CN108444990A - A kind of detection of veterinary drugs in food apparatus and method based on micro-fluidic paper chip - Google Patents
A kind of detection of veterinary drugs in food apparatus and method based on micro-fluidic paper chip Download PDFInfo
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- CN108444990A CN108444990A CN201810123269.7A CN201810123269A CN108444990A CN 108444990 A CN108444990 A CN 108444990A CN 201810123269 A CN201810123269 A CN 201810123269A CN 108444990 A CN108444990 A CN 108444990A
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- G—PHYSICS
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/62—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving urea
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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Abstract
The present invention discloses a kind of detection of veterinary drugs in food apparatus and method based on micro-fluidic paper chip in field of biological detection,By dilution control module,Prepare liquid control module,Image detection module and micro-fluidic paper chip fixed module composition,Dilution control module and prepare liquid control module are located in magazine,Micro-fluidic paper chip includes dilution and prepare liquid mixing module,Urea detection module,Chloramphenicol detection module aflatoxins detection module,Streptomysin detection module and detection waste collection module,Microcontroller drives imaging sensor to urea colour developing area,Chloramphenicol colour developing area,Aflatoxins colour developing area,Streptomysin colour developing area carries out image detection,Collected colour developing image is transferred to microcontroller to handle,Microcontroller compares colour developing image with built-in colour developing database,Obtain the result of residue of veterinary drug,It can judge current residue of veterinary drug type,Detect four kinds of residue of veterinary drug ingredients simultaneously,Without artificially adding chemical reagent,It is easy to use.
Description
Technical field
The invention belongs to field of biological detection, are related to a kind of dress being detected to residue of veterinary drug using micro-fluidic paper chip
It sets and method.
Background technology
Micro-fluidic refill chip technology develops on the basis of traditional microfluidic chip technology, and it is micro- to overcome tradition
The of high cost of fluidic chip technology, complex manufacturing process and manufacture craft require the shortcomings of high, have at low cost, processing simple and
The advantages that easy to carry, is applied to the fields such as clinical diagnosis, food quality control and environmental monitoring.In the detection of micro-fluidic chip
In method, the method that Photoelectric Detection and image detection can be used, wherein Photoelectric Detection are more convenient, but can only once detect miniflow
Control one of chip colour developing area, easily by ambient light interference and accuracy of detection it is not high, and image detecting method can be simultaneously to multiple
Color development area is detected, and can by certain filtering algorithm reduce ambient light interference to improve accuracy of detection, but
It is complicated for operation.Currently, the detection of veterinary drugs in food technology based on immunological method, if China Patent No. is CN200510026487.1
Document in the animal medicine residue detection device that proposes, the operating environment in darkroom cannot be provided, scanner need to be utilized to carry out ash
Degree detection, bulky and cannot carry, these deficiencies limit being widely used for detection of veterinary drugs in food device.
Invention content
The present invention be directed to be detected existing for current detection of veterinary drugs in food device, type is single, integration degree is low, operation
Complicated disadvantage, propose that a kind of high, detection type easy to operate, repeated is abundant and good portability based on micro-fluidic paper chip
The strong error correction of high throughput detection of veterinary drugs in food apparatus and method.
A kind of detection of veterinary drugs in food device based on micro-fluidic paper chip of the present invention the technical solution adopted is that：By dilution
Control module, prepare liquid control module, image detection module and micro-fluidic paper chip fixed module composition, dilution control module
It is located in magazine with prepare liquid control module, image detection module fixes the top surface for being located at box, and micro-fluidic paper chip is fixed
Module fixation is located on the bottom surface of box；Dilution control module is identical with the structure of prepare liquid control module, by stepping
Motor, connecting rod, liquid storage pipe, ball valve and mouth composition is oozed, concatenation ball valve and is vertically arranged up and down, horizontal step among liquid storage pipe
Stepper motor connects ball valve by connecting rod；Image detection module is by microcontroller and is separately connected the image sensing of microcontroller
Device, touch screen, microcontroller and light emitting diode composition；There is micro-fluidic paper chip fixed module plastics objective table, plastics to carry
It includes that dilution and prepare liquid mixing module, urea detection module, chlorine are mould to have micro-fluidic paper chip, micro-fluidic paper chip in object platform
Plain detection module aflatoxins detection module, streptomysin detection module and detection waste collection module, dilution are mixed with prepare liquid
Molding block is mixed by prepare liquid injection port, dilution injection port, dilution with prepare liquid mixed zone, dilution in prepare liquid logical
Hybrid channel forms under road, dilution and prepare liquid, is drop outlets to be measured, dilution sample introduction right over prepare liquid injection port
