CN103352005A

CN103352005A - Culture dish

Info

Publication number: CN103352005A
Application number: CN2013103266341A
Authority: CN
Inventors: 刘耀; 张曦
Original assignee: Second Affiliated Hospital of TMMU
Current assignee: Second Affiliated Hospital of TMMU
Priority date: 2013-07-30
Filing date: 2013-07-30
Publication date: 2013-10-16
Anticipated expiration: 2033-07-30
Also published as: CN103352005B

Abstract

The invention discloses a culture dish which comprises a culture dish body, and further comprises a cover glass fixing device arranged at the bottom of the culture dish body. When the culture dish is used, cover glass is put in the cover glass fixing device, the cover glass and the culture dish are fixed well by the cover glass fixing device, and even if the culture dish shakes during the cultivation and liquid exchange processes, the cover glass cannot shake in the culture dish. Of course, a cover glass underlay device can be arranged, so that a certain gap is reserved between the cover glass and the culture dish body, and when the cover glass is taken out, a poke sheet is inserted in the gap to turn up and take out the cover glass easily, and the operation is more convenient and safer.

Description

A kind of culture dish

Technical field

The present invention relates to stroma cell culture technique field, particularly a kind of culture dish.

Background technology

Stroma cell is adherent growth, so when cultivating, need make cell climbing sheet, namely be added with cover glass as thin as a wafer of prior placement in the culture dish of liquid nutrient medium, so that stroma cell is adherent growth on cover glass, stroma cell takes out cover glass with pincet after covering with cover glass, carries out follow-up test, for example immunohistochemical methods, the cell cut, laser co-focusing etc.

In the stroma cell culturing process, because culture dish is round, and cover glass is square, after cover glass is put into culture dish, the position of cover glass can not be fixed, to such an extent as to cover glass rocks in culture dish in the liquid process cultivating, change, affects culture effect.

Simultaneously, because the stroma cell culture environment is liquid culture, namely in the culture dish body, to add a certain amount of nutrient solution; Cover glass is put into the bottom of being close to the culture dish body behind the culture dish body, owing to have surface tension of liquid and adsorptive power, carry out subsequent experimental so take out cover glass at needs, because the combination of cover glass and culture dish body is too tight, cause cover glass to take out difficulty, even can cause cover glass to break.

Therefore, how providing a kind of culture dish, to realize the fixing of cover glass, is the present technical issues that need to address of those skilled in the art.

Summary of the invention

The purpose of this invention is to provide a kind of culture dish, to realize the fixing of cover glass.

For solving the problems of the technologies described above, the invention provides following scheme:

A kind of culture dish comprises the culture dish body, also comprises the cover glass stationary installation of the bottom that is arranged on described culture dish body.

Preferably, above-mentioned cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide between described the first clamp and described the second clamp.

Preferably, above-mentioned the first clamp is left clamp, and described the second clamp is right clamp.

Preferably, above-mentioned the first clamp is upper clamp, and described the second clamp is lower clamp.

Preferably, above-mentioned culture dish also comprises the 3rd clamp and the 4th clamp, described the 3rd clamp and described the 4th clamp have the position of described containment cap slide, described the first clamp is left clamp, described the second clamp is right clamp, described the 3rd clamp is upper clamp, and described the 4th clamp is lower clamp.

Preferably, the contact length of above-mentioned the first clamp, described the second clamp, described the 3rd clamp and described the 4th clamp and described cover glass is all less than the length of side of described cover glass.

Preferably, above-mentioned cover glass stationary installation comprise first jiao of clamp and with the oblique second jiao of clamp to arranging of described first jiao of clamp, have the position of containment cap slide between described first jiao of clamp and the described second jiao of clamp.

Preferably, above-mentioned culture dish also comprise the third angle clamp and with oblique the 4th jiao of clamp to arranging of described third angle clamp, described the 3rd clamp and described the 4th clamp have the position of described containment cap slide.

Preferably, above-mentioned culture dish comprises that also cover glass paves device, and what described cover glass paved device paves area less than the area of cover glass.

Preferably, to pave device be square boss to above-mentioned cover glass.

