further, when a particular feature, structure, or characteristic is described in connection with an embodiment, it is submitted that it is within the knowledge of one skilled in the relevant art to effect such feature, structure, or characteristic in connection with other embodiments whether or not explicitly described.

Generally, the terminology may be understood, at least in part, from the use of context. For example, the term "one or more" as used herein may be used to describe any feature, structure, or characteristic in a singular sense, or may be used to describe a combination of features, structures, or characteristics in a plural sense, depending at least in part on the context. In addition, the term "based on" may be understood as not necessarily intended to convey an exclusive set of factors, but may instead, depending at least in part on the context, allow for other factors that are not necessarily explicitly described.

It will be understood that the meanings of "on … …", "on … …" and "over … …" in this disclosure should be interpreted in the broadest sense so that "on … …" means not only "directly on" something but also includes the meaning of "on" something with intervening features or layers therebetween, and "on … …" or "over … …" means not only "on" or "over" something, but also may include its meaning of "on" or "over" something without intervening features or layers therebetween.

Furthermore, spatially relative terms such as "under …," "under …," "lower," "above …," "upper," and the like may be used herein for ease of description to describe one element or feature's relationship to another element or feature as illustrated in the figures. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. The device may be otherwise oriented and the spatially relative descriptors used herein may similarly be interpreted accordingly.

As shown in fig. 1 to 8, an embodiment of the present utility model provides a light-proof mixing box for western blotting, which comprises a box body 1, wherein a plurality of storage tanks 4 are provided on one side of the surface of the box body 1, a luminescent liquid storage structure 5 is sleeved on the storage tanks 4, a plurality of mixing working tanks 11 corresponding to the storage tanks are provided on one side of the surface of the box body 1, mixing holes 12 which are mutually communicated are provided at the bottoms of the mixing working tanks 11 and the storage tanks 4, a shading cover plate 2 is fixedly connected to the top end of one side surface wall of the mixing working tank 11, a partition plate 13 is fixedly connected to two sides of the inner surface wall of the mixing working tank 11, a light-proof mixing tank and a vibration tank 15 are provided on two sides, which are close to the partition plate 13, of the inside of the mixing working tank 11, a vibration structure is fixedly connected to the inside of the vibration tank 15, a PVDF film fixing structure is fixedly connected to the bottom surface of the light-proof mixing tank, and the PVDF film fixing structure are fixedly connected.

The luminous liquid storage structure 5 comprises a storage tube 52 sleeved on the inner surface wall of the storage tank 4, a piston rod 51 is sleeved on the inner surface wall of the storage tube 52, one end, close to the piston rod 51, of the storage tube 52 is fixedly connected with a limiting ring, and the other end is provided with a liquid outlet 53; when the final step of development exposure work of the Western blotting experiment is performed, the prepared ECL luminous liquid is poured into the storage tube 52 plugged by the liquid outlet 53 after being prepared, then the piston rod 51 is plugged into the storage tube 52, then the liquid outlet 53 is opened, the ECL luminous liquid cannot flow out of the liquid outlet 53 under the action of internal pressure because the piston rod 51 is plugged into the storage tube 52, the storage tube 52 with the ECL luminous liquid is placed on the storage tank 4, the ECL luminous liquid inside the storage tank 4 is preserved in a dark place because the piston rod 51 and the storage tube 52 are made of black opaque plastic materials, then the PVDF film carrier is placed on the PVDF film fixing structure, the light shielding cover plate 2 is closed, then the piston rod 51 is pressed to enable the ECL luminous liquid inside the storage tube 52 to flow into the dark place from the mixing hole 12 below the storage tank 4, so that the ECL luminous liquid entirely covers the PVDF film, opening the vibration structure in the vibration groove 15 to enable the vibration structure to drive the PVDF film fixing structure to vibrate, thereby completely mixing the PVDF film with the ECL working solution, placing the PVDF film on an imager for imaging after mixing, keeping ECL luminous solution through the luminous solution storage structure 5 until mixing with the PVDF film after finishing the preparation, keeping light-proof all the time, avoiding the condition that the internal components of the ECL luminous solution lose activity due to light, simultaneously fixing the PVDF film by the PVDF film fixing structure, being simple and easy to operate, the PVDF film can not be stuck on the bottom surface of the light-proof mixing groove when mixing with the ECL luminous solution, avoiding the problem that the PVDF film is difficult to take out, and the PVDF film fixing structure has small contact area with the PVDF film, does not influence the contact mixing of the ECL luminous solution with the PVDF film, simultaneously vibrating and shaking the PVDF film fixing structure through the vibration structure, and mixing manually relative to staff, PVDF film and ECL luminous solution are mixed more thoroughly, so that the developing imaging effect is better.

