CN208187823U - A kind of dyeing apparatus - Google Patents
A kind of dyeing apparatus Download PDFInfo
- Publication number
- CN208187823U CN208187823U CN201820397806.2U CN201820397806U CN208187823U CN 208187823 U CN208187823 U CN 208187823U CN 201820397806 U CN201820397806 U CN 201820397806U CN 208187823 U CN208187823 U CN 208187823U
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- China
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- dyeing apparatus
- liquid
- cylinder
- cover board
- counterdie
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Abstract
The utility model relates to medical biotechnology experimental provision technical fields, more particularly to a kind of dyeing apparatus, it includes pedestal and cover board, wherein, several liquid holding grooves are offered on the pedestal, the lower end surface of the cover board is provided with several cylinders, and the open end of the cylinder is covered with counterdie, several water seepage holes are offered on the counterdie；When the cover plate lid is set on the pedestal, the cylinder and the liquid holding groove are corresponded, and the cylinder is inserted in the liquid holding groove.In the dyeing course of histotomy, when needing replacing liquid, the liquid in liquid holding groove can be replaced by removing cover board, residual liquid can be discharged by the water seepage hole on counterdie in cylinder simultaneously, eliminate the process of artificial sucking liquid, the problem of time and manpower is greatly saved, and also avoids the slice of the injury tissue as caused by artificial sucking liquid, effectively reduces the loss late of histotomy.
Description
Technical field
The utility model relates to medical biotechnology experimental provision technical field more particularly to a kind of dyeing apparatus.
Background technique
Immunohistochemistry (Immunohistochemistry, IHC) is to utilize original of the antigen in conjunction with antibody specificity
Reason makes color developing agent (fluorescein, enzyme, the metal ion, isotope) colour developing of labelled antibody determine that tissue is thin by chemically reacting
Antigen (peptide and protein) intracellular positions it, is qualitative and determine quantifier elimination.In medical experiment, using antigen with
The principle that antibody specificity combines carries out immunochemistry dyeing to tissue using different antibodies, is detection body in physiology or disease
One of most common method of changes of function under reason state.
The dyeing course of histotomy is that first histotomy is placed in histotomy holding tank at present, is then being accommodated
First it is added confining liquid in slot, confining liquid is sucked out after one hour for closing, primary antibody dyeing liquor is added, again by one after an anti-dye
Anti-dye liquid is sucked out, and adds PBS rinsing liquid, rinsing liquid is sucked out again after rinsing, and need to generally be rinsed three times, then again
Secondary antibody dyeing liquor is added, secondary antibody dyeing liquor is sucked out again after two anti-dye, PBS rinsing liquid is added, again by rinsing liquid after rinsing
It is sucked out, and generally also needs to be rinsed three times.In the case where sample size is big, the above process is very cumbersome, time-consuming and laborious, and
Histotomy is easily damaged when manual sucking liquid.
Therefore, a kind of dyeing apparatus is needed to solve above-mentioned technical problem.
Utility model content
It is easy to operate the purpose of this utility model is to provide a kind of dyeing apparatus, time and manpower can be saved, simultaneously
Reduce the loss late of histotomy.
For this purpose, the utility model uses following technical scheme:
A kind of dyeing apparatus, including pedestal and cover board, wherein several liquid holding grooves, the lid are offered on the pedestal
The lower end surface of plate is provided with several cylinders, and the open end of the cylinder is covered with counterdie, offers several on the counterdie
Water seepage hole；When the cover plate lid is set on the pedestal, the cylinder and the liquid holding groove are corresponded, and the cylinder is inserted
In the liquid holding groove.
As the optimal technical scheme of dyeing apparatus, the diameter of the water seepage hole is 200 μm -500 μm.
As the optimal technical scheme of dyeing apparatus, the counterdie is made by nylon or fiber.
As the optimal technical scheme of dyeing apparatus, the liquid holding groove and the cylinder are arranged in matrix.
As the optimal technical scheme of dyeing apparatus, the pedestal and the cover board by acrylic or plastic production and
At.
It further include epiphragma as the optimal technical scheme of dyeing apparatus, the epiphragma is covered in the upper surface of the cover board.
