CN217549845U

CN217549845U - Biological sample preparation gun head structure of pipette

Info

Publication number: CN217549845U
Application number: CN202220421961.XU
Authority: CN
Inventors: 韦凤美
Original assignee: Lanzhou University
Current assignee: Lanzhou University
Priority date: 2022-03-01
Filing date: 2022-03-01
Publication date: 2022-10-11
Anticipated expiration: 2032-03-01

Abstract

The utility model discloses a biological system appearance rifle head structure of liquid-transfering gun, including interface portion, rifle head body and head end. The inner side wall of the connecting part is provided with a sealing convex ring, the outer side wall of the connecting part is provided with a bulge for clamping, and the bottom of the connecting part is connected with the gun head body; the side wall and the bottom end of the narrow-caliber end of the gun head body are provided with a plurality of through holes; the narrow-caliber end of the gun head body can be connected with a common gun head end, a grid type or a clamping type gun head end; and small through holes are formed in the outer wall and the bottom of the head end of the gun head. The interface portion can be connected with a pipette nozzle. The sampling gun head structure can be reused after sterilization. Use the utility model discloses a system appearance rifle head, the liquid work load of trading of reducible system appearance in-process reduces sample damage and pollution simultaneously.

Description

Biological system appearance rifle head structure of liquid-transfering gun

Technical Field

The utility model relates to a biological experiment equipment technical field especially relates to a biological system appearance rifle head structure of liquid-transfering gun.

Background

Most biological samples can be observed under a light mirror or an electron microscope only through the processes of fixing, dehydrating and the like. The sample is processed by using a full-automatic tissue processor, so that the device is convenient and labor-saving, but a plurality of small laboratories are not provided with the device. The EP tube is used for manually preparing samples, and the liquid adding and changing are time-consuming and labor-consuming, and the samples are easy to damage and cause pollution. The existing liquid-transfering gun biological sample-preparing gun head is used for preparing samples, the samples can still be damaged and polluted, and the liquid-transfering gun biological sample-preparing gun head can not be linked with a dyeing link. To sum up, aiming at the defects of the prior art, in order to reduce the workload of sample preparation and improve the working efficiency, a practical biological sample preparation gun head needs to be designed and manufactured.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing a biological sample preparation gun head structure of a liquid-transfering gun for solving the defects of the prior art.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a biological sample preparation gun head structure of a liquid-transfering gun comprises a connector part, a gun head body and a head end. The inner wall of the interface part is provided with a sealing convex ring, the outer wall of the interface part is provided with a clamping bulge, and the bottom of the interface part is connected with the gun head body; small through holes are uniformly distributed in the side wall of the lower part of the narrow-caliber end and the bottom end of the gun head body; the narrow-caliber end of the gun head body can be connected with a common gun head end, a grid type or a clamping type gun head end.

Further, the interface part can be hermetically connected with a pipette nozzle.

Furthermore, the outer wall of the interface part is provided with a bulge which can be inserted into the gun head box.

Furthermore, small through holes are uniformly distributed at the bottom end of the narrow-diameter end of the gun head body.

Furthermore, the narrow-caliber end of the gun head body can be connected with a common gun head end, a grid type or clamping groove type gun head end.

Furthermore, two crossed clamping plates are arranged at the bottom in the head end of the latticed gun head, and a formed grid can be used for vertically placing a copper mesh or a block-shaped sample.

Furthermore, two parallel clamping plates are arranged at the bottom in the head end of the clamping type gun head, and a slice sample can be placed in a vertical position.

Furthermore, the interface part, the gun head body, the common gun head end, the grid type gun head end and the clamping type gun head end are all made of high-temperature-resistant transparent or semitransparent polypropylene materials.

The beneficial effects of the utility model are that:

1. the utility model provides a biological system appearance rifle head reasonable in design, convenient operation of liquid-transfering gun for system appearance process can alleviate the work load of liquid changing, reduces sample damage and pollution simultaneously.

2. The utility model provides a biological system appearance rifle head of pipetting gun loads into rifle head box when using, through sterilization process, uses with the cooperation of standard pipetting gun, can accomplish a plurality of system appearance links such as fixed, rinsing, dehydration, dyeing, infiltration, embedding.

3. The utility model provides an interface part, rifle head body, ordinary type rifle head end, net type rifle head end and the type rifle head end that clamps of liquid-transfering gun biological system appearance rifle head end make by high temperature resistant transparent or translucent material, through still reuse clean, sterilization treatment after the use.

Drawings

Fig. 1 is a schematic structural view of a structure example 1 of a biological sample preparation gun head of a pipette gun provided by the present invention;

fig. 2 is an assembled perspective structure diagram of a biological sample preparation gun head structure of a pipette gun according to embodiment 2 of the present invention;

fig. 3 is an assembly and explosion structure diagram of a biological sample preparation gun head structure of a pipette gun according to embodiment 2 of the present invention;

fig. 4 is a schematic structural view of a head end of a mesh-type pipette head according to embodiment 3 of a structure of a biological sample pipette head of a pipette;

fig. 5 is a schematic view of a head end structure of a clamping type gun according to an embodiment 4 of a biological sample gun head structure of a pipette gun provided by the present invention;

in the figure: 1. an interface section; 2. a sealing convex ring; 3. an outer wall of the interface portion; 4. clamping the protrusion; 5. a gun head body; 6. a through hole; 7. a head end of a common gun head; 8. mesh openings; 9. a grid type or clamping type gun head end; 10. a parallel clamping plate; 11. a cross snap-gauge; 12. and (4) a copper net.