Mouthful surface be dilution drop outlets, prepare liquid injection port and dilution injection port connect simultaneously and connection dilution with it is to be measured
The input terminal of liquid mixed zone, dilution connect to connect dilution and mix in prepare liquid with the output end of prepare liquid mixed zone all the way to be led to
The input terminal in road, another way connection dilution and hybrid channel under prepare liquid, the output of dilution and hybrid channel in prepare liquid
End is separately connected the input terminal and chloramphenicol monoclonal antibody immunity gold colloid of urea detection module mixed zone through a microchannel
The output end of the input terminal of bonding pad, urea detection module mixed zone connects urea detection module, and chloramphenicol monoclonal antibody is exempted from
The output end of epidemic disease gold colloid bonding pad connects chloramphenicol detection module behind chloramphenicol detection module mixed zone.Dilution with wait for
Survey liquid under hybrid channel output end through another microchannel be separately connected fluorescein mixed mark object bonding pad input terminal and
The input terminal of streptomysin detection module mixed zone, the output end of fluorescein mixed mark object bonding pad is through aflatoxins detection module
Mixed zone connects aflatoxins detection module, and the output end of streptomysin detection module mixed zone connects through tropaeolin mixture bonding pad
Chain link mycin detection module, streptomysin detection module, aflatoxins detection module, chloramphenicol detection module, urea detection module
Output end be all connected with discharging of waste liquid area.
The detection of the detection of veterinary drugs in food device is the technical solution adopted is that successively as follows：
Step 1：The concentration of hybrid detection liquid is set by touch screen and is preserved in the microcontroller, microcontroller is examined according to mixing
The concentration of survey liquid calculates required dilution and detects the dosage of liquid, two stepper motors is driven to rotate two ball valves of adjustment
Openings of sizes so control outflow dilution and detect liquid dosage, dilution by dilute drop outlets be flowed into dilution into
In sample mouth, prepare liquid is flowed by drop outlets to be measured in prepare liquid injection port；
Step 2：Dilution and prepare liquid first flow into dilution and are uniformly mixed with testing liquid mixed zone, then are flowed into simultaneously
Dilution is shunted with hybrid channel in prepare liquid and dilution with hybrid channel under prepare liquid, is flowed into urea simultaneously later
Detection module, chloramphenicol detection module, aflatoxins detection module, streptomysin detection module carry out chromogenic reaction；
Step 3：Microcontroller drives imaging sensor to urea colour developing area, chloramphenicol colour developing area, aflatoxins colour developing area, strepto-
Element colour developing area carries out image detection, and collected colour developing image is transferred to microcontroller and is handled, and microcontroller will develop the color
Image is compared with built-in colour developing database, obtains the result of residue of veterinary drug.
The present invention has the following advantages that compared with existing methods and techniques：（1）The present invention is using imaging sensor to colour developing
Region is positioned and is developed the color the acquisition of information, and the colour developing database in collected colour developing data and microprocessor is carried out pair
Than intelligent decision goes out current residue of veterinary drug type, can detect four kinds of residue of veterinary drug ingredients simultaneously, be widely used, universality
By force.
（2）The present invention is shown and is operated by touch screen using microcontroller as control core, has good people
Machine interacts.
（3）Carrier of the present invention using micro-fluidic paper chip as reaction, flowed and mixed using microchannel realization liquid,
It is small and at low cost.The various chemical materials needed for reaction are fixed using micro-fluidic paper chip, without artificial addition chemistry examination
Agent, it is easy to use.
（4）The color development area of micro-fluidic paper chip is designed rectangular and circular pattern by the present invention, has both facilitated colour developing number
According to the foundation in library, and substantially increase the accuracy of image recognition.
（5）Detection device of the present invention is placed in black acrylic board box, and light source is provided by light emitting diode,
Greatly reduce interference of the ambient light to image detection.
（6）Detection device volume of the present invention is small and exquisite, good portability, and dosage is few needed for detection, high degree of automation.
Description of the drawings
Fig. 1 present invention is a kind of overall structure signal of detection of veterinary drugs in food device based on micro-fluidic paper chip of the present invention
Figure；
Fig. 2 is the mounting structure right view of the dilution control module 100 and prepare liquid control module 101 in Fig. 1；
Fig. 3 is the mounting structure schematic diagram of the image detection module 102 in Fig. 1；
Fig. 4 is the structural schematic diagram of the micro-fluidic paper chip fixed module 103 in Fig. 1；
Fig. 5 is the plan structure enlarged diagram of the micro-fluidic paper chip 19 in Fig. 4；
Fig. 6 is the connection structure enlarged diagram of urea detection module 105 in Fig. 5；
Fig. 7 is the connection structure enlarged diagram of chloramphenicol detection module 106 in Fig. 5；
Fig. 8 is the connection structure enlarged diagram of aflatoxins detection module 108 in Fig. 5；
Fig. 9 is the connection structure enlarged diagram of Fig. 5 streptomycins detection module 109；
The overhaul flow chart of Figure 10 detection of veterinary drugs in food devices of the present invention.
