CN203825011U - Chemiluminescence kit for swine pseudorabies virus antibody - Google Patents
Chemiluminescence kit for swine pseudorabies virus antibody Download PDFInfo
- Publication number
- CN203825011U CN203825011U CN201320699106.6U CN201320699106U CN203825011U CN 203825011 U CN203825011 U CN 203825011U CN 201320699106 U CN201320699106 U CN 201320699106U CN 203825011 U CN203825011 U CN 203825011U
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- Prior art keywords
- bottle
- box body
- division board
- kit
- accommodation space
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- Expired - Lifetime
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- 241000282898 Sus scrofa Species 0.000 title abstract description 10
- 241000701093 Suid alphaherpesvirus 1 Species 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 15
- 239000000427 antigen Substances 0.000 claims abstract description 11
- 102000036639 antigens Human genes 0.000 claims abstract description 11
- 108091007433 antigens Proteins 0.000 claims abstract description 11
- 239000000126 substance Substances 0.000 claims abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 239000012530 fluid Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000872 buffer Substances 0.000 claims abstract description 7
- 108010001336 Horseradish Peroxidase Proteins 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims abstract description 4
- 230000004308 accommodation Effects 0.000 claims description 18
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 13
- 238000004020 luminiscence type Methods 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 150000003016 phosphoric acids Chemical class 0.000 claims description 3
- 239000012086 standard solution Substances 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 238000001514 detection method Methods 0.000 abstract description 4
- 238000004458 analytical method Methods 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 210000002966 serum Anatomy 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract 4
- 241000283707 Capra Species 0.000 abstract 1
- 241000282887 Suidae Species 0.000 abstract 1
- 108010003533 Viral Envelope Proteins Proteins 0.000 abstract 1
- 239000008055 phosphate buffer solution Substances 0.000 abstract 1
- 238000004445 quantitative analysis Methods 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 239000007790 solid phase Substances 0.000 description 3
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000002381 plasma Anatomy 0.000 description 2
- 238000012113 quantitative test Methods 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to a chemiluminescence kit for swine pseudorabies virus antibody. The chemiluminescence kit comprises a kit body, wherein the left containing space of the kit body is internally provided with a 96-pore micropore plate; a plate sealing film used for shading is wrapped outside the 96-pore micropore plate; the bottom of the plate pore surface of the 96-pore micropore plate is provided with swine pseudorabies virus envelope antigen; the right containing space of the kit body is provided with a reagent bottle base provided with a plurality of lower concave bottle positions; the reagent bottle base is internally provided with a bottle of goat anti-pig antibody working fluid marked by horseradish peroxidase, a bottle of concentrated phosphate buffer solution, a bottle of concentrated washing liquid, a bottle of luminous substrate liquid A, a bottle of luminous substrate liquid B, six bottles of standard substance solution, a bottle of stop buffer and two bottles of yin and yang comparison working fluid. The chemiluminescence kit has the characteristics of high detection sensitivity and flexible application, is capable of realizing full-automatic analysis, can be used for carrying out quantitative analysis on the swine pseudorabies virus antibody in serum or plasma of pigs, and is beneficial to guiding the immune time, thus providing guarantee for farmers.
Description
Technical field
The utility model relates to field of immunodetection, specifically relates to a kind of PRV antibody chemical luminescence kit.
Background technology
At present, swine foot-and-mouth disease virus detects mainly taking aetology and detects and serology detects as main, and enzyme linked immunosorbent assay, fluorescence labeling are tested, fluorescence RT-PCR is for detection of the antibody of swine foot-and-mouth disease virus.
(1) enzyme linked immunosorbent assay
Utilize antigen and antibody capable to be combined with surface of solid phase carriers and can keep its immunity,
Label after antigen or antibody and enzyme conjugates can keep the immunocompetence of antigen or antibody, can keep again the activity of enzyme, the antibody of the antigen in examined object or antibody and solid phase surface or antigen generation immune response are also combined in solid phase surface, this antigen antibody complex again can be in conjunction with corresponding enzyme labeling thing, unconjugated enzyme labeling thing is removed in washing, add enzyme reaction substrate, the process that substrate is coloured product by enzymatic.Shortcoming: length consuming time, complex operation step, cross pollution easily occurs.
