CN205115497U - Be applied to culture medium that ovum bubble was cultivateed before chamber - Google Patents
Be applied to culture medium that ovum bubble was cultivateed before chamber Download PDFInfo
- Publication number
- CN205115497U CN205115497U CN201520800906.1U CN201520800906U CN205115497U CN 205115497 U CN205115497 U CN 205115497U CN 201520800906 U CN201520800906 U CN 201520800906U CN 205115497 U CN205115497 U CN 205115497U
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- culture dish
- agar
- culture
- layer
- substratum
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Abstract
The utility model discloses a be applied to culture medium that ovum bubble was cultivateed before chamber, this culture medium includes: the culture dish with can approximately close at this culture dish top open -ended lid, its characterized in that, culture dish inside is provided with agar and solidifies the layer, agar solidifies the layer and coats and be stamped the paraffin oil reservoir, and upper surface that agar solidifies the layer is provided with the pit that a plurality of is used for before the holding chamber ovum bubble and culture solution, the degree of depth of pit is less than agar solidifies the thickness on layer. The utility model discloses utilizing agar to have certain support resilience effect, can ensureing that the base film is complete, circular structure is maintain to the ovum bubble, and the growth of can not overflowing of oocyte and granular cell is convenient for carry on long -term cultivation and is observed and measure, and on the other hand is convenient for add and is changed the culture solution.
Description
Technical field
The utility model belongs to middle Bioexperiment fixture fields, particularly relates to a kind of substratum being applied to follicle culture before chamber.
Background technology
Preantral follicles of animals is placed in culture dish and cultivates by usual laboratory, and culture dish that market is sold maintains drops for making nutrient solution in bottom, all carries out wrapping by adhesion process.Cultivate drop if directly put into by ovarian follicle before chamber to cultivate, easily make basement membrane cell paste diapire growth, cause basement membrane to break, destroy ovarian follicle form, granulosa cell and ovocyte effusion, cannot carry out long-term cultivation observation.
Utility model content
For problems of the prior art, the purpose of this utility model is to provide a kind of substratum being applied to follicle culture before chamber.
In order to achieve the above object, the utility model is a kind of be applied to chamber before the substratum of follicle culture comprise: culture dish and the lid that can cover at this culture dish upper opening, described culture dish inside is provided with agar solidification layer, described agar solidification layer is coated with paraffin oil reservoir, the upper surface of agar solidification layer is provided with several pits for ovarian follicle before accommodating cavity and nutrient solution, and the degree of depth of described pit is less than the thickness of described agar solidification layer.
Further, the diameter of described culture dish is 3.5cm.
Further, the thickness of described agar solidification layer is the half of culture dish depth of inner cavity.
Further, the thickness of described paraffin oil reservoir is 1mm-2mm.
Further, described pit is evenly arranged at the upper surface of described agar solidification layer.
The utility model is a kind of be applied to chamber before the advantage of substratum of follicle culture have: 1. agar has certain support rebound effect, can guarantee that basement membrane is complete, ovarian follicle maintains circular configuration, and ovocyte and granulosa cell can not be overflowed growth, is convenient to carry out long-term cultivation observation; 2. adopt and pit is set and the closed mode covering paraffin oil reservoir on pit, but not the method for totally-enclosed embedding, be convenient to examine under a microscope form on the one hand, measure diameter, on the other hand, so that change liquid, can ensure as before chamber, follicular development provides sufficient nutrient.
Accompanying drawing explanation
Fig. 1 is a kind of structural representation being applied to the substratum of follicle culture before chamber of the present utility model;
Fig. 2 is the vertical view of culture dish in Fig. 1.
Embodiment
Below in conjunction with accompanying drawing, describe embodiment of the present utility model in detail.