The serial number and title of each component in attached drawing：
1. diluting drop outlets；2. the first stepper motor；3. drop outlets to be measured；4. head rod；5. the first ball valve；6. dilute
Release liquid liquid storage pipe；7. the second ball valve；8. prepare liquid liquid storage pipe；9. geometrical clamp；10. the second connecting rod；11. the second stepper motor；
13. imaging sensor；14. touch screen；15. microcontroller；16. light emitting diode；17. box；18. micro-fluidic paper chip is fixed
Box；19. micro-fluidic paper chip；20. prepare liquid injection port；21. dilution injection port；34. plastics objective table；35. card slot；36.
Square fixed block；38. dilution and testing liquid mixed zone；39. dilution and hybrid channel in prepare liquid；40. chloramphenicol list
Gold colloid bonding pad is immunized in clonal antibody；41. chloramphenicol detection module mixed zone；42. urea detection module mixed zone；47. micro-
Channel；48. discharging of waste liquid area；53. tropaeolin mixture bonding pad；54. streptomysin detection module mixed zone；55. aflatoxins
Detection module mixed zone；56. fluorescein mixed mark object bonding pad；57. dilution and hybrid channel under prepare liquid；58. urase
Detect material；59. blank control material；60. urase detects material；61. blank control material；62. urase detects material；63.
Blank control material；64. chloramphenicol matter loses line；65. chloramphenicol matter loses line；66. chloramphenicol matter loses line；67. chloramphenicol detection line；
68. chloramphenicol detection line；69. chloramphenicol detection line；70. aspergillus flavus quality loses line；71. aspergillus flavus quality loses line；72. aspergillus flavus
Quality loses line；73. aflatoxins detection line；74. aflatoxins detection line；75. aflatoxins detection line；76. smearing TPL solution
Material part；77. blank control material part；78. smearing TPL solution materials part；79. blank control material part；80. applying
Smear TPL solution materials part；81. blank control material part；100. dilution control module；101. prepare liquid control module；
102. image detection module；103. micro-fluidic paper chip fixed module；104. dilution and prepare liquid mixing module；105. urea
Detection module；106. chloramphenicol detection module；108. aflatoxins detection module；109. streptomysin detection module；110. urea
Develop the color area；The area 111. chloramphenicol develops the color；The area 112. aflatoxins develops the color；The area 113. streptomysin develops the color.
Specific implementation mode
Referring to Fig. 1, detection of veterinary drugs in food device of the present invention is mainly by dilution control module 100, prepare liquid
This 4 block combiners of control module 101, image detection module 102 and micro-fluidic paper chip fixed module 103 form.It is wherein dilute
It releases liquid control module 100 and prepare liquid control module 101 is fixedly mounted on the left side of box 17, image detection module 102 is fixed
On the top surface outer wall of box 17.Box 17 is magazine rectangular made of black acrylic board, and micro-fluidic paper chip is solid
Cover half block 103 is fixedly mounted on the bottom surface inner wall of box 17, and micro-fluidic paper chip fixed module 10 is located at dilution hydraulic control
The lower section of molding block 100 and prepare liquid control module 101.
Referring to Fig. 2, dilution control module 100 is by the first stepper motor 2, head rod 4, dilution liquid storage pipe 6,
One ball valve 5 and dilution drop outlets 1 this five components compositions.Prepare liquid control module 101 is mainly by the second stepper motor 11,
This five component compositions of two connecting rods 10, prepare liquid liquid storage pipe 8, the second ball valve 7 and drop outlets to be measured 3.Dilution controls mould
Block 100 is arranged symmetrically before and after identical with the structure of prepare liquid control module 101 and left side inner wall center relative to box 17.