(2) fluorescence labeling test
After utilizing fluorchrome to be combined with label, do not affect the immunocompetence of label, can be comparatively firm with specific antigen in sample or antibody specific binding and this combination, while washing with damping fluid, remove after free fluorescein-labelled label, by the immunology tracer technique of fluorescence microscope.Shortcoming: equipment manufacturing cost is higher, diagnosticum is difficult to buy, and is difficult to now popularization and application.Meanwhile, be subject to slide thickness and observing time and observer's experience, the restriction of skill level.
(3) fluorescence RC-PCR
Fluorescence RC-PCR is the new technology growing up late 1990s, and fluorescein-labeled probe is reacted together with primer in luminoscope, and computer carries out Real-Time Monitoring to whole reaction.Shortcoming: operating personnel should wear masks, often changes disposable glove.
Highly sensitive is the superiority of chemiluminescence immune assay key, and easy and simple to handle.Chemiluminescence immune assay can detect the material that the method such as radiommunoassay and enzyme-linked immuno assay cannot detect.
Utility model content
The purpose of this utility model is the deficiency for solving the problems of the technologies described above, and provides and plants one by one PRV antibody chemical luminescence kit, highly sensitive, sensing range is wide, easy and simple to handle fast and nontoxic pollution-free.
The utility model is the deficiency solving the problems of the technologies described above, the technical scheme adopting is: a kind of PRV antibody chemical luminescence kit, comprise box body, box body is the papery box body with upper cover, described box body is divided into a left side by division board, right two accommodation spaces, division board is perpendicular to the base plate setting of box body, wherein, the volume of left accommodation space is 1/4 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates, 96 hole microwell plates are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates is provided with immobilised PRV envelope antigen, described right accommodation space is provided with a reagent bottle pedestal with multiple recessed bottles position, in reagent bottle pedestal, be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.
On one side sidewall of described reagent bottle pedestal, offer two rectangular recess that be arranged in parallel.
On the box wall of the left accommodation space of described box body, be coated with one deck froth bed.
Described division board is plastic material, and the inwall correspondence of box body is provided with two grooves from plate, and division board is plugged in groove.
The thickness of described division board is 2 times of box body thickness, and on division board, the thickness of froth bed is 2 times of division board thickness.
beneficial effect
Kit of the present utility model has that detection sensitivity is high, applying flexible, can carry out the feature of automatical analysis, can carry out quantitative test to PRV antibody in pig serum or blood plasma, is beneficial to the immunity opportunity instructed, for raiser provides safeguard.
Brief description of the drawings
Fig. 1 is the inner structure schematic diagram after the utility model lid is opened;
Fig. 2 is the structural representation of the utility model reagent bottle pedestal;
Mark in figure: 1, box body, 2, upper cover, 3,96 hole microwell plates, 4, reagent bottle pedestal 5, rectangular recess, 6, froth bed.
Embodiment
As shown in the figure: a kind of PRV antibody chemical luminescence kit, comprise box body 1, box body 1 is the papery box body with upper cover 2, described box body 1 is divided into a left side by division board 2, right two accommodation spaces, division board 2 is perpendicular to the base plate setting of box body 1, wherein, the volume of left accommodation space is 1/3 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates 3, 96 hole microwell plates 3 are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates 3 is provided with immobilised PRV envelope antigen, described right accommodation space is provided with a reagent bottle pedestal 4 with multiple recessed bottles position, on one side sidewall of described reagent bottle pedestal 4, offer two rectangular recess that be arranged in parallel 5, rectangular recess 5 is convenient for people in use reagent bottle pedestal 4 be plucked out, in reagent bottle pedestal 4, be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.On the box wall of the left accommodation space of described box body 1, be coated with one deck froth bed 6.Described division board 2 is plastic material, and the inwall correspondence of box body 1 is provided with two grooves from plate 2, and division board 2 is plugged in groove.The thickness of described division board 2 is 2 times of box body 1 thickness, and on division board 2, the thickness of froth bed is 2 times of division board 2 thickness.Kit of the present utility model has that detection sensitivity is high, applying flexible, can carry out the feature of automatical analysis, can carry out quantitative test to PRV antibody in pig serum or blood plasma, be beneficial to the immune opportunity of instructing, for raiser provides safeguard, the reasonable in design of box body in addition, damage easy to use, effectively to avoid kit in transport, disassembly process, reagent bottle to be caused.
