CN210166293U - Multifunctional analysis device based on blue and white light - Google Patents

Abstract

The utility model discloses a multi-functional analytical equipment based on blue and white light for improve the result observation and the gel operating efficiency of nucleic acid and protein electrophoresis in molecular biology experiment, and the observation and the count of bacterial colony on the solid plate medium in the supplementary conversion experiment. Including quick-witted case upper portion, quick-witted case lower part and the baffle that is equipped with between quick-witted case upper portion and the quick-witted case lower part, through quick-witted case upper portion, the blue light and the white light that quick-witted case lower part was equipped with respectively are as the light source, and relevant accessories such as amber light filter, bacterial colony count pen can realize that nucleic acid and protein electrophoresis's result is observed and the gel operation to can assist the observation and the count of bacterial colony on the solid plate medium, relevant experimentation and experimental result all can be through the image acquisition device record that the top was equipped with, be convenient for exchange and share. Compare single function equipment, this instrument is the integrated device that integrates the required function of a great deal of molecular biology experiment into one body, and the practicality is strong, and the security is high, simple operation, easy to maintain.

Description

Multifunctional analysis device based on blue and white light

Technical Field

The utility model relates to a molecular biology experiment technique not only can be used to improve the result observation and the gel operating efficiency of nucleic acid and protein electrophoresis, still can be used to the observation and the count of bacterial colony on the solid plate medium in the supplementary transformation experiment.

Background

Molecular biology is a fundamental subject that is penetrated from various subjects such as biochemistry, biophysics, genetics, microbiology and the like, takes biomacromolecules (such as proteins, nucleic acids and the like) as research objects, has rapid development, strong frontier and wide application, and is an indispensable important tool for deep research in various fields of life science. Molecular biology itself is a highly practical discipline, and thus many experimental techniques are involved, and nucleic acid electrophoresis, protein electrophoresis and colony counting are important contents.

The nucleic acid electrophoresis, the protein electrophoresis and the colony counting are frequently related experimental techniques in the teaching content of biological experiments, and related experimental teaching items mainly comprise RNA extraction from mouse liver, target gene acquisition by RT-PCR, target gene purification and recovery, preparation of vector pGEX-4T-2, enzyme digestion of target gene/vector, transformant screening, target protein induced expression and PAGE analysis, plasmid DNA extraction and enzyme digestion identification, protein molecular weight determination by SDS-polyacrylamide gel electrophoresis and the like. In practical practice, observation and operation of different electrophoresis results and colony counting need to be realized by different light sources respectively:

1. and (3) observing results of nucleic acid electrophoresis and performing gel cutting operation: to observe the position of the nucleic acid molecules in the agarose gel during electrophoresis, a fluorescent dye is added during the gel preparation. Ethidium Bromide (EB), the most commonly used in laboratories in the past, is a highly sensitive fluorescent dye that intercalates between two bases of the DNA duplex structure and fluoresces under ultraviolet excitation, rendering nucleic acid electrophoresis visible. However, due to the high carcinogenicity of EB, in recent years, GelRed, a novel safe dye, which can be excited by blue light or ultraviolet light, is used as an EB substitute in experiments. In addition, the ultraviolet light commonly used in the past in the experiment can not only damage the nucleic acid sample, but also threaten the human health of an operator. Therefore, the safer blue light excitation is the first choice, on one hand, the blue light has no damage to nucleic acid fragments, and the damage of fragment breakage, crosslinking, replacement and the like caused by irradiation is avoided; on the other hand, the easily exposed parts of the experimenters can be prevented from being damaged by ultraviolet rays, and the defects of using ultraviolet rays can be avoided while high sensitivity is kept.

2. And (3) observing results of protein electrophoresis and performing gel cutting operation: theoretically, after polyacrylamide gel commonly used in protein electrophoresis is stained with Coomassie brilliant blue, protein band results on the gel can be observed under visible light, but experimental results are often unsatisfactory, problems of too deep background, insufficiently clear bands and the like often occur, and difficulty is brought to band distinguishing and measuring. And the white transmission light source is adopted, so that the definition of the bands in the dyeing result and the contrast between the bands and the background can be obviously improved, and the clear observation and operation of the protein electrophoresis result are facilitated.