As the optimal technical scheme of dyeing apparatus, the epiphragma is by sticky adhesive in the upper surface of the cover board.
As the optimal technical scheme of dyeing apparatus, the epiphragma is made by plastic cement paper.
As the optimal technical scheme of dyeing apparatus, the dyeing apparatus is immunohistochemical staining device.
The utility model compared with the prior art the utility model has the advantages that
1) dyeing apparatus provided by the utility model, when needing replacing liquid, moves in the dyeing course of histotomy
Plate of uncapping can replace the liquid in liquid holding groove, while residual liquid can be arranged by the water seepage hole on counterdie in cylinder
Out, time and manpower is greatly saved in the process for eliminating artificial sucking liquid, and also avoids due to artificial sucking liquid
Caused by injury tissue be sliced the problem of, effectively reduce the loss late of histotomy；
2) dyeing apparatus provided by the utility model is additionally provided with epiphragma, and epiphragma is covered in the upper surface of cover board, for hiding
Light, since the dyeing course duration of histotomy is longer, the setting of epiphragma can effectively prevent liquid in immunostaining mistake
Evaporation in journey guarantees that dyeing course is gone on smoothly.
Detailed description of the invention
Fig. 1 is the decomposition texture schematic diagram of dyeing apparatus provided by the utility model.
In figure:
1- pedestal；2- cover board；3- counterdie；4- epiphragma；
11- liquid holding groove；21- cylinder.
Specific embodiment
The technical solution of the utility model is further illustrated with embodiment with reference to the accompanying drawing.It is understood that this
Locate described specific embodiment and is used only for explaining the utility model, rather than the restriction to the utility model.It further needs exist for
Illustrate, illustrates only part relevant to the utility model for ease of description, in attached drawing and not all.
As shown in Figure 1, the utility model provides a kind of dyeing apparatus, including pedestal 1 and cover board 2, wherein opened on pedestal 1
Equipped with several liquid holding grooves 11, the lower end surface of cover board 2 is provided with several cylinders 21, and the open end of cylinder 21 is covered with counterdie 3,
Several water seepage holes are offered on counterdie 3；When cover board 2 is covered on pedestal 1, cylinder 21 and liquid holding groove 11 are corresponded, and
Cylinder 21 is inserted in liquid holding groove 11.
In the dyeing course of histotomy, cover board 2 is covered on pedestal 1, and histotomy is located in cylinder 21, and liquid is contained
It being placed in liquid holding groove 11, liquid, which enters cylinder 21 by the water seepage hole on counterdie 3, to be dyed or be rinsed to histotomy,
Since cylinder 21 is inserted in liquid holding groove 11, therefore the liquid in liquid holding groove 11 can more successfully penetrate into cylinder 21.When need
When replacing liquid, the liquid in liquid holding groove 11 can be replaced by removing cover board 2, while residual liquid meeting in cylinder 21
By the water seepage hole discharge on counterdie 3, the process of artificial sucking liquid is eliminated, time and manpower is greatly saved, and
The problem of avoiding the slice of the injury tissue as caused by artificial sucking liquid, effectively reduces the loss late of histotomy.
It should be noted that first histotomy is placed by the opening of cylinder 21 when histotomy is put into cylinder 21
In cylinder 21, then counterdie 3 is covered on to the open end of cylinder 21 again；When histotomy takes out out of cylinder 21, first by cylinder
The counterdie 3 of 21 open end is removed, and then again takes out histotomy by the opening of cylinder 21.
Further, on counterdie 3 water seepage hole diameter be 200 μm -500 μm, can guarantee water droplet is formed on counterdie 3 with
In drip process, water drops surface tension not will cause histotomy damage.In the present embodiment, counterdie 3 can be by nylon or fibre
Dimension is made, this is exemplary illustration certainly, and the material of counterdie 3 is not limited thereto, will not enumerate herein.In addition
In the present embodiment, counterdie 3 can be adhered to the open end of cylinder 21 by marine glue, it can also be appreciated that counterdie 3
Fixed form is also not limited to this, also will not enumerate herein.
In the present embodiment, several cylinders 21 of 2 lower end surface of several liquid holding grooves 11 and cover board on pedestal 1 are with square
The arrangement of formation formula, more commonly used is the matrix form of 4*6,6*6 and 9*6.