As shown in fig. 1 and 2, the upper end of the inner surface wall of the storage tank 4 is provided with a limit groove adapted to the limit ring, the diameter of the inner surface wall of the storage tank 4 is identical to the diameter of the outer surface wall of the storage tube 52, the depth of the storage tube 52 is slightly smaller than that of the storage tank 4, and when the storage light 52 is placed on the storage tank 4, the storage tube 52 is limited and placed through the limit ring and the limit groove, and meanwhile, the inside light-shielding effect of the storage tank 4 is better.

As shown in fig. 3 and fig. 4, the vibration structure comprises a vibration motor 16 fixedly connected to one side surface of the partition 13, a vibration turntable 18 fixedly connected to the output end of the vibration motor 16, a vibration connecting rod 17 fixedly connected to the edge of one side surface of the vibration turntable 18, and one end of the vibration connecting rod 17 away from the vibration turntable 18 extending through the partition 13 into a light-shielding mixing tank and fixedly connected with the PVDF film fixing structure; the vibration motor 16 rotates to drive the vibration turntable 18 to rotate to drive the vibration connecting rod 17 of connection in vibration turntable 18 edge to reciprocate, reciprocating vibration connecting rod 17 can pull rack connecting rod 14 to reciprocate, thereby drive PVDF membrane fixed knot constructs and reciprocate, make PVDF membrane fixed knot construct on the PVDF membrane shake in ECL luminous flux and mix, shake the mixture for the manpower, shake the work through vibration motor 16, when more laborsaving, the effect of mixing is also better.

As shown in fig. 4 and fig. 5, the PVDF film fixing structure includes four spring columns 141 respectively fixed on two sides of the bottom surface of the light-shielding mixing tank, a PVDF film first rack 142 is fixedly connected to each of the two spring columns 141, a PVDF film second rack 143 is rotatably connected to the upper surface of the PVDF film first rack 142, a rack connecting rod 14 is fixedly connected to the middle position of the opposite surfaces of the two PVDF film first racks 142, and a vibration connecting rod 17 is fixedly connected to the middle position of the surface of the rack connecting rod 14 near one side of the partition 13; the PVDF film first placing frame 142 is rotationally connected with one end of the PVDF film second placing frame 143 through the rotating hinge 144, a limiting rod is fixedly connected with the surface of one end, far away from the rotating hinge 144, of the spring column 141, a limiting groove is formed in the position, corresponding to the limiting rod, of one side surface of the PVDF film second placing frame 143, and the PVDF film first placing frame 142 and the PVDF film second placing frame 143 are of triangular structures with the same specification, so that the contact area between the PVDF film first placing frame and the PVDF film second placing frame 143 can be effectively reduced; the magnet that attracts each other is fixedly connected with opposite face of PVDF membrane first rack 142 and PVDF membrane second rack 143, opens PVDF membrane second rack 143, uses tweezers to place the PVDF membrane on PVDF membrane first rack 142, then rotates PVDF membrane second rack 143 and presses down the PVDF membrane, and the spacing groove inserts on the gag lever post simultaneously to fix PVDF membrane second rack 143, simultaneously the mutual attraction of magnet on PVDF membrane first rack 142 and the PVDF membrane second rack 143 extrudes the fixed PVDF membrane, and fixed PVDF membrane is simple easy operation.

As shown in fig. 8, a waste liquid collecting box 3 is fixedly connected to a lower part of one side surface of the box body 1, which is close to the mixing working tank 11, a waste liquid tank 31 is arranged on one side surface of the waste liquid collecting box 3, a waste liquid draining hole is arranged on the bottom surface of the mixing working tank 11, the waste liquid tank 31 and the mixing working tank 11 are communicated through the waste liquid draining hole, the bottom surface of the mixing working tank 11 is an arc-shaped surface, and the waste liquid tank 31 is positioned at the bottom end of the arc-shaped surface; the waste liquid after the PVDF film and the ECl luminous liquid are mixed is discharged into a waste liquid groove 31 on the waste liquid collecting box 3 through a waste liquid discharging hole, so that the waste liquid is convenient for workers to process; a timer is fixedly connected to one side surface of the box body 1, and mixing time of the PVDF film and the ECl luminous liquid can be accurately known through the timer.

According to the technical scheme provided by the utility model, the luminous liquid storage structure is arranged, the ECL luminous liquid which is prepared at present is stored in the storage tube, and the storage tube stores the ECL luminous liquid, so that the ECL luminous liquid is kept away from light until being mixed with the PVDF film after the preparation is finished, and the condition that the internal components of the ECL luminous liquid lose activity due to light is avoided.