3. Observation and counting of colonies on solid plate medium: colony counting is one of the most commonly used techniques in microorganism-related experiments, and is commonly used in molecular biology experiments for the evaluation and calculation of competent cell transformation efficiency. Adopt white light source side-emitting illumination, cooperation black background culture dish net and colony counter can assist the observation and the count of colony on the solid plate medium, makes the colony contrast clear visible, easily distinguishes.

Disclosure of Invention

The utility model aims at providing a multi-functional analytical equipment based on blue light and white light source for improve the result observation and the gel operating efficiency of nucleic acid and protein electrophoresis, and can assist the observation and the count of bacterial colony on the solid plate medium in the conversion experiment.

In order to achieve the above purpose, the utility model discloses a technical scheme is:

the utility model provides a multi-functional analytical equipment based on blue and white light, includes quick-witted case upper portion, quick-witted case lower part and the baffle that is equipped with between quick-witted case upper portion and the quick-witted case lower part, wherein: the top surface of the upper part of the case is provided with an image acquisition device, the front surface of the upper part of the case is provided with an observation window, the back surface of the upper part of the case is provided with an upper fan, two sides of the upper part of the case are provided with a left-hand operation port and a right-hand operation port which are symmetrical, wherein the side surface of the upper part of the case where the right-hand operation port is positioned uses the edge of the right side as an axis, the right side is pulled outwards through an arranged handle, the inner side of the front surface of the upper part of the case is provided with a blue light source which is parallel to the observation window, the irradiation direction of the light source is inclined downwards, the opposite side of the; the front of machine case lower part is equipped with blue light switch and white light switch, and the back of machine case lower part is equipped with down fan and power source, and the left and right side inboard of machine case lower part is equipped with parallel left white light lamp area and right white light lamp area, and the bottom of the case inboard is the plane of black area culture dish net, and the space that five sides of machine case lower part enclose is used for accomodating bacterial colony count pen.

Furthermore, the image acquisition device is a camera or a camera, and the lens is positioned in and faces the inside of the case.

Furthermore, the front surface of the upper part of the case is connected with the top surface through an inclined surface, and the observation window is arranged on the inclined surface.

Furthermore, the observation window, the left hand operation port and the right hand operation port are all provided with black covers for avoiding external light interference in the image acquisition process.

Furthermore, the blue light source is provided with a light source cover, and the upper surface of the blue light source is parallel to the top surface of the upper part of the case; the fixed end of the amber color filter is parallel to and equal in height with the light source cover of the blue light source.

Furthermore, the partition board is made of white frosted light-transmitting materials, is placed between the upper portion of the case and the lower portion of the case when needed, and is parallel to the bottom of the case.

The utility model has the advantages that:

the utility model discloses a blue light and white light are as the light source, and relevant accessories such as cooperation light filter, bacterial colony count pen can realize that nucleic acid and protein electrophoresis's result is observed and the gel operation to can assist the observation and the count of bacterial colony on the solid plate medium, relevant experimentation and experimental result all can be through the record of image acquisition device, are convenient for exchange and share. Compare single function equipment, this instrument is the integrated device that integrates the required function of a great deal of molecular biology experiment into one body, and the practicality is strong, and the security is high, simple operation, easy to maintain. For production or scientific research units which often perform nucleic acid, protein electrophoresis and colony counting experiments, the instrument can fully meet the requirements of related experiment operation, and greatly improve the experiment operation efficiency; for experimental teaching, especially for basic experiments with numerous teaching objects, the instrument can relieve the pressure on teaching cost and storage space caused by purchasing multiple single-function devices, save experimental teaching cost and teaching space, and can realize real-time capture of experimental process and results.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an exploded view of the present invention.

1. An image acquisition device; 2. the upper part of the case; 3. an observation window; 4. an upper fan; 5. a right hand operation port; 6. a lower fan; 7. a handle; 8. a partition plate; 9. the lower part of the case; 10. a white light switch; 11. a blue light switch; 12. a power interface; 13. a left hand operation port; 14. a blue light source; 15. an optical filter; 16. a left white light strip; 17. a right white light strip; 18. the bottom of the chassis; 19. colony counting pen.

