CN217060237U - Platelet antibody quantum dot micro-column gel analysis and interpretation instrument - Google Patents

Abstract

The utility model discloses a platelet antibody quantum dot microcolumn gel analysis interpretation instrument, which comprises a light source module, a sliding table module, a receiver module and a bottom plate, wherein the light source module comprises a support frame, a clamping groove, a fixed seat and a light source, the support frame is fixedly connected with the top of the bottom plate, the clamping groove is a cavity structure and is communicated up and down, the fixed seat is positioned at one side of the clamping groove, the light source is fixedly connected in the fixed seat, the sliding table module comprises a motor, a sliding rail, a sliding block, a screw rod, a clamping support and a U-shaped bracket, the cross section of the clamping support is of an inverted T-shaped structure, the upper part of the clamping support extends into the clamping groove, the receiver module comprises an optical signal acquisition circuit board, a circuit board fixed frame and an optical filter, the circuit board fixed frame is fixedly connected at the other side of the clamping groove, the optical filter is positioned in the circuit board fixed frame, the optical signal acquisition circuit board is fixedly connected at one side of the circuit board fixed frame, the utility model discloses can reduce clinical operation step, practice thrift check-out time, help clinical disease diagnosis.

Description

Platelet antibody quantum dot microcolumn gel analysis and interpretation instrument

Technical Field

The utility model belongs to the external blood detection technology specifically relates to a platelet antibody quantum dot microcolumn gel analysis interpretation appearance.

Background

Platelet antibody can cause the patients to have platelet immune injury, and generate immune thrombocytopenia, such as ineffective platelet transfusion, purpura after transfusion, autoimmune thrombocytopenia, neonatal thrombocytopenia, and the like, and platelet antibody detection is helpful for clinically diagnosing the causes of thrombocytopenia of patients. For the patients with platelet antibody positive, the infusion-compatible platelets can effectively reduce the risk of platelet infusion invalidation of the patients and improve the infusion curative effect.

At present, methods for detecting platelet antibodies mainly include solid-phase enzyme-linked immunosorbent assay (ELISA), flow cytometry, thromboelastography detection, and microcolumn gel. Wherein, both the solid-phase enzyme-linked immunosorbent assay (ELISA) method and the flow cytometry method have extremely high sensitivity and specificity, but the operation process is complicated, and the stability of the detection result is poor; although the monoclonal antibody solid phase platelet antibody (MAIPA) detection technology is a platelet antibody analysis standard method recommended in international uplink industry, the test procedure is complicated, the detection time needs more than 8 hours, and the method is only suitable for detecting a few samples in a laboratory, has high cost and influences the clinical popularization and use of the method; the microcolumn gel method is popular due to its convenience and simplicity, but because the platelets themselves have the characteristic of easy activation and self-aggregation, the interpretation of the final result is often influenced, and false positive is caused.

SUMMERY OF THE UTILITY MODEL

For overcoming the defects of the prior art, the utility model provides a platelet antibody quantum dot microcolumn gel analysis interpretoscope, which can reduce the clinical operation steps, save the detection time and is helpful for the clinical disease diagnosis.

In order to realize the purpose, the utility model discloses a technical scheme is:

the platelet antibody quantum dot micro-column gel analysis interpretoscope comprises a light source module, a sliding table module, a receiver module and a bottom plate, wherein the light source module and the sliding table module are both fixedly connected to the top surface of a floor, the receiver module is fixedly connected to one side of the light source module, the light source module comprises a support frame, a clamping groove, a fixed seat and a light source, the support frame is fixedly connected to the top of the bottom plate, the clamping groove is of a cavity structure, is communicated up and down and is fixedly connected to the top of the support frame, the fixed seat is positioned on one side of the clamping groove and is fixedly connected to the top of the support frame, the light source is fixedly connected into the fixed seat, the sliding table module comprises a motor, a sliding rail, a sliding block, a lead screw, a clamping support and a U-shaped support, the motor is fixedly connected to one side of the U-shaped support, the output end of the motor is fixedly connected with the lead screw, and the lead screw is positioned between the U-shaped supports, and rotate with U type support and be connected, slide rail fixed connection is in the bottom of U type support, the lead screw run through the slider and with slider threaded connection, the bottom and the slide rail sliding connection of slider, the cross section that the card held in the palm is "T" type structure, and its upper portion stretches into in the draw-in groove, the receiver module includes optical signal collection circuit board, circuit board fixed frame, light filter, circuit board fixed frame fixed connection is in the opposite side of draw-in groove, the light filter is located circuit board fixed frame, optical signal collection circuit board fixed connection is in one side of circuit board fixed frame.

