CN105938065B - The fast group pen of service speed is applied in the detection of cell climbing sheet immunohistochemistry - Google Patents
The fast group pen of service speed is applied in the detection of cell climbing sheet immunohistochemistry Download PDFInfo
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- CN105938065B CN105938065B CN201610404345.2A CN201610404345A CN105938065B CN 105938065 B CN105938065 B CN 105938065B CN 201610404345 A CN201610404345 A CN 201610404345A CN 105938065 B CN105938065 B CN 105938065B
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- 230000009194 climbing Effects 0.000 title claims abstract description 24
- 238000003364 immunohistochemistry Methods 0.000 title claims abstract description 19
- 238000001514 detection method Methods 0.000 title claims abstract description 6
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- 238000004043 dyeing Methods 0.000 claims abstract description 14
- 230000001744 histochemical effect Effects 0.000 claims abstract description 7
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- 238000004113 cell culture Methods 0.000 claims abstract description 5
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- 230000000694 effects Effects 0.000 claims description 8
- 239000012188 paraffin wax Substances 0.000 claims description 8
- 238000013115 immunohistochemical detection Methods 0.000 claims description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 claims description 5
- WZUVPPKBWHMQCE-XJKSGUPXSA-N (+)-haematoxylin Chemical compound C12=CC(O)=C(O)C=C2C[C@]2(O)[C@H]1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-XJKSGUPXSA-N 0.000 claims description 4
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Natural products C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 claims description 4
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- 108090000790 Enzymes Proteins 0.000 claims description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 3
- 229930040373 Paraformaldehyde Natural products 0.000 claims description 3
- 235000021355 Stearic acid Nutrition 0.000 claims description 3
- 229920004890 Triton X-100 Polymers 0.000 claims description 3
- 239000013504 Triton X-100 Substances 0.000 claims description 3
- 239000012122 aqueous mounting media Substances 0.000 claims description 3
- 238000004821 distillation Methods 0.000 claims description 3
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- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 3
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 3
- 229920002866 paraformaldehyde Polymers 0.000 claims description 3
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- 238000005406 washing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012224 working solution Substances 0.000 claims description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 claims 3
- 239000011435 rock Substances 0.000 claims 1
- 238000011532 immunohistochemical staining Methods 0.000 abstract description 7
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 12
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 8
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 4
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 4
- 235000013871 bee wax Nutrition 0.000 description 4
- 239000012166 beeswax Substances 0.000 description 4
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 4
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 4
- 239000004793 Polystyrene Substances 0.000 description 3
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- 239000001293 FEMA 3089 Substances 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- RWGFKTVRMDUZSP-UHFFFAOYSA-N cumene Chemical group CC(C)C1=CC=CC=C1 RWGFKTVRMDUZSP-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 238000003365 immunocytochemistry Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention discloses a kind of group pens that service speed is fast to apply in the detection of cell climbing sheet immunohistochemistry, and cell inoculation is carried out cell climbing sheet in glass slide formula culture dish, immunocyte histochemical stain identification is carried out after cell culture;The glass slide formula culture dish is equipped with culture dish body and ware lid, and culture dish figure number is divided to two kinds of single hole room and porous room;Each pore chamber area is greater than glass slide, and ware body bottom is used directly as glass slide, and ware body bottom lateral surface is equipped with rectangle frame trace line chase corresponding with glass slide size, under external force can deviate from the bottom of ware body along rectangle frame trace line chase.The various immunohistochemical stainings that the present invention is suitable for glass slice are tested, and antibody and reagent dosage can be substantially reduced, and are avoided liquid trickling when dyeing and are spread, improve service speed.It is especially applicable for carrying out extensive, the other immunohistochemical staining of large sample multiple groups in the experimental study of cell climbing sheet or cell smear.
Description
The application is application number: 201410500701.1, the applying date: 2014.9.26, title: " multipurpose immunohistochemistry pen
The divisional application of application in cell climbing sheet Immunohistochemical detection ".
Technical field
The present invention relates to a kind of application of immunohistochemistry pen in cell climbing sheet Immunohistochemical detection.
Background technique
With specific antibody to the labels of certain chemical constituents analysis in histotomy and its cell climbing sheet and its content into
Row tissue and cell in-situ are qualitative, position or quantitative study, this technology are known as immunocytochemistry
(immunocytochemistry) technology.
Usual cell climbing sheet Immunohistochemical detection is to carry out dying operation one by one to individual cell climbing sheet, generally
A kind of antibody, the detection of albumen and gene can only be done to single sample, it is difficult to which competent extensive, multisample and the other medicine of multiple groups are real
Test scientific research.