It is illustrated by taking dilution control module 100 as an example：First stepper motor, 2 anterior-posterior horizontal is arranged, and is located inside box 17, fixed
On the leading flank of box 17.About 6 dilution liquid storage pipe is vertically arranged, and 6 hypomere of dilution liquid storage pipe is hung down by 17 top surface of box
Straight to stretch into inside, the epimere and hypomere of dilution liquid storage pipe 6 respectively screw in a geometrical clamp 9, box 17 are fixed on by geometrical clamp 9
On, wherein the geometrical clamp 9 of top is fixed by two mounting holes on 17 top surface of box, and the geometrical clamp 9 of lower section is by 17 left side of box
Two mounting holes on wall are fixed.The interlude of dilution liquid storage pipe 6 has concatenated the first ball valve 5, and the first ball valve 5 also is located at box
Inside 17, the first ball valve 5 is fixedly connected on one end of the head rod 4 of anterior-posterior horizontal arrangement, the other end of head rod 4
It is fixedly and coaxially connected the output shaft of the first stepper motor 2, the rotation of the first stepper motor 2 drives the rotation of the first ball valve 5, passes through first
The rotation of ball valve 5 reaches the mesh for controlling dilution outflow dosage to control the dilution dosage that dilution liquid storage pipe 6 flows downward out
's.The bottom end of dilution liquid storage pipe 6 is dilution drop outlets 1, and dilution drop outlets 1 are cones, realize the uniform of dilution
It oozes.The second stepper motor 11 in prepare liquid control module 101 is identical as the structure of the first stepper motor 2 and be arranged symmetrically,
Second ball valve 7 is identical as 5 structure of the first ball valve and be arranged symmetrically, prepare liquid liquid storage pipe 8 it is identical as the structure of dilution liquid storage pipe 6
And be arranged symmetrically Face to face, the second stepper motor 11 connects the second ball valve 7, the second stepper motor 11 by the second connecting rod 10
Rotation drives the second ball valve 7, and the prepare liquid dosage that prepare liquid liquid storage pipe 8 flows downward out is controlled by the rotation of the second ball valve 7,
The bottom end of prepare liquid liquid storage pipe 8 is drop outlets 3 to be measured, and prepare liquid outlet 3 is cone, realizes uniformly oozing for prepare liquid.
Referring to Fig. 3, image detection module 102 is by imaging sensor 13, touch screen 14, microcontroller 15 and light emitting diode
16 this four part form.Imaging sensor 13, touch screen 14 connect microcontroller by control line respectively with light emitting diode 16
15.The camera lens of imaging sensor 13 is inserted vertically by the opening at 17 top of box inside box 17, and is fixed on the top of box 17
On face, to complete the fixation of imaging sensor 13, imaging sensor 13 can take micro-fluidic paper chip stent below
19 region of micro-fluidic paper chip in block 103.Touch screen 14 and microcontroller 15 are consolidated by mounting hole and 17 top surface of box
Fixed, light emitting diode 16 is inverted to be inserted into inside box 17 by 17 top surface of box and is fixed.When carrying out image detection, by sending out
Optical diode 16 provides light source, since detection carries out in box 17, greatly reduces the influence of ambient light, imaging sensor 13
Colour developing situation in the captured region of detection, and colour developing real-time data transmission is analyzed to microcontroller 15, by microcontroller
Device 15 to colour developing data analyze and determine and compare with built-in colour developing database, includes finally touching by testing result
It touches on screen 14.
Referring to Fig. 3 and Fig. 4, micro-fluidic 103 fixed box of paper chip fixed module, 17 bottom, micro-fluidic paper chip is fixed
It is the square fixed block 36 being made of transparent plastic outside module 103, is plastics objective table 34 inside square fixed block 36, it is rectangular
Card slot 35 is provided on the side wall of fixed block 36, plastics objective table 34 is connect by card slot 35 with square fixed block 36, plastics loading
Platform 34 can be realized along card slot 35 moves push-and-pull.It placed micro-fluidic paper chip fixed bin 18 on 34 upper surface of plastics objective table,
Micro-fluidic paper chip 19 is placed in micro-fluidic paper chip fixed bin 18, micro-fluidic paper is realized by slide plastic objective table 34
The taking-up and replacement of chip 19.When replacing micro-fluidic paper chip 19, plastics objective table 34 need to only be pulled out, by plastics loading
Micro-fluidic paper chip fixed bin 18 on platform 34 takes out, then new micro-fluidic paper chip 19 is placed on micro-fluidic paper chip and is fixed
On box 18, finally micro-fluidic paper chip fixed bin 18 and plastics objective table 34 are rolled back, so far complete micro-fluidic paper chip
19 replacement.
Referring to Fig. 5, micro-fluidic paper chip 19 include dilution and prepare liquid mixing module 104, urea detection module 105,
Chloramphenicol detection module 106, aflatoxins detection module 108, streptomysin detection module 109 and detection waste collection module 107.
