CN217973230U - Magnetic head assembly and nucleic acid extractor - Google Patents
Magnetic head assembly and nucleic acid extractor Download PDFInfo
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- CN217973230U CN217973230U CN202221651895.1U CN202221651895U CN217973230U CN 217973230 U CN217973230 U CN 217973230U CN 202221651895 U CN202221651895 U CN 202221651895U CN 217973230 U CN217973230 U CN 217973230U
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Abstract
The utility model discloses a magnetic head assembly to a nucleic acid extraction instrument with magnetic head assembly is disclosed, including magnetic frame and many magnetic rods, the magnetic frame is used for this body coupling with nucleic acid extraction instrument. All the magnetic rods are arranged on the magnetic frame, the lower end heads of the magnetic rods are in an inverted round table shape or a conical shape, the lower ends of the magnetic rods can be inserted into the magnetic rod sleeves, and the outer surfaces of the lower end heads can be close to the inner conical walls on the lower ends of the magnetic rod sleeves. Because the lower end is in an inverted round table shape or a conical shape, the air magnetic resistance of each position of the outer surface of the lower end is similar to that of each position of the outer conical wall of the magnetic rod sleeve, namely the magnetic force of each position of the outer conical wall of the magnetic rod sleeve is similar, the magnetic rod can enable the magnetic beads in the sample solution to carry the nucleic acid to be attached to the outer conical wall of the lower end of the magnetic rod sleeve, the loss of the magnetic beads and the nucleic acid during transferring the magnetic beads is reduced, the bead obtaining rate is improved, the concentration of the nucleic acid is higher, and the detection of the nucleic acid is facilitated.
Description
Technical Field
The utility model relates to a biomedical equipment technical field, in particular to magnetic head subassembly and nucleic acid extraction appearance.
Background
The nucleic acid detection is to analyze the gene sequence of pathogenic microbe DNA or RNA for accurate diagnosis of clinical etiology, and the nucleic acid detection method is to detect by PCR amplification. The magnetic bead method for extracting nucleic acid can be generally divided into four steps: lysis, binding, washing and elution. At present, the magnetic piece can be used to transfer the magnetic beads in the sample tube into the reaction tube, and then the nucleic acid transferred into the reaction tube along with the magnetic beads is amplified and detected. In a general nucleic acid extractor, when a magnetic head of the nucleic acid extractor transfers magnetic beads, the magnetic beads are easily adsorbed on the outer circle side surface of a magnetic rod sleeve, so that the bead obtaining rate of the transferred magnetic beads is low, the concentration of nucleic acid is low, and the nucleic acid extractor is not beneficial to nucleic acid detection.
SUMMERY OF THE UTILITY MODEL
The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a magnetic head subassembly can improve the pearl rate for the concentration of nucleic acid is great, is favorable to improving the speed that nucleic acid detected and the degree of accuracy that detects.
The utility model also provides a nucleic acid extraction appearance of having above-mentioned magnetic head subassembly.
According to the utility model discloses magnetic head subassembly of first aspect embodiment, including magnetic shelf and many bar magnets, the magnetic shelf is used for this body coupling with the nucleic acid extraction appearance. All the magnetic rods are arranged on the magnetic frame, the lower end heads of the magnetic rods are in an inverted round table shape or a conical shape, the lower ends of the magnetic rods can be inserted into the magnetic rod sleeves, and the outer surfaces of the lower end heads can be close to the inner conical walls on the lower ends of the magnetic rod sleeves.
The method has the following beneficial effects: when the nucleic acid extractor needs to extract transferred nucleic acid, the lower end of the magnetic rod is inserted into the magnetic rod sleeve firstly, so that the outer surface of the lower end head is close to the inner conical wall of the lower end of the inner cavity, then the magnetic head assembly and the magnetic rod sleeve move downwards together, and the lower end of the magnetic rod sleeve extends into a sample solution of a sample test tube. Because the lower end is in an inverted round table shape or a conical shape, the air magnetic resistance at each position of the outer surface of the lower end is similar to that of the outer conical wall of the magnetic rod sleeve, namely the magnetic force at each position of the outer conical wall of the magnetic rod sleeve is similar, the phenomenon that the area between the outer conical wall and the outer side wall is the maximum magnetic force area is avoided, at the moment, the magnetic rod can enable magnetic beads in sample solution to carry nucleic acid to be attached to the outer conical wall at the lower end of the magnetic rod sleeve, and the magnetic beads are prevented from being attached to the area between the outer conical wall and the outer side wall and/or the outer side wall.