In the present embodiment, pedestal 1 and cover board 2 are formed by acrylic or plastic production, and acrylic and plastics all have
Reusable and at low cost advantage.
Further, dyeing apparatus provided by the utility model further includes epiphragma 4, and epiphragma 4 is covered in the upper end of cover board 2
Face is used for shading, and since the immunostaining process duration of histotomy is longer, the setting of epiphragma 4 can effectively prevent liquid
Evaporation of body during immunostaining, guarantees that dyeing course is gone on smoothly.
In the present embodiment, epiphragma 4 is by sticky adhesive in the upper surface of cover board 2.Preferably, epiphragma 4 is by plastics gummed paper
It is made.
Further, in this embodiment the dyeing apparatus is preferably immunohistochemical staining device.
Obviously, the above embodiments of the present invention is used for the purpose of clearly illustrating examples for clearly illustrating the present invention, and
It is not limitations of the embodiments of the present invention.For those of ordinary skill in the art, in above description
On the basis of can also make other variations or changes in different ways.There is no need and unable to give all embodiments
Exhaustion.Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention etc., should be included in
Within the protection scope of the utility model claims.
Claims (9)
1. a kind of dyeing apparatus, which is characterized in that including pedestal (1) and cover board (2), wherein the pedestal (1) if on offer
Dry liquid holding groove (11), the lower end surface of the cover board (2) are provided with several cylinders (21), and the open end of the cylinder (21) is covered
It is stamped counterdie (3), offers several water seepage holes on the counterdie (3)；When the cover board (2) is covered on the pedestal (1)
When, the cylinder (21) and the liquid holding groove (11) correspond, and the cylinder (21) is inserted in the liquid holding groove (11).
2. dyeing apparatus according to claim 1, which is characterized in that the diameter of the water seepage hole is 200 μm -500 μm.
3. dyeing apparatus according to claim 1, which is characterized in that the counterdie (3) is made by nylon or fiber.
4. dyeing apparatus according to claim 1, which is characterized in that the liquid holding groove (11) and the cylinder (21) with
Matrix form arrangement.
5. dyeing apparatus according to claim 1, which is characterized in that the pedestal (1) and the cover board (2) are by sub- gram
Power or plastic production form.
6. dyeing apparatus according to claim 1, which is characterized in that further include epiphragma (4), the epiphragma (4) is covered in
The upper surface of the cover board (2).
7. dyeing apparatus according to claim 6, which is characterized in that the epiphragma (4) is by sticky adhesive in the lid
The upper surface of plate (2).
8. dyeing apparatus according to claim 7, which is characterized in that the epiphragma (4) is made by plastic cement paper.
9. dyeing apparatus according to claim 1-8, which is characterized in that the dyeing apparatus is immuning tissue
Learn dyeing apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820397806.2U CN208187823U (en) | 2018-03-22 | 2018-03-22 | A kind of dyeing apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820397806.2U CN208187823U (en) | 2018-03-22 | 2018-03-22 | A kind of dyeing apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208187823U true CN208187823U (en) | 2018-12-04 |
Family
ID=64437214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820397806.2U Active CN208187823U (en) | 2018-03-22 | 2018-03-22 | A kind of dyeing apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208187823U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141543A (en) * | 2020-01-16 | 2020-05-12 | 河南医学高等专科学校 | Pathological microtome for tissue embryology |
| CN112964623A (en) * | 2021-03-23 | 2021-06-15 | 哈尔滨工业大学 | Experimental device for axial permeation of annular tissue engineering scaffold and use method |
-
2018
- 2018-03-22 CN CN201820397806.2U patent/CN208187823U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111141543A (en) * | 2020-01-16 | 2020-05-12 | 河南医学高等专科学校 | Pathological microtome for tissue embryology |
| CN112964623A (en) * | 2021-03-23 | 2021-06-15 | 哈尔滨工业大学 | Experimental device for axial permeation of annular tissue engineering scaffold and use method |
| CN112964623B (en) * | 2021-03-23 | 2023-04-11 | 哈尔滨工业大学 | Experimental device for axial permeation of annular tissue engineering scaffold and use method |
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