Detailed Description

The technical solution in the embodiments of the present invention is clearly and completely described below with reference to the accompanying drawings. The described embodiments are only some embodiments of the invention, and not all embodiments.

Example 1

For a biological sample which is not easy to damage, a sample is prepared by using the structure of the biological sample preparation gun head of the pipette shown in figure 1. The structure of the biological sample gun head of the pipette shown in figure 1 comprises a connector part 1, wherein a sealing convex ring 2 is arranged on the inner wall of the connector part 1 and can be connected with a pipette nozzle in a sealing way, a clamping bulge 4 is arranged on the outer wall 3 of the connector part 1, the bottom of the connector part 1 is connected with a gun head body 5, and through holes 6 are uniformly distributed on the side wall of the lower part of the narrow-diameter end and the bottom end of the gun head body 5. The sample is loaded into the gun head body 5, and then the gun head body 5 is hermetically connected with the interface part 1. Selecting a proper elastic suction nozzle of the pipette gun, inserting the elastic suction nozzle into the interface part 1 of the sample preparation gun head, pressing a liquid suction button of the pipette gun, then immersing the pipette gun into liquid, sucking the liquid to a reference line and staying for a certain time. Then, the liquid is pushed out slowly by pressing a liquid pushing button of the liquid transferring gun, and the biological sample preparation gun head is transferred to new liquid by the liquid transferring gun. And repeating the steps until the liquid changing step is finished. And (4) unloading the gun head body, and directly embedding or taking out the sample for further processing.

Example 2

For a large biological sample which is easy to damage, the structure of the biological sample preparation gun head of the pipette gun shown in fig. 2 and fig. 3 is used for preparing the sample. The structure of the biological sample gun head of the pipette shown in fig. 3 comprises a connector part 1, wherein a sealing convex ring 2 is arranged on the inner wall of the connector part 1 and can be connected with a pipette nozzle in a sealing way, a clamping bulge 4 is arranged on the outer wall 3 of the connector part 1, the bottom of the connector part 1 is connected with a gun head body 5, and through holes 6 are uniformly distributed at the bottom end of the narrow-diameter end of the gun head body 5. The narrow-caliber end of the gun head body 5 is connected with a common gun head end 7. The periphery and the bottom of the head end 7 of the common gun head are uniformly distributed with small meshes 8. A large biological sample which is easy to damage is loaded into the head end 7 of a common type gun head and then is connected with the gun head body 5 and the interface part 1. Selecting a proper elastic suction nozzle of the pipette gun to be inserted into the interface part 1 of the sample preparation gun head, pressing a liquid suction button of the pipette gun to immerse the pipette gun into liquid, sucking the liquid to a reference line and staying for a certain time. Then, the liquid is slowly pushed out by pressing a liquid pushing button of the liquid transferring gun, and the biological sample preparation gun head is transferred to new liquid by using the liquid transferring gun. And repeating the steps until the liquid replacement is finished. The head end 7 of the common gun head is detached, and the sample is directly embedded or taken out for further processing.

Example 3

For the small biological samples which are easy to damage, the structure of the biological sample preparation gun head of the pipette gun shown in figure 4 is used for sample preparation. Referring to fig. 4, a pipetting gun biological sample gun head structure comprises a connector 1, wherein a sealing convex ring 2 is arranged on the inner wall of the connector 1 and is hermetically connected with the pipetting gun, a clamping bulge 4 is arranged on the outer wall 3 of the connector 1, a gun head body 5 is connected with the bottom of the connector 1, through holes 6 are uniformly distributed at the bottom end of the narrow-diameter end of the gun head body 5, and the connector can be connected with a grid type gun head end 9. And (3) filling different samples into the grid type gun head end 9 in a grid mode, and then connecting the grid type gun head end 9 with the gun head body 5 in a sealing mode. Selecting a proper elastic suction nozzle of the pipetting gun, slowly inserting the elastic suction nozzle into the interface part 1 of the sample preparation gun head, hermetically connecting, pressing a liquid suction button of the pipetting gun, immersing the pipetting gun into liquid, sucking the liquid until at least the head end 9 of the grid type gun head is immersed, staying for a certain time, pressing a liquid push button of the pipetting gun to slowly push out the liquid, and transferring the biological sample preparation gun head to new liquid by using the pipetting gun. And repeating the steps until the liquid replacement is finished. And (3) unloading the head end 9 of the grid type gun head, and taking out the sample for further treatment or direct embedding. The circumference outer wall and the bottom of net type rifle head end 9 all are equipped with mesh 8. The bottom of the head end 9 of the grid-type gun head is provided with two criss-cross clamping plates 11, the bottom of the mesh is divided into 4 areas, and 4 different samples or copper nets 12 can be loaded in the areas.