The magnetic beads carry nucleic acid to be attached to the outer conical wall at the lower end of the magnetic rod sleeve, so that the magnetic beads cannot fall off from the magnetic rod sleeve even if the outer side wall of the magnetic rod sleeve slightly collides and interferes with a sample test tube or a reaction tube or other parts when the magnetic head assembly and the magnetic rod sleeve are synchronously transferred; when the magnetic rod is drawn out from the magnetic rod sleeve, the magnetic pulling phenomenon is avoided, so that the magnetic beads are prevented from being firmly attached to the outer side wall of the magnetic rod sleeve. The magnetic head assembly finally enables the magnetic beads on the magnetic rod sleeve to fall into a reaction tube or other containers, reduces the loss of the magnetic beads and nucleic acid when the magnetic beads are transferred, improves the bead rate, further enables the concentration of the nucleic acid to be higher, and is beneficial to the detection of the nucleic acid.
According to some embodiments of the present invention, the taper of the lower end of the magnetic rod is 1:1-3:1.
According to some embodiments of the present invention, the magnetic poles of the lower ends of two adjacent magnetic rods are opposite.
According to some embodiments of the invention, the number of magnetic rods is any one of 48, 56, 64, 72, 80, 88, 96 and 102.
According to some embodiments of the utility model, the magnetic frame includes the mounting panel, be equipped with on the mounting panel a plurality of be used for with this body coupling's of nucleic acid extraction appearance connecting hole, and a plurality of confession the mounting hole of bar magnet installation.
According to some embodiments of the invention, the upper end of the magnetic bar is connected with the side wall of the mounting hole by a screw thread and/or an adhesive.
According to the utility model discloses a some embodiments, the magnetic frame still includes the apron, the upper surface of mounting panel is equipped with the holding tank, the top of bar magnet is equipped with keeps off the shoulder, the lower surface that keeps off the shoulder supports the tank bottom of holding tank, the apron with the mounting panel is connected and is covered on the holding tank, the lower surface of apron supports keep off the upper surface of shoulder with spacing keep off the shoulder.
According to a second aspect of the present invention, there is provided a nucleic acid extracting apparatus comprising a magnetic head assembly according to the first aspect of the present invention.
The method has the following beneficial effects: the nucleic acid extractor has all the advantages brought by the magnetic head assembly of the above embodiment, and the description thereof will not be repeated.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The invention will be further described with reference to the following drawings and examples, in which:
FIG. 1 is a schematic structural diagram of a magnetic head assembly according to an embodiment of the present invention;
FIG. 2 is a side view of a magnetic head assembly according to an embodiment of the present invention;
FIG. 3 is a schematic cross-sectional structural view of a magnetic head assembly according to an embodiment of the present invention;
fig. 4 is a partially enlarged schematic view of a portion a of fig. 3.
Reference numerals: the magnetic frame 100, the mounting plate 110, the connecting hole 111, the mounting hole 112, the receiving groove 113, the cover plate 120, the magnetic rod 200, the lower end head 210 and the blocking shoulder 220.
Detailed Description
Reference will now be made in detail to the embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present invention, and should not be construed as limiting the present invention.
In the description of the present invention, it should be understood that the orientation or positional relationship indicated with respect to the orientation description, such as up, down, front, rear, left, right, etc., is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the description of the present invention, a plurality means two or more. If there is a description of first and second for the purpose of distinguishing technical features only, this is not to be understood as indicating or implying a relative importance or implicitly indicating the number of technical features indicated or implicitly indicating the precedence of technical features indicated.
In the description of the present invention, unless there is an explicit limitation, the words such as setting, installation, connection, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in combination with the specific contents of the technical solution.