Wherein, dilution and prepare liquid mixing module 104 are located at the leftward position of micro-fluidic paper chip 19, by prepare liquid injection port 20,
Hybrid channel 39 in dilution injection port 21, dilution and prepare liquid mixed zone 38, dilution and prepare liquid, dilution with it is to be measured
The part of hybrid channel 57 this 5 forms under liquid.The surface of prepare liquid injection port 20 is the drop outlets to be measured 3 in Fig. 2, to be measured
Liquid is added dropwise into prepare liquid injection port 20 downwards from drop outlets 3 to be measured, and the surface of dilution injection port 21 is the dilution in Fig. 2
Drop outlets 1, dilution are added dropwise into dilution injection port 21 downwards from dilution drop outlets 1.Prepare liquid injection port 20 and dilution
Liquid injection port 21 connects and is connected to simultaneously the input terminal of dilution and prepare liquid mixed zone 38, dilution and prepare liquid mixed zone 38
Output end divide two-way, connect dilution all the way and connect dilution and prepare liquid with hybrid channel in prepare liquid 39, another way
The input terminal of lower hybrid channel 57.Dilution and the output end of hybrid channel in prepare liquid 39 are separately connected through a microchannel 47
The input terminal of the input terminal and chloramphenicol monoclonal antibody immunity gold colloid bonding pad 40 of urea detection module mixed zone 42, urea
The output end connection urea detection module 105 of detection module mixed zone 42, chloramphenicol monoclonal antibody immunity gold colloid bonding pad
40 output end connects chloramphenicol detection module 106 behind chloramphenicol detection module mixed zone 41.Under dilution and prepare liquid
The output end of hybrid channel 57 through another microchannel 47 be separately connected fluorescein mixed mark object bonding pad 56 input terminal and
The output end of the input terminal of streptomysin detection module mixed zone 54, fluorescein mixed mark object bonding pad 56 is detected through aflatoxins
Module mixed zone 55 connects aflatoxins detection module 108, and the output end of streptomysin detection module mixed zone 54 is mixed through tropaeolin
It closes object bonding pad 53 and connects streptomysin detection module 109.Streptomysin detection module 109, aflatoxins detection module 108, chlorine are mould
Plain detection module 106, urea detection module 105 output end be all connected with discharging of waste liquid area 48.Discharging of waste liquid area 48 is by large area
Water-absorbent material constitute, for absorbs flow into detection waste liquid, avoid detection waste liquid to colour developing result influence.
Referring to Fig. 6, urea detection module 105 has urea colour developing area 110, and urea colour developing area 110 is border circular areas, urea
The border circular areas division in colour developing area 110 has 6 fan-shaped detection zones, is 3 urase material tests parts and 3 blank controls respectively
Material part, i.e. urase material tests part 58,60,62 and blank control material part 59,61,63, each urase material inspection
It surveys part and blank control material part is interval-staggered, realize the colour developing to urea.It is mixed from dilution in prepare liquid
The detection liquid after evenly mixing that channel 39 exports first is mixed in urea detection module mixed zone 42, after to be flowed into urea aobvious
Chromogenic reaction is carried out in color area 110.Wherein, urase detection material part 58 and urase detection material part 60 are displayed in red, and
Urase detection material part 62 does not show any color, and blank control material part 59,61 and 63 is without colour developing.By to colour developing
Situation is judged, can be shown that urea without rotten, is contained in detection liquid in entire urea colour developing area 110.Waste liquor stream after detection
Go out to discharging of waste liquid area 48.
Referring to Fig. 7, chloramphenicol detection module 106 has chloramphenicol colour developing area 111, and chloramphenicol colour developing area 111 is rectangle region
Domain, chloramphenicol develop the color area 111 rectangular area by 3 hough transform district's groups arranged side by side at each hough transform region all has
A matter being arranged symmetrically loses line and a detection line, i.e., the side in each hough transform area is that chloramphenicol matter loses line, the other side
Chloramphenicol detection line, i.e. chloramphenicol matter in 3 hough transform areas lose line be respectively chloramphenicol matter lose line 64,65,66, chlorine it is mould
Plain detection line is chloramphenicol detection line 67,68,69 respectively.A matter in each hough transform region loses line and a detection line
Realize the colour developing to chloramphenicol.It is first flowed into from dilution and the detection liquid after evenly mixing that hybrid channel in prepare liquid 39 exports
To chloramphenicol monoclonal antibody immunity gold colloid bonding pad 40, it is allowed to and chloramphenicol monoclonal antibody immunity gold colloid bonding pad 40
On gel phase combine, then mixed in chloramphenicol detection module mixed zone 41, after be flowed into chloramphenicol colour developing area 111
Carry out chromogenic reaction.Wherein, the chloramphenicol matter in 3 hough transform areas loses line 64,65,66 and all shows red line, 3 rectangle inspections
The chloramphenicol detection line for surveying area only has chloramphenicol detection line 69 to show red line, and two other chloramphenicol detection line 67,68
It is not displayed in red line, by judging colour developing situation, can be shown that entire chloramphenicol colour developing area 111 without going bad, is detected
Chloramphenicol is not contained in liquid.Waste liquid after detection flows out to discharging of waste liquid area 48.