Preferably, two side surfaces of the clamping groove are respectively provided with a through hole, the two through holes correspond to each other, and the emitting position of the light source corresponds to the through holes.

Preferably, the filter is located at one side of the through hole and covers the through hole.

Preferably, the light source adopts an ultraviolet LED collimation light source, the wavelength is 365nm, and the diameter of a light spot is 1 mm.

Preferably, the top of the card holder is provided with a strip-shaped groove.

Preferably, the card holds in the palm one side and still is equipped with a stop gear, stop gear structure includes photoelectric displacement switch, switch seat, response arm, switch seat fixed connection is on the bottom plate, and a photoelectric displacement switch is installed to its one side, the upper end and the card of response arm hold in the palm fixed connection, be equipped with a breach on the switch seat, the lower extreme and the breach of response arm correspond, and the overall dimension of breach is greater than the overall dimension of response arm lower extreme.

The utility model has the advantages that:

the utility model adopts the technical principle of water-based gel microcolumn immunoassay, so that the antigen-antibody compound is separated from the unreacted plasma components with high efficiency, the repeated washing step in the test process is avoided, the clinical operation steps are reduced, and the detection time is saved; and quantum dot immune labeling anti-human globulin is adopted to realize labeling analysis of the platelet antigen-antibody complex, compared with the conventional solid phase immune analysis method, the sensitivity is higher, the detection rate of platelet antibodies of patients with clinical platelet immune diseases is higher, and the diagnosis of clinical diseases is facilitated.

Drawings

Fig. 1 is a schematic structural view of the present invention;

fig. 2 is a top view of the present invention;

FIG. 3 is a schematic view of a card slot;

FIG. 4 is a schematic view of a card holder;

FIG. 5 is a schematic structural diagram of an embodiment;

FIG. 6 is a schematic view of the detection light path of the platelet antibody quantum dot microcolumn gel analysis interpretoscope;

the reference symbols shown in the figures are: 1-bottom plate, 2-support frame, 3-clamping groove, 4-fixing seat, 5-light source, 6-motor, 7-sliding rail, 8-sliding block, 9-lead screw, 10-clamping support, 11-U-shaped support, 12-optical signal acquisition circuit board, 13-circuit board fixing frame, 14-optical filter, 15-through hole, 16-groove, 17-photoelectric displacement switch, 18-switch seat, 19-notch, 20-induction arm and 21-microcolumn gel card.

Detailed Description

The following describes the present design in detail with reference to the accompanying drawings.