The specific embodiment of the substratum of follicle culture before chamber is applied to as shown in Figure 1 and Figure 2 for the utility model is a kind of, comprise in the present embodiment: culture dish 2 and lid 1, the diameter of culture dish 2 is 3.5cm, and the top of culture dish 2 is provided with opening, and the opening of culture dish 2 can cover by lid 1.Culture dish 2 inside is provided with agar solidification layer 4, the thickness of agar solidification layer 4 is the half of culture dish 2 depth of inner cavity, agar solidification layer 4 is coated with the paraffin oil reservoir 3 that thickness is 1mm-2mm, the upper surface of agar solidification layer 4 is provided with 5 pits 6, these 5 pits 6 are evenly arranged at the upper surface of agar solidification layer 4, be equipped with ovarian follicle 5 and nutrient solution 4 before chamber in pit 6, the degree of depth of pit 6 is less than the thickness of agar solidification layer 4.
Of the present utility model a kind of be applied to chamber before the making processes of substratum of follicle culture as follows: in 3.5cm culture dish, pour into agar, circular aperture is dug with aseptic pocket knife after agar solidification, not penetrating to bottom, follicle culture liquid before chamber is added in aperture, put into ovarian follicle before chamber, cover paraffin oil thereon afterwards, build culture dish lid, put into incubator and cultivate.
The utility model is a kind of be applied to chamber before the substratum of follicle culture there are several advantages below:
1. agar has certain support rebound effect, can guarantee that basement membrane is complete, and ovarian follicle maintains circular configuration, and ovocyte and granulosa cell can not be overflowed growth, is convenient to carry out long-term cultivation observation;
2. adopt and pit is set and the closed mode covering paraffin oil reservoir on pit, but not the method for totally-enclosed embedding, be convenient to examine under a microscope form on the one hand, measure diameter, on the other hand, so that change liquid, can ensure as before chamber, follicular development provides sufficient nutrient.
Above-mentioned example is just for illustration of the utility model, and embodiment of the present utility model is not limited to these examples, and what those skilled in the art made meets the various embodiments of the utility model thought all within protection domain of the present utility model.
Claims (5)
1. one kind is applied to the substratum of follicle culture before chamber, this substratum comprises: culture dish and the lid that can cover at this culture dish upper opening, it is characterized in that, described culture dish inside is provided with agar solidification layer, described agar solidification layer is coated with paraffin oil reservoir, the upper surface of agar solidification layer is provided with several pits for ovarian follicle before accommodating cavity and nutrient solution, and the degree of depth of described pit is less than the thickness of described agar solidification layer.
2. substratum as claimed in claim 1, it is characterized in that, the diameter of described culture dish is 3.5cm.
3. substratum as claimed in claim 1, it is characterized in that, the thickness of described agar solidification layer is the half of culture dish depth of inner cavity.
4. substratum as claimed in claim 1, it is characterized in that, the thickness of described paraffin oil reservoir is 1mm-2mm.
5. substratum as claimed in claim 1, it is characterized in that, described pit is evenly arranged at the upper surface of described agar solidification layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520800906.1U CN205115497U (en) | 2015-10-13 | 2015-10-13 | Be applied to culture medium that ovum bubble was cultivateed before chamber |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520800906.1U CN205115497U (en) | 2015-10-13 | 2015-10-13 | Be applied to culture medium that ovum bubble was cultivateed before chamber |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205115497U true CN205115497U (en) | 2016-03-30 |
Family
ID=55571120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520800906.1U Expired - Fee Related CN205115497U (en) | 2015-10-13 | 2015-10-13 | Be applied to culture medium that ovum bubble was cultivateed before chamber |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205115497U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129402A (en) * | 2019-04-15 | 2019-08-16 | 宁夏医科大学 | A kind of research method that Rho-ROCK signal pathway inhibitor Y27632 influences mouse eggs bubble ectogenesis |
-
2015
- 2015-10-13 CN CN201520800906.1U patent/CN205115497U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110129402A (en) * | 2019-04-15 | 2019-08-16 | 宁夏医科大学 | A kind of research method that Rho-ROCK signal pathway inhibitor Y27632 influences mouse eggs bubble ectogenesis |
| CN110129402B (en) * | 2019-04-15 | 2023-08-01 | 宁夏医科大学 | Method for researching influence of Rho-ROCK signal channel inhibitor Y27632 on mouse follicle in-vitro development |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160330 Termination date: 20161013 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |