CN220056778U - TIP head imbibition observation device for nucleic acid purification amplification detection integrated machine - Google Patents

Abstract

The utility model discloses a TIP head imbibition observation device for a nucleic acid purification, amplification and detection integrated machine, which comprises a hanging plate, wherein the hanging plate is in a door-shaped structure and is provided with a clamping and hanging cavity with a lower opening; the perforation is in a rectangular structure and is arranged on the hanging plate; the observation tube longitudinally penetrates through the perforation, the upper end of the observation tube is communicated with an upper observation port which is horizontally arranged, the lower end of the observation tube is communicated with a lower observation port which is horizontally arranged, a first plane mirror is obliquely arranged in the upper observation port, and a second plane mirror is obliquely arranged in the lower observation port; the vertical plate is vertically and fixedly connected to one side edge of the perforation; the jacking bolt is in threaded connection and penetrates through the vertical plate, one end of the jacking bolt is a holding end, and the other end of the jacking bolt is a rubber anti-slip end. The utility model has the advantages that the liquid absorption end position of the TIP head is easily observed by utilizing the secondary reflection of the first plane mirror and the second plane mirror, the TIP head is convenient to debug and confirm, the operation error is reduced, the TIP head is ensured to be at the optimal position, and the detection efficiency of the nucleic acid purification amplification detection integrated machine is improved.

Description

TIP head imbibition observation device for nucleic acid purification amplification detection integrated machine

Technical Field

The utility model relates to the technical field of in-vitro diagnosis, in particular to a TIP head imbibition observation device for a nucleic acid purification, amplification and detection integrated machine.

Background

Polymerase Chain Reaction (PCR) is a technique for rapid amplification of specific DNA or RNA fragments in vitro. The integrated nucleic acid purification amplification detection machine designed based on the polymerase chain reaction technology is a common in-vitro diagnostic instrument in the market at present, and is an integrated instrument capable of extracting, purifying, amplifying and detecting nucleic acid; during the construction of an amplification system, an object to be detected is subjected to cracking, incubation and magnetic attraction washing, and then is extracted by a liquid sucking needle (namely a TIP head) of an instrument through a preparation channel, so that the construction of the amplification system is completed.

At present, when a TIP head of the nucleic acid purification amplification detection integrated machine stretches into a preparation channel to suck liquid, the position of a liquid sucking end of the TIP head in the preparation channel is a key for determining whether the liquid sucking of an instrument is in place, and the subsequent amplification reaction result is influenced by the fact that the liquid sucking end cannot be used or the liquid sucking end cannot be used, so that the detection result difference is large, and the reagent reaction result is greatly influenced. Therefore, in order to ensure the detection accuracy, it is common to perform debugging and confirmation of the position of the liquid sucking end of the TIP head extending into the preparation channel, so as to ensure that the TIP head can sufficiently suck the liquid in the preparation channel. However, as the side of the preparation channel on the instrument is provided with the separate injection channels (such as an Automolecular 3000 type full-automatic nucleic acid purification and real-time fluorescence PCR analysis system) at intervals side by side, the space around the preparation channel is limited, the observation is inconvenient, the TIP head position needs to be photographed and observed by stretching a mobile phone into a gap between the preparation channel and the separate injection channels every time when the TIP head is debugged, the focusing is extremely difficult due to the narrow space when the mobile phone photographs, the operation risk is higher, the operation error is increased, and the TIP head cannot be ensured to be positioned at the optimal position.

Disclosure of Invention

The utility model aims to provide a TIP head imbibition observation device for a nucleic acid purification, amplification and detection integrated machine.

In order to achieve the above purpose, the present utility model may adopt the following technical scheme:

the utility model relates to a TIP head imbibition observation device for a nucleic acid purification amplification detection integrated machine, which comprises the following components:

the hanging plate is in a door-shaped structure and is provided with a lower opening clamping and hanging cavity which is adapted to be clamped and hung on the preparation channel and the dispensing channel;

the perforation is in a rectangular structure and is arranged on the hanging plate, and the perforation is right above a gap between the preparation channel and the dispensing channel;

the observation tube longitudinally penetrates through the perforation, an upper observation port which is horizontally arranged is communicated with the upper end of the observation tube, a lower observation port which is horizontally arranged is communicated with the lower end of the observation tube, a first plane mirror is obliquely arranged in the upper observation port, and a second plane mirror corresponding to the first plane mirror is obliquely arranged in the lower observation port;

the vertical plate is vertically and fixedly connected to one side edge of the perforation;

the jacking bolt is in threaded connection and penetrates through the vertical plate, one end of the jacking bolt is a holding end, and the other end of the jacking bolt is a rubber anti-slip end propped against the outer side wall of the observation cylinder.

The utility model has the advantages that the hanging plate with the portal-shaped structure is easily clamped and hung on the preparation channel and the separate injection channel of the nucleic acid purification and amplification detection integrated machine, so that the observation tube penetrating through the perforation can extend into the gap between the preparation channel and the separate injection channel, the hanging plate is moved along the length direction of the preparation channel and the separate injection channel, the observation tube is adjusted up and down, the lower observation port at the lower end of the observation tube is opposite to the liquid absorption end of the TIP head extending into the preparation channel, the position of the liquid absorption end of the TIP head can be easily observed by utilizing the secondary reflection of the first plane mirror and the second plane mirror, the debugging and the confirmation of the TIP head are convenient, the problems of high focusing difficulty, high operation risk and the like when the mobile phone is photographed are effectively solved, the operation error is reduced, the TIP head is ensured to be at the optimal position, and the detection efficiency of the nucleic acid purification and amplification detection integrated machine is improved.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is an enlarged view of the hanger plate of fig. 1.

Fig. 3 is an enlarged view of the observation barrel of fig. 1.

Fig. 4 is a front view of fig. 3.

Fig. 5 is a cross-sectional view taken along A-A of fig. 4.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.

Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature.

1-5, the TIP head imbibition observation device for the nucleic acid purification and amplification detection integrated machine comprises a hanging plate 1 with a door-shaped structure, wherein the hanging plate 1 is provided with a lower opening clamping and hanging cavity 4 which is adapted to be clamped and hung on a preparation channel 2 and a dispensing channel 3 of the nucleic acid purification and amplification detection integrated machine; a through hole 5 is formed in the transverse plate body of the hanging plate 1, and the through hole 5 is in a rectangular structure and is right above a gap between the preparation channel 2 and the dispensing channel 3.

An observation tube 6 is longitudinally penetrated in the perforation 5, the observation tube 6 is a rectangular tube body, and the size of the cross section of the observation tube 6 is matched with the size of the perforation 5; the upper end of the observation tube 6 is communicated with an upper observation port 7 which is horizontally arranged, the lower end of the observation tube is communicated with a lower observation port 8 which is horizontally arranged, the orientation of the upper observation port 7 is consistent with that of the lower observation port 8, and when the observation tube 6 is inserted into the perforation 5, the lower observation port 8 is just guaranteed to face the side of the preparation channel 2. In addition, a first plane mirror 9 is obliquely arranged in the upper observation port 7, a second plane mirror 10 corresponding to the first plane mirror 9 is obliquely arranged in the lower observation port 8, the mirror surface of the first plane mirror 9 faces downwards, the mirror surface of the second plane mirror 10 faces upwards, and the inclination angles of the first plane mirror 9 and the second plane mirror 10 ensure that an image reflected from the lower observation port 8 is reflected by the second plane mirror 10, passes through the observation cylinder 6 and is mapped on the first plane mirror 9, and then is reflected by the first plane mirror 9 and then is transmitted from the upper observation port 7, so that the image at the lower observation port 8 can be clearly observed through the upper observation port 7.

In addition, a vertical plate 11 is further vertically arranged at one side edge of the through hole 5, a tightening bolt 12 is threaded on the vertical plate 11, one end of the tightening bolt 12 is a holding end, the other end of the tightening bolt is a rubber anti-slip end propped against the outer side wall of the observation cylinder 6, the tightening bolt 12 is screwed to enable the rubber anti-slip end to be tightly propped against the outer side wall of the observation cylinder 6, so that the observation cylinder 6 is fixed, and the tightening bolt 12 is reversely screwed to enable the rubber anti-slip end to be far away from the observation cylinder 6, so that the observation cylinder 6 can conveniently slide up and down in the through hole 5 to adjust the height.

When the device is used, as shown in fig. 1, the device is clamped and hung on a preparation channel 2 and a dispensing channel 3 of the nucleic acid purification, amplification and detection integrated machine only through a hanging plate 1, and at the moment, an observation cylinder 6 extends into a gap between the preparation channel 2 and the dispensing channel 3; then the hanging plate 1 is slid along the length direction of the preparation channel 2 and the dispensing channel 3, and the observation tube 6 is adjusted up and down, so that the lower observation port 8 at the lower end of the observation tube 6 just faces the liquid suction end of the TIP head 13 extending into the preparation channel 2; because the preparation channel 2 and the dispensing channel 3 are of frame-shaped structures, images in the lower observation port 8 can be clearly observed from the upper observation port 7 through secondary reflection of the first plane mirror 9 and the second plane mirror 10, so that easy observation of the liquid suction end position of the TIP head 13 extending into the preparation channel 2 is realized, the liquid suction end position of the TIP head 13 is conveniently determined, the height position of the TIP head 13 is conveniently adjusted, the problems of high focusing difficulty, high operation risk and the like when a mobile phone is photographed are effectively solved, operation errors are reduced, the TIP head 13 is ensured to be in an optimal position, and the detection efficiency of the nucleic acid purification, amplification and detection integrated machine is improved.

Claims (1)

1. A liquid suction observation device of a TIP head for a nucleic acid purification amplification detection integrated machine is characterized in that: comprising

The hanging plate is in a door-shaped structure and is provided with a lower opening clamping and hanging cavity which is adapted to be clamped and hung on the preparation channel and the dispensing channel;

the perforation is in a rectangular structure and is arranged on the hanging plate, and the perforation is right above a gap between the preparation channel and the dispensing channel;

the observation tube longitudinally penetrates through the perforation, an upper observation port which is horizontally arranged is communicated with the upper end of the observation tube, a lower observation port which is horizontally arranged is communicated with the lower end of the observation tube, a first plane mirror is obliquely arranged in the upper observation port, and a second plane mirror corresponding to the first plane mirror is obliquely arranged in the lower observation port;

the vertical plate is vertically and fixedly connected to one side edge of the perforation;

the jacking bolt is in threaded connection and penetrates through the vertical plate, one end of the jacking bolt is a holding end, and the other end of the jacking bolt is a rubber anti-slip end propped against the outer side wall of the observation cylinder.