Referring to Fig. 8, there is aflatoxins detection module 108 aflatoxins colour developing area 112, aflatoxins colour developing area 112 to be
Rectangular area, by 3 hough transform district's groups arranged side by side at there is a matter being arranged symmetrically to lose line and one in each hough transform area
Detection line, i.e. side are that aspergillus flavus quality loses line, the other side is aflatoxins detection line, and the Huang in 3 hough transform areas is bent
It is that aspergillus flavus quality loses line 70,71,72 respectively that mycin matter, which loses line, and the aflatoxins detection line in 3 hough transform areas is respectively
Aflatoxins detection line 73,74,75 realizes the colour developing to aflatoxins.It is exported from hybrid channel 57 under dilution and prepare liquid
Detection liquid after evenly mixing first flow into fluorescein mixed mark object bonding pad 56, be allowed to and fluorescein mixed mark object knot
The fluorescein mixed mark object closed on pad 56 is combined, and is mixed further along aflatoxins detection module mixed zone 55, rear to flow
Enter in developing the color area 112 to aflatoxins and carries out chromogenic reaction.The aspergillus flavus quality in 3 hough transform areas is lost line 70,71,72 and is not had
Show red line, aflatoxins detection line 73,74 shows red line, and an other aflatoxins detection line 75 is not shown
Red line.By judging colour developing situation, it can be shown that entire aflatoxins colour developing area 112 has gone bad, can not judge to examine
It surveys in liquid and whether contains aflatoxins.Waste liquid after detection flows out to discharging of waste liquid area 48.
Referring to Fig. 9, streptomysin detection module 109 has streptomysin colour developing area 113, and streptomysin colour developing area 113 is circle
Domain, border circular areas, which along the circumferential direction divides, 6 fan-shaped detection zones, be respectively 3 smear TPL solution materials detection parts and
3 blank control material parts, that is, smear TPL solution materials detection part 76,78,80 and blank control material part 77,79,
81, each smearing TPL solution materials detection part and blank control material part are interval-staggered, and realization shows streptomysin
Color.The detection liquid after evenly mixing exported from hybrid channel 57 under dilution and prepare liquid is first mixed along streptomysin detection module
Close area 54 to be mixed, then be flowed into tropaeolin mixture bonding pad 53, be allowed to above tropaeolin mixture bonding pad 53
Tropaeolin mixture is combined, after be flowed into streptomysin colour developing area 113 in carry out chromogenic reaction, wherein smear TPL solution materials
Part 76,78,80 is without colour developing, and blank control material part 77,81 shows red, and other blank control material portion
Divide 79 not develop the color, by judging colour developing situation, can be shown that entire streptomysin colour developing area 113 has gone bad, can not sentence
Whether contain streptomysin in disconnected detection liquid.Waste liquid after detection flows out to discharging of waste liquid area 48.
Referring to Fig. 1-10, detection process when detection of veterinary drugs in food device of the present invention works is：
Step 1：The plastics objective table 34 of micro-fluidic paper chip fixed module 103 is pulled out, micro-fluidic paper chip 19 is placed
On plastics objective table 34, finally plastics objective table 34 is rolled back, completes the fixation of micro-fluidic paper chip 19.
Step 2：Dilution is added dropwise in dilution liquid storage pipe 6 using pipette, prepare liquid is added dropwise to prepare liquid storage
In liquid pipe 8, the dropwise addition of dilution and prepare liquid is completed.
Step 3：The concentration of hybrid detection liquid is set by touch screen 14 and is stored in microcontroller 15.
Step 4：Microcontroller 15 calculates required dilution and detects the dosage of liquid according to the concentration of hybrid detection liquid,
Drive the rotation of the first stepper motor 2 drive the rotation of head rod 4 so as to adjust the openings of sizes of the first ball valve 5 into
And control outflow dilution dosage, dilution due to gravity by dilute drop outlets 1 be flowed into micro-fluidic paper chip
In 19 dilution injection port 21.Simultaneously drive the second stepper motor 11 rotation come drive the rotation of the second connecting rod 10 so as to
To adjust the openings of sizes of the second ball valve 7 and then control the dosage of outflow prepare liquid, prepare liquid is to be measured since gravity passes through
Drop outlets 3 are flowed into the prepare liquid injection port 20 of micro-fluidic paper chip 19, to complete the addition of prepare liquid.So far, it completes
The dilution of micro-fluidic paper chip 19 and the addition of prepare liquid.
