CN202929038U - Levodopa chemiluminescence detection kit - Google Patents
Levodopa chemiluminescence detection kit Download PDFInfo
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- CN202929038U CN202929038U CN 201220583955 CN201220583955U CN202929038U CN 202929038 U CN202929038 U CN 202929038U CN 201220583955 CN201220583955 CN 201220583955 CN 201220583955 U CN201220583955 U CN 201220583955U CN 202929038 U CN202929038 U CN 202929038U
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- levodopa
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Abstract
The utility model relates to a levodopa content chemiluminescence kit. The kit comprises a microwell plate, an enzyme-labelled antibody working solution, seven standard product solutions with different concentrations, a substrate luminescent solution A, a substrate luminescent solution B and a solid phosphoric acid-Tween buffer solution. A coupling antigen is pre-coated on a microwell strip of the microwell plate by adopting a chemoluminescence method, levodopa contained in a sample and the coupling antigen pre-coated on the microwell strip compete for an enzyme-labelled levodopa antibody, the substrate luminescent solutions are used for luminescence, and the content of a levodopa medicament in the sample can be obtained by utilizing the inverse relationship between a luminescence value and the content of the levodopa. The levodopa content chemiluminescence kit disclosed by the utility model has the characteristics of high speed, high sensitivity and the like, and can play an important role in detecting the content of the levodopa in the biological sample.
Description
Technical field:
The utility model relates to a kind of chemoluminescence method detection technique field of drug test, is specifically related to levodopa chemoluminescence method detection technique field.
Technical background:
Levodopa has another name called levodopa (L-dopa), is antiparkinsonian drug.Enter brain tissue by blood-brain barrier, play a role.Be applicable to primary parkinsonism and non-medicine originality Parkinsonism.The bad reactions such as nauseating, vomiting, poor appetite and insomnia, illusion after taking, this product are arranged.But the toxic and side effect of levodopa is larger, and the treatment window is little.Must the interior levodopa concentration of regular human body.
In long-term regularly human body, levodopa concentration is a clinical conventional sense project.Existing detection method has high-efficient liquid phase technique and vapor-phase chromatography, but has inconvenient and the low characteristics of sensitivity of detecting.
Summary of the invention:
The purpose of this utility model is a kind of levodopa chemoluminescence method detection kit of design, the content of levodopa medicine in detection of biological sample that can be quick, sensitive.
The utility model is a kind of levodopa chemoluminescence method detection kit, comprise 96 hole microwell plates, 10 bottles of reagent in box body and box, 1 bag of solid phosphoric acid-tween damping fluid, 96 hole microwell plates are solid carrier, pre-coated coupling levodopa antigen, 10 bottles of reagent are respectively 7 bottles of standard solutions, 1 bottle of enzyme labelled antibody working fluid, 1 bottle of substrate luminescent solution A, 1 bottle of substrate luminescent solution B.Box body is carton box, and 96 hole microwell plates are placed in the vacuum Fresco Bag, and solid phosphoric acid-tween damping fluid is placed in sealing bag, 7 bottles of standard solutions, and 1 bottle of enzyme labelled antibody working fluid, 1 bottle of substrate luminescent solution A cover PE translucent plastic bottle with deceiving.1 bottle of substrate luminescent solution B covers PE black plastic bottle with deceiving.Microwell plate is comprised of outer gimbal support and dismountable 8 the enzyme mark bars that are placed on it, each dismountable enzyme mark bar has 12 micropores, 7 bottles of standard solutions, 1ml/ bottle, 1 bottle of enzyme labelled antibody working fluid, 10ml, each 1 bottle of substrate luminescent solution A, substrate luminescent solution B, 10ml/ bottle, 1 bottle of stop buffer, 10ml, solid phosphoric acid-1 bag of tween damping fluid, 10g.
The utility model principle is the chemoluminescence method method, pre-coated coupled antigen on microwell plate enzyme mark bar, the levodopa that contains in sample will be competed the anti-levodopa antibody of enzyme target with pre-coated coupled antigen on enzyme mark bar, luminous with the substrate luminescent solution, the sample luminous value becomes negative correlation with the content of its contained levodopa, compare with typical curve, then multiply by its corresponding extension rate, can draw the content of levodopa medicine in sample.
Beneficial effect:
The beneficial effects of the utility model be can be quick, sensitive the detection of biological sample in the content of levodopa medicine.
