CN204495830U - Novel immune trapped enzyme urgees luminescence detection kit - Google Patents
Novel immune trapped enzyme urgees luminescence detection kit Download PDFInfo
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- CN204495830U CN204495830U CN201520194714.0U CN201520194714U CN204495830U CN 204495830 U CN204495830 U CN 204495830U CN 201520194714 U CN201520194714 U CN 201520194714U CN 204495830 U CN204495830 U CN 204495830U
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Abstract
The utility model discloses a kind of Novel immune trapped enzyme and urge luminescence detection kit, described kit comprise pre-coated the immunocapture rod, antigen containing enzyme labeling or the antibody-solutions that have antigen or antibody the first reaction cup, the second reaction cup containing wash solution, strengthen liquid and H containing luminous substrate
2o
2the 3rd reaction cup, described immunocapture rod can be planted respectively and be active in first, second and third reaction cup described, and first, second and third reaction cup described is arranged independently of one another.Described kit also can include the box body of a upper opening, and first, second and third reaction cup described is all located in box body.Compared to existing technology, chemiluminescence immunoassay can obtain applying widely more flexibly by this patent.This patent simplifies experimental procedure, shortens the time of experiment, can reduce experimental cost widely simultaneously.
Description
Technical field
The utility model relates to a kind of Novel immune trapped enzyme and urgees luminescence detection kit.
Background technology
The method that traditional enzymatic electrochemiluminescent immunoassay detection kit uses comes labelled antigen or antibody with the enzyme (as horseradish peroxidase or alkaline phosphatase) participating in a certain chemiluminescence reaction of catalysis, after there is immune response in the antibody (antigen) corresponding to sample to be measured, form the immune complex of solid-phase coating antigen or antibody-test antibodies or antigen-form such as enzyme-labelled antigen or antibody, after washing, add substrate (luminous agent), enzymatic Sum decomposition substrate is luminous, by detecting a kind of method of the content of antibody (antigen) in sample to be measured to the luminous intensity of substrate.The General Principle step of experiment is: 1. react in micropore by pre-coated for reaction micropore the inside surface 2. antigen of sample to be tested and enzyme labeling or antibody can being added to the antigen of measured matter generation specific reaction or antibody, insulation reaction a period of time, make it form antigenantibody complex 3. reaction micropore to be placed on specific wash mill or instrument and repeatedly to rinse, thorough eccysis free in conjunction with material and chaff interference, reinforcing agent and luminous substrate (as luminol) are 4. added by the antigenantibody complex of only remaining band enzyme labeling in reaction micropore again to react in micropore, at H
2o
2liquid environment under, insulation reaction a period of time, enzymatic Sum decomposition substrate is luminous, according to the monitoring to luminous intensity, can calculate the concentration of the analysis thing of sample.Washing process is related in the experimental procedure of tradition enzymatic electrochemiluminescent immunoassay detection kit, because course of reaction all completes in same reaction micropore, cleaning in reaction micropore proposes strict requirement for the equipment washed or device, add laboratory operating procedures simultaneously, must coordinate under the special instrument of complex and expensive so cause enzymatic lighting immunity analysis to be tested, limit the condition of testing and carrying out.
What wash conditions restriction brought is necessary for the supporting expensive device of experiment, and current kit laboratory operating procedures is loaded down with trivial details.
Utility model content
In view of this, technical problem to be solved in the utility model is: provide a kind of operation steps Novel immune trapped enzyme that is more simple and saving experimental cost that can make to urge luminescence detection kit.
The know-why of this patent is on the basis of the pre-coated solid phase carrier changing antigen or antibody in traditional enzymatic electrochemiluminescent immunoassay detection kit, change traditional experiment thinking and step, can with the antigen of measured matter generation specific reaction or antibody pre-coated on immunocapture rod, because pre-coated immunocapture rod can move freely in the liquid of different vessels, immunocapture rod can be detached solution after the immune complex of trapped enzyme mark, then necessary cleaning (such as soaking in washing lotion) is carried out to the barred body capturing enzyme labeled immunoassay compound, remove the free non-bond or other interfering materials that are attached to surface, finally immunocapture rod is immersed in substrate solution, enzymatic Sum decomposition substrate is luminous, carry out luminous intensity monitoring, read result.
In order to achieve the above object, the utility model adopts following technical scheme to realize:
A kind of Novel immune trapped enzyme urgees luminescence detection kit, and described kit comprises pre-coatedly has the first reaction cup of the immunocapture rod of antigen or antibody, the antigen containing enzyme labeling or antibody-solutions, the second reaction cup containing wash solution, strengthen liquid and H containing luminous substrate
2o
2the 3rd reaction cup, described immunocapture rod can be planted respectively and be active in first, second and third reaction cup described, and first, second and third reaction cup described is arranged independently of one another.
