CN203825009U - Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody - Google Patents
Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody Download PDFInfo
- Publication number
- CN203825009U CN203825009U CN201320699111.7U CN201320699111U CN203825009U CN 203825009 U CN203825009 U CN 203825009U CN 201320699111 U CN201320699111 U CN 201320699111U CN 203825009 U CN203825009 U CN 203825009U
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- China
- Prior art keywords
- bottle
- disease virus
- mouth disease
- box body
- division board
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 13
- 241000700605 Viruses Species 0.000 title abstract 3
- 208000030194 mouth disease Diseases 0.000 title abstract 3
- 241000710198 Foot-and-mouth disease virus Species 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 239000000427 antigen Substances 0.000 claims abstract description 11
- 102000036639 antigens Human genes 0.000 claims abstract description 11
- 108091007433 antigens Proteins 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims abstract description 4
- 241000282898 Sus scrofa Species 0.000 claims description 21
- 230000004308 accommodation Effects 0.000 claims description 18
- 238000004020 luminiscence type Methods 0.000 claims description 9
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 239000012530 fluid Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 4
- 108010003533 Viral Envelope Proteins Proteins 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 150000003016 phosphoric acids Chemical class 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 239000012086 standard solution Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 210000002966 serum Anatomy 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract 3
- 229910019142 PO4 Inorganic materials 0.000 abstract 1
- 230000003139 buffering effect Effects 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 1
- 239000010452 phosphate Substances 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a chemiluminescence reagent kit of a porcine foot-mouth disease virus antibody. The kit comprises a kit body, wherein a micropore plate with 96 pores is arranged in a left containing space of the kit body; a plate sealing film for shielding light wraps the micropore plate with the 96 pores; an immobilized porcine foot-and-mouth disease virus enveloping antigen is arranged at the bottom of the surfaces of the pores of the micropore plate; a reagent bottle base with a plurality of downward concave bottle positions is arranged in a right containing space; one bottle of ovine-against-porcine antibody working liquid marked by horseradish peroxidase, one bottle of concentrated phosphate buffering liquid, one bottle of concentrated washing liquid, one bottle of luminous substrate liquid A, one bottle of luminous substrate liquid B, six bottles of standard product solutions, one bottle of stopping liquid and two bottles of yin-yang contrast working liquids are arranged in the reagent bottle base. The kit has the characteristics of high detection sensitivity, flexible application and full-automatic analysis, and can be used for carrying out quantitative analysis on the porcine foot-mouth disease virus antibody in porcine serum or porcine plasma, thereby being favorable for guiding an immune opportunity so as to provide a guarantee for farmers.
Description
Technical field
The utility model relates to field of immunodetection, specifically relates to a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit.
Background technology
At present, swine foot-and-mouth disease virus detects mainly take that aetology detects and serology detects as main, and enzyme linked immunosorbent assay, fluorescence labeling are tested, fluorescence RT-PCR is for detection of the antibody of swine foot-and-mouth disease virus.
(1) enzyme linked immunosorbent assay
Utilize antigen and antibody capable to be combined with surface of solid phase carriers and can keep its immunity,
Label after antigen or antibody and enzyme conjugates can keep the immunocompetence of antigen or antibody, can keep the activity of enzyme again, the antibody of the antigen in examined object or antibody and solid phase surface or antigen generation immune response are also combined in solid phase surface, this antigen antibody complex again can be in conjunction with corresponding enzyme labeling thing, unconjugated enzyme labeling thing is removed in washing, add enzyme reaction substrate, the process that substrate is coloured product by enzymatic.Shortcoming: length consuming time, complex operation step, cross pollution easily occurs.
(2) fluorescence labeling test
After utilizing fluorchrome to be combined with label, do not affect the immunocompetence of label, can be comparatively firm with specific antigen in sample or antibody specific binding and this combination, while washing with damping fluid, remove after free fluorescein-labelled label, by the immunology tracer technique of fluorescence microscope.Shortcoming: equipment manufacturing cost is higher, diagnosticum is difficult to buy, and is difficult to now popularization and application.Meanwhile, be subject to slide thickness and observing time and observer's experience, the restriction of skill level.
(3) fluorescence RC-PCR
Fluorescence RC-PCR is the new technology growing up late 1990s, and fluorescein-labeled probe is reacted together with primer in luminoscope, and computer carries out Real-Time Monitoring to whole reaction.Shortcoming: operating personnel should wear masks, often changes disposable glove.
Highly sensitive is the superiority of chemiluminescence immune assay key, and easy and simple to handle.Chemiluminescence immune assay can detect the material that the methods such as radiommunoassay and enzyme-linked immuno assay cannot detect.
Utility model content
The purpose of this utility model is the deficiency for solving the problems of the technologies described above, and a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit is provided, highly sensitive, sensing range is wide, easy and simple to handle fast and nontoxic pollution-free.
The utility model is the deficiency solving the problems of the technologies described above, the technical scheme adopting is: a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit, comprise box body, box body is the papery box body with upper cover, described box body is divided into a left side by division board, right two accommodation spaces, division board is perpendicular to the base plate setting of box body, wherein, the volume of left accommodation space is 1/3 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates, 96 hole microwell plates are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates is provided with immobilised swine foot-and-mouth disease virus envelope antigen, described right accommodation space is provided with one with the reagent bottle pedestal of a plurality of recessed bottles position, in reagent bottle pedestal, be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.
On the box wall of the left accommodation space of described box body, be coated with one deck froth bed.
Described division board is plastic material, and the inwall correspondence of box body is provided with two grooves from plate, and division board is plugged in groove.
The thickness of described division board is 2 times of box body thickness, and on division board, the thickness of froth bed is 2 times of division board thickness.
beneficial effect
Kit of the present utility model has that detection sensitivity is high, applying flexible, can carry out the feature of automatical analysis, can carry out quantitative test to swine foot-and-mouth disease virus antibody in pig serum or blood plasma, is beneficial to the immunity opportunity instructed, for raiser provides safeguard.
Accompanying drawing explanation
Fig. 1 is the inner structure schematic diagram after the utility model lid is opened;
Fig. 2 is the structural representation of the utility model reagent bottle pedestal;
Mark in figure: 1, box body, 2, upper cover, 3,96 hole microwell plates, 4, reagent bottle pedestal.
Embodiment
As shown in the figure: a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit, comprise box body 1, box body 1 is the papery box body with upper cover 2, described box body 1 is divided into a left side by division board 2, right two accommodation spaces, division board 2 is perpendicular to the base plate setting of box body 1, wherein, the volume of left accommodation space is 1/3 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates 3, 96 hole microwell plates 3 are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates 3 is provided with immobilised swine foot-and-mouth disease virus envelope antigen, described right accommodation space is provided with one with the reagent bottle pedestal 4 of a plurality of recessed bottles position, in reagent bottle pedestal 4, be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.On the box wall of the left accommodation space of described box body 1, be coated with one deck froth bed.Described division board 2 is plastic material, and the inwall correspondence of box body 1 is provided with two grooves from plate 2, and division board 2 is plugged in groove.The thickness of described division board 2 is 2 times of box body 1 thickness, and on division board 2, the thickness of froth bed is 2 times of division board 2 thickness.Kit of the present utility model has that detection sensitivity is high, applying flexible, can carry out the feature of automatical analysis, can carry out quantitative test to swine foot-and-mouth disease virus antibody in pig serum or blood plasma, be beneficial to the immune opportunity of instructing, for raiser provides safeguard, and box body inner structure is reasonable in design, easy to use, can effectively reduce the damage in transportation or disassembly process, reagent bottle being caused.
Claims (4)
1. a swine foot-and-mouth disease virus antibody chemical luminescence kit, comprise box body (1), box body (1) is the papery box body with upper cover (2), it is characterized in that: described box body (1) is divided into a left side by division board (2), right two accommodation spaces, division board (2) is perpendicular to the base plate setting of box body (1), wherein, the volume of left accommodation space is 1/3 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates (3), 96 hole microwell plates (3) are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates (3) is provided with immobilised swine foot-and-mouth disease virus envelope antigen, described right accommodation space is provided with one with the reagent bottle pedestal (4) of a plurality of recessed bottles position, in reagent bottle pedestal (4), be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.
2. a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit as claimed in claim 1, is characterized in that: on the box wall of the left accommodation space of described box body (1), be coated with one deck froth bed.
3. a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit as claimed in claim 2, it is characterized in that: described division board (2) is plastic material, the inwall correspondence of box body (1) is provided with two grooves from plate (2), and division board (2) is plugged in groove.
4. a kind of swine foot-and-mouth disease virus antibody chemical luminescence kit as claimed in claim 3, is characterized in that: the thickness of described division board (2) is 2 times of box body (1) thickness, and the thickness of the upper froth bed of division board (2) is 2 times of division board (2) thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320699111.7U CN203825009U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320699111.7U CN203825009U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203825009U true CN203825009U (en) | 2014-09-10 |
Family
ID=51480492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320699111.7U Expired - Lifetime CN203825009U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203825009U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105759058A (en) * | 2016-04-26 | 2016-07-13 | 中国科学院微生物研究所 | Method and special kit for detecting O-type foot-and-mouth disease virus antibody of pigs |
| CN106370854A (en) * | 2016-08-17 | 2017-02-01 | 中国科学院微生物研究所 | Duck hepatitis A virus 1 antibody detection method and special-purpose kit thereof |
| CN106596932A (en) * | 2016-11-25 | 2017-04-26 | 中国农业科学院兰州兽医研究所 | Chemiluminescence detection kit of swine foot-and-mouth disease 3ABC and 2C antibodies |
-
2013
- 2013-11-08 CN CN201320699111.7U patent/CN203825009U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105759058A (en) * | 2016-04-26 | 2016-07-13 | 中国科学院微生物研究所 | Method and special kit for detecting O-type foot-and-mouth disease virus antibody of pigs |
| CN106370854A (en) * | 2016-08-17 | 2017-02-01 | 中国科学院微生物研究所 | Duck hepatitis A virus 1 antibody detection method and special-purpose kit thereof |
| CN106596932A (en) * | 2016-11-25 | 2017-04-26 | 中国农业科学院兰州兽医研究所 | Chemiluminescence detection kit of swine foot-and-mouth disease 3ABC and 2C antibodies |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yan Ruoqian Inventor after: Li Xiumei Inventor after: Fang Xianzhen Inventor after: Liu Yubao Inventor after: Xie Caihua Inventor after: Zhao Xueli Inventor before: Wang Shanpu Inventor before: Li Xiumei Inventor before: Liu Huan Inventor before: Wang Qingli Inventor before: Huang Xi |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WANG SHANPU LI XIUMEI LIU HUAN WANG QINGLI HUANG XI TO: YAN RUOQIAN LI XIUMEI FANG XIANZHEN LIU YUBAO XIE CAIHUA ZHAO XUELI |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Chemiluminescence reagent kit of porcine foot-mouth disease virus antibody Effective date of registration: 20191017 Granted publication date: 20140910 Pledgee: Zhengzhou SME Company limited by guarantee Pledgor: LUOYANG LAIPSON INFORMATION TECHNOLOGY Co.,Ltd. Registration number: Y2019410000008 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140910 |
|
| CX01 | Expiry of patent term |