CN103352005B - A kind of culture dish - Google Patents
A kind of culture dish Download PDFInfo
- Publication number
- CN103352005B CN103352005B CN201310326634.1A CN201310326634A CN103352005B CN 103352005 B CN103352005 B CN 103352005B CN 201310326634 A CN201310326634 A CN 201310326634A CN 103352005 B CN103352005 B CN 103352005B
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- China
- Prior art keywords
- clamp
- cover glass
- culture dish
- jiao
- dish body
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Abstract
The invention discloses a kind of culture dish, comprise culture dish body, also comprise the cover glass stationary installation of the bottom being arranged on described culture dish body.In use, put into by cover glass in cover glass stationary installation, by cover glass stationary installation, cover glass and culture dish are realized good fixing, even if cultivating, changing in liquid process to produce and rock, cover glass also can not rock in culture dish.Certainly, cover glass can also be set and pave device, make to reserve certain gap between cover glass and culture dish body, when taking out cover glass, plectrum is inserted in this gap, cover glass can be tilted easily and take out, convenient, safety.
Description
Technical field
The present invention relates to stroma cell culture technique field, particularly a kind of culture dish.
Background technology
Stroma cell is adherent growth, therefore need cell climbing sheet be made when cultivating, namely the cover glass that placement one piece is very thin in advance in the culture dish being added with liquid nutrient medium, so that stroma cell is adherent growth on the cover slip, with pincet, cover glass is taken out after stroma cell covers with cover glass, carry out follow-up test, such as immunohistochemical methods, cell cut, laser co-focusing etc.
In stroma cell culturing process, because culture dish is round, and cover glass is square, after cover glass is put into culture dish, the position of cover glass can not be fixed, to such an extent as in cultivation, change cover glass in liquid process and rock in culture dish, affect culture effect.
Meanwhile, because stroma cell culture environment is liquid culture, namely a certain amount of nutrient solution to be added in culture dish body; The bottom of culture dish body is close to by cover glass after putting into culture dish body, owing to there is surface tension of liquid and adsorptive power, therefore carry out subsequent experimental at needs taking-up cover glass, because the combination of cover glass and culture dish body is too tight, cause cover glass to take out difficulty, cover glass even can be caused to break.
Therefore, how providing a kind of culture dish, to realize the fixing of cover glass, is the current technical issues that need to address of those skilled in the art.
Summary of the invention
The object of this invention is to provide a kind of culture dish, to realize the fixing of cover glass.
For solving the problems of the technologies described above, the invention provides following scheme:
A kind of culture dish, comprises culture dish body, also comprises the cover glass stationary installation of the bottom being arranged on described culture dish body.
Preferably, above-mentioned cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide between described first clamp and described second clamp.
Preferably, above-mentioned first clamp is left clamp, and described second clamp is right clamp.
Preferably, above-mentioned first clamp is upper clamp, and described second clamp is lower clamp.
Preferably, above-mentioned culture dish also comprises the 3rd clamp and the 4th clamp, described 3rd clamp and described 4th clamp have the position of described containment cap slide, described first clamp is left clamp, described second clamp is right clamp, described 3rd clamp is upper clamp, and described 4th clamp is lower clamp.
Preferably, the contact length of above-mentioned first clamp, described second clamp, described 3rd clamp and described 4th clamp and described cover glass is all less than the length of side of described cover glass.
Preferably, above-mentioned cover glass stationary installation comprise first jiao of clamp and with described first jiao of clamp tiltedly to the second jiao of clamp arranged, there is between described first jiao of clamp and described second jiao of clamp the position of containment cap slide.
Preferably, above-mentioned culture dish also comprise triangle clamp and with described triangle clamp tiltedly to the 4th jiao of clamp arranged, described 3rd clamp and described 4th clamp have the position of described containment cap slide.
Preferably, above-mentioned culture dish also comprises cover glass and paves device, and what described cover glass paved device paves the area that area is less than cover glass.
Preferably, above-mentioned cover glass paves device is square boss.
The culture dish that the invention described above provides, comprises culture dish body, also comprises the cover glass stationary installation of the bottom being arranged on described culture dish body.In use, put into by cover glass in cover glass stationary installation, by cover glass stationary installation, cover glass and culture dish are realized good fixing, even if cultivating, changing in liquid process to produce and rock, cover glass also can not rock in culture dish.
Accompanying drawing explanation
The side-looking structural representation of the first embodiment of culture dish that Fig. 1 provides for the embodiment of the present invention;
The plan structure schematic diagram of the first embodiment of culture dish that Fig. 2 provides for the embodiment of the present invention;
The plan structure schematic diagram of culture dish the second embodiment that Fig. 3 provides for the embodiment of the present invention.
In upper Fig. 1-3:
Culture dish body 1, cover glass 2, cover glass pave device 3, right clamp 4, left clamp 5, upper clamp 6, lower clamp 7, first jiao of clamp 8, the 4th jiao of clamp 9, triangle clamp 10, second jiao of clamp 11.
Embodiment
Core of the present invention is to provide a kind of culture dish, to realize the fixing of cover glass.
