CN202886380U - Diazepam ELISA (enzyme linked immunosorbent assay) kit - Google Patents
Diazepam ELISA (enzyme linked immunosorbent assay) kit Download PDFInfo
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- CN202886380U CN202886380U CN 201220583986 CN201220583986U CN202886380U CN 202886380 U CN202886380 U CN 202886380U CN 201220583986 CN201220583986 CN 201220583986 CN 201220583986 U CN201220583986 U CN 201220583986U CN 202886380 U CN202886380 U CN 202886380U
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- diazepam
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Abstract
The utility model relates to an ELISA (enzyme linked immunosorbent assay) kit for detecting diazepam content in blood, serum, plasma and urine of a human body. The kit comprises an ELISA plate, an enzyme labeled secondary antibody working solution, an antibody working solution, standard solutions with 7 concentrations, a substrate color development solution, a stop solution and a washing solution. According to indirect competitive ELISA method, a coupled antigen is precoated on the microporous strip of the ELISA plate, diazepam contained in a sample and the coupled antigen precoated on the microporous strip compete a diazepam antibody; the substrate color development solution develops color after adding the enzyme labeled secondary antibody; and the content of diazepam in the sample can be obtained according to the inverse relationship between the sample absorbance and the content of diazepam in the sample. The ELISA kit provided by the utility model has the characteristics of high speed, high sensitivity and the like, and plays an important role in detecting diazepam content in biological samples.
Description
Technical field:
The utility model relates to a kind of ELISA detection technique field of drug test, is specifically related to diazepam ELISA detection technique field.
Technical background:
Diazepam has another name called stablely, and diazepam has antianxiety, calmness, hypnosis, anticonvulsion, anti-epileptic and central myorelaxant effects.Clinical practice is in treatment: anxiety disorder and various neurosis; Insomnia, especially splendid to anxiety aypnia curative effect; Epilepsy can be share with other antiepileptic, and treatment grand mal or petit mal are answered intravenous injection during the control status epilepticus; The convulsions that a variety of causes causes is such as eclampsia, lockjaw, children's's high fever convulsions etc.; Cerebrovas-cularaccident or spinal cord injury sexual centre flesh by force should or lumbar muscle strain, endoscopy etc. due to muscle cramp.But the toxic and side effect of diazepam is larger, and the treatment window is little.Must the interior diazepam concentration of regular human body.
Diazepam concentration is a clinical conventional sense project in the long-term regularly human body.Existing test item has high-efficient liquid phase technique and vapor-phase chromatography, but has inconvenient and the low characteristics of sensitivity of detecting.
Summary of the invention:
The purpose of this utility model is a kind of diazepam ELISA detection kit of design, the content of diazepam medicine in the detection of biological sample that can be quick, sensitive.
The utility model is a kind of diazepam ELISA detection kit, comprise 96 hole ELISA Plate, 12 bottles of reagent in box body and the box, 96 hole ELISA Plate are solid carrier, pre-coated coupling diazepam antigen, 12 bottles of reagent, be respectively 7 bottles of standard solutions, 1 bottle of ELIAS secondary antibody working fluid, 1 bottle of antibody working fluid, 1 bottle of substrate nitrite ion, 1 bottle of stop buffer, 1 bottle of cleansing solution.Box body is carton box, and 96 hole ELISA Plate are placed in the vacuum Fresco Bag, 7 bottles of standard solutions, and 1 bottle of ELIAS secondary antibody working fluid, 1 bottle of antibody working fluid, 1 bottle of stop buffer, 1 bottle of cleansing solution cover PE translucent plastic bottle with deceiving.1 bottle of substrate nitrite ion covers PE black plastic bottle with deceiving.ELISA Plate is comprised of outer gimbal support and dismountable 12 the enzyme mark bars that are placed on it, and each dismountable enzyme mark bar has 8 micropores, 7 bottles of standard solutions, the 1ml/ bottle, 1 bottle of ELIAS secondary antibody working fluid, 10ml, 1 bottle of antibody working fluid, 10ml, 1 bottle of substrate nitrite ion, 12ml, 1 bottle of stop buffer, 10ml, 1 bottle of cleansing solution, 500ml.
The utility model principle is to adopt the indirect competitive ELISA method, pre-coated coupled antigen on the ELISA Plate capillary strip, the diazepam that contains in the sample will be competed anti-diazepam antibody with pre-coated coupled antigen on the capillary strip, after adding ELIAS secondary antibody, with the colour developing of substrate nitrite ion, the sample light absorption value becomes negative correlation with the content of its contained diazepam, compares with typical curve, multiply by again its corresponding extension rate, can draw the content of diazepam medicine in the sample.
Beneficial effect:
The beneficial effects of the utility model be can be quick, sensitive the detection of biological sample in the content of diazepam medicine.
