CN215162534U

CN215162534U - Free nucleic acid extractor

Info

Publication number: CN215162534U
Application number: CN202120357909.8U
Authority: CN
Inventors: 朱美玲; 刘和录; 张大威; 赖文清; 窦宇红; 杜纪坤; 司文文; 杨林
Original assignee: Shenzhen Integrated Traditional Chinese And Western Medicine Hospital
Current assignee: Shenzhen Integrated Traditional Chinese And Western Medicine Hospital
Priority date: 2021-02-08
Filing date: 2021-02-08
Publication date: 2021-12-14
Anticipated expiration: 2031-02-08

Abstract

The application discloses free nucleic acid extraction appearance. The free nucleic acid extraction instrument comprises a base, a heating plate arranged on the base, a plurality of extraction box bodies inserted on the heating plate, a top seat positioned above the extraction box bodies, a transverse moving plate slidably mounted on the base, a transverse moving driving mechanism used for driving the transverse moving plate to move, a first lifting frame slidably mounted on the transverse moving plate, a first lifting driving mechanism used for driving the first lifting frame to move, a second lifting frame slidably mounted on the transverse moving plate, and a second lifting driving mechanism used for driving the second lifting frame to move; the first lifting frame is provided with a plurality of sleeves, and the second lifting frame is provided with a plurality of magnetic rods opposite to the sleeves. Due to the arrangement of the heating plate, liquid in the extracting box body can be actively heated, after the liquid in the extracting box body is heated, the molecular motion speed is accelerated, the contact speed of nucleic acid and magnetic beads is accelerated, and the nucleic acid is quickly adsorbed on the magnetic beads. The number of times of lifting and stirring of the sleeve can be reduced, and the nucleic acid extraction speed is accelerated.

Description

Free nucleic acid extractor

Technical Field

The application relates to the technical field of nucleic acid extraction, in particular to a free nucleic acid extractor.

Background

Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.

Generally, the extraction steps for extracting nucleic acid by the magnetic bead method mainly comprise: (1) cracking; (2) combining; (3) rinsing; (4) and (4) eluting. Specifically, the DNA/RNA is released from cells or tissues under the action of a lysis solution, and the surface-modified superparamagnetic silica nano magnetic beads are specifically combined with the released DNA/RNA to form a nucleic acid-magnetic bead compound. Adding a rinsing liquid into the nucleic acid-magnetic bead compound, washing off impurities such as non-specifically adsorbed protein and polysaccharide, separating the nucleic acid-magnetic bead compound with the impurities removed from the magnetic bead in an eluent, and finally obtaining a nucleic acid substance to be extracted. If the steps of cracking, combining, rinsing and eluting are not mixed uniformly in the nucleic acid extraction process, the reagent is not fully contacted with the sample, the intracellular nucleic acid is not completely released, the magnetic beads are easy to settle, and the extraction efficiency is greatly reduced. However, the existing free nucleic acid extractor has low nucleic acid extraction rate, and cannot extract nucleic acid quickly and effectively.

SUMMERY OF THE UTILITY MODEL

The application aims to provide a free nucleic acid extractor, and aims to solve the problem that the extraction rate of the free nucleic acid extractor is low in the prior art.

To achieve the purpose, the embodiment of the application adopts the following technical scheme:

the free nucleic acid extraction instrument comprises a base, a heating plate arranged on the base, a plurality of extraction box bodies inserted on the heating plate, a top seat positioned above the extraction box bodies, a transverse moving plate slidably mounted on the base, a transverse moving driving mechanism used for driving the transverse moving plate to move, a first lifting frame slidably mounted on the transverse moving plate, a first lifting driving mechanism used for driving the first lifting frame to move, a second lifting frame slidably mounted on the transverse moving plate, and a second lifting driving mechanism used for driving the second lifting frame to move; the first lifting frame is provided with a plurality of sleeves, and the second lifting frame is provided with a plurality of magnetic rods opposite to the sleeves; the magnetic rods are in one-to-one correspondence with the sleeves, and the magnetic rods can be inserted into the sleeves.

In one embodiment, a vibration structure is further arranged in the base, and the vibration structure can drive the extraction box body to vibrate.

In one embodiment, the extraction cartridge has a first extraction duct and a plurality of second extraction duct; the first extraction pipe groove and the plurality of second extraction pipe grooves are arranged in sequence and at intervals along a preset direction.

In one embodiment, the heating plate is provided with a plurality of mounting grooves, and the extraction box body is inserted into the mounting grooves.

In one embodiment, an end of the cannula distal to the first crane has a tip portion; the surface of the tip part is an arc surface.

In one embodiment, the first lifting driving mechanism and the second lifting driving mechanism are respectively arranged on two opposite sides of the traverse board.

In one embodiment, the first lifting driving mechanism comprises a first driving pulley rotatably mounted on the traverse plate, a first driven pulley rotatably mounted on the traverse plate, a first driving belt connected between the first driving pulley and the first driven pulley, and a first lifting motor mounted on the traverse plate and used for driving the first driving pulley to rotate; the first lifting frame is connected with the first transmission belt.

In one embodiment, the first elevation driving mechanism further comprises a first elevation guide rail mounted to the traverse plate, a first elevation slider slidably mounted to the first elevation guide rail; the first lifting frame is connected with the first lifting slide block; the transverse moving plate is provided with a first lifting position sensor, and the first lifting frame is provided with a first lifting trigger piece used for triggering the first lifting position sensor.

In one embodiment, the second lift driving mechanism includes a second driving pulley rotatably mounted to the traverse plate, a second driven pulley rotatably mounted to the traverse plate, a second transmission belt connected between the second driving pulley and the second driven pulley, and a second lift motor mounted to the traverse plate and configured to drive the second driving pulley to rotate.

In one embodiment, the second elevation driving mechanism further comprises a second elevation guide rail mounted to the traverse plate, a second elevation slider slidably mounted to the second elevation guide rail; the second lifting frame is connected with the second lifting slide block; and a second lifting position sensor is arranged on the transverse moving plate, and a second lifting trigger piece used for triggering the second lifting position sensor is arranged on the second lifting frame.

In one embodiment, the traverse driving mechanism comprises a traverse driving pulley rotatably mounted on the top base, a traverse driven pulley rotatably mounted on the top base, a traverse transmission belt connected between the traverse driving pulley and the traverse driven pulley, and a traverse motor mounted on the top base and configured to drive the traverse driving pulley to rotate; the transverse moving plate is connected with the transverse moving transmission belt.

In one embodiment, a transverse moving position sensor is arranged on the top seat, and a transverse moving trigger sheet for triggering the transverse moving position sensor is arranged on the transverse moving plate.

In one embodiment, the traverse driving mechanism further comprises a traverse guide rail mounted on the top seat, and a traverse slider slidably mounted on the traverse guide rail; the transverse moving plate is connected with the transverse moving slide block.

The beneficial effects of the embodiment of the application are as follows: the heating plate works to generate heat and transfer the heat to the extraction box body, and the liquid in the extraction box body is heated. After the liquid in the extraction box body is heated, the molecular motion speed is accelerated, the contact speed of nucleic acid and magnetic beads is accelerated, and the nucleic acid is quickly adsorbed on the magnetic beads. The transverse moving driving mechanism drives the transverse moving plate to transversely move, so that the sleeve on the first lifting frame is over against the extraction box body, then the first lifting driving mechanism drives the first lifting frame to do lifting motion, the sleeve stretches into the extraction box body, liquid in the extraction box body is stirred, and the contact degree of magnetic beads and nucleic acids is further improved. When the nucleic acid is fully contacted with the magnetic beads, the second lifting driving mechanism drives the second lifting frame to descend, so that the magnetic rod is inserted into the sleeve, and the magnetic beads with the nucleic acid are adsorbed on the surface of the sleeve by the magnetic rod through the sleeve.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic diagram of a free nucleic acid extractor according to an embodiment of the present disclosure;

FIG. 2 is a schematic view of a top mount top structure according to an embodiment of the present disclosure;

FIG. 3 is a schematic diagram illustrating a second perspective view of a structure on a top mount according to an embodiment of the present application;

FIG. 4 is a schematic view from a third perspective of a structure on a top mount according to an embodiment of the present application;

in the figure:

1. a base; 2. heating plates; 201. mounting grooves; 3. extracting the box body; 301. a first extraction duct tank; 302. a second extraction duct; 4. a top seat; 5. a traverse driving mechanism; 501. transversely moving a driving belt wheel; 502. transversely moving the driven belt wheel; 503. transversely moving the transmission belt; 504. a traversing motor; 505. transversely moving the guide rail; 506. transversely moving the sliding block; 6. a first elevation drive mechanism; 601. a first driving pulley; 602. a first driven pulley; 603. a first drive belt; 604. a first lift motor; 605. a first lifting guide rail; 606. a first lifting slider; 7. a second elevation drive mechanism; 701. a second driving pulley; 702. a second driven pulley; 703. a second belt; 704. a second lift motor; 705. a second lifting guide rail; 706. a second lifting slide block; 8. a sleeve; 10. transversely moving the plate; 11. a first lifting frame; 12. a second lifting frame; 14. a magnetic bar; 15. a first elevation position sensor; 16. a first lift trigger tab; 17. a second elevation position sensor; 18. a second lift trigger tab; 19. a lateral movement position sensor; 20. the trigger piece is moved laterally.