Summary of the invention
It is suitable for various immunohistochemical stainings the purpose of the present invention is to provide one kind to test, antibody can be substantially reduced
And reagent dosage, it avoids liquid trickling when dyeing and spreads, improve the multipurpose immunohistochemistry pen of service speed in cell climbing sheet
Application in Immunohistochemical detection.
The technical solution of the invention is as follows:
The application of a kind of multipurpose immunohistochemistry pen in cell climbing sheet Immunohistochemical detection, it is characterized in that: by thin
Born of the same parents are inoculated in glass slide formula culture dish and carry out cell climbing sheet, and immunocyte histochemical stain mirror is carried out after cell culture
It is fixed;The glass slide formula culture dish is equipped with culture dish body and ware lid, and culture dish figure number is divided to two kinds of single hole room and porous room;Each hole
Room area is greater than glass slide, and ware body bottom is used directly as glass slide, and ware body bottom lateral surface is equipped with corresponding with glass slide size
Rectangle frame trace line chase under external force can deviate from the bottom of ware body along rectangle frame trace line chase;
The immunocyte histochemical stain identification successively includes the following steps:
(1) 15 min are fixed with ice acetone or 4% paraformaldehyde is fixed;
(2) flowing water rinses, and is cleaned sample 3 times with PBS;
(3) 10 min are incubated for 0.5%Triton X-100;
(4) 0.3%H2O2It is incubated for 10 min;
(5) after cleaning sample 3 times with PBS, hot wind is sufficiently dry;
(7) ink is drawn with immunohistochemistry pen on cell climbing sheet and divides 2-20 separation dyeing area;
(8) air is sufficiently dry;
(9) it is closed with normal two antiserum and is incubated for 10 min;
(10) mouse is added dropwise respectively in each cell dyeing area or rabbit-anti first antibody is incubated for 30~60 min;
(11) it absorbs each cell dyeing area liquid and is cleaned sample 3 times with PBS;
(12) enzyme-linked mouse is added dropwise or rabbit secondary antibody working solution is incubated for 30~60 min;
(13) PBS is cleaned sample 3 times;
(14) DAB develops the color, and is protected from light, under the microscope;
(15) distillation washing;
(16) haematoxylin lining dye;
(17) hydrochloride alcohol breaks up, and originally washes;
(18) aqueous mounting medium mounting.
The ink is prepared from the following ingredients in percentage by mixing and forms: rosin 2 ~ 6%, beeswax 18 ~ 26%, paraffin 3 ~ 8%,
Isopropyl ether 5 ~ 10%, methylene chloride 5 ~ 10%, gasoline 25 ~ 30%, carbon disulfide 12 ~ 17%, carbon tetrachloride 2 ~ 6%, chloroform 2 ~ 6%, ring
Ketone 2 ~ 6%;The sum of above-mentioned dosage of each component is 100%;
Or the ink is made of following component mixing: polystyrene 2 ~ 4%, DMF9 ~ 11%, THF9 ~ 11%, rosin 2 ~ 4%,
Beeswax 13 ~ 16%, paraffin 2 ~ 4%, isopropyl ether 4 ~ 6%, methylene chloride 4 ~ 6%, gasoline 18 ~ 22%, carbon disulfide 9 ~ 11%, carbon tetrachloride
9 ~ 11%, chloroform 1 ~ 2%, cyclohexanone 1 ~ 2%, kerosene 2 ~ 4%, turpentine oil 1 ~ 2%;The sum of above-mentioned dosage of each component is 100%;
Or the ink is made of following component mixing: stearic acid 30 ~ 36%, 15 ~ 20%, 46 DEG C of paraffin 9 ~ 15% of benzene, isopropyl
Ether 7 ~ 11%, carbon disulfide 15 ~ 20%, chloroform 15 ~ 20%;The sum of above-mentioned dosage of each component is 100%.
It under external force can be by the bottom of ware body before the abjection of rectangle frame trace line chase, first with recessed along sharp keen cutter
Ditch is drawn, is hooked, and then adds to be deviate from the single hole slot bottom of ware body with external force in ware bottom surface.
