CN218893658U

CN218893658U - Plastic dyeing cylinder for fluorescence in-situ hybridization experiment

Info

Publication number: CN218893658U
Application number: CN202223445797.5U
Authority: CN
Inventors: 余创波; 涂传明
Original assignee: Guangzhou Yixin Biotechnology Co ltd
Current assignee: Guangzhou Yixin Biotechnology Co ltd
Priority date: 2022-12-22
Filing date: 2022-12-22
Publication date: 2023-04-21
Anticipated expiration: 2032-12-22

Abstract

The utility model discloses a plastic dyeing cylinder for a fluorescence in situ hybridization experiment, which belongs to the technical field of fluorescence in situ hybridization experiments and comprises a cylinder body, wherein the cylinder body is made of black plastic, an observation port is formed in the position, close to the right, of the front side of the cylinder body, a baffle is fixedly arranged in the observation port, the baffle is made of transparent plastic, height scale marks are carved on the position, located on one side of the observation port, of the outer surface of the cylinder body, a slide socket is formed in the position, located on the left side, of the upper side of the cylinder body, a liquid injection port is formed in the position, located on the right side, of the upper side of the cylinder body, a cover plate is movably arranged on the liquid injection port, a hinge is jointly arranged between the left side of the cover plate and the upper side of the cylinder body, and two magnets with opposite polarities are arranged between the right side of the cover plate and the liquid injection port. Therefore, the light-shielding condition of the fluorescence in-situ hybridization experiment can be optimized, so that the influence of the environment on the fluorescence quenching of the probe is weakened.

Description

Plastic dyeing cylinder for fluorescence in-situ hybridization experiment

Technical Field

The utility model relates to a plastic dyeing cylinder, in particular to a plastic dyeing cylinder for a fluorescent in-situ hybridization experiment, and belongs to the technical field of fluorescent in-situ hybridization experiments.

Background

Fluorescent In Situ Hybridization (FISH) is a technique for determining the morphology and distribution of stained cells or organelles after hybridization with a specific probe or the localization of DNA regions or RNA molecules combined with the fluorescent probe in chromosomes or other organelles by utilizing the hybridization of the fluorescent labeled specific nucleic acid probe with corresponding target DNA molecules or RNA molecules in cells and observing fluorescent signals under a fluorescent microscope or a confocal laser scanner, thereby detecting chromosome and gene abnormalities in cell and tissue samples. The FISH basic experimental flow comprises sample preparation, probe labeling, hybridization, (chromosome banding), fluorescence microscopy detection and result analysis, and can be clinically applied to the identification of diseases such as breast cancer, cervical cancer, non-small cell lung cancer, prostate cancer, bladder cancer, leukemia and the like, wherein the breast cancer (Her 2) identification method is the most typical.

In the fluorescent in situ hybridization experiment process, multiple washing and dehydration steps are needed, a staining jar is needed for experiment, and after slide hybridization, subsequent washing of the slide needs to be performed in a dark place. The currently used 5-piece glass dyeing vat is strong in light transmittance, so that the influence of the environment on fluorescent quenching of the probe is large, and when a small amount of samples are prepared, reagent waste is easily caused if 20 pieces of black plastic dyeing vat are used, so that the plastic dyeing vat for fluorescent in-situ hybridization experiments is provided.

Disclosure of Invention

The utility model aims to solve the technical problem of providing a plastic dyeing cylinder for a fluorescence in-situ hybridization experiment, which can optimize the light-shielding condition of the fluorescence in-situ hybridization experiment, so as to weaken the influence of the environment on the fluorescent quenching of a probe and solve the technical problem of poor light-shielding property of a five-piece dyeing cylinder in the prior art.

In order to solve the problems, the following technical scheme is provided:

the utility model provides a be used for fluorescence normal position hybridization experiment plastics staining jar, includes the cylinder body, the cylinder body is black plastics material, the front side of cylinder body is close to the position on the right and has seted up the viewing aperture, and the inside fixed mounting of viewing aperture has the baffle, the baffle is transparent plastics material, and the surface of cylinder body is located the position of viewing aperture one side and carves the mark of having high scale, slide socket has been seted up to the position on the left side of the upside of cylinder body, annotate the liquid mouth has been seted up to the position on the right side of cylinder body, movable mounting has the apron on annotating the liquid mouth, the last side fixed mounting of apron has the handle, install the hinge jointly between the left side of apron and the upside of cylinder body, be provided with two magnets that the polarity is opposite between the right side of apron and the annotating the liquid mouth, the apron passes through hinge movable mounting in annotating the liquid mouth, the position that the upside of cylinder body is located slide socket top is provided with seal assembly.