Step 5：It is flowed into the dilution and prepare liquid of micro-fluidic paper chip 19, dilution is first flowed into and is mixed with testing liquid
Area 38 is uniformly mixed, then is flowed into dilution simultaneously and is mixed under hybrid channel in prepare liquid 39 and dilution and prepare liquid
Channel 57 is shunted, and is flowed into urea detection module 105, chloramphenicol detection module 106, aflatoxins detection mould simultaneously later
Block 108, streptomysin detection module 109 carry out chromogenic reaction.Wherein the developing time of urea is most about 2 minutes short, aflatoxins
Developing time take second place about 8 minutes, the developing time of streptomysin is slightly about 12 minutes long, and the developing time longest of chloramphenicol needs
Want 15 minutes.Microcontroller 15 is built-in by urea colour developing area 110, chloramphenicol colour developing area 111, aflatoxins colour developing area 112, chain
The colour developing database of all possible colour developing pattern composition in colour developing area of this four, mycin colour developing area 113.Pass through light emitting diode 16
Detection light source is provided, drives imaging sensor 13 mould to the urea colour developing area 110 of micro-fluidic paper chip 19, chlorine by microcontroller 15
Element colour developing area 111, aflatoxins colour developing area 112, streptomysin colour developing area 113 carry out image detection, if there is detection liquid to be flowed into phase
Timing is begun to behind the colour developing area answered, after corresponding developing time reaches, image detection just is carried out to corresponding colour developing area, it will
13 collected colour developing image of imaging sensor is transferred to microcontroller 15 and is handled, by microcontroller 15 will develop the color image with
Built-in colour developing database is compared, so as to quickly obtain corresponding residue of veterinary drug as a result, realizing residual to same veterinary drug
The repeatability detection three times stayed, solves the false positive issue of detection of veterinary drugs in food, substantially increases serious forgiveness well, it is ensured that
The objective and accurate property of testing result.It all detects and finishes in urea, chloramphenicol, aflatoxins, streptomysin these four residues of veterinary drug
Terminate the comparison of Image Acquisition and the pattern that develops the color afterwards.
Step 6：After the completion of four kinds of chromogenic reactions, urea, chloramphenicol, aflatoxins, chain that microcontroller 15 is obtained
The testing result of these four residues of veterinary drug of mycin is shown on touchscreen 14.
Claims (8)
1. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip, by dilution control module（100）, prepare liquid control
Module（101）, image detection module（102）With micro-fluidic paper chip fixed module（103）Composition, it is characterized in that：Dilute hydraulic control
Molding block（100）With prepare liquid control module（101）It is located at magazine（17）It is interior, image detection module（102）Fixation is located at box
Son（17）Top surface, micro-fluidic paper chip fixed module（103）Fixation is located in box（17）Bottom surface on；Dilution controls mould
Block（100）With prepare liquid control module（101）Structure it is identical, by stepper motor, connecting rod, liquid storage pipe, ball valve and ooze
Mouth composition, liquid storage pipe centre concatenate ball valve and are vertically arranged up and down, and horizontal stepper motor connects ball valve by connecting rod；Image
Detection module（102）By microcontroller（15）And it is separately connected microcontroller（15）Imaging sensor（13）, touch screen
（14）, microcontroller 15 and light emitting diode（16）Composition；Micro-fluidic paper chip fixed module 103 has plastics objective table 34,
There is micro-fluidic paper chip in plastics objective table 34（19）, micro-fluidic paper chip（19）Including dilution and prepare liquid mixing module
（104）, urea detection module（105）, chloramphenicol detection module（106）, aflatoxins detection module（108）, streptomysin detection
Module（109）With detection waste collection module（107）, dilution and prepare liquid mixing module（104）By prepare liquid injection port
（20）, dilution injection port（21）, dilution and prepare liquid mixed zone（38）, hybrid channel in dilution and prepare liquid（39）、
Dilution and hybrid channel under prepare liquid（57）Composition, prepare liquid injection port（20）Surface be drop outlets to be measured（3）, dilute
Release liquid injection port（21）Surface be dilution drop outlets（1）, prepare liquid injection port（20）With dilution injection port（21）Together
When connect and connection dilution and prepare liquid mixed zone（38）Input terminal, dilution and prepare liquid mixed zone（38）Output end
Dilution and hybrid channel in prepare liquid are connect all the way（39）Another way connects dilution and hybrid channel under prepare liquid（57）
Input terminal, hybrid channel in dilution and prepare liquid（39）Output end through a microchannel（47）It is separately connected urea detection
Module mixed zone（42）Input terminal and chloramphenicol monoclonal antibody immunity gold colloid bonding pad（40）Input terminal, urea detection
Module mixed zone（42）Output end connect urea detection module（105）, chloramphenicol monoclonal antibody immunity gold colloid bonding pad
（40）Output end pass through chloramphenicol detection module mixed zone（41）Chloramphenicol detection module is connected afterwards（106）；Dilution with wait for
Survey hybrid channel under liquid（57）Output end through another microchannel（47）It is separately connected fluorescein mixed mark object bonding pad 56
Input terminal and streptomysin detection module mixed zone（54）Input terminal, fluorescein mixed mark object bonding pad（56）Output end
Through aflatoxins detection module mixed zone（55）Connect aflatoxins detection module（108）, streptomysin detection module mixed zone
（54）Output end through tropaeolin mixture bonding pad（53）Connect streptomysin detection module（109）, streptomysin detection module
（109）, aflatoxins detection module（108）, chloramphenicol detection module（106）, urea detection module（105）Output end connect
Meet discharging of waste liquid area（48）.
2. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip according to claim 1, it is characterized in that：Urea
Detection module（105）Urea colour developing area with border circular areas（110）, border circular areas, which divides, 3 urase material tests parts
With 3 fan-shaped detection zones of blank control material part 6, each urase material tests part and blank control material part interval
Interlaced arrangement.
3. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip according to claim 1, it is characterized in that：Chlorine is mould
Plain detection module（106）Chloramphenicol colour developing area with rectangular area（111）, rectangular area is by 3 hough transform areas arranged side by side
Composition, each hough transform region all have a chloramphenicol matter being arranged symmetrically and lose line and a chloramphenicol detection line.
4. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip according to claim 1, it is characterized in that：Huang Qu
Mycin detection module（108）With 3 hough transform district's groups arranged side by side at each hough transform area has one be arranged symmetrically
Aspergillus flavus quality loses line and an aflatoxins detection line.
5. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip according to claim 1, it is characterized in that：Strepto-
Plain detection module（109）Streptomysin colour developing area with border circular areas（113）, border circular areas, which along the circumferential direction divides, 6 fans
The detection zone of shape is respectively 3 and smears TPL solution materials detection part and 3 blank control material parts, each smears TPL
Solution material detection part and blank control material part are interval-staggered.
6. a kind of detection of veterinary drugs in food device based on micro-fluidic paper chip according to claim 1, it is characterized in that：Strepto-
Plain detection module（109）, aflatoxins detection module（108）, chloramphenicol detection module（106）, urea detection module（105）'s
Output end is all connected with discharging of waste liquid area（48）.
7. a kind of detection method of the detection of veterinary drugs in food device based on micro-fluidic paper chip as described in claim 1, special
Sign is successively as follows：
Step 1：The concentration of hybrid detection liquid is set by touch screen 14 and is stored in microcontroller（15）In, microcontroller（15）
It according to the concentration of hybrid detection liquid, calculates required dilution and detects the dosage of liquid, drive two stepper motor rotation adjustment
The openings of sizes of two ball valves and then the dosage for controlling outflow dilution and detection liquid, dilution is by diluting drop outlets（1）
It is flowed into dilution injection port（21）In, prepare liquid passes through drop outlets to be measured（3）It is flowed into prepare liquid injection port（20）In；
Step 2：Dilution and prepare liquid first flow into dilution and testing liquid mixed zone（38）It is uniformly mixed, then is flowed simultaneously
Enter to hybrid channel on dilution and prepare liquid（39）With hybrid channel under dilution and prepare liquid（57）It is shunted, Zhi Houtong
When be flowed into urea detection module（105）, chloramphenicol detection module（106）, aflatoxins detection module（108）, streptomysin inspection
Survey module（109）Carry out chromogenic reaction；
Step 3：Microcontroller（15）Drive imaging sensor（13）To urea colour developing area（110）, chloramphenicol develop the color area（111）、
Aflatoxins colour developing area（112）, streptomysin develop the color area（113）Image detection is carried out, collected colour developing image is transferred to micro-
Controller（15）It is handled, microcontroller（15）Colour developing image is compared with built-in colour developing database, obtains veterinary drug
Remaining result.
8. detection method according to claim 7, it is characterized in that：In step 2, the developing time of urea is 2 minutes, Huang Qu
The developing time of mycin is 8 minutes, and the developing time of streptomysin is 12 minutes, and the developing time of chloramphenicol is 15 minutes.
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