The immunohistochemistry pen includes pen container, the spongioid cylindrical body pen core of setting in pen container, is perfused with ink in pen container,
Wooden pen core is arranged in spongioid cylindrical body pen core front end;Spongioid cylindrical body pen core is placed in pen container, and pen container is rectangular
Body or cylindrical body, it is identical that pen container mouth outside has screw thread to match with the internal screw thread of interior pen cap;Stainless shot one is placed in pen container, is shaken
Make the effect for mixing ink when shaking pen container;The interior pen cap rise closing pen container mouth, prevent ink volatilization keep its liquid phase state and
Couple the effect of outer pen cap, inner surface has the internal screw thread with the conjunction of pen container tone, can spiral cover pen container mouth, the outer surface of interior pen cap is set
There is the fore shaft for matching and coincideing with outer pen cap inner lip recessed, cylindrical body pen core end side is equipped with ink brush;The inner lip of outer pen cap
It is recessed to be equipped with fore shaft week, matches and coincide with interior pen cap outer lip, pen cap and fixed interior pen cap are integrated unlatching pen container mouth in closing,
And play a part of to propose ink brush.
One angle of porous room culture dish is in bevel-faced form, facilitates bearing mark;An angle for cultivating ware lid is in and culture
The bevel-faced form that ware cooperates in the angle on inclined-plane;Cultivate interleave depth >=10mm of ware lid and culture dish previous anastomotic periphery.
The present invention is conducive to the dyeing of immunohistochemistry, and staining procedure is similar to Immunohistochemistry, but sample process
Temperature, the time, the standards such as reagent concentration are consistent, and the primary antibody type of label is more, and comparativity and reliability are significantly increased!Processing
Sample size it is more, efficient quick.PBS cleaning, serum are incubated for, mark secondary antibody, and lining dye (haematoxylin) etc. does not need the reality separated
It tests operation and can synchronize and handled, convenient and efficient, experiment condition standard is consistent and easy to control.It is carried out using large area cell climbing sheet
The temperature of its processing of cell culture, the time, the standards such as reagent concentration are consistent, keep the comparativity of experimental result and reliability aobvious
It writes and improves.The quantity of Tissue Culture Dish hole slot is reduced, easy to operate, efficient quick.Specific ink formulations have fully ensured that work
Make effect.It is tested suitable for various immunohistochemical stainings, including;Paraffin tissue sections, frozen tissue section and cell are climbed
The immunohistochemical staining of piece is tested, and antibody and reagent dosage can be substantially reduced, and is avoided liquid trickling when dyeing and is spread, mentions
High service speed;It is especially applicable for carrying out on a large scale in the experimental study of cell climbing sheet or cell smear, large sample multiple groups are other
Immunohistochemical staining.
The group pen is suitable for carrying out on the cell climbing sheet of the histotomy that glass is carrier and polystyrene material carrier
Various immunohistochemical staining experiments, can substantially reduce antibody and reagent dosage, avoid liquid trickling when dyeing and spread, mention
High service speed.It can carry out extensive, multisample and the other medical experiment scientific research of multiple groups.
Detailed description of the invention
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is the structural schematic diagram of culture dish of the present invention.
Fig. 2 is the structural schematic diagram of immunohistochemistry pen.
Fig. 3 is ware body bottom abjection schematic diagram.
Specific embodiment
The application of a kind of multipurpose immunohistochemistry pen in cell climbing sheet Immunohistochemical detection, by cell inoculation in load
Cell climbing sheet is carried out in slide formula culture dish, immunocyte histochemical stain identification is carried out after cell culture;The load
Slide formula culture dish is equipped with culture dish body 1 and ware lid 2, and culture dish figure number is divided to two kinds of single hole room and porous room;Each pore chamber area
Greater than glass slide, ware body bottom 3 is used directly as glass slide, and ware body bottom lateral surface is equipped with rectangle corresponding with glass slide size
Frame trace line chase 4 under external force can deviate from the bottom of ware body along rectangle frame trace line chase;
The immunocyte histochemical stain identification successively includes the following steps:
(1) 15 min are fixed with ice acetone or 4% paraformaldehyde is fixed;
(2) flowing water rinses, and is cleaned sample 3 times with PBS;
(3) 10 min are incubated for 0.5%Triton X-100;
(4) 0.3%H2O2It is incubated for 10 min;
(5) after cleaning sample 3 times with PBS, hot wind is sufficiently dry;
(7) ink is drawn with immunohistochemistry pen on cell climbing sheet and divides 2-20 separation dyeing area;
(8) air is sufficiently dry;
(9) it is closed with normal two antiserum and is incubated for 10 min;
(10) mouse is added dropwise respectively in each cell dyeing area or rabbit-anti first antibody is incubated for 30~60 min;
(11) it absorbs each cell dyeing area liquid and is cleaned sample 3 times with PBS;
(12) enzyme-linked mouse is added dropwise or rabbit secondary antibody working solution is incubated for 30~60 min;
(13) PBS is cleaned sample 3 times;
(14) DAB develops the color, and is protected from light, under the microscope;
(15) distillation washing;
(16) haematoxylin lining dye;
(17) hydrochloride alcohol breaks up, and originally washes;
(18) aqueous mounting medium mounting.