The culture dish that the invention described above provides comprises the culture dish body, also comprises the cover glass stationary installation of the bottom that is arranged on described culture dish body.In use, cover glass is put in the cover glass stationary installation, by the cover glass stationary installation cover glass and culture dish are realized good fixingly, even rock cultivating, change to produce in the liquid process, cover glass can not rock in culture dish yet.

Description of drawings

The side-looking structural representation of culture dish the first embodiment that Fig. 1 provides for the embodiment of the invention;

The plan structure synoptic diagram of culture dish the first embodiment that Fig. 2 provides for the embodiment of the invention;

The plan structure synoptic diagram of culture dish the second embodiment that Fig. 3 provides for the embodiment of the invention.

Among upper Fig. 1-3:

Culture dish body 1, cover glass 2, cover glass pave device 3, right clamp 4, left clamp 5, upper clamp 6, lower clamp 7, first jiao of clamp 8, the 4th jiao of clamp 9, third angle clamp 10, second jiao of clamp 11.

Embodiment

Core of the present invention provides a kind of culture dish, to realize the fixing of cover glass.

In order to make those skilled in the art understand better the present invention program, the present invention is described in further detail below in conjunction with the drawings and specific embodiments.

Please refer to Fig. 1, Fig. 2 and Fig. 3, the side-looking structural representation of culture dish the first embodiment that Fig. 1 provides for the embodiment of the invention; The plan structure synoptic diagram of culture dish the first embodiment that Fig. 2 provides for the embodiment of the invention; The plan structure synoptic diagram of culture dish the second embodiment that Fig. 3 provides for the embodiment of the invention.

The culture dish that the embodiment of the invention provides comprises culture dish body 1, also comprises the cover glass stationary installation of the bottom that is arranged on culture dish body 1.In use, cover glass 2 is put in the cover glass stationary installation, by the cover glass stationary installation cover glass 2 and culture dish body 1 are realized good fixingly, even rock cultivating, change to produce in the liquid process, cover glass 2 can not rock in culture dish body 1 yet.

Embodiment one:

The cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide 2 between the first clamp and the second clamp.Namely in culture dish body 1, place the up and down or left and right of the position of cover glass 2, the first clamp and the second clamp are set, for example, the first clamp is left clamp 5, the second clamp is right clamp 4, block cover glass 2 just in time, can play the effect of fixed cap slide 2, or the first clamp is that upper clamp 6, the second clamps are lower clamp 7, block up and down cover glass 2, can play equally the effect of fixed cap slide 2, and the first clamp and the second clamp are to have certain length, limit cover glass 2 left banks or right bank, fixed effect is very good.

Four clamps can certainly be set simultaneously, i.e. the first clamp, the second clamp, the 3rd clamp and the 4th clamp, wherein, the first clamp is that left clamp 5, the second clamps are that right clamp 4, the three clamps are that upper clamp 6, the four clamps are lower clamp 7.Thereby to all being fixed up and down of cover glass 2, fixed effect is better, and, four clamps are when fixing cover glass 2, should not disturb the taking-up of cover glass 2, the first clamp, the second clamp, the 3rd clamp and the 4th clamp and cover glass 2 have a contact part, the length of this contact part, be that contact length is all less than the length of side of cover glass 2, so just can reserve some spaces, make things convenient for plectrum to be inserted between cover glass 2 and the culture dish body 1, with cover glass 0 perk and taking-up.

Simultaneously, because the stroma cell culture environment is liquid culture, namely in culture dish body 1, to add a certain amount of nutrient solution; Cover glass 2 is put into the bottom of being close to culture dish body 1 behind the culture dish body 1, owing to have surface tension of liquid and adsorptive power, carry out subsequent experimental so take out cover glass 2 at needs, because cover glass 2 is too tight with the combination of culture dish body 1, cause cover glass 2 to take out difficulty, even can cause cover glass 2 to break.Therefore, in the position of placing cover glass 2 projection is set, be that cover glass paves device, what cover glass paved device paves area less than the area of cover glass 2, and namely the sectional area of projection is less than the position of cover glass 2, like this, can between cover glass 2 and culture dish body 1, reserve certain gap, when taking out cover glass 2 plectrum is inserted in this gap, can and takes out convenient, safety with easily perk of cover glass 2.

