CN217038070U - Culture apparatus of nursery stock arbuscular mycorrhizal fungi - Google Patents
Culture apparatus of nursery stock arbuscular mycorrhizal fungi Download PDFInfo
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- CN217038070U CN217038070U CN202220649612.3U CN202220649612U CN217038070U CN 217038070 U CN217038070 U CN 217038070U CN 202220649612 U CN202220649612 U CN 202220649612U CN 217038070 U CN217038070 U CN 217038070U
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Abstract
The application provides a culture apparatus of nursery stock arbuscular mycorrhizal fungi includes: a seedling planting tank, a mycorrhizal fungi culture tank and a nutrient medium tank; the seedling planting tank, the mycorrhizal fungi culture tank and the nutrient medium tank are sequentially arranged in a stacked mode from top to bottom. The bottom of the seedling planting tank and the bottom of the mycorrhizal fungi culture tank are provided with air-permeable components which are paved on the whole bottom; the air-permeable assembly includes: the screen cloth and the transfer line, the screen cloth is connected with the transfer line. The seedling planting groove is used for planting seedling seedlings or seeds; the mycorrhizal fungi culture tank is used for symbiotic culture of arbuscular mycorrhizal fungi and nursery stocks; the nutrition medium groove is used for providing nutrition medium for the growth of the nursery stock and the arbuscular mycorrhizal fungi. The layer which is arranged separately is used for culturing the arbuscular mycorrhizal fungi, so that the arbuscular mycorrhizal fungi and the root system of the seedling have a proper symbiotic space. By means of layered arrangement, the costs of the arbuscular mycorrhizal fungi matrix can be reduced, and the effect of promoting the growth of seedling root systems is achieved.
Description
Technical Field
The application relates to the technical field of microbial cultivation, in particular to a cultivation device for arbuscular mycorrhizal fungi of nursery stock.
Background
Mycorrhiza is a symbiont formed by mycorrhizal fungi in soil and root systems of higher plants, and is one of the most common symbiosis phenomena in nature. Mycorrhizal fungi are divided into ectomycorrhizal fungi and endophytic mycorrhizal fungi, which are also called vesicular arbuscular mycorrhizal fungi and are called arbuscular mycorrhizal fungi for short.
The research at home and abroad mainly focuses on arbuscular mycorrhizal fungi, and the arbuscular mycorrhizal fungi can form mycorrhizal symbionts with most crops, woody plants and herbaceous plants. Research shows that the arbuscular mycorrhizal fungi can improve the stress resistance of plants, such as drought resistance, heat resistance, waterlogging resistance, salt resistance, heavy metal ion resistance, disease resistance, cold resistance, acid resistance, insect resistance and the like. The arbuscular mycorrhizal fungi can not be propagated in vitro and can only be propagated after symbiosis with the root system of the living plant.
In traditional agriculture, people pay attention to the application of chemical fertilizers and pesticides, but neglect the biological action of beneficial microorganisms such as arbuscular mycorrhizal fungi and the like. The related art mainly focuses on how to culture the arbuscular mycorrhizal fungi to interact with plants, and does not focus on a device for culturing the arbuscular mycorrhizal fungi. The culture of arbuscular mycorrhizal fungi depends not only on the culture medium but also on the culture apparatus environment. If the environment of the culture device is not suitable for culturing the arbuscular mycorrhizal fungi, the arbuscular mycorrhizal fungi can also cause poor growth and cannot achieve good symbiotic effect with plants, so that the arbuscular mycorrhizal fungi have long propagation period and high cost.
SUMMERY OF THE UTILITY MODEL
The application provides a culture apparatus of nursery stock arbuscular mycorrhizal fungi to solve the problem that the preparation time of culture arbuscular mycorrhizal fungi matrix is long, with high costs.
A culture apparatus of arbuscular mycorrhizal fungi of nursery stock, comprising: a seedling planting tank, a mycorrhizal fungi culture tank and a nutrient medium tank; the seedling planting tank, the mycorrhizal fungi culture tank and the nutrient medium tank are sequentially arranged in a laminated manner from top to bottom; openings are formed in the tops of the seedling planting groove and the mycorrhizal fungi culture groove, side walls are arranged on the peripheries of the seedling planting groove and the mycorrhizal fungi culture groove, and air-permeable components are arranged at the bottoms of the seedling planting groove and the mycorrhizal fungi culture groove and are fully paved at the bottoms of the seedling planting groove and the mycorrhizal fungi culture groove; the air-permeable assembly includes: a screen and a transfusion tube; the screen is connected with a liquid conveying pipe, and the screen is connected with the side walls of the seedling planting groove and the mycorrhizal fungi culture groove; the infusion tube transversely spans the bottoms of the seedling planting groove and the mycorrhizal fungi culture groove, two ends of the infusion tube are respectively connected with different side walls of the seedling planting groove and the mycorrhizal fungi culture groove, and a plurality of holes are formed in the infusion tube.
The culture device is generally arranged in a cuboid shape and is divided into three layers, so that the culture device can be conveniently packaged and transported later. The upper layer is a seedling planting groove used for planting seedling seedlings or seeds. The middle layer is a mycorrhizal fungi culture tank and is used for symbiotic culture of the arbuscular mycorrhizal fungi and the nursery stocks, and the independently arranged layer can configure corresponding environment according to the growth of the arbuscular mycorrhizal fungi without worrying about influencing the growth of the nursery stocks on the upper layer. The lower layer is a nutrition substrate groove used for providing nutrition substrates for the growth of the nursery stocks and the arbuscular mycorrhizal fungi.
The bottom of the seedling planting tank and the bottom of the mycorrhizal fungi culture tank are provided with ventilating components for the root system of the seedling to permeate, so that the arbuscular mycorrhizal fungi can be cultured and propagated after symbiosis with the root system of the seedling. The air-permeable assembly includes: screen cloth and transfer line, screen cloth cover the seedling planting groove and mycorrhizal fungi culture tank bottom can provide fine gas permeability, make things convenient for the root system of nursery stock to permeate out. The transfusion tube can be conveniently watered or used for conveying nutrient solution or liquid medicine, and can play a role in bearing similar to a cross beam.
Optionally, the culture device further comprises a liquid conveying tank, the liquid conveying tank is arranged on the outer side walls of the seedling planting tank and the mycorrhizal fungi culture tank, and the liquid conveying tank is connected with the liquid conveying pipe.
The infusion liquid tank is used for conveying water or nutrient solution into the infusion tube, and can be arranged at the same side for conveniently and uniformly irrigating.
Optionally, the number of the infusion tubes is two, one of the infusion tubes transversely passes through the bottom central axis, and the other infusion tube longitudinally passes through the bottom central axis to form a cross structure.
The bottoms of the seedling planting tank and the mycorrhizal fungi culture tank are equivalent to two crossed cross beams, so that a better bearing effect can be achieved; meanwhile, the infusion tubes which are arranged in a cross way can irrigate more uniformly.
Optionally, the number of the infusion tubes is two, and the two infusion tubes are respectively arranged along the diagonal lines of the bottom to form a cross structure.
The bottoms of the seedling planting tank and the mycorrhizal fungi culture tank are equivalent to two cross beams with crossed diagonals, so that a better bearing effect can be achieved; meanwhile, the infusion tubes which are arranged in a diagonal line crossing way can irrigate more uniformly.
Optionally, the side length of the mesh holes of the screen is 2-3mm, and the diameter of the mesh lines is less than or equal to 1 mm.
The arrangement is to prevent the culture substrate or soil from leaking from the mesh holes of the screen, if the size of the mesh holes is too large, the culture substrate or soil will leak from the mesh holes, causing the loss of the culture substrate or soil, and increasing the culture cost; if the size of the grid holes is too small, the air permeability is poor, the penetration of the seedling root system is influenced, and the culture period and effect are increased. Meanwhile, the diameter of the grid line is less than or equal to 1mm, so that the area of the grid hole is increased to the maximum extent, the air permeability is increased, and the bearing capacity is also ensured.
Optionally, the screen mesh is a PVC mesh or a wire mesh with an anti-corrosion coating.
Optionally, the infusion tube is a PVC tube or a steel tube with an anti-corrosion coating.
PVC (Polyvinyl chloride) is the most popular plastic in the world, and the application is very wide, and the material can be manufactured by an integral forming method with lower cost. The wire netting and the steel pipe with the anti-corrosion coating can obtain a better bearing effect.
Optionally, the height of the mycorrhizal fungi culture tank is 10-15 cm.
The arrangement is to ensure that the arbuscular mycorrhizal fungi and the root system of the seedling have proper symbiotic space. The arbuscular mycorrhizal fungi substrate is expensive, and the height of the mycorrhizal fungi culture tank is too high, so that the cost of using the arbuscular mycorrhizal fungi substrate is increased; the height of the mycorrhizal fungi culture tank is too low, and the produced symbiotic effect is reduced.
The application provides a culture apparatus of nursery stock arbuscular mycorrhizal fungi includes: a