CN208579976U - A kind of electron microscopic sample batch negative staining device - Google Patents

Abstract

The utility model discloses a kind of electron microscopic sample batch negative staining devices, including box body, pressing plate and flip lid, box body and flip lid connection, box body are cuboid, are equipped with and the matched strip grooves of platen shape among box body, box body is equipped with several rectangular negative staining slots close to the inboard edge of strip grooves, rectangular negative staining slot is towards a side opening of strip grooves, and box body outer ledge is tag slot, and tag slot is equipped with label corresponding with rectangular negative staining slot.Using negative staining device provided by the utility model, by being equipped with strip grooves in box body, pressing plate is cooperated to use, and several negative staining slots are equipped on the inboard edge close to strip grooves, batch is not only stored into copper mesh, negative staining operates and storage negative staining sample function integration, probability of the reduction because of sample breakage caused by repeatedly shifting copper mesh；And quantitative dropping liquid can be carried out, batch imbibition, batch recycles the operation such as dye liquor and rinsing, can prevent batch negative staining from sample confusion problem easily occurs equipped with mark.

Description

A kind of electron microscopic sample batch negative staining device

Technical field

The utility model relates to electron microscopic sample handling implement field more particularly to a kind of electron microscopic sample batch negative staining devices.

Background technique

Negative staining technology is also known as negative contrast staining, abbreviation negative staining.It is the one kind used first by HaLL and Huxley Staining technique applied to large biological molecule research.Unlike traditional dyeing, when sample carries out negative staining, due to itself and dye It does not react between liquid, therefore sample itself and not colored.It is compared using the great disparity of sample and coloring agent density, in electron dense Black background in can set off by contrast the white pigmented samples of low electron density out, form negative contrast, therefore claim negative staining.The sample of negative staining technology Product are not necessarily to through complex operations such as fixation, dehydration, embedding and ultra-thin sections, but by sample preparation at the suspension with certain density Then liquid is dispersed in on the copper mesh for supporting film, is finally dyed with negative staining liquid to increase contrast, can carry out Electronic Speculum Observation.The technology has easy to operate, quick and convenient, can preferably save sample structure, makes to be contaminated that sample contrast is moderate, differentiates Rate is high, and the advantages that sample and few dye liquor expense, therefore is widely used in bacterium, virus, macromolecular, subcellular fragments, divides From organelle and the samples such as protein crystal analysis.There are many negative staining methods, have support grid drop dye method, single drop cere method, Floating method or negative staining-carbon film method etc. usually select support grid to drip dye method at present, and operation is simple, but are only suitable for quickly preparing single Sample, preparing multiple samples seems that speed is too slow, and needs a twist of the wrist when operation, could not damage support film.It is logical at present Often double faced adhesive tape is sticked on glass slide, is clamped with tweezers with supporting the copper mesh of film placed on it, uses capillary syring toward copper afterwards It nets and titrates sample liquid, sop up surplus liquid with filter paper after absorption 20-40S, then instill negative staining liquid, absorption, drying prepare negative Sample is contaminated, single operation is time-consuming and laborious, but during scientific experiment, and generally there are many different disposals by sample by experimenter Multiple groups comparative experiments, the environment of negative staining, dye liquor usage amount the factors such as size negative staining result can all be had an impact, only unanimously Operation can just obtain reliable and accurate experimental data, it is therefore desirable to sample carry out batch negative staining.And it is needed after drop sample with weight It steams water suitably to rinse, negative staining effect can be effectively improved.Therefore, it is necessary to design it is a kind of can batch processing electron microscopic sample negative staining dress It sets.

Utility model content

In view of this, the present invention provides a kind of electron microscopic sample batch negative staining devices, solve of the existing technology Problem.

The utility model use technological means it is as follows: a kind of electron microscopic sample batch negative staining device, including box body, pressing plate and Flip lid, box body and flip lid connection, the box body are rectangle, be equipped among the box body with the matched strip grooves of platen shape, Box body is equipped with several rectangular negative staining slots, a side opening of the rectangular negative staining slot towards strip grooves, box close to the inboard edge of strip grooves External side edge is tag slot, and tag slot is equipped with label corresponding with rectangular negative staining slot

Preferably, the rectangular negative staining groove depth is the 1/2 of strip grooves, meets dye liquor amount needs while limit.

Preferably, pressing plate is equipped with handle ring, convenient for pulling on or pulling down pressing plate.

Preferably, the long 16cm of box body, high 2cm, wide 6cm, strip flute length 14cm, wide 2cm, deep 1cm, rectangular negative staining flute length 1cm, wide 1cm, deep 0.5cm.The bottom of negative staining slot is matched with the copper mesh that conventional diameter is 1cm, on the other hand its depth conduct The reference standard of dropping liquid amount.

Preferably, rectangular negative staining trench bottom is equipped with several cambered surface raised lines, and cambered surface raised line does not damage copper mesh and can prop up copper Net is tested convenient for picking and placing copper mesh with tweezers side.

Preferably, box body and laminate material are polyester plastics, lightweight and easy to process.

Using a kind of electron microscopic sample batch negative staining device provided by the utility model, by being equipped with strip among box body Slot, cooperation pressing plate uses, and several negative staining slots are equipped on the inboard edge close to strip grooves, and indicates serial number label, not only It for daily storage copper mesh, and can realize batch negative staining and quantitative dropping liquid, be convenient for the batch imbibition of filter paper side, recycle extra dye Liquid prevents pollution environment, can also rinse, and the operation such as dries.In addition copper mesh will be stored, negative staining operates, the copper mesh sample after storage negative staining Product function integration reduces because repeatedly transfer copper mesh be easy to cause sample probability of damage, prevents batch negative staining from easily sending out equipped with mark Raw sample confusion problem,.