As shown in fig. 1-5, the platelet antibody quantum dot microcolumn gel analysis and interpretation instrument comprises a light source 5 module, a sliding table module, a receiver module, a bottom plate 1, wherein the light source 5 module is used for emitting light beams, the receiver module is used for receiving and analyzing the light beams, the sliding table module is used for fixing and stably moving a microcolumn gel card 21, the light source 5 module and the sliding table module are both fixedly connected to the top surface of the floor, the receiver module is fixedly connected to one side of the light source 5 module, the light source 5 module comprises a support frame 2, a clamping groove 3, a fixed seat 4 and a light source 5, the support frame 2 is fixedly connected to the top of the bottom plate 1, the clamping groove 3 is a cavity structure, the clamping groove is vertically communicated and fixedly connected to the top of the support frame 2, two side surfaces of the clamping groove 3 are respectively provided with a through hole 15, the two through holes 15 correspond to each other, and the emitting position of the light source 5 corresponds to the through hole 15, fixing base 4 is located one side of draw-in groove 3, and fixed connection is in the top of support frame 2, and light source 5 fixed connection adopts ultraviolet LED collimated light source 5 in fixing base 4, wavelength 365nm, facula diameter 1 mm. The sliding table module structure comprises a motor 6, a sliding rail 7, a sliding block 8, a lead screw 9, a clamping support 10 and a U-shaped support 11, the motor 6 is fixedly connected to one side of the U-shaped support 11, an output end of the motor is fixedly connected with the lead screw 9, the lead screw 9 is located between the U-shaped supports 11 and is rotatably connected with the U-shaped supports 11, the sliding rail 7 is fixedly connected to the bottom of the U-shaped supports 11, the lead screw 9 penetrates through the sliding block 8 and is in threaded connection with the sliding block 8, the bottom of the sliding block 8 is in sliding connection with the sliding rail 7, the cross section of the clamping support 10 is of an inverted T-shaped structure, the upper portion of the clamping support extends into a clamping groove 3, a strip-shaped groove 16 is arranged at the top of the clamping support 10, the groove 16 is used for clamping the microcolumn gel card 21, the whole sliding table module can drive the microcolumn gel card 21 to reciprocate, a limiting mechanism is further arranged on one side of the clamping support 10, and the limiting mechanism structure comprises a photoelectric displacement switch 17 and a switch seat 18, The sensor module comprises a sensing arm 20, a switch seat 18 is fixedly connected on the bottom plate 1, a photoelectric displacement switch 17 is arranged on one side of the sensing arm, the upper end of the sensing arm 20 is fixedly connected with the clamping support 10, a notch 19 is arranged on the switch seat 18, the lower end of the sensing arm 20 corresponds to the notch 19, the outline dimension of the notch 19 is larger than the outline dimension of the lower end of the sensing arm 20, the photoelectric displacement switch 17 is a limit turning point due to a limit mechanism, when the sensing arm 20 moves to the end, the photoelectric displacement switch 17 transmits a signal to enable the motor 6 to rotate reversely, the sensing arm 20 moves back to an initial position under the driving of the sliding block 8, the receiver module comprises an optical signal acquisition circuit board 12, a circuit board fixing frame 13 and an optical filter 14, the circuit board fixing frame 13 is fixedly connected to the other side of the clamping groove 3, the optical filter 14 is positioned in the circuit board fixing frame 13, and the optical filter 14 is positioned on one side of the through hole 15, and covers the through hole 15, and the optical signal collecting circuit board 12 is fixedly connected to one side of the circuit board fixing frame 13.

Examples

The utility model discloses the detection principle of using as follows:

after the platelet antibody reacts with a patient sample, a platelet immune complex is obtained through aqueous gel separation, and then the platelet immune complex reacts with quantum dot labeled anti-human globulin, and a particle antigen-antibody complex is formed through positive reaction and carries a quantum dot label. Then, the particle antigen-antibody complex with larger specific gravity in the positive reaction is distributed at the lower edge of the detection window of the micro-column card in the aqueous gel, and the platelet with smaller specific gravity in the negative reaction is distributed in the micro-column at the upper layer of the micro-column card. The instrument adopts 365nm ultraviolet light to excite the lower edge of the micro-column card, the quantum dots emit light with a wavelength of 610nm under the excitation of the ultraviolet light, the instrument detects the lower edge of the window of the micro-column card through the optical signal acquisition circuit board 12, the detected optical signal of the quantum dots is a positive detection result, and otherwise, the detected optical signal is a negative result.