Description of drawings:
Fig. 1 is the utility model box body schematic diagram, and wherein 1 is box body;
Fig. 2 is the schematic diagram of the utility model 96 hole microwell plates, and wherein 2 is 96 hole microwell plates, and 3 is pre-coated coupling levodopa antigen micropore, and 4 is the outer gimbal support of immobilized enzyme mark bar;
Fig. 3 is the schematic diagram of the utility model solid phosphoric acid-tween damping fluid, and wherein 5 is packed solid phosphoric acid-tween damping fluid;
Fig. 4 is the utility model reagent bottle schematic diagram, and wherein 6 is standard solution, and 7 is enzyme labelled antibody working fluid, substrate luminescent solution A; 8 is substrate luminescent solution B.
Embodiment:
Embodiment 1: a kind of levodopa chemoluminescence method detection kit, 96 hole microwell plates 2,10 bottles of reagent in box body 1 and box, 1 bag of solid phosphoric acid-tween damping fluid 5,96 hole microwell plates are solid carrier, pre-coated coupling levodopa antigen micropore 3,10 bottles of reagent are respectively 6,1 bottles of enzyme labelled antibody working fluids 7 of 7 bottles of standard solutions, 1 bottle of substrate luminescent solution A7,1 bottle of substrate luminescent solution B8.Box body is carton box, and 96 hole microwell plates are placed in the vacuum Fresco Bag, and 6,1 bottles of enzyme labelled antibody working fluids 7 of 7 bottles of standard solutions, 1 bottle of substrate luminescent solution A7 cover PE translucent plastic bottle with deceiving.1 bottle of substrate luminescent solution B8 covers PE black plastic bottle with deceiving.Microwell plate is comprised of outer gimbal support 4 and dismountable 8 the enzyme mark bars that are placed on it, each dismountable enzyme mark bar has 12 micropores, 7 bottles of standard solutions, 1ml/ bottle, 1 bottle of enzyme labelled antibody working fluid, 10ml, each 1 bottle of substrate luminescent solution A, substrate luminescent solution B, 10ml/ bottle, 1 bottle of stop buffer, 10ml, solid phosphoric acid-1 bag of tween damping fluid, 10g.
Human plasma is added in the microwell plate of pre-coated coupling levodopa antigen by 50 μ l/ holes, add simultaneously enzyme labelled antibody working fluid 50 μ l/ holes, 37 ℃, hatch 1h, with cleansing solution washing 3 times, then add substrate luminescent solution 50 μ l/ holes, room temperature 3-5min, Chemiluminescence Apparatus detects, and namely gets levodopa concentration.
Claims (2)
1. levodopa chemoluminescence method detection kit, it is characterized in that: comprise 96 hole microwell plates, 10 bottles of reagent in box body and box, 1 bag of solid phosphoric acid-tween damping fluid is provided with pre-coated coupling levodopa antigen micropore in wherein said 96 hole microwell plates; 10 bottles of reagent are respectively 7 bottles of standard solutions, 1 bottle of enzyme labelled antibody working fluid, 1 bottle of substrate luminescent solution A, 1 bottle of substrate luminescent solution B.
2. a kind of levodopa chemoluminescence method detection kit according to claim 1, it is characterized in that: described 96 hole microwell plates are comprised of outer gimbal support and dismountable 8 the enzyme mark bars that are placed on it, each dismountable enzyme mark bar has 12 micropores, 7 bottles of standard solutions, 1ml/ bottle, 1 bottle of enzyme labelled antibody working fluid, 10ml, each 1 bottle of substrate luminescent solution A, substrate luminescent solution B, 10ml/ bottle, solid phosphoric acid-1 bag of tween damping fluid, 10g.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220583955 CN202929038U (en) | 2012-11-08 | 2012-11-08 | Levodopa chemiluminescence detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220583955 CN202929038U (en) | 2012-11-08 | 2012-11-08 | Levodopa chemiluminescence detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202929038U true CN202929038U (en) | 2013-05-08 |
Family
ID=48218987
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220583955 Expired - Lifetime CN202929038U (en) | 2012-11-08 | 2012-11-08 | Levodopa chemiluminescence detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202929038U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103412118A (en) * | 2013-08-13 | 2013-11-27 | 厦门市云鹏科技发展有限公司 | Shading ELISA plate with transparent bottom and shading ELISA plate apparatus |
-
2012
- 2012-11-08 CN CN 201220583955 patent/CN202929038U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103412118A (en) * | 2013-08-13 | 2013-11-27 | 厦门市云鹏科技发展有限公司 | Shading ELISA plate with transparent bottom and shading ELISA plate apparatus |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130508 |