Detailed Description

The device is integrated equipment, and is divided into an upper part and a lower part from the position of a middle partition plate 8:

the upper part 2 of the case: the top surface is provided with an image acquisition device 1 (a camera or a camera), the lens is positioned and faces the interior of the case, and the operation process and the experimental result in the case can be observed, recorded and shared in real time by connecting a mobile phone, a computer and the like; the front surface and the top surface of the upper part 2 of the case are connected through an inclined plane, the inclined plane is provided with an observation window 3, so that the experimental condition in the case can be observed directly by naked eyes, and the observation window 3 is provided with a black cover for avoiding the interference of external light rays in the image acquisition process; the back of the upper part 2 of the case is provided with an upper fan 4 for heat dissipation; the two sides of the upper part 2 of the case are provided with symmetrical left and right hand operation ports 13 and 5 which are used for an operator to operate gel or a solid culture medium flat plate in the case, the two operation ports are provided with black covers and are also used for avoiding external light interference in the image acquisition process, wherein the side surface of the upper part 2 of the case where the right hand operation port 5 is positioned can take the edge of the right side thereof as an axis, the right hand operation port is pulled outwards rightwards through a handle 7, a blue light source 14 (a blue LED with the wavelength of 470nm and the total power of more than 40W) which is parallel to the observation window 3 is arranged on the inner side of the front surface of the upper part 2 of the case after pulling, the light source irradiation direction is inclined downwards, and the upper surface; an amber optical filter 15(580nm) is arranged opposite to the blue light source 14, one side of the optical filter 15 is fixed on the inner side of the back of the upper part 2 of the case, the fixed end of the optical filter 15 is parallel to and equal in height with a light source cover of the blue light source 14, when the optical filter 15 is used, the free end opposite to the fixed end needs to be placed on the light source cover of the blue light source 14 to be parallel to the top surface of the upper part 2 of the case, when the optical filter 15 is not needed, the optical filter is closely attached to the inner side of the back of the upper part 2 of the case to be contained, and a crease perpendicular to the.

The lower part 9 of the case: the front surfaces are respectively provided with a blue light switch 11 and a white light switch 10; the back surface is provided with a lower fan 6 and a power interface 12 for heat dissipation; the left and right sides inboard is installed parallel left white light lamp area 16 and right white light lamp area 17 respectively (full wavelength white LED, more than the total power 16W), and 18 inboard sides at the bottom of the machine are the plane of black area culture dish net, and the space that five planes enclose can be used for accomodating bacterial colony count pen 19.

The partition plate 8: the partition plate 8 is made of white frosted light-transmitting material, and can be placed between the upper part and the lower part in the case by pulling the right side door of the upper part 2 of the case open when needed and is parallel to the case bottom 18; can be taken out when not needed.

The utility model discloses observation window 3 and left hand operation mouth 13, right hand operation mouth 5 all are furnished with the black lid for avoid external light to disturb in the image acquisition process.

The utility model discloses application method as follows:

1. and (3) observing results of nucleic acid electrophoresis and performing gel cutting operation: the blue light switch 11 is opened, the right sliding door of the upper part 2 of the case is pulled open, the optical filter 15 is lifted, the optical filter 15 can avoid discomfort of blue light to eyes of an operator during observation, agarose gel for nucleic acid electrophoresis is placed on the partition plate 8, the partition plate 8 is placed in the case, the blue light source 14 is an epi-illumination source relative to the gel at the moment, the right sliding door is closed, the gel is observed from the observation window 3 (black cover is removed), the gel position can be adjusted through the left hand operation port 13 and the right hand operation port 5 (black cover is removed), result observation and gel cutting are realized, when an operation process or an experiment result needs to be recorded, the lens of the image acquisition device 1 can be adjusted through the left hand operation port 13 and the right hand operation port 5, and the black covers of the left hand operation port 13 and the right hand operation port 5 and the observation.