Detailed Description

In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, refer to an orientation or positional relationship illustrated in the drawings for convenience in describing the present application and to simplify description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present application.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

The following detailed description of implementations of the present application is provided in conjunction with specific embodiments.

As shown in fig. 1-3, the present application provides a free nucleic acid extracting apparatus, which includes a base 1, a heating plate 2 disposed on the base 1, a plurality of extracting box bodies 3 inserted on the heating plate 2, a top seat 4 located above the extracting box bodies 3, a traverse plate 10 slidably mounted on the base 1, a traverse driving mechanism 5 for driving the traverse plate 10 to move, a first lifting frame 11 slidably mounted on the traverse plate 10, a first lifting driving mechanism 6 for driving the first lifting frame 11 to move, a second lifting frame 12 slidably mounted on the traverse plate 10, and a second lifting driving mechanism 7 for driving the second lifting frame 12 to move; the first lifting frame 11 is provided with a plurality of sleeve pipes 8, and the second lifting frame 12 is provided with a plurality of magnetic rods 14 opposite to the sleeve pipes 8; the magnetic rods 14 correspond to the sleeves 8 one by one, and the magnetic rods 14 can be inserted into the sleeves 8.

In the embodiment of the present application, a cell or tissue solution (having nucleic acids) is disposed in the extraction cartridge 3, and meanwhile, magnetic beads, a lysis solution, a binding solution, a rinsing solution or an eluent is disposed, the lysis solution is used for releasing (nucleic acid) DNA/RNA in the cell or tissue solution by lysis, the binding solution is used for binding the magnetic beads and the nucleic acids, the rinsing solution is used for washing impurities such as non-specifically adsorbed proteins and polysaccharides in the nucleic acids, the number of washing times is generally 1-3 times, the nucleic acids can be washed clean, and the eluent is used for eluting the nucleic acids from the magnetic beads, so as to complete the extraction of the nucleic acids.

The working process of the free nucleic acid extractor is as follows: the heating plate 2 works, generating heat and transferring it to the extraction cartridge 3, the liquid inside the cartridge is heated. After the liquid in the extraction box body 3 is heated, the molecular motion speed is accelerated, the contact speed of nucleic acid and magnetic beads is accelerated, and the nucleic acid is quickly adsorbed on the magnetic beads. The transverse moving driving mechanism 5 drives the transverse moving plate 10 to transversely move, so that the sleeve 8 on the first lifting frame 11 is over against the extracting box body 3, then the first lifting driving mechanism 6 drives the first lifting frame 11 to do lifting motion, the sleeve 8 stretches into the extracting box body 3, liquid in the extracting box body 3 is stirred, and the contact degree of magnetic beads and nucleic acids is further improved. After the nucleic acid is fully contacted with the magnetic beads, the second lifting driving mechanism 7 drives the second lifting frame 12 to descend, so that the magnetic rod 14 is inserted into the sleeve 8, and the magnetic rod 14 adsorbs the magnetic beads with the nucleic acid on the surface of the sleeve 8 through the sleeve 8.

Because the setting of hot plate 2, can be initiative to extracting the liquid heating in the box body 3, extract the liquid in the box body 3 back by the heating, the molecular motion speed accelerates, accelerates the speed of nucleic acid and magnetic bead contact for nucleic acid adsorbs on the magnetic bead fast. The number of times of lifting and stirring of the sleeve 8 can be reduced, the extraction time is saved, and the nucleic acid extraction speed is accelerated.

Referring to fig. 1, as another embodiment of the free nucleic acid extractor provided in the present application, a vibration structure is further disposed in the base 1, and the vibration structure can drive the extraction box 3 to vibrate, so as to accelerate movement of liquid in the extraction box, accelerate a rate of contact between nucleic acid and magnetic beads, and further increase a rate of nucleic acid extraction.

Referring to fig. 1, as another embodiment of the free nucleic acid extractor provided in the present application, the extracting box 3 has a first extracting pipe slot 301 and a plurality of second extracting pipe slots 302; the first extraction pipe groove 301 and the plurality of second extraction pipe grooves 302 are sequentially arranged at intervals along a preset direction (the transverse movement driving mechanism 5 drives the first lifting frame 11 and the second lifting frame 12 to transversely move, so that the sleeve 8 is sequentially aligned with the first extraction pipe groove 301 and the second extraction pipe grooves 302). For example, three second extraction duct slots 302: the first extraction pipe tank 301 stores sample reagent, lysis solution and binding solution, and rinsing solution, rinsing solution and eluent are sequentially stored in the three second extraction pipe tanks 302, namely, two times of rinsing and one time of elution are needed in the nucleic acid extraction process.