The ink is prepared from the following ingredients in percentage by mixing and forms: rosin 2 ~ 6%, beeswax 18 ~ 26%, paraffin 3 ~ 8%,
Isopropyl ether 5 ~ 10%, methylene chloride 5 ~ 10%, gasoline 25 ~ 30%, carbon disulfide 12 ~ 17%, carbon tetrachloride 2 ~ 6%, chloroform 2 ~ 6%, ring
Ketone 2 ~ 6%;The sum of above-mentioned dosage of each component is 100%;
Or the ink is made of following component mixing: polystyrene 2 ~ 4%, DMF9 ~ 11%, THF9 ~ 11%, rosin 2 ~ 4%,
Beeswax 13 ~ 16%, paraffin 2 ~ 4%, isopropyl ether 4 ~ 6%, methylene chloride 4 ~ 6%, gasoline 18 ~ 22%, carbon disulfide 9 ~ 11%, carbon tetrachloride
9 ~ 11%, chloroform 1 ~ 2%, cyclohexanone 1 ~ 2%, kerosene 2 ~ 4%, turpentine oil 1 ~ 2%;The sum of above-mentioned dosage of each component is 100%;
Or the ink is made of following component mixing: stearic acid 30 ~ 36%, 15 ~ 20%, 46 DEG C of paraffin 9 ~ 15% of benzene, isopropyl
Ether 7 ~ 11%, carbon disulfide 15 ~ 20%, chloroform 15 ~ 20%;The sum of above-mentioned dosage of each component is 100%.
It under external force can be by the bottom of ware body before the abjection of rectangle frame trace line chase, first with recessed along sharp keen cutter
Ditch is drawn, is hooked, and then adds to be deviate from the single hole slot bottom of ware body with external force in ware bottom surface.
The immunohistochemistry pen includes pen container 5, the spongioid cylindrical body pen core 6 of setting in pen container, is perfused with oil in pen container
Wooden pen core 10 is arranged in ink, spongioid cylindrical body pen core front end;Spongioid cylindrical body pen core is placed in pen container, and pen container is
Cuboid or cylindrical body, it is identical that pen container mouth outside has screw thread to match with the internal screw thread of interior pen cap 7;Stainless shot 8 is placed in pen container
One, make the effect for mixing ink when rocking pen container;The interior pen cap plays closing pen container mouth, prevents ink volatilization from keeping its liquid phase
State and the effect for coupling outer pen cap, inner surface have the internal screw thread closed with pen container tone, can spiral cover pen container mouth, interior pen cap outside
Surface is recessed equipped with identical fore shaft is matched with outer 9 inner lip of pen cap, and cylindrical body pen core end side is equipped with ink brush 11;Outer pen cap
To be equipped with fore shaft inner lip week recessed, match and coincide with interior pen cap outer lip, act pen cap and fixed interior pen cap in closing and be integrated out
Pen container mouth is opened, and plays a part of to propose ink brush.Ink is set on interior pen cap and exchanges hole 12.
One angle of porous room culture dish is in bevel-faced form, facilitates bearing mark;An angle for cultivating ware lid is in and culture
The bevel-faced form that ware cooperates in the angle on inclined-plane;Cultivate interleave depth >=10mm of ware lid and culture dish previous anastomotic periphery.