2. And (3) observing results of protein electrophoresis and performing gel cutting operation: the white light switch 10 is opened, the right sliding door of the upper part 2 of the case is pulled open, the optical filter 15 is stored, polyacrylamide gel for protein electrophoresis is placed on the partition plate 8, the partition plate 8 is placed in the case, the left white light strip 16 and the right white light strip 17 are transmission light sources relative to the gel, the right sliding door is closed, the gel is observed from the observation window 3 (the black cover is removed), the gel position can be adjusted through the left hand operation port 13 and the right hand operation port 5 (the black cover is removed), result observation and gel cutting are achieved, when an operation process or an experiment result needs to be recorded, the lens of the image acquisition device 1 can be adjusted through the left hand operation port 13 and the right hand operation port 5, and then the left hand operation port 13 and the right hand operation port 5 and the black cover of the observation window 3.

3. Auxiliary solid plate culture medium colony observation and counting: open white light switch 10, pull open 2 right side sliding doors on quick-witted case upper portion, accomodate light filter 15, take out baffle 8, take out bacterial colony count pen 19, the solid flat board that will grow the bacterial colony is arranged in bottom of the case 18, for the flat board this moment, left and right white light lamp area 16, 17 are the side illumination light source, close the right side sliding door, observe the bacterial colony on the flat board from observation window 3 (getting rid of the black lid), through left and right hands operation mouth 13, 5 (getting rid of the black lid) can adjust the flat board position, realize the bacterial colony and observe, cooperation bacterial colony count pen 19 can realize the bacterial colony count, when needing to record operation process or experimental result, can be earlier through left and right hands operation mouth 13, 5 adjust the camera lens of image acquisition device 1, the black lid that covers left and right hands operation mouth 13 again respectively, 5 and observation window.

Claims (6)

1. A multifunctional analysis device based on blue and white light is characterized in that: including baffle (8) that are equipped with between quick-witted case upper portion (2), quick-witted case lower part (9) and quick-witted case upper portion (2) and quick-witted case lower part (9), wherein:

the top surface of the upper part (2) of the case is provided with an image acquisition device (1), the front surface of the upper part (2) of the case is provided with an observation window (3), the back surface of the upper part (2) of the case is provided with an upper fan (4), two sides of the upper part (2) of the case are provided with a left hand operation port (13) and a right hand operation port (5) which are symmetrical, wherein the right hand operation port (5) is positioned on the side surface of the upper part (2) of the case and the right side edge is taken as an axis, the handle (7) is pulled out rightwards, the blue light source (14) which is parallel to the observation window (3) is arranged on the inner side of the front surface of the upper part (2) of the case, the light source irradiation direction is inclined downwards, the filter (15) is arranged opposite to the blue light source (14), the single side of the optical filter (15) is fixed on the inner side of the back of the upper part (2) of the case, and a crease which is vertical to the side of the upper part (2) of the case is arranged on the optical filter (15) close to the fixed end, so that the optical filter (15) is convenient to lift and store;

the front of the lower part (9) of the case is provided with a blue light switch (11) and a white light switch (10); the back is provided with a lower fan (6) and a power interface (12); the inboard left white light lamp area (16) and right white light lamp area (17) that are equipped with the parallel of the right side of a left side, the inboard plane that is the black area culture dish net of bottom of the case (18), the space that five surfaces enclose is used for accomodating bacterial colony count pen (19).

2. The multifunctional blue-white light based analyzer according to claim 1, wherein: the image acquisition device (1) is a camera or a camera, and the lens is positioned and faces the inside of the case.

3. The multifunctional blue-white light based analyzer according to claim 1, wherein: the front surface and the top surface of the upper part (2) of the case are connected through an inclined surface, and the observation window (3) is arranged on the inclined surface.

4. The multifunctional blue-white light based analyzer according to claim 1, wherein: the observation window (3), the left hand operation port (13) and the right hand operation port (5) are all provided with black covers for avoiding the interference of external light rays in the image acquisition process.

5. The multifunctional blue-white light based analyzer according to claim 1, wherein: the blue light source (14) is provided with a light source cover, and the upper surface of the blue light source is parallel to the top surface of the upper part (2) of the case; the fixed end of the amber color filter (15) is parallel to and equal in height with the light source cover of the blue light source (14).

6. The multifunctional blue-white light based analyzer according to claim 1, wherein: the partition plate (8) is made of white frosted light-transmitting material, is placed between the upper part (2) and the lower part (9) of the case when needed, and is parallel to the bottom (18) of the case.

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