The utility model is intended to cover any alternatives, modifications, equivalents, and variations that fall within the spirit and scope of the utility model. In the following description of preferred embodiments of the utility model, specific details are set forth in order to provide a thorough understanding of the utility model, and the utility model will be fully understood to those skilled in the art without such details. In other instances, well-known methods, procedures, flows, components, circuits, and the like have not been described in detail so as not to unnecessarily obscure aspects of the present utility model.

Those of ordinary skill in the art will appreciate that all or a portion of the steps in implementing the methods of the embodiments described above may be implemented by a program that instructs associated hardware, and the program may be stored on a computer readable storage medium, such as: ROM/RAM, magnetic disks, optical disks, etc.

The foregoing is merely a preferred embodiment of the present utility model and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present utility model, which are intended to be comprehended within the scope of the present utility model.

Claims

1. The utility model provides a light-resistant mixing box for protein imprinting, its characterized in that, includes the box body, a plurality of holding tank has been seted up to surface one side of box body, the cover is established and is connected with luminous liquid storage structure on the holding tank, a plurality of mixed work groove that corresponds with it has been seted up near holding tank one side on the surface of box body, the mixing hole of intercommunication each other has been seted up to the bottom of mixed work groove and holding tank, a side surface wall top fixedly connected with shading apron of mixed work groove, surface wall both sides fixedly connected with baffle in the mixed work groove, light-resistant mixing groove and vibration groove have been seted up to the both sides that are close to the baffle inside the mixed work groove, vibration groove inside fixedly connected with vibration structure, light-resistant mixing groove bottom surface fixedly connected with PVDF membrane fixed knot constructs, fixedly connected with between vibration structure and the PVDF membrane fixed knot constructs.

2. The light-resistant mixing box for western blotting according to claim 1, wherein the luminescent liquid storage structure comprises a storage tube sleeved on the inner surface wall of the storage tank, the inner surface wall of the storage tube is sleeved with a piston rod, one end, close to the piston rod, of the storage tube is fixedly connected with a limiting ring, and the other end of the storage tube is provided with a liquid outlet.

3. The light-resistant mixing box for western blot according to claim 2, wherein a limit groove matched with the limit ring is arranged at the upper end of the inner surface wall of the storage groove, the diameter of the inner surface wall of the storage groove is consistent with the diameter of the outer surface wall of the storage tube, and the depth of the storage tube is slightly smaller than that of the storage groove.

4. The light-resistant mixing box for western blotting according to claim 1, wherein the vibration structure comprises a vibration motor fixedly connected to one side surface of the partition plate, the output end of the vibration motor is fixedly connected with a vibration turntable, the edge of one side surface of the vibration turntable is fixedly connected with a vibration connecting rod, and one end of the vibration connecting rod, far away from the vibration turntable, penetrates through the partition plate and extends into the light-resistant mixing groove to be fixedly connected with the PVDF film fixing structure.

5. The light-resistant mixing box for western blotting according to claim 1, wherein the PVDF film fixing structure comprises four spring columns fixed on two sides of the bottom surface of the light-resistant mixing tank, each two spring columns are fixedly connected with a PVDF film first placing frame, the upper surface of the PVDF film first placing frame is rotatably connected with a PVDF film second placing frame, a placing frame connecting rod is fixedly connected with the middle position of the opposite surfaces of the two PVDF film first placing frames, and a vibration connecting rod is fixedly connected with the middle position of the surface of the placing frame connecting rod, which is close to the partition plate.

6. The light-resistant mixing box for western blotting of claim 5, wherein the first PVDF film placing frame and one end of the second PVDF film placing frame are rotatably connected through a rotary hinge, a limiting rod is fixedly connected to the surface of one end of the spring column, which is far away from the rotary hinge, a limiting groove is formed in the position, corresponding to the limiting rod, of one side surface of the second PVDF film placing frame, and the first PVDF film placing frame and the second PVDF film placing frame are of a triangle structure with the same specification.

7. The light-resistant mixing box for western blotting according to claim 1, wherein a waste liquid collecting box is fixedly connected to a lower part of a side surface of the box body, which is close to the mixing working tank, a waste liquid tank is arranged on a side surface of the waste liquid collecting box, a waste liquid draining hole is arranged on the bottom surface of the mixing working tank, the waste liquid tank and the mixing working tank are communicated through the waste liquid draining hole, and a timer is fixedly connected to a side surface of the box body.

8. The light-resistant mixing box for western blotting of claim 7, wherein the bottom surface of the mixing working tank is an arc-shaped surface, and the waste liquid tank is positioned at the bottom end of the arc-shaped surface.