Further, the design of the rectangular negative staining slot copper mesh that on the one hand just storage diameter is 1cm, on the other hand convenient for length Filter paper side batch is adsorbed by drying.

Detailed description of the invention

Fig. 1 is a kind of structural schematic diagram of electron microscopic sample batch negative staining device of the utility model；

Fig. 2 is negative the section enlarged diagram of staining bath 5.

Specific embodiment

The principles of the present invention and feature are described below in conjunction with attached drawing, example is served only for explaining that this is practical It is novel, it is not intended to limit the scope of the utility model.

Embodiment one: a kind of electron microscopic sample batch negative staining device, including rectangular box 1, pressing plate 2 and flip lid 3, rectangle Box body 1 and flip lid 3 pass through in side to be articulated and connected, and flip lid matches closure with 1 size of box body；Rectangular box 1 is 16cm long, high 2cm, wide 6cm, be equipped among box body 1 with the matched strip grooves 4 of 2 shape of pressing plate, strip grooves 4 are 14cm long, wide 2cm, deep 1cm, box Body 1 is equipped with negative staining slot 5 close to the inboard edge 6 of strip grooves, and 5 bottom surface of negative staining slot is 1*1cm, deep 0.5cm, and copper mesh diameter is 1cm, just matching is placed in negative staining slot 5, and for negative staining slot 5 towards 4 one side opening of strip grooves, 1 outer ledge of box body is tag slot 7, It is digital equipped with 1..2...14 corresponding with negative staining slot 5 etc., for identifying negative staining slot 5.Pressing plate is equipped with handle ring 8, convenient for behaviour Make, box body 1 and pressing plate 2 are polyester plastics.

One of the present embodiment electron microscopic sample batch negative staining device, is first placed in negative staining slot 5 for copper mesh and saves, to do negative staining Before experiment, flip lid is opened, pressing plate 2 is just matched and is pressed in strip grooves 4, on the one hand the baffle as negative staining slot 5, with negative staining flute profile At the accommodating chamber of 1cm*1cm*0.5cm, it is convenient for covering subject to negative staining slot when dropping liquid, can refer to dropping liquid ensures that dropping liquid amount is consistent, separately On the one hand, strip grooves 4 are flowed into and are recycled to pressing plate 2, extra dye liquor after a certain period of time, is pulled open when negative staining, in addition use strip filter paper Close to 4 inner wall of strip grooves batch absorb dye liquor extra in multiple negative staining slots, after put down pressing plate 2 again, toward negative staining slot 5 instill weight Water rinsing is steamed, pressing plate 2, filter paper imbibition are pulled open, drying can be obtained electron microscopic sample.

Embodiment two: the difference of this implementation two and one is: 5 bottom of negative staining slot is equipped with two cambered surface raised lines 10, by copper mesh 9 It is placed on it, picks and places copper mesh 9 from negative staining slot towards 4 side of strip grooves convenient for tweezers.

In conclusion using a kind of electron microscopic sample batch negative staining device provided by the utility model, by box body Between be equipped with strip grooves, cooperation pressing plate uses, and several negative staining slots are equipped on the inboard edge close to strip grooves, and indicates serial number Label, is applied not only to daily storage copper mesh, and can realize batch negative staining and quantitative dropping liquid, is convenient for the imbibition of filter paper side, recycling Extra dye liquor prevents pollution environment, can also rinse, drying process.In addition copper mesh will be stored, negative staining operates, the copper after storage negative staining Net sample function integration prevents batch negative staining from sample confusion problem easily occurs, and reduces because repeatedly transfer copper mesh be easy to cause sample Product probability of damage.Further, on the one hand rectangular negative staining slot just stores copper mesh, on the other hand convenient for strip filter paper side batch It is adsorbed by drying.

The above is only the preferred embodiment of the utility model only, is not intended to limit the utility model, all at this Within the spirit and principle of utility model, any modification, equivalent substitution, improvement and etc. done should be included in the utility model Within the scope of protection.

Claims (6)

1. a kind of electron microscopic sample batch negative staining device, which is characterized in that including box body, pressing plate and flip lid, box body and flip lid are connected, The box body is rectangle, be equipped among the box body with the matched strip grooves of platen shape, box body is close to the inboard of strip grooves Edge is equipped with several rectangular negative staining slots, and for rectangular negative staining slot towards a side opening of strip grooves, box body outer ledge is tag slot, mark Know area and is equipped with label corresponding with rectangular negative staining slot.

2. a kind of electron microscopic sample batch negative staining device according to claim 1, which is characterized in that the rectangular negative staining groove depth Degree is the 1/2 of strip groove depth.

3. a kind of electron microscopic sample batch negative staining device according to claim 1, which is characterized in that the pressing plate, which is equipped with, to be drawn Bracelet.

4. a kind of electron microscopic sample batch negative staining device according to claim 1, which is characterized in that the long 16cm of box body, High 2cm, wide 6cm, strip flute length 14cm, wide 2cm, deep 1cm, rectangular negative staining flute length 1cm, wide 1cm, deep 0.5cm.

5. a kind of electron microscopic sample batch negative staining device according to claim 1, which is characterized in that the rectangular negative staining slot bottom Portion is equipped with several cambered surface raised lines.

6. a kind of electron microscopic sample batch negative staining device according to claim 1-5, which is characterized in that the box body It is polyester plastics with laminate material.