It can be known that the lower end of the existing magnetic rod sleeve is conical, the outer surface of the lower end of the magnetic rod sleeve is an outer conical wall, an inner cavity for the magnetic rod to extend into is arranged on the magnetic rod sleeve, the lower end of the inner cavity is also conical, the inner surface of the lower end of the inner cavity is an inner conical wall, and the outer cylindrical surface of the magnetic rod sleeve is an outer side wall. The lower extreme of current bar magnet is cylindricly, and the lower extreme of bar magnet can make the magnetic bead adsorb in the region between outer conical wall and the lateral wall.
Referring to fig. 1 and 2, the present invention discloses a magnetic head assembly, which comprises a magnetic frame 100 and a plurality of magnetic rods 200, wherein the magnetic frame 100 is used for connecting with a body of a nucleic acid extractor. All the magnetic rods 200 are arranged on the magnetic frame 100, the lower end heads 210 of the magnetic rods 200 are in an inverted round table shape or a conical shape, the lower ends of the magnetic rods 200 can be inserted into the magnetic rod sleeves, and the outer surfaces of the lower end heads 210 can be attached to the inner conical walls on the lower ends of the magnetic rod sleeves.
When the nucleic acid extractor needs to extract transferred nucleic acid, the lower end of the magnetic rod 200 is inserted into the magnetic rod sleeve, so that the outer surface of the lower end head 210 is close to the inner conical wall of the lower end of the inner cavity, and then the magnetic head assembly and the magnetic rod sleeve move downwards together, so that the lower end of the magnetic rod sleeve extends into a sample solution of a sample test tube. Because the lower end head 210 is in an inverted round table shape or a conical shape, the air magnetic resistance at each position of the outer surface of the lower end head 210 is similar to that of the outer conical wall of the magnetic rod sleeve, that is, the magnetic force at each position of the outer conical wall of the magnetic rod sleeve is similar, and the phenomenon that the area between the outer conical wall and the outer side wall is the maximum magnetic force area is avoided, at the moment, the magnetic rod 200 can enable magnetic beads in a sample solution to carry nucleic acid to be attached to the outer conical wall at the lower end of the magnetic rod sleeve, and the magnetic beads are prevented from being attached to the area between the outer conical wall and the outer side wall and/or the outer side wall.
The magnetic beads carry nucleic acid to be attached to the outer conical wall at the lower end of the magnetic rod sleeve, so that the magnetic beads cannot fall off from the magnetic rod sleeve even if the outer side wall of the magnetic rod sleeve slightly collides and interferes with a sample test tube or a reaction tube or other parts when the magnetic head assembly and the magnetic rod sleeve are synchronously transferred; when the magnetic rod 200 is drawn out from the magnetic rod sleeve, the magnetic pulling phenomenon is avoided, so that the magnetic beads are prevented from being firmly attached to the outer side wall of the magnetic rod sleeve. The magnetic head assembly finally enables the number of the magnetic beads on the magnetic rod sleeve to fall into a reaction tube or other containers, reduces the loss of the magnetic beads and nucleic acid when the magnetic beads are transferred, improves the bead rate, further enables the concentration of the nucleic acid to be higher, and is beneficial to the detection of the nucleic acid.
In another description of the angle, the outer circular sidewall of the lower end head 210 is formed with an inclined surface, any straight line parallel to the inclined surface and the center line of the magnetic rod 200 are in the same plane, and the straight line and the center line form an included angle, which is 30 ° to 71 °. Too small an angle may result in too sharp lower tip 210, and too large an angle may result in too blunt lower tip 210, so too small and too large an angle is not desirable.
It can be understood that, when the taper of the lower end head 210 is the same as the taper of the lower end of the magnetic rod sleeve, that is, when the distances between the outer surface of the lower end head 210 and the inner conical wall/outer conical wall of the magnetic rod sleeve are the same, the magnetic force at each position of the outer conical wall of the magnetic rod sleeve is uniform, which is beneficial for the magnetic beads to be adsorbed on the outer conical wall of the magnetic rod sleeve, and prevents the magnetic beads from being adsorbed on the region between the outer conical wall and the inner conical wall of the magnetic rod sleeve and/or the outer side wall of the magnetic rod sleeve.