In order to make those skilled in the art understand the present invention program better, below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
Please refer to Fig. 1, Fig. 2 and Fig. 3, the side-looking structural representation of the first embodiment of culture dish that Fig. 1 provides for the embodiment of the present invention; The plan structure schematic diagram of the first embodiment of culture dish that Fig. 2 provides for the embodiment of the present invention; The plan structure schematic diagram of culture dish the second embodiment that Fig. 3 provides for the embodiment of the present invention.
The culture dish that the embodiment of the present invention provides, comprises culture dish body 1, also comprises the cover glass stationary installation of the bottom being arranged on culture dish body 1.In use, cover glass 2 is put in cover glass stationary installation, by cover glass stationary installation, cover glass 2 and culture dish body 1 are realized good fixing, even if cultivating, changing in liquid process to produce and rock, cover glass 2 also can not rock in culture dish body 1.
Embodiment one:
Cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide 2 between the first clamp and the second clamp.In culture dish body 1, namely place the up and down or left and right of the position of cover glass 2, first clamp and the second clamp are set, such as, first clamp is left clamp 5, second clamp is right clamp 4, just in time cover glass 2 is blocked in left and right, the effect of fixed cap slide 2 can be played, or first clamp be upper clamp 6, second clamp is lower clamp 7, block cover glass 2 up and down, the effect of fixed cap slide 2 can be played equally, and, first clamp and the second clamp have certain length, limit cover glass 2 left bank or right bank, fixed effect is very good.
Can certainly arrange four clamps, i.e. the first clamp, the second clamp, the 3rd clamp and the 4th clamp, wherein, the first clamp is left clamp 5, second clamp be right clamp the 4, three clamp is upper clamp 6, and the 4th clamp is lower clamp 7 simultaneously.Thus being all fixed up and down cover glass 2, fixed effect is better, and, four clamps are while fixing cover glass 2, the taking-up of cover glass 2 should not be disturbed, first clamp, the second clamp, the 3rd clamp and the 4th clamp and cover glass 2 have a contact part, the length of this contact part, namely contact length is all less than the length of side of cover glass 2, this makes it possible to reserve some spaces, facilitate plectrum to be inserted between cover glass 2 and culture dish body 1, cover glass 0 is tilted and takes out.
Meanwhile, because stroma cell culture environment is liquid culture, namely a certain amount of nutrient solution to be added in culture dish body 1; The bottom of culture dish body 1 is close to by cover glass 2 after putting into culture dish body 1, owing to there is surface tension of liquid and adsorptive power, therefore carry out subsequent experimental at needs taking-up cover glass 2, because cover glass 2 is too tight with the combination of culture dish body 1, cause cover glass 2 to take out difficulty, cover glass 2 even can be caused to break.Therefore, in the position of placing cover glass 2, a projection is set, namely cover glass paves device, what cover glass paved device paves the area that area is less than cover glass 2, and namely protruding sectional area is less than the position of cover glass 2, like this, certain gap can be reserved between cover glass 2 and culture dish body 1, when taking out cover glass 2, plectrum is inserted in this gap, cover glass 2 can be tilted easily and take out, convenient, safety.
Cover glass paves device, and can be square boss also can be that regular hexagon is protruding, or the projection of other shapes, and the embodiment of the present invention is not specifically limited the shape that cover glass paves device.
Embodiment two:
Cover glass stationary installation comprise first jiao of clamp 8 and with first jiao of clamp 8 tiltedly to the position between the second jiao of clamp arranged, 11, first jiao of clamp 8 and second jiao of clamp 11 with containment cap slide 2.First jiao of clamp 8 is angles for fixed cap slide 2, its structure can be that two long slabs carry out being spliced to form with 90 degree, so just in time hold an angle, and this angle is well fixed, an angle clamp can limit cover glass 2 movement in the two directions, such as, first jiao of clamp 8 is positioned at the upper left corner of the position of placing cover glass 2, cover glass 2 movement upwards and left can be limited like this, second jiao of clamp 11 is positioned at the lower right corner of the position of placing cover glass 2, with first jiao of clamp 8 tiltedly to setting, second jiao of clamp 11 limits cover glass 2 movement and to the right downwards, first jiao of clamp 8 and second jiao of clamp 11 well limit the movement on cover glass 2 four direction, achieve the fixing of cover glass 2.
Certainly, also four angle clamps can be set simultaneously, namely first jiao of clamp, 8, second jiao of clamp 11, triangle clamp 10 and with triangle clamp 10 tiltedly to the 4th jiao of clamp 9 arranged, the 3rd clamp 10 and the 4th clamp 9 have the position of containment cap slide 2.Now, first jiao of clamp, 8, second jiao of clamp 11, triangle clamp 10 and the 4th jiao of clamp 9 surround the position of placing cover glass 2, first jiao of clamp 8 is positioned at the upper left corner of the position of placing cover glass 2, second jiao of clamp 11 is positioned at the lower right corner of the position of placing cover glass 2, triangle clamp 10 is positioned at the lower left corner of the position of placing cover glass 2,4th jiao of clamp 9 is positioned at the upper right corner of the position of placing cover glass 2, is very perfectly fixed by cover glass 2, there will not be movement.