Claims (2)
1. a kind of group pen that service speed is fast is applied in the detection of cell climbing sheet immunohistochemistry, it is characterized in that: by cell inoculation
Cell climbing sheet is carried out in glass slide formula culture dish, immunocyte histochemical stain identification is carried out after cell culture;Institute
Glass slide formula culture dish is stated equipped with culture dish body and ware lid, culture dish figure number is divided to two kinds of single hole room and porous room;Each pore chamber face
Product is greater than glass slide, and ware body bottom is used directly as glass slide, and ware body bottom lateral surface is equipped with corresponding with glass slide size rectangular
Shape frame trace line chase under external force can deviate from the bottom of ware body along rectangle frame trace line chase, and acquisition has culture cell
Face is ready for use on various Immunohistochemical detections just as the ware bottom of glass slide;
The immunocyte histochemical stain identification successively includes the following steps:
(1) 15 min are fixed with ice acetone or 4% paraformaldehyde is fixed;
(2) flowing water rinses, and is cleaned 3 times with PBS;
(3) 10 min are incubated for 0.5%Triton X-100;
(4) 0.3%H2O2It is incubated for 10 min;
(5) after cleaning sample 3 times with PBS, hot wind is sufficiently dry;
(7) ink is drawn with immunohistochemistry pen on cell climbing sheet and divides 2-20 separation dyeing area;
(8) air is sufficiently dry;
(9) it is closed with normal two antiserum and is incubated for 10 min;
(10) mouse is added dropwise respectively in each cell dyeing area or rabbit-anti first antibody is incubated for 30~60 min;
(11) it absorbs each cell dyeing area liquid and is cleaned 3 times with PBS;
(12) enzyme-linked mouse is added dropwise or rabbit-anti secondary antibody working solution is incubated for 30~60 min;
(13) PBS is cleaned 3 times;
(14) DAB develops the color, and is protected from light, under the microscope;
(15) distillation washing;
(16) haematoxylin lining dye;
(17) hydrochloride alcohol breaks up, and originally washes;
(18) aqueous mounting medium mounting;
The ink is made of following component mixing: stearic acid 30 ~ 36%, 15 ~ 20%, 46 DEG C of paraffin 9 ~ 15% of benzene, and isopropyl ether 7 ~
11%, carbon disulfide 15 ~ 20%, chloroform 15 ~ 20%;The sum of above-mentioned dosage of each component is 100%;
The immunohistochemistry pen includes pen container, the spongioid cylindrical body pen core of setting in pen container, is perfused with ink, sponge in pen container
Wooden pen core is arranged in the cylindrical body pen core front end of sample;Spongioid cylindrical body pen core is placed in pen container, pen container be cuboid or
Cylindrical body, it is identical that pen container mouth outside has screw thread to match with the internal screw thread of interior pen cap;Stainless shot one is placed in pen container, rocks pen
Make the effect for mixing ink when cylinder;The interior pen cap plays closing pen container mouth, prevents ink volatilization from keeping its liquid phase state and connection
The effect of outer pen cap, inner surface have the internal screw thread closed with pen container tone, can spiral cover pen container mouth, the outer surface of interior pen cap be equipped with
The identical fore shaft of outer pen cap inner lip matching is recessed, and cylindrical body pen core end side is equipped with ink brush;The inner lip week of outer pen cap sets
Have that fore shaft is recessed, match and coincide with interior pen cap outer lip, rise in closing pen cap and it is fixed in pen cap be integrated and open pen container mouth, and rise
It is proposed the effect of ink brush.
2. the fast group pen of service speed according to claim 1 is applied in the detection of cell climbing sheet immunohistochemistry, special
Sign is: an angle of porous room culture dish is in bevel-faced form, facilitates bearing mark;One angle of culture ware lid is in be in culture dish
The bevel-faced form of the angle cooperation on inclined-plane;Cultivate interleave depth >=10mm of ware lid and culture dish previous anastomotic periphery.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610404345.2A CN105938065B (en) | 2014-09-26 | 2014-09-26 | The fast group pen of service speed is applied in the detection of cell climbing sheet immunohistochemistry |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410500701.1A CN104359741B (en) | 2014-09-26 | 2014-09-26 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
CN201610404345.2A CN105938065B (en) | 2014-09-26 | 2014-09-26 | The fast group pen of service speed is applied in the detection of cell climbing sheet immunohistochemistry |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201410500701.1A Division CN104359741B (en) | 2014-09-26 | 2014-09-26 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
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CN105938065A CN105938065A (en) | 2016-09-14 |
CN105938065B true CN105938065B (en) | 2019-03-12 |
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CN201610404342.9A Active CN106092702B (en) | 2014-09-26 | 2014-09-26 | The group pen for reducing reagent dosage is applied in the detection of cell climbing sheet immunohistochemistry |
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CN106248464B (en) * | 2016-09-02 | 2020-06-09 | 四川大学 | Cell climbing sheet efficient dyeing device |
CN107699486A (en) * | 2017-09-22 | 2018-02-16 | 山东省农业科学院畜牧兽医研究所 | For dyeing the Tissue Culture Dish and cell culture dyeing, observational technique of observation |
CN109100503B (en) * | 2018-09-20 | 2021-02-02 | 同济大学 | Immunohistochemical pen liquid and preparation method thereof |
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