In this embodiment, the taper of the lower end head 210 of the magnetic rod 200 is 1:1-3:1, and the taper range conforms to the existing magnetic rod sleeves with various specifications. Certainly, the taper of the lower end head 210 cannot be too large or too small, the outer wall of the magnetic rod 200 is firstly abutted against the inner side wall of the magnetic rod sleeve when the taper is too large, and the outer surface of the lower end head 210 is not close to the inner conical wall of the magnetic rod sleeve; an understeer tends to result in a central region of the outer surface of the lower tip 210 abutting a central region of the inner taper wall of the sleeve, while a non-central region of the outer surface of the lower tip 210 is not yet proximate the inner taper wall of the sleeve.
It can be understood that the magnetic poles of the lower ends 210 of two adjacent magnetic rods 200 are opposite, that is, a magnetic field loop can be formed between the lower ends 210 of two adjacent magnetic rods 200, so that the magnetic force of the lower ends 210 is greater, and the ability of the lower ends 210 to adsorb magnetic beads is stronger.
It is understood that the number of the magnetic rods 200 is any one of 48, 56, 64, 72, 80, 88, 96 and 102, and the number of the magnetic rods 200 can be set according to actual situations. Of course, the diameter and length of the magnetic rod 200 may be set according to actual conditions. The diameter of the magnetic rod 200 may be 2mm to 8mm, and the length of the magnetic rod 200 may be 7cm to 20cm.
It is understood that the magnetic frame 100 includes a mounting plate 110, the mounting plate 110 is provided with a plurality of connecting holes 111 for connecting with the body of the nucleic acid extractor, and the entire magnetic head assembly can be conveniently and rapidly mounted on or dismounted from the body of the nucleic acid extractor through the connecting holes 111. The mounting plate 110 is further provided with a plurality of mounting holes 112 for mounting the magnetic rods 200, and the upper ends of the magnetic rods 200 and the mounting holes 112 are used for mounting the magnetic rods 200 on the mounting plate 110.
It is understood that the upper end of the magnetic rod 200 is connected to the sidewall of the mounting hole 112 by means of screw and/or glue. When the magnetic rod 200 and the mounting plate 110 are connected in a threaded connection mode, the upper end of the magnetic rod 200 is provided with an external thread, the mounting hole 112 is a threaded hole, and the external thread is matched with the internal thread of the threaded hole. When the magnetic rod 200 is connected with the mounting plate 110 by using the adhesive, the upper end of the magnetic rod 200 is inserted into the mounting hole 112, and then the adhesive is filled between the upper end of the magnetic rod 200 and the inner wall of the mounting hole 112.
Referring to fig. 3 and 4, the magnetic frame 100 further includes a cover plate 120, the upper surface of the mounting plate 110 is provided with a receiving groove 113, the mounting hole 112 is opened at the bottom wall of the receiving groove 113, that is, the receiving groove 113 is communicated with the mounting hole 112, and the upper end of the magnetic rod 200 is provided with a shoulder 220. When the magnetic rod 200 is installed, the lower end of the magnetic rod 200 penetrates through the installation hole 112 from top to bottom, and the lower surface of the shoulder 220 is abutted to the bottom of the accommodating groove 113, so that the magnetic rod 200 is limited on the magnetic frame 100.
The cover plate 120 is connected to the mounting plate 110 and covers the receiving groove 113, and when the cover plate 120 is connected to the mounting plate 110, the lower surface of the cover plate 120 abuts against the upper surface of the shoulder 220, so as to limit the magnetic rod 200 on the magnetic frame 100, and the inner wall of the mounting hole 112 limits the magnetic rod 200 on the magnetic frame 100 in the horizontal direction, so that the magnetic rod 200 is fixed on the magnetic frame 100.
The cover plate 120 and the mounting plate 110 may be coupled together by fasteners, such as screws. Two sections of the connection hole 111 are formed on the cover plate 120 and the mounting plate 110, respectively, that is, the connection hole 111 penetrates through the cover plate 120 and the mounting plate 110, and when the body of the nucleic acid extracting apparatus is connected to the magnetic head assembly having the connection hole 111, both the cover plate 120 and the mounting plate 110 can be connected to the body of the nucleic acid extracting apparatus.
The utility model discloses a nucleic acid extraction instrument, including the body and the foretell magnetic head subassembly of nucleic acid extraction instrument, the body of nucleic acid extraction instrument includes the frame, is equipped with in the frame to be used for taking advantage of carrying of containers such as sample test tube and reaction tube and carries the mechanism, still is equipped with in the frame to be used for driving the actuating mechanism that magnetic head subassembly and bar magnet cover removed.