Referring to fig. 1, as another embodiment of the free nucleic acid extractor provided in the present application, a plurality of mounting grooves 201 are formed on a heating plate 2, and an extracting box 3 is inserted into the mounting grooves 201. On the one hand, it is spacing to draw box body 3, avoids drawing the removal of box body 3, and on the other hand promotes the heating rod and draws the area of box body 3 contact for be heated, promote the speed that the nucleic acid drawed.

Referring to fig. 1, as another embodiment of the free nucleic acid extractor provided by the present application, one end of the cannula 8 away from the first lifting frame 11 has a tip portion, which can make the cannula 8 extend deeper, make more magnetic beads contact, and extract more nucleic acids at one time; the surface of the tip part is an arc surface, so that the surface area of the tip part is further increased, the contact area with magnetic beads is increased, and the quantity of nucleic acid extracted at one time is more.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided in the present application, the first lifting driving mechanism 6 and the second lifting driving mechanism 7 are respectively disposed at two opposite sides of the traverse plate 10, so as to effectively reduce the width of the traverse plate 10, and integrate the first lifting mechanism and the second lifting mechanism in a smaller space range, so that the structure of the free nucleic acid extractor is more compact.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided by the present application, the first lifting driving mechanism 6 includes a first driving pulley 601 rotatably mounted on the traverse plate 10, a first driven pulley 602 rotatably mounted on the traverse plate 10, a first driving belt 603 connected between the first driving pulley 601 and the first driven pulley 602, and a first lifting motor 604 mounted on the traverse plate 10 and configured to drive the first driving pulley 601 to rotate; the first crane 11 is connected to the first belt 603. The first lifting motor 604 drives the first driving pulley 601 to rotate, and then drives the first transmission belt 603 to move in cooperation with the first driven pulley 602, and the first transmission belt 603 drives the first lifting frame 11 to move when moving, so as to drive the first lifting frame 11 to move in the vertical direction.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided in the present application, the first elevation driving mechanism 6 further includes a first elevation guiding rail 605 installed on the traverse plate 10, and a first elevation slider 606 slidably installed on the first elevation guiding rail 605; the first lifting frame 11 is connected with the first lifting slide block 606; therefore, when the first lifting frame 11 moves in the vertical direction, the first lifting frame approaches to move only along the length direction of the first lifting guide rail 605, so that the first lifting frame is not easy to shift, and the stability during lifting is improved. Be equipped with first lift position sensor 15 (can be a plurality of that set gradually) on the sideslip board 10, be equipped with on the first crane 11 and be used for triggering first lift position sensor 15's first lift trigger piece 16 for detect the height that first crane 11 goes up and down, avoid the position of going up and down excessive.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided by the present application, the second lifting driving mechanism 7 includes a second driving pulley 701 rotatably mounted on the traverse plate 10, a second driven pulley 702 rotatably mounted on the traverse plate 10, a second belt 703 connected between the second driving pulley 701 and the second driven pulley 702, and a second lifting motor 704 mounted on the traverse plate 10 and configured to drive the second driving pulley 701 to rotate. The second lifting motor 704 drives the second driving pulley 701 to rotate, and then drives the second driving belt 703 to move in cooperation with the second driven pulley 702, and the second driving belt 703 drives the second lifting frame 12 to move when moving, so as to drive the second lifting frame 12 to move in the vertical direction.

Referring to fig. 2 to 4, as another embodiment of the free nucleic acid extractor provided in the present application, the second lifting driving mechanism 7 further includes a second lifting guide 705 installed on the traverse plate 10, and a second lifting slider 706 slidably installed on the second lifting guide 705; the second lifting frame 12 is connected with a second lifting slide block 706; therefore, when the second lifting frame 12 moves in the vertical direction, the second lifting frame approaches to move only along the length direction of the second lifting guide rail 705, and the second lifting frame is not easy to shift, so that the stability during lifting is improved. The traverse board 10 is provided with a second lifting position sensor 17 (which can be a plurality of lifting position sensors arranged in sequence), and the second lifting frame 12 is provided with a second lifting trigger piece 18 for triggering the second lifting position sensor 17, so as to detect the lifting height of the second lifting frame 12 and avoid the excessive lifting position.

Referring to fig. 2 to 4, as another embodiment of the free nucleic acid extractor provided by the present application, the traverse driving mechanism 5 includes a traverse driving pulley 501 rotatably mounted on the top base 4, a traverse driven pulley 502 rotatably mounted on the top base 4, a traverse transmission belt 503 connected between the traverse driving pulley 501 and the traverse driven pulley 502, and a traverse motor 504 mounted on the top base 4 and configured to drive the traverse driving pulley 501 to rotate; the traverse plate 10 is connected to a traverse belt 503. The traverse motor 504 drives the traverse driving pulley 501 to rotate, and further drives the traverse driving belt 503 to move by matching with the traverse driven pulley 502, and the traverse driving belt 503 drives the traverse plate 10 to move when moving, so as to complete the transverse movement of the traverse plate 10.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided in the present application, a traverse position sensor 19 is disposed on the top seat 4, and a traverse trigger sheet 20 for triggering the traverse position sensor 19 is disposed on the traverse plate 10, so as to detect a moving position of the traverse plate 10 and avoid a transition of movement.

Referring to fig. 2-4, as another embodiment of the free nucleic acid extractor provided in the present application, the traverse driving mechanism 5 further includes a traverse guide 505 mounted on the top base 4, and a traverse slider 506 slidably mounted on the traverse guide 505; the traverse plate 10 is connected to the traverse slide 506. When the traverse plate 10 moves, the traverse plate tends to move only in the linear direction of the traverse guide 505, and is less likely to shift.

It should be understood that the above examples are merely examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present application shall be included in the protection scope of the claims of the present application.

Claims

1. The free nucleic acid extraction instrument is characterized by comprising a base, a heating plate arranged on the base, a plurality of extraction box bodies inserted on the heating plate, a top seat positioned above the extraction box bodies, a transverse moving plate slidably mounted on the base, a transverse moving driving mechanism used for driving the transverse moving plate to move, a first lifting frame slidably mounted on the transverse moving plate, a first lifting driving mechanism used for driving the first lifting frame to move, a second lifting frame slidably mounted on the transverse moving plate, and a second lifting driving mechanism used for driving the second lifting frame to move; the first lifting frame is provided with a plurality of sleeves, and the second lifting frame is provided with a plurality of magnetic rods opposite to the sleeves; the magnetic rods are in one-to-one correspondence with the sleeves, and the magnetic rods can be inserted into the sleeves.

2. The free nucleic acid extraction instrument of claim 1, wherein a vibration structure is further disposed in the base, and the vibration structure can drive the extraction box to vibrate.

3. The free nucleic acid extractor of claim 1, wherein the extraction cartridge has a first extraction tube slot and a plurality of second extraction tube slots; the first extraction pipe groove and the plurality of second extraction pipe grooves are arranged in sequence and at intervals along a preset direction.

4. The free nucleic acid extraction instrument of claim 1, wherein the heating plate is provided with a plurality of mounting slots, and the extraction cartridge is inserted into the mounting slots.

5. The free nucleic acid extractor of claim 1 wherein an end of the cannula distal from the first crane has a tip portion; the surface of the tip part is an arc surface.

6. The free nucleic acid extractor of claim 1, wherein the first elevation driving mechanism and the second elevation driving mechanism are disposed at opposite sides of the traverse plate, respectively.

7. The free nucleic acid extractor of any one of claims 1-6, wherein the first lift drive mechanism comprises a first drive pulley rotatably mounted to the traverse plate, a first driven pulley rotatably mounted to the traverse plate, a first drive belt connected between the first drive pulley and the first driven pulley, and a first lift motor mounted to the traverse plate for driving the first drive pulley to rotate; the first lifting frame is connected with the first transmission belt.

8. The free nucleic acid extractor of any of claims 1-6, wherein the second lift drive mechanism comprises a second drive pulley rotatably mounted to the traverse plate, a second driven pulley rotatably mounted to the traverse plate, a second drive belt connected between the second drive pulley and the second driven pulley, and a second lift motor mounted to the traverse plate for driving the second drive pulley to rotate.

9. The free nucleic acid extractor of any one of claims 1-6, wherein the traverse driving mechanism comprises a traverse driving pulley rotatably mounted to the top base, a traverse driven pulley rotatably mounted to the top base, a traverse driving belt connected between the traverse driving pulley and the traverse driven pulley, and a traverse motor mounted to the top base for driving the traverse driving pulley to rotate; the transverse moving plate is connected with the transverse moving transmission belt.

10. The apparatus as claimed in claim 9, wherein a traverse position sensor is provided on the top base, and a traverse trigger piece for triggering the traverse position sensor is provided on the traverse plate.