Description of drawings:
Fig. 1 is the utility model box body synoptic diagram, and wherein 1 is box body;
Fig. 2 is the synoptic diagram of the utility model 96 hole ELISA Plate, and wherein 2 is 96 hole ELISA Plate, and 3 is pre-coated coupling diazepam antigen enzyme mark hole, and 4 is the outer gimbal support of immobilized enzyme mark bar;
Fig. 3 is the utility model reagent bottle synoptic diagram, and wherein 5 for encapsulating the black lid PE translucent plastic bottles of 1ml standard solution, the 6 black lid PE translucent plastic bottles for encapsulation 10ml ELIAS secondary antibody working fluid, antibody working fluid, stop buffer; The 7 black lid PE black plastic bottles for encapsulation 10m substrate nitrite ion; The 8 black lid PE translucent plastic bottles for encapsulation 500ml cleansing solution.
Embodiment:
Embodiment 1: a kind of diazepam ELISA detection kit, 96 hole ELISA Plate 2,12 bottles of reagent in box body 1 and the box, 96 hole ELISA Plate 2 are solid carrier, in be provided with pre-coated coupling diazepam antigen enzyme mark hole 3,12 bottles of reagent, be respectively 7 bottles of standard solutions, 1 bottle of ELIAS secondary antibody working fluid, 1 bottle of antibody working fluid, 1 bottle of substrate nitrite ion, 1 bottle of stop buffer, 1 bottle of cleansing solution.Box body is carton box, 96 hole ELISA Plate 2 are placed in the vacuum Fresco Bag, 7 bottles of standard solutions, 1 bottle of ELIAS secondary antibody working fluid are packaged in black 6, the 1 bottle of antibody working fluid of PE translucent plastic bottle that cover and are packaged in black 6, the 1 bottle of stop buffer of PE translucent plastic bottle that cover and are packaged in to deceive and cover 6,1 bottle of cleansing solution of PE translucent plastic bottle and be packaged in to deceive and cover PE translucent plastic bottle 8.1 bottle of substrate nitrite ion is packaged in the black PE black plastic bottle 7 that covers.96 hole ELISA Plate 2 are comprised of outer gimbal support 4 and dismountable 12 the enzyme mark bars that are placed on it, and each dismountable enzyme mark bar has 8 micropores (namely marking hole 3), 7 bottles of standard solutions, the 1ml/ bottle, 1 bottle of ELIAS secondary antibody working fluid, 10ml, 1 bottle of antibody working fluid, 10ml, 1 bottle of substrate nitrite ion, 12ml, 1 bottle of stop buffer, 10ml, 1 bottle of cleansing solution, 500ml.
Human plasma is added in the ELISA Plate of pre-coated coupling diazepam antigen by 50 μ l/ holes, add simultaneously antibody working fluid 50 μ l/ holes, 37 ℃, hatch 1h, with cleansing solution washing 3 times, add ELIAS secondary antibody working fluid 50 μ l/ holes, 37 ℃, hatch 1h, with cleansing solution washing 3 times, add again substrate nitrite ion 50 μ l/ holes, room temperature 10min, add stop buffer 50 μ l/ holes and stop, microplate reader detects, and namely gets diazepam concentration.
Claims (2)
1. diazepam ELISA detection kit, comprise 96 hole ELISA Plate, 12 bottles of reagent in box body and the box, it is characterized in that: be provided with pre-coated coupling diazepam antigen enzyme mark hole in the described 96 hole ELISA Plate, 12 bottles of reagent, be respectively 7 bottles of standard solutions, 1 bottle of ELIAS secondary antibody working fluid, 1 bottle of antibody working fluid, 1 bottle of substrate nitrite ion, 1 bottle of stop buffer, 1 bottle of cleansing solution.
2. according to claims 1 described a kind of diazepam ELISA detection kit, it is characterized in that: described 96 hole ELISA Plate are comprised of outer gimbal support and dismountable 12 the enzyme mark bars that are placed on it, and each dismountable enzyme mark bar has 8 micropores, 7 bottles of standard solutions, 1ml/ bottle, 1 bottle of ELIAS secondary antibody working fluid, 10ml, 1 bottle of antibody working fluid, 10ml, 1 bottle of substrate nitrite ion, 12ml, 1 bottle of stop buffer, 10ml, 1 bottle of cleansing solution, 500ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220583986 CN202886380U (en) | 2012-11-08 | 2012-11-08 | Diazepam ELISA (enzyme linked immunosorbent assay) kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220583986 CN202886380U (en) | 2012-11-08 | 2012-11-08 | Diazepam ELISA (enzyme linked immunosorbent assay) kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202886380U true CN202886380U (en) | 2013-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220583986 Expired - Lifetime CN202886380U (en) | 2012-11-08 | 2012-11-08 | Diazepam ELISA (enzyme linked immunosorbent assay) kit |
Country Status (1)
| Country | Link |
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| CN (1) | CN202886380U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645764A (en) * | 2016-12-26 | 2017-05-10 | 北京勤邦生物技术有限公司 | Enzyme-linked immunosorbent assay kit for detecting diazepam and application thereof |
-
2012
- 2012-11-08 CN CN 201220583986 patent/CN202886380U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645764A (en) * | 2016-12-26 | 2017-05-10 | 北京勤邦生物技术有限公司 | Enzyme-linked immunosorbent assay kit for detecting diazepam and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130417 |