The nucleic acid extractor has all the advantages brought by the magnetic head assembly of the above embodiment, and the description thereof will not be repeated.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
Of course, the present invention is not limited to the above-mentioned embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the present invention, and such equivalent modifications or substitutions are included in the scope defined by the claims of the present application.
Claims (8)
1. A magnetic head assembly, comprising:
the magnetic frame is used for being connected with the body of the nucleic acid extractor;
the magnetic rods are arranged on the magnetic frame, the lower end heads of the magnetic rods are in an inverted round table shape or a conical shape, the lower ends of the magnetic rods can be inserted into the magnetic rod sleeve, and the outer surfaces of the lower end heads can be close to the inner conical wall on the lower end of the magnetic rod sleeve.
2. The magnetic head assembly of claim 1, wherein: the taper of the lower end of the magnetic rod is 1:1-3:1.
3. A magnetic head assembly according to claim 1 or 2, wherein: the magnetic poles of the lower ends of two adjacent magnetic rods are opposite.
4. A magnetic head assembly according to claim 1 or 2, wherein: the number of the magnetic rods is any one of 48, 56, 64, 72, 80, 88, 96 and 102.
5. The magnetic head assembly of claim 1, wherein: the magnetic frame comprises a mounting plate, wherein the mounting plate is provided with a plurality of connecting holes for connecting with the nucleic acid extractor body and a plurality of mounting holes for mounting the magnetic rod.
6. The magnetic head assembly of claim 5, wherein: the upper end of the magnetic rod is connected with the side wall of the mounting hole through threads and/or adhesive.
7. The magnetic head assembly of claim 5, wherein: the magnetism frame still includes the apron, the upper surface of mounting panel is equipped with the holding tank, the top end of bar magnet is equipped with keeps off the shoulder, the lower surface that keeps off the shoulder supports the tank bottom of holding tank, the apron with the mounting panel is connected and is covered on the holding tank, the lower surface of apron supports keep off the upper surface of shoulder with spacing keep off the shoulder.
8. Nucleic acid extractor, its characterized in that: a magnetic head assembly comprising any of the preceding claims 1 to 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221651895.1U CN217973230U8 (en) | 2022-06-28 | 2022-06-28 | Magnetic head assembly and nucleic acid extractor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221651895.1U CN217973230U8 (en) | 2022-06-28 | 2022-06-28 | Magnetic head assembly and nucleic acid extractor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN217973230U true CN217973230U (en) | 2022-12-06 |
| CN217973230U8 CN217973230U8 (en) | 2023-02-07 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221651895.1U Active CN217973230U8 (en) | 2022-06-28 | 2022-06-28 | Magnetic head assembly and nucleic acid extractor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217973230U8 (en) |
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- 2022-06-28 CN CN202221651895.1U patent/CN217973230U8/en active Active
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| Publication number | Publication date |
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| CN217973230U8 (en) | 2023-02-07 |
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Legal Events
| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CU01 | Correction of utility model |
Correction item: Patentee|Address|Patentee Correct: ZHUHAI HEMA MEDICAL INSTRUMENT Co.,Ltd.|No.2, Keji 2nd Road, Gangwan Avenue, Tangjiawan Town, Zhuhai City, Guangdong Province 519000|ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. False: Zhuhai Heima Medical Co.,Ltd.|No.2, Keji 2nd Road, Gangwan Avenue, Tangjiawan Town, Zhuhai City, Guangdong Province 519000|ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. Number: 49-01 Volume: 38 Correction item: Patentee|Address|Patentee Correct: ZHUHAI HEMA MEDICAL INSTRUMENT Co.,Ltd.|No.2, Keji 2nd Road, Gangwan Avenue, Tangjiawan Town, Zhuhai City, Guangdong Province 519000|ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. False: Zhuhai Heima Medical Co.,Ltd.|No.2, Keji 2nd Road, Gangwan Avenue, Tangjiawan Town, Zhuhai City, Guangdong Province 519000|ZHUHAI BLACK HORSE BIOTECHNOLOGY Co.,Ltd. Number: 49-01 Page: The title page Volume: 38 |
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| CU01 | Correction of utility model |