As preferably, described kit also includes the box body of a upper opening, and first, second and third reaction cup described is all located in box body.
As shown from the above technical solution, the beneficial effects of the utility model are:
Compared to existing technology, this patent to show be exactly to solve described in current enzymatic electrochemiluminescent immunoassay kit in experiment because harsh washing requires the experiment condition restricted problem of complex apparatus or the matched with devices brought, chemiluminescence immunoassay can be obtained applying widely more flexibly.This patent simplifies experimental procedure, shortens the time of experiment, can reduce experimental cost widely simultaneously.
Accompanying drawing explanation
Fig. 1 is experimental principle schematic flow sheet of the present utility model.
Embodiment
In order to make those skilled in the art can further understand feature of the present utility model and technology contents, refer to following about detailed description of the present utility model and accompanying drawing.
Refer to shown in Fig. 1, the utility model provides a kind of Novel immune trapped enzyme and urgees luminescence detection kit, described kit comprise pre-coated the immunocapture rod, antigen containing enzyme labeling or the antibody-solutions that have antigen or antibody the first reaction cup, the second reaction cup containing wash solution, strengthen liquid and H containing luminous substrate
2o
2the 3rd reaction cup, described immunocapture rod can be planted respectively and be active in first, second and third reaction cup described, and first, second and third reaction cup described is arranged independently of one another.
As preferably, described kit also includes the box body of a upper opening, and first, second and third reaction cup described is all located in box body.
Novel immune trapped enzyme inspires light and detects the experimental principle step of reagent and be: 1. be equipped with in the antigen of sample to be tested and enzyme labeling or the container of antibody-solutions by pre-coated for surface can being immersed in the immunocapture rod of the antigen of measured matter generation specific reaction or antibody, after insulation reaction a period of time, immunocapture rod can form the antigenantibody complex with enzyme marker mark; 2. the immunocapture rod obtaining the immune complex of enzyme marker mark is proposed, immerse in specific wash liquor vessel, remove and be attached to the free in conjunction with material or other interfering materials of spillikin surface, make the antigenantibody complex of only remaining enzyme labeling on immunocapture rod; 3. the immunocapture rod capturing the immune complex of enzyme marker mark through cleaning proposes by (also reaching the object of clean immunocapture rod by other modes), immerses luminous substrate (as luminol) and strengthens in liquid, add H
2o
2, label enzyme meeting catalysis Sum decomposition substrate is luminous, according to the monitoring to luminous intensity, can calculate the concentration of the analysis thing of sample.(experimental principle example as shown in Figure 1).
But the foregoing is only better possible embodiments of the present utility model, and be not used to limit to the scope of the claims of the present utility model, therefore the equivalent structure change that all utilization the utility model instructionss and accompanying drawing content are done, be all in like manner included in scope of the present utility model.
Claims (2)
1. a Novel immune trapped enzyme urgees luminescence detection kit, it is characterized in that, described kit comprise pre-coated the immunocapture rod, antigen containing enzyme labeling or the antibody-solutions that have antigen or antibody the first reaction cup, the second reaction cup containing wash solution, strengthen liquid and H containing luminous substrate
2o
2the 3rd reaction cup, described immunocapture rod can be planted respectively and be active in first, second and third reaction cup described, and first, second and third reaction cup described is arranged independently of one another.
2. Novel immune trapped enzyme as claimed in claim 1 urgees luminescence detection kit, and it is characterized in that, described kit also includes the box body of a upper opening, and first, second and third reaction cup described is all located in box body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520194714.0U CN204495830U (en) | 2015-04-02 | 2015-04-02 | Novel immune trapped enzyme urgees luminescence detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520194714.0U CN204495830U (en) | 2015-04-02 | 2015-04-02 | Novel immune trapped enzyme urgees luminescence detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204495830U true CN204495830U (en) | 2015-07-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520194714.0U Active CN204495830U (en) | 2015-04-02 | 2015-04-02 | Novel immune trapped enzyme urgees luminescence detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204495830U (en) |
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2015
- 2015-04-02 CN CN201520194714.0U patent/CN204495830U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: Cangshan District of Fuzhou City, Fujian province 350001 to build a new town, Jinzhou Road No. 10 a plant (No. 1) two (Fuzhou holding economic and Trade Development Co. Ltd.) Patentee after: Amis Technology Co Ltd Address before: Cangshan District of Fuzhou City, Fujian province 350001 to build a new town, Jinzhou Road No. 10 a plant (No. 1) two (Fuzhou holding economic and Trade Development Co. Ltd.) Patentee before: Foochow Mei Long medical science and technology company limited |
|
| CP01 | Change in the name or title of a patent holder |