Example 4

For the thin slice biological samples, the pipette tip structure shown in fig. 5 was used for sampling. Referring to fig. 5, the biological sample preparation gun head structure of the pipette comprises a connector part 1, wherein a sealing convex ring 2 is arranged on the inner wall of the connector part 1 and can be hermetically connected with the pipette, a clamping bulge 4 is arranged on the outer wall 3 of the connector part 1, the bottom of the connector part 1 is connected with a gun head body 5, through holes 6 are uniformly distributed on the side wall and the bottom end of the lower part of the narrow-diameter end of the gun head body 5, and the narrow-diameter end of the gun head body 5 is connected with a clamping gun head end 9. And clamping the sheet sample in the clamping type gun head end 9, and then hermetically connecting the clamping type gun head end 9 with the gun head body 5. Selecting a proper elastic suction nozzle of the pipette gun, slowly inserting the elastic suction nozzle into the interface part 1 of the sample preparation gun head, hermetically connecting, pressing a liquid suction button of the pipette gun, immersing the pipette gun in liquid for sucking until at least the head end 9 of the clamped gun head is immersed, staying for a certain time, then pressing a liquid push button of the pipette gun to slowly push out the liquid, and transferring the biological sample preparation gun head to new liquid by using the pipette gun. And repeating the steps until the liquid is changed. And taking down the head end 9 of the clamping type gun head, and taking out the sample for further processing or directly embedding. And meshes 8 are arranged on the circumferential outer wall and the bottom of the clamping type gun head end 9. The bottom of the head end 9 of the gun head is provided with two parallel clamping plates 10, and a thin sheet sample or a copper mesh 12 can be placed in a vertical position.

The working principle of the embodiments 1-4 is as follows: putting a sample into a sample preparation tube, selecting a proper elastic suction nozzle of a pipette gun, inserting the elastic suction nozzle into the interface part 1 of the tip of the sample preparation gun, keeping the airtight connection, pressing a liquid suction button of the pipette gun, slowly immersing the pipette gun into liquid to suck the liquid to a reference line, staying for a certain time, pressing a liquid push button of the pipette gun to slowly push out the liquid, and transferring the tip of the pipette gun to new liquid by using the pipette gun. And repeating the steps until the liquid changing step is completed.

The utility model relates to a interface part 1, rifle head body 5, ordinary type rifle head end 7 and net type or clamp type rifle head end 9 of pipetting gun biological system appearance rifle head are made by high temperature resistant transparent or translucent material, but still reuse through washing, sterilization treatment after the use.

The above, only be the preferred embodiment of the present invention, nevertheless the utility model discloses a protection scope is not limited to this, and any technical personnel who is familiar with this technical field is in the technical scope of the utility model, according to the utility model discloses a technical scheme and utility model design replace or change in addition equally, all should be covered within the protection scope of the utility model.

In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.

Claims

1. A pipette head structure for biological sample preparation comprises a connector part (1), and is characterized in that a sealing convex ring (2) is arranged on the inner wall of the connector part (1), a clamping bulge (4) is arranged on the outer wall (3), and the bottom of the connector part is connected with a pipette head body (5); small through holes (6) are uniformly distributed on the side wall and the bottom end of the narrow-diameter end of the gun head body (5); the narrow-caliber end of the gun head body (5) can be further connected with a common gun head end (7) and a grid type or clamping type gun head end (9); and meshes (8) are arranged on the circumferential outer walls and the bottoms of the common type gun head end (7) and the grid type or clamping type gun head end (9).

2. The pipette gun head structure for biological samples according to claim 1, wherein the interface part (1) is connectable to a pipette nozzle.

3. The pipette gun head structure for biological samples according to claim 1, wherein the outer wall (3) of the interface part (1) is provided with a clamping projection (4) which can clamp the biological sample gun head to the gun head box.

4. The pipette gun head structure for biological samples according to claim 1, wherein the narrow-diameter end of the gun head body (5) can be connected with a common gun head end (7), a grid type or a clamping type gun head end (9).

5. The pipette gun head structure for biological samples according to claim 4, wherein the narrow-diameter end of the gun head body (5) is connected with a common gun head end (7) and a grid or clamping type gun head end (9) in a knob shape or a hook shape.

6. The pipette gun head structure for biological samples according to claim 4, wherein the bottom of the head end (9) of the clamping gun head is provided with two parallel clamping plates (10) for vertically placing thin samples or materials.

7. The pipette tip structure according to claim 4, wherein the grid-type pipette tip head (9) has two criss-cross clamping plates (11) at the bottom to form four small holes for vertically placing copper mesh (12) or block-shaped samples.

8. The pipette gun head structure for biological samples according to claim 1, wherein the interface part (1), the gun head body (5), the common gun head end (7) and the grid/clamping type gun head end (9) are all made of high-temperature-resistant transparent or semitransparent polypropylene materials.