It can be that square boss also can be regular hexagon projection that cover glass paves device, the perhaps projection of other shapes, and the embodiment of the invention is not specifically limited the shape that cover glass paves device.

Embodiment two:

The cover glass stationary installation comprise first jiao of clamp 8 and with first jiao of clamp 8 tiltedly to having the position of containment cap slide 2 between 11, the first jiaos of clamps 8 of second jiao of clamp of arranging and the second jiao of clamp 11.First jiao of clamp 8 is for an angle of fixed cap slide 2, its structure can be spliced to form with 90 degree for two long slabs, so just in time hold an angle, and this angle well fixed, an angle clamp can limit the movement of cover glass 2 on both direction, for example, first jiao of clamp 8 is positioned at the upper left corner of the position of placing cover glass 2, can limit like this cover glass 2 makes progress and movement left, second jiao of clamp 11 is positioned at the lower right corner of the position of placing cover glass 2, with first jiao of clamp 8 tiltedly to arranging, second jiao of clamp 11 limits cover glass 2 downwards and movement to the right, first jiao of clamp 8 and second jiao of clamp 11 have well limited the movement on cover glass 2 four directions, have realized the fixing of cover glass 2.

Certainly, also four angle clamps can be set simultaneously, i.e. first jiao of clamp 8, second jiao of clamp 11, third angle clamp 10 and the position that tiltedly the 4th jiao of clamp 9, the three clamp 10 arranging and the 4th clamp 9 had containment cap slide 2 with third angle clamp 10.At this moment, first jiao of clamp 8, second jiao of clamp 11, third angle clamp 10 and the 4th jiao of clamp 9 surround the position of placing cover glass 2, first jiao of clamp 8 is positioned at the upper left corner of the position of placing cover glass 2, second jiao of clamp 11 is positioned at the lower right corner of the position of placing cover glass 2, third angle clamp 10 is positioned at the lower left corner of the position of placing cover glass 2, the 4th jiao of clamp 9 is positioned at the upper right corner of the position of placing cover glass 2, very perfectly cover glass 2 fixed, and can not occur mobile.

More than culture dish provided by the present invention is described in detail.

Used specific case herein principle of the present invention and embodiment are set forth, the explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.

Claims

1. a culture dish comprises culture dish body (1), it is characterized in that, also comprises the cover glass stationary installation of the bottom that is arranged on described culture dish body (1).

2. culture dish according to claim 1 is characterized in that, described cover glass stationary installation comprises the first clamp and the second clamp, has the position of containment cap slide (2) between described the first clamp and described the second clamp.

3. culture dish according to claim 2 is characterized in that, described the first clamp is left clamp (5), and described the second clamp is right clamp (4).

4. culture dish according to claim 2 is characterized in that, described the first clamp is upper clamp (6), and described the second clamp is lower clamp (7).

5. culture dish according to claim 2 is characterized in that, also comprises the 3rd clamp and the 4th clamp, and described the 3rd clamp and described the 4th clamp have the position of described containment cap slide,

Described the first clamp is left clamp (5), and described the second clamp is right clamp (4), and described the 3rd clamp is upper clamp (6), and described the 4th clamp is lower clamp (7).

6. culture dish according to claim 5 is characterized in that, the contact length of described the first clamp, described the second clamp, described the 3rd clamp and described the 4th clamp and described cover glass (2) is all less than the length of side of described cover glass (2).

7. culture dish according to claim 1, it is characterized in that, described cover glass stationary installation comprise first jiao of clamp (8) and with the oblique second jiao of clamp (11) to arranging of described first jiao of clamp (8), have the position of containment cap slide (2) between described first jiao of clamp (8) and the described second jiao of clamp (11).

8. culture dish according to claim 7, it is characterized in that, also comprise third angle clamp (10) and with oblique the 4th jiao of clamp (9) to arranging of described third angle clamp (10), described the 3rd clamp (10) and described the 4th clamp (9) have the position of described containment cap slide (2).

9. culture dish according to claim 1 is characterized in that, comprises that also cover glass paves device, and what described cover glass paved device paves area less than the area of cover glass (2).

10. culture dish according to claim 6 is characterized in that, it is square boss that described cover glass paves device.