CN203324275U - Immunochemical staining water-blocking device, cell culture dish and cell culture plate - Google Patents
Immunochemical staining water-blocking device, cell culture dish and cell culture plate Download PDFInfo
- Publication number
- CN203324275U CN203324275U CN2013202563878U CN201320256387U CN203324275U CN 203324275 U CN203324275 U CN 203324275U CN 2013202563878 U CN2013202563878 U CN 2013202563878U CN 201320256387 U CN201320256387 U CN 201320256387U CN 203324275 U CN203324275 U CN 203324275U
- Authority
- CN
- China
- Prior art keywords
- blocking ring
- water blocking
- waterproofing device
- blocking device
- annular water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses an immunochemical staining water-blocking device. The immunochemical staining water-blocking device comprises an annular water-blocking ring, wherein a groove is annularly formed in the bottom surface of the annular water-blocking ring. The utility model also discloses a cell culture dish and a cell culture plate containing the immunochemical staining water-blocking device. By virtue of the immunochemical staining water-blocking device, on the premise that an experiment result is not influenced, the usage amount of antigen-antibodies and reagents can be reduced to the greatest extent, sheet drying and release caused by flowing and diffusion of liquid and loss of surface reagents during staining are avoided, and the experiment efficiency can be improved while the staining effect is ensured. The immunochemical staining water-blocking device is applicable to histochemistry and fluorescence in-situ hybridization tests on a glass slide, and can be applied to all cytochemistry, histochemistry and in-situ hybridization tests on the culture dish, the culture plate or the glass slide.
Description
Technical field
The utility model belongs to Biochemistry Experiment device technique field, and the instrument that is blocked water while relating to cytochemistry and histochemical stain and process is specifically related to a kind of immunochemistry dyeing waterproofing device, Tissue Culture Dish and Tissue Culture Plate.
Background technology
The disposal route that blocks water that existing immunochemistry dyeing adopts mostly is and adopts SABC pen (the SABC pen is again Pap Pen, PAP PEN-pen, DAKO PEN-pen, immunohistochemistry PAP pen, super SABC pen, PAP pen, SuperPap Pen).Various immunohistochemical staining tests for the section of immunostaining experiment front glass, as PAP method, ABC method, immunofluorescence technique, frozen section and hybridization in situ technique, to the tissue that the is fixed in microslide processing that blocked water, can under the prerequisite that does not affect experimental result, effectively reduce antigen-antibody and using amount of reagent, and liquid trickling and diffusion and surface reagent run off and cause while avoiding dyeing dry plate, flake, improve operating speed) draw a circle going on the microslide of experiment in advance.Then, then on the microslide after sample is affixed on to this and draws a circle.Because this SABC pen is to utilize the nib device to draw a circle on slide the liquid material water-proof material, after drawing a circle, needing to wait for a period of time allows a liquid drying.Like this, the common attached cells cultivating in double dish etc. are after paraformaldehyde is fixing, can't be directly used in and draw a circle and continue subsequent experimental, but need to wait for a period of time, allow cell drying at the bottom of double dish could use SABC stroke circle, the working fluid that allows a liquid drying just can add other that then also needs to wait for a period of time continues experiment.
The shortcoming of this disposal route is, be difficult to control a speed of liquid output in practical operation, etc. temporal delaying to be dried, dry run causes the possible change of some antigenic component and loss, the liquid composition harmful effect possible to experimental result, and draw a circle dry after the water blocking ring uneven thickness cause block-water effect not good, or the too little antibody clean result that makes of water blocking ring is bad etc.
The utility model content
The utility model is intended to overcome the deficiencies in the prior art, and a kind of immunochemistry dyeing waterproofing device is provided.Be mainly used in the subregion of same double dish inner cell chemical staining.
In order to achieve the above object, the technical scheme that the utility model provides is:
Described immunochemistry dyeing waterproofing device comprises annular water blocking ring, and described annular water blocking ring bottom surface is equipped with groove.
Preferably, be provided with magnet ring in described groove; Described immunochemistry dyeing waterproofing device also comprises geometrical clamp; Described annular water blocking ring surrounding is provided with the geometrical clamp draw-in groove; The annular distance of described annular water blocking ring is divided into some zones by water baffle bar; Described annular water blocking ring is elastomeric material; Described water baffle bar is elastomeric material.
The utility model also provides a kind of Tissue Culture Dish, and this Tissue Culture Dish inner bottom surface is provided with above-mentioned immunochemistry dyeing waterproofing device.
The utility model also provides a kind of Tissue Culture Plate, and the hole bottom surface of this Tissue Culture Plate is provided with the described immunochemistry dyeing of claim 1 to 6 any one waterproofing device.
Below in conjunction with design concept, the utility model is described in further detail:
The utility model adopts bottom surface to be equipped with the annular rubber ring of groove as water blocking ring, when the immunochemistry Coloration experiment, this water blocking ring is positioned over to the bottom interior surface of slide upper surface, double dish or culture plate hole, firmly push water blocking ring, make the Bas Discharged in groove, water blocking ring just can be adsorbed on the bottom interior surface that is positioned over slide upper surface, double dish or culture plate hole.
As a kind of improvement, the utility model arranges magnet ring in groove, when the immunochemistry Coloration experiment, an annular water blocking ring is positioned over to the bottom interior surface of slide upper surface, double dish or culture plate hole; Another annular water blocking ring is placed in to the outer bottom outer surface of slide lower surface, double dish or culture plate hole simultaneously, and make it the annular water blocking ring corresponding (attracting each other) with slide upper surface, double dish bottom interior surface, culture plate hole bottom interior surface, thereby stationary annular water blocking ring.
Further, immunochemistry dyeing waterproofing device of the present utility model also comprises geometrical clamp, and in annular water blocking ring surrounding, the geometrical clamp draw-in groove is set.An annular water blocking ring is positioned over to the bottom interior surface of slide upper surface, double dish or culture plate hole; Another annular water blocking ring is placed in to the outer bottom outer surface of slide lower surface, double dish or culture plate hole simultaneously, and makes it corresponding with the annular water blocking ring of slide upper surface, double dish bottom interior surface, culture plate hole bottom interior surface.Then geometrical clamp is clamped to two annular water blocking ring, the termination of geometrical clamp snaps in the geometrical clamp draw-in groove.
Take that the cell culture material of commonly using---the 35mm Tissue Culture Dish is example, waterproofing device of the present utility model is for separating the zone in same double dish, makes rare cell or tissue sample can carry out the dyeing of two kinds and two or more antibody simultaneously.
In kind, have in the 35mm ware of cell, cell need to carry out the staining analysis of 3 kinds of antigens, and cell material is very valuable, and temperature and humidity is very large on the cellular morphology impact.Traditional SABC pen need to wait the double dish drying, can't under moisture state, draw a circle, even and the rear block-water effect of drawing a circle not good yet.3 of this waterproofing devices (external diameter 14mm, height 10mm) can directly be positioned in the 35mm ware, and the absorption affinity by magnet is fixed in the culture plate bottom.Then fix this circle (clip that design concept is used with the clothing that dries in the air) with 2 outer geometrical clamp pressurizations.After this can be in the situation that without drying such as ware such as grade, it be being carried out to the staining analysis of Multiple Antibodies in same ware.And, when washing, can add more damping fluid fully to wash.After washing primary antibodie, add same two when anti-, can remove this instrument that blocks water.Can be conducive to like this two anti-evenly hatching and fully washing.
For 2 kinds and following antigen dyeing, suggestion adopts independent water blocking ring effect, because water blocking ring internal diameter relative length now still is convenient to operate in the 35mm ware.For the dyeing of 4 kinds of antigens, can adopt annular water blocking ring annular distance to be divided into the matrix pattern water blocking ring (external diameter 32mm, height 10mm) in four zones by water baffle bar.In larger double dish, carry out the dyeing of antigen more than four kinds and adopt matrix pattern water blocking ring and mode that the water blocking ring circle of subregion combines to carry out, or be divided into the more water blocking ring of multizone with annular water blocking ring annular distance by water baffle bar and carry out.
The utility model is under the prerequisite that does not affect experimental result, can reduce to greatest extent antigen-antibody and using amount of reagent, and liquid trickling and diffusion and surface reagent run off and cause while avoiding dyeing dry plate, flake, more can improve conventional efficient when guaranteeing Color.
The utility model is same applicable for the histochemistry carried out on microslide and fluorescence in situ hybridization test.All cytochemistries of carrying out on double dish, plate or slide, histochemistry, In situ hybridization be applicable waterproofing device of the present utility model all.
The accompanying drawing explanation
The upward view that Fig. 1 is a kind of embodiment of the utility model waterproofing device;
The upward view that Fig. 2 is the another kind of embodiment of the utility model waterproofing device;
The upward view that Fig. 3 is another embodiment of the utility model waterproofing device;
The sectional view that Fig. 4 is a kind of embodiment of the utility model Tissue Culture Dish;
The vertical view that Fig. 5 is a kind of embodiment of the utility model Tissue Culture Plate.
In figure: 1, annular water blocking ring; 2, groove; 3, magnet ring; 4, water baffle bar; 5, geometrical clamp; 6, fixed card slot; 7, Tissue Culture Dish; 8, Tissue Culture Plate.
Embodiment
Referring to Fig. 1, described immunochemistry dyeing waterproofing device comprises annular water blocking ring 1, and described annular water blocking ring 1 bottom surface is equipped with groove 2, and described annular water blocking ring 1 is elastomeric material.
Referring to Fig. 1 and 2, described immunochemistry dyeing waterproofing device comprises annular water blocking ring 1, and described annular water blocking ring 1 bottom surface is equipped with groove 2, in described groove 2, is provided with magnet ring 3, and described annular water blocking ring 1 is elastomeric material.
Embodiment 3
Referring to Fig. 1 and 3, described immunochemistry dyeing waterproofing device comprises annular water blocking ring 1, described annular water blocking ring 1 bottom surface is equipped with groove 2, and the annular distance of described annular water blocking ring 1 is divided into 4 zones by water baffle bar 4, and described annular water blocking ring 1 and water baffle bar 4 are elastomeric material.
Referring to Fig. 2 and 4, described Tissue Culture Dish 7 inner bottom surfaces are provided with immunochemistry dyeing waterproofing device, described immunochemistry dyeing waterproofing device comprises annular water blocking ring 1, described annular water blocking ring 1 bottom surface is equipped with groove 2, be provided with magnet ring 3 in described groove 2, described immunochemistry dyeing waterproofing device also comprises geometrical clamp 5; Described annular water blocking ring 1 surrounding is provided with geometrical clamp draw-in groove 6, and described annular water blocking ring 1 is elastomeric material.
Embodiment 5
Referring to Fig. 5, the hole bottom surface of described Tissue Culture Plate 8 is provided with the described immunochemistry dyeing of embodiment 1 to 4 any one waterproofing device.
Claims (8)
1. an immunochemistry dyeing waterproofing device, is characterized in that, it comprises annular water blocking ring (1), and described annular water blocking ring (1) bottom surface is equipped with groove (2).
2. immunochemistry dyeing waterproofing device as claimed in claim 1, is characterized in that, is provided with magnet ring (3) in described groove (2).
3. immunochemistry dyeing waterproofing device as claimed in claim 1, is characterized in that, described immunochemistry dyeing waterproofing device also comprises geometrical clamp (5); Described annular water blocking ring (1) surrounding is provided with geometrical clamp draw-in groove (6).
4. immunochemistry dyeing waterproofing device as claimed in claim 1, is characterized in that, the annular distance of described annular water blocking ring (1) is divided into some zones by water baffle bar (4).
5. immunochemistry dyeing waterproofing device as claimed in claim 1, is characterized in that, described annular water blocking ring (1) is elastomeric material.
6. immunochemistry dyeing waterproofing device as claimed in claim 4, is characterized in that, described water baffle bar (4) is elastomeric material.
7. a Tissue Culture Dish, is characterized in that, described Tissue Culture Dish inner bottom surface is provided with the described immunochemistry dyeing of claim 1 to 6 any one waterproofing device.
8. a Tissue Culture Plate, is characterized in that, the hole bottom surface of described Tissue Culture Plate is provided with the described immunochemistry dyeing of claim 1 to 6 any one waterproofing device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013202563878U CN203324275U (en) | 2013-05-13 | 2013-05-13 | Immunochemical staining water-blocking device, cell culture dish and cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013202563878U CN203324275U (en) | 2013-05-13 | 2013-05-13 | Immunochemical staining water-blocking device, cell culture dish and cell culture plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203324275U true CN203324275U (en) | 2013-12-04 |
Family
ID=49663613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013202563878U Expired - Lifetime CN203324275U (en) | 2013-05-13 | 2013-05-13 | Immunochemical staining water-blocking device, cell culture dish and cell culture plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203324275U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837592A (en) * | 2014-03-18 | 2014-06-04 | 中国计量科学研究院 | Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry) |
| CN104359741A (en) * | 2014-09-26 | 2015-02-18 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN104513784A (en) * | 2014-12-15 | 2015-04-15 | 成都红柿子科技有限公司 | Petri dish rotating apparatus |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
-
2013
- 2013-05-13 CN CN2013202563878U patent/CN203324275U/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837592A (en) * | 2014-03-18 | 2014-06-04 | 中国计量科学研究院 | Method for in-situ measuring iron content in biological tissue sample by adopting isotopic dilution LA-ICP-MS (laser ablation inductively coupled plasma mass spectrometry) |
| CN104359741A (en) * | 2014-09-26 | 2015-02-18 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN105938064A (en) * | 2014-09-26 | 2016-09-14 | 南通大学 | Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides |
| CN105938065A (en) * | 2014-09-26 | 2016-09-14 | 南通大学 | Application of Pap pen with fast operation speed in cell growing slide immunohistochemical detection |
| CN104359741B (en) * | 2014-09-26 | 2017-01-11 | 南通大学 | Application of multi-functional PAP pen in cell climbing immunohistochemical detection |
| CN105938065B (en) * | 2014-09-26 | 2019-03-12 | 南通大学 | The fast group pen of service speed is applied in the detection of cell climbing sheet immunohistochemistry |
| CN105938064B (en) * | 2014-09-26 | 2019-03-19 | 南通大学 | The group pen for reducing reagent dosage is applied in the detection of cell climbing sheet immunohistochemistry |
| CN104513784A (en) * | 2014-12-15 | 2015-04-15 | 成都红柿子科技有限公司 | Petri dish rotating apparatus |
| CN106248464A (en) * | 2016-09-02 | 2016-12-21 | 四川大学 | A kind of efficient dyeing apparatus of cell climbing sheet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203324275U (en) | Immunochemical staining water-blocking device, cell culture dish and cell culture plate | |
| RU2012157128A (en) | METHOD FOR QUANTITATIVE TRANSFER OF ANALYZED SAMPLES | |
| CN211697823U (en) | Colloidal gold kit | |
| CN103743612B (en) | Detachably, the microslide couveuse of volume adjustable and airtight space | |
| CN104359741A (en) | Application of multi-functional PAP pen in cell climbing immunohistochemical detection | |
| CN202757821U (en) | Reserving device for liquid on solid surface | |
| CN110618262A (en) | Glass slide and detection plate matched with same | |
| CN104614516A (en) | Slide incubator ensuring good experimental results | |
| CN206208629U (en) | A kind of immunofluorescent staining slide | |
| CN201740686U (en) | Universal pathological histocyte chemical staining glass slide | |
| CN205426924U (en) | Disposable immunofluorescence detects slide glass | |
| CN202002935U (en) | Kit for detecting staphylococcus aureus infection in blood | |
| CN110501336A (en) | The capsular swelling colouring method of streptococcus pneumonia | |
| CN203825009U (en) | Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody | |
| CN105548123A (en) | Two-color fluorescence labeling method for identifying fission multiplication phase and spore multiplication phase of microsporidia | |
| CN215339892U (en) | Immunochromatography multi-connection reagent card | |
| CN104614517A (en) | Application method of detachable slide incubator | |
| CN103116215A (en) | Novel cell slide glass component based on nitrocellulose membrane and used for exfoliative cytology examination | |
| CN203881769U (en) | Elisa plate sample injector | |
| CN208476787U (en) | A kind of immunofluorescence dyeing kit | |
| CN202013354U (en) | Detection box used for immune chromatography detection technology | |
| CN113092220A (en) | Immunohistochemical wet box | |
| CN206772983U (en) | A kind of vaginitis self-test device | |
| CN217305197U (en) | ELISA plate water bath device | |
| CN204666632U (en) | Immunohistochemical staining device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20131204 |
|
| CX01 | Expiry of patent term |