When the micro-column gel card 21 is actually used, the optical signal acquisition circuit board 12 is connected with the PC end and is used in a matching way, the micro-column gel card 21 added with a reagent and a sample and centrifuged is placed in the clamping groove 3, the upper part of the clamping groove 3 is covered with a cover to form a darkroom, the height of the lower edge of a window of a detection window of the micro-column gel card 21 is consistent with the height of a light spot (namely a through hole 15) of the light source 5, under the control of a microprocessor, the motor 6 rotates to drive the sliding block 8 and the clamping groove 3 to horizontally move, the lower edges of six detection windows of the micro-column gel card 21 sequentially pass through the light spot of the light source 5, the window with quantum dots emits light with the wavelength of 610nm through the excitation light of 365nm, stray light is filtered out through the 610nm optical filter 14 and is received by the optical signal acquisition circuit board 12, the signal is transmitted to the receiving end of the signal amplifier by the output end of the optical signal acquisition circuit board 12, and the signal is transmitted to the receiving end of the A/D conversion circuit by the output end after shaping and amplification, the output end of the A/D conversion circuit transmits the signal to the microprocessor, the microprocessor transmits the signal to the PC, a real-time energy curve is drawn, and finally, a detection curve detection light path can be observed in real time through a PC terminal as shown in figure 6.

Claims (6)

1. The platelet antibody quantum dot micro-column gel analysis interpretation instrument is characterized by comprising a light source module, a sliding table module, a receiver module and a bottom plate, wherein the light source module and the sliding table module are both fixedly connected to the top surface of a floor, the receiver module is fixedly connected to one side of the light source module, the light source module structure comprises a support frame, a clamping groove, a fixed seat and a light source, the support frame is fixedly connected to the top of the bottom plate, the clamping groove is of a cavity structure, is communicated from top to bottom and is fixedly connected to the top of the support frame, the fixed seat is positioned on one side of the clamping groove and is fixedly connected to the top of the support frame, the light source is fixedly connected in the fixed seat, the sliding table module structure comprises a motor, a sliding rail, a sliding block, a lead screw, a clamping support and a U-shaped bracket, the motor is fixedly connected to one side of the U-shaped bracket, and the output end of the motor is fixedly connected with the lead screw, the lead screw is located between the U type support, and rotates with U type support to be connected, slide rail fixed connection is in the bottom of U type support, the lead screw run through the slider and with slider threaded connection, the bottom and the slide rail sliding connection of slider, the cross section that the card held in the palm is for falling "T" type structure, and its upper portion stretches into in the draw-in groove, the receiver module includes optical signal acquisition circuit board, circuit board fixed frame, light filter, circuit board fixed frame fixed connection is in the opposite side of draw-in groove, the light filter is located circuit board fixed frame, optical signal acquisition circuit board fixed connection is in one side of circuit board fixed frame.

2. The platelet antibody quantum dot microcolumn gel analysis and interpretation instrument according to claim 1, wherein two side surfaces of the card slot are respectively provided with a through hole, two of the through holes correspond to each other, and an emission position of the light source corresponds to the through hole.

3. The platelet antibody quantum dot microcolumn gel analysis reader of claim 1, wherein the filter is located at one side of the through hole and covers the through hole.

4. The platelet antibody quantum dot microcolumn gel analysis and interpretation instrument according to claim 1, wherein the light source is an ultraviolet LED collimated light source, the wavelength is 365nm, and the diameter of a light spot is 1 mm.

5. The platelet antibody quantum dot microcolumn gel analysis and interpretation instrument according to claim 1, wherein a long-strip-shaped groove is formed in the top of the card holder.

6. The platelet antibody quantum dot microcolumn gel analysis and interpretation instrument according to claim 1, wherein a limit mechanism is further disposed on one side of the card holder, the limit mechanism comprises a photoelectric displacement switch, a switch seat and an induction arm, the switch seat is fixedly connected to the bottom plate, the photoelectric displacement switch is mounted on one side of the switch seat, the upper end of the induction arm is fixedly connected with the card holder, a notch is disposed on the switch seat, the lower end of the induction arm corresponds to the notch, and the outer dimension of the notch is larger than the outer dimension of the lower end of the induction arm.

Images