Further, the sealing assembly comprises a pressing plate, sealing gaskets are fixedly arranged on the lower side face of the pressing plate, handles are fixedly arranged in the middle of the upper side face of the pressing plate, the number of the sealing gaskets is five and corresponds to that of the slide sockets, and the sealing gaskets are made of rubber materials.

Furthermore, the number of the slide insertion holes is five, the slide insertion holes are distributed on the upper side face of the cylinder body in parallel at equal intervals, and the distance between two adjacent slide insertion holes is 2 mm.

Further, the surface movable mounting of cylinder body has the pull rod, the equal fixedly connected with connecting axle in both ends of pull rod, the pull rod is U type structure and through connecting axle movable mounting on the cylinder body.

Compared with the prior art, the utility model has the beneficial effects that:

in this application, utilize box-like structure's cylinder body can reduce the consumption of reagent, this staining jar only needs about 30mL corresponding reagent just enough be used for 5 slide experiments, and the staining jar of this application adopts black plastics material, makes the staining jar possess the light-resistant function, can optimize fluorescence in situ hybridization experiment light-resistant condition to weaken the influence of environment to probe fluorescence quenching.

Specific embodiments of the utility model are disclosed in detail below with reference to the following description and drawings, indicating the manner in which the principles of the utility model may be employed. It should be understood that the embodiments of the utility model are not limited in scope thereby. The embodiments of the utility model include many variations, modifications and equivalents within the spirit and scope of the appended claims.

Drawings

The accompanying drawings are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate the utility model and together with the embodiments of the utility model, serve to explain the utility model. In the drawings:

FIG. 1 is a schematic diagram of a preferred embodiment of a plastic staining jar for fluorescence in situ hybridization experiments according to the present utility model;

FIG. 2 is a schematic diagram of a preferred embodiment of a plastic staining jar for fluorescence in situ hybridization experiments according to the present utility model;

FIG. 3 is a schematic diagram of a preferred embodiment of a plastic staining jar for fluorescence in situ hybridization experiments according to the present utility model.

In the figure: 1. a cylinder; 2. a pull rod; 3. a connecting shaft; 4. an observation port; 5. a baffle; 6. slide sockets; 7. a liquid injection port; 8. a cover plate; 9. a handle; 10. a hinge; 11. a seal assembly; 111. a pressing plate; 112. a sealing gasket; 113. a handle.

Detailed Description

The utility model is further described in connection with the following detailed description, in order to make the technical means, the creation characteristics, the achievement of the purpose and the effect of the utility model easy to understand.

As shown in fig. 1-3, the plastic dyeing cylinder for fluorescent in situ hybridization experiments provided in this embodiment includes a cylinder body 1, the cylinder body 1 is made of black plastic, the cylinder body 1 made of black plastic has excellent light-shielding property, the light-shielding condition for fluorescent in situ hybridization experiments can be optimized, thereby reducing the influence of environment on fluorescent quenching of probes, an observation port 4 is provided at the position of the front side of the cylinder body 1 near the right, a baffle 5 is fixedly installed in the observation port 4, the baffle 5 is made of transparent plastic, a height scale mark is engraved at the position of the outer surface of the cylinder body 1 at one side of the observation port 4, the content of liquid in the cylinder body 1 can be observed through the baffle 5 made of transparent material, thereby facilitating the addition of reagents in the cylinder body 1, a slide socket 6 is provided at the position of the left side of the upper side of the cylinder body 1, the slide socket 6 has a size of 1.2 mm 28 mm, the slide is inserted into the cylinder body 1 through the slide insertion opening 6, the height (55 mm) of the cylinder body 1 is slightly lower than the length (75 mm) of a common slide, the position on the right side of the upper side surface of the cylinder body 1 is provided with a liquid injection opening 7, the liquid injection opening 7 is used for injecting and pouring reagents in the cylinder body 1, the size of the liquid injection opening 7 is 15 mm by 28 mm, the liquid injection opening 7 is movably provided with a cover plate 8, the upper side surface of the cover plate 8 is fixedly provided with a lifting handle 9, the left side of the cover plate 8 and the upper side surface of the cylinder body 1 are jointly provided with a hinge 10, two magnets with opposite polarities are arranged between the right side surface of the cover plate 8 and the liquid injection opening 7, the magnets are used for fixing when the cover plate 8 covers, the cover plate 8 is movably arranged in the liquid injection opening 7 through the hinge 10, the position of the upper side surface of the cylinder body 1 above the slide insertion opening 6 is provided with a sealing component 11, the sealing component 11 is used for shielding the slide insertion opening 6, dust is prevented from falling into the cylinder 1 through the slide socket 6 when not in use.

Preferably, the sealing assembly 11 comprises a pressing plate 111, a sealing pad 112 is fixedly arranged on the lower side surface of the pressing plate 111, a handle 113 is fixedly arranged in the middle of the upper side surface of the pressing plate 111, the number of the sealing pads 112 is five and corresponds to that of the slide sockets 6, the sealing pads 112 are made of rubber, and the sealing pads 112 made of rubber can block the slide sockets 6, so that ash falling through the slide sockets 6 is avoided.

Preferably, the number of the slide sockets 6 is five, the slide sockets 6 are distributed on the upper side surface of the cylinder body 1 at equal intervals in parallel, the interval between two adjacent slide sockets 6 is 2 mm, and the five slide sockets 6 can be used for experiments of a small amount of samples.

Preferably, the surface movable mounting of cylinder body 1 has pull rod 2, and the equal fixedly connected with connecting axle 3 in both ends of pull rod 2, and pull rod 2 is U type structure and through connecting axle 3 movable mounting on cylinder body 1, and pull rod 2 is used for carrying of cylinder body 1 to remove.

The application principle and the application flow of the utility model are as follows: during the use, carry the cylinder body 1 through pull rod 2 and remove experimental place, open the apron 8 on the notes liquid mouth 7, pour the reagent into the cylinder body through notes liquid mouth 7 and cover apron 8 after, in the reagent pouring process, can give the content of observing the interior liquid of cylinder body 1 through transparent material's baffle 5, thereby make things convenient for the interpolation of reagent in the cylinder body 1, utilize handle 113 to say seal assembly 11 and take off, then say that the slide of experiment inserts cylinder body 1 through slide socket 6, cylinder body 1 is black plastics material, the cylinder body 1 of black plastics material has excellent light-proof nature, can optimize fluorescence in situ hybridization experiment light-proof condition, thereby weaken the influence of environment to the probe fluorescence quenching.

In the description of the present utility model, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present utility model and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific direction, be configured and operated in the specific direction, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present utility model, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "provided," "connected," and the like are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to specific circumstances.

While the utility model has been described in connection with specific embodiments, it will be apparent to those skilled in the art that the description is intended to be illustrative and not limiting in scope. Various modifications and alterations of this utility model will occur to those skilled in the art in light of the spirit and principles of this utility model, and such modifications and alterations are also within the scope of this utility model.

Claims

1. A be used for fluorescence normal position hybridization experiment plastics staining jar, its characterized in that, including cylinder body (1), viewing aperture (4) have been seted up to the front side of cylinder body (1) near the position on the right, and the inside fixed mounting of viewing aperture (4) has baffle (5), slide socket (6) have been seted up to the left position of the upside of cylinder body (1), annotate liquid mouth (7) have been seted up to the position on the upside right of cylinder body (1), movable mounting has apron (8) on annotating liquid mouth (7), the upside fixed mounting of apron (8) has handle (9), install hinge (10) jointly between the left side of apron (8) and the upside of cylinder body (1), the position that the upside of cylinder body (1) is located slide socket (6) top is provided with seal assembly (11).

2. A plastic staining jar for fluorescent in situ hybridization experiments as claimed in claim 1 wherein the sealing assembly (11) comprises a pressing plate (111), a sealing pad (112) is fixedly arranged on the lower side surface of the pressing plate (111), and a handle (113) is fixedly arranged in the middle of the upper side surface of the pressing plate (111).

3. The plastic staining jar for fluorescent in situ hybridization experiments as claimed in claim 1, wherein the number of the slide sockets (6) is five, the slide sockets are distributed in parallel and equidistant on the upper side surface of the cylinder body (1), and the distance between two adjacent slide sockets (6) is 2 mm.

4. The plastic staining jar for the fluorescence in situ hybridization experiment according to claim 1, wherein the jar body (1) is made of black plastic material, the baffle plate (5) is made of transparent plastic material, and the position of the outer surface of the jar body (1) on one side of the observation port (4) is carved with height scale marks.

5. The plastic dyeing cylinder for the fluorescence in situ hybridization experiment according to claim 1, wherein a pull rod (2) is movably mounted on the outer surface of the cylinder body (1), connecting shafts (3) are fixedly connected to two ends of the pull rod (2), and the pull rod (2) is of a U-shaped structure and is movably mounted on the cylinder body (1) through the connecting shafts (3).

6. A plastic staining jar for fluorescent in situ hybridization experiments as claimed in claim 2 wherein the number of the sealing gaskets (112) is five and corresponds to the slide socket (6), and the sealing gaskets (112) are made of rubber.

7. The plastic staining jar for the fluorescence in situ hybridization experiment according to claim 1, wherein two magnets with opposite polarities are arranged between the right side surface of the cover plate (8) and the liquid injection port (7), and the cover plate (8) is movably installed in the liquid injection port (7) through a hinge (10).