Meanwhile, because stroma cell culture environment is liquid culture, namely a certain amount of nutrient solution to be added in culture dish body 1; The bottom of culture dish body 1 is close to by cover glass 2 after putting into culture dish body 1, owing to there is surface tension of liquid and adsorptive power, therefore carry out subsequent experimental at needs taking-up cover glass 2, because cover glass 2 is too tight with the combination of culture dish body 1, cause cover glass 2 to take out difficulty, cover glass 2 even can be caused to break.Therefore, in the position of placing cover glass 2, a projection is set, namely cover glass paves device, what cover glass paved device paves the area that area is less than cover glass 2, and namely protruding sectional area is less than the position of cover glass 2, like this, certain gap can be reserved between cover glass 2 and culture dish body 1, when taking out cover glass 2, plectrum is inserted in this gap, cover glass 2 can be tilted easily and take out, convenient, safety.
Cover glass paves device, and can be square boss also can be that regular hexagon is protruding, or the projection of other shapes, and the embodiment of the present invention is not specifically limited the shape that cover glass paves device.
Above culture dish provided by the present invention is described in detail.
Apply specific case herein to set forth principle of the present invention and embodiment, the explanation of above embodiment just understands method of the present invention and core concept thereof for helping.It should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention, can also carry out some improvement and modification to the present invention, these improve and modify and also fall in the protection domain of the claims in the present invention.
Claims (3)
1. a culture dish, comprises culture dish body (1), it is characterized in that, also comprises the cover glass stationary installation of the bottom being arranged on described culture dish body (1),
Described cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide (2) between described first clamp and described second clamp,
Also comprise the 3rd clamp and the 4th clamp, described 3rd clamp and described 4th clamp have the position of described containment cap slide,
Described first clamp is left clamp (5), and described second clamp is right clamp (4), and described 3rd clamp is upper clamp (6), and described 4th clamp is lower clamp (7),
The contact length of described first clamp, described second clamp, described 3rd clamp and described 4th clamp and described cover glass (2) is all less than the length of side of described cover glass (2),
Also comprise cover glass and pave device, what described cover glass paved device paves the area that area is less than cover glass (2),
The height of described first clamp, described second clamp, described 3rd clamp and described 4th clamp is higher than described cover glass.
2. culture dish according to claim 1, it is characterized in that, described cover glass stationary installation comprise first jiao of clamp (8) and with described first jiao of clamp (8) tiltedly to the second jiao of clamp (11) arranged, there is between described first jiao of clamp (8) and described second jiao of clamp (11) position of containment cap slide (2).
3. culture dish according to claim 2, it is characterized in that, also comprise triangle clamp (10) and with described triangle clamp (10) tiltedly to the 4th jiao of clamp (9) arranged, described 3rd clamp (10) and described 4th clamp (9) have the position of described containment cap slide (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310326634.1A CN103352005B (en) | 2013-07-30 | 2013-07-30 | A kind of culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310326634.1A CN103352005B (en) | 2013-07-30 | 2013-07-30 | A kind of culture dish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103352005A CN103352005A (en) | 2013-10-16 |
| CN103352005B true CN103352005B (en) | 2015-12-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310326634.1A Expired - Fee Related CN103352005B (en) | 2013-07-30 | 2013-07-30 | A kind of culture dish |
Country Status (1)
| Country | Link |
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| CN (1) | CN103352005B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105938064B (en) * | 2014-09-26 | 2019-03-19 | 南通大学 | The group pen for reducing reagent dosage is applied in the detection of cell climbing sheet immunohistochemistry |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201485463U (en) * | 2009-07-04 | 2010-05-26 | 王玉亮 | Novel cell culture dish |
| CN201999943U (en) * | 2011-04-28 | 2011-10-05 | 黑龙江八一农垦大学 | Petri dish with glass slide boss |
| CN102925354A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Slide culture dish used by slides in two specifications in combination mode |
| CN203393163U (en) * | 2013-07-30 | 2014-01-15 | 中国人民解放军第三军医大学第二附属医院 | Culture dish |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201429730Y (en) * | 2009-07-14 | 2010-03-24 | 王秀霞 | Novel inspection culture glass sheet |
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2013
- 2013-07-30 CN CN201310326634.1A patent/CN103352005B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201485463U (en) * | 2009-07-04 | 2010-05-26 | 王玉亮 | Novel cell culture dish |
| CN201999943U (en) * | 2011-04-28 | 2011-10-05 | 黑龙江八一农垦大学 | Petri dish with glass slide boss |
| CN102925354A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Slide culture dish used by slides in two specifications in combination mode |
| CN203393163U (en) * | 2013-07-30 | 2014-01-15 | 中国人民解放军第三军医大学第二附属医院 | Culture dish |
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| Publication number | Publication date |
|---|---|
| CN103352005A (en) | 2013-10-16 |
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Granted publication date: 20151223 Termination date: 20170730 |