Claims (4)
1. a PRV antibody chemical luminescence kit, comprise box body (1), box body (1) is the papery box body with upper cover (2), it is characterized in that: described box body (1) is divided into a left side by division board (2), right two accommodation spaces, division board (2) is perpendicular to the base plate setting of box body (1), wherein, the volume of left accommodation space is 1/4 of right accommodation space volume, in described left accommodation space, be provided with 96 hole microwell plates (3), 96 hole microwell plates (3) are wrapped with the shrouding film for shading, the bottom, plate hole surface of 96 hole microwell plates (3) is provided with immobilised PRV envelope antigen, described right accommodation space is provided with a reagent bottle pedestal (4) with multiple recessed bottles position, on one side sidewall of described reagent bottle pedestal (4), offer two rectangular recess that be arranged in parallel (5), in reagent bottle pedestal (4), be provided with the antibody working fluid of the goat-anti pig of 1 bottle of horseradish peroxidase mark, 1 bottle of concentrated phosphoric acid salt buffer, 1 bottle of concentrated cleaning solution, 1 bottle of luminous substrate liquid A, 1 bottle of luminous substrate liquid B, 6 bottles of standard solutions, 1 bottle of stop buffer and 2 bottles of negative and positive contrast working fluids.
2. a kind of PRV antibody chemical luminescence kit as claimed in claim 1, is characterized in that: on the box wall of the left accommodation space of described box body (1), be coated with one deck froth bed (6).
3. a kind of PRV antibody chemical luminescence kit as claimed in claim 2, it is characterized in that: described division board (2) is plastic material, the inwall correspondence of box body (1) is provided with two grooves from plate (2), and division board (2) is plugged in groove.
4. a kind of PRV antibody chemical luminescence kit as claimed in claim 3, is characterized in that: the thickness of described division board (2) is 2 times of box body (1) thickness, and the thickness of the upper froth bed of division board (2) is 2 times of division board (2) thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320699106.6U CN203825011U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence kit for swine pseudorabies virus antibody |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320699106.6U CN203825011U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence kit for swine pseudorabies virus antibody |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203825011U true CN203825011U (en) | 2014-09-10 |
Family
ID=51480494
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320699106.6U Expired - Lifetime CN203825011U (en) | 2013-11-08 | 2013-11-08 | Chemiluminescence kit for swine pseudorabies virus antibody |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203825011U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109444410A (en) * | 2018-10-26 | 2019-03-08 | 成都普利泰生物科技有限公司 | A kind of veterinary rabies poison neutralizing antibody chemiluminescence detection kit |
-
2013
- 2013-11-08 CN CN201320699106.6U patent/CN203825011U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109444410A (en) * | 2018-10-26 | 2019-03-08 | 成都普利泰生物科技有限公司 | A kind of veterinary rabies poison neutralizing antibody chemiluminescence detection kit |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wang Shanpu Inventor after: Yan Ruoqian Inventor after: Fang Xianzhen Inventor after: Li Xiumei Inventor after: Liu Meifang Inventor after: Zhang Pan Inventor after: Xie Caihua Inventor before: Wang Shanpu Inventor before: Jin Wanxia Inventor before: Wang Xiaojun Inventor before: Hu Xiuqing Inventor before: Zhang Jianghong |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WANG SHANPU JIN WANXIA WANG XIAOJUN HU XIUQING ZHANG JIANGHONG TO: WANG SHANPU YAN RUOQIAN FANG XIANZHEN LI XIUMEI LIU MEIFANG ZHANG PAN XIE CAIHUA |
|
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Xiumei Inventor after: Yan Ruoqian Inventor after: Fang Xianzhen Inventor after: Liu Meifang Inventor after: Zhang Panpan Inventor after: Xie Caihua Inventor after: Liu Yubao Inventor after: Li Xiao Inventor after: Li Quanwei Inventor before: Wang Shanpu Inventor before: Yan Ruoqian Inventor before: Fang Xianzhen Inventor before: Li Xiumei Inventor before: Liu Meifang Inventor before: Zhang Pan Inventor before: Xie Caihua |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WANG SHANPU YAN RUOQIAN FANG XIANZHEN LI XIUMEI LIU MEIFANG ZHANG PAN XIE CAIHUA TO: LI XIUMEI YAN RUOQIAN FANG XIANZHEN LIU MEIFANG ZHANG PANPAN XIE CAIHUA LIU YUBAO LI XIAO LI QUANWEI |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Chemiluminescence kit for swine pseudorabies virus antibody Effective date of registration: 20191017 Granted publication date: 20140910 Pledgee: Zhengzhou SME Company limited by guarantee Pledgor: LUOYANG LAIPSON INFORMATION TECHNOLOGY Co.,Ltd. Registration number: Y2019410000008 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140910 |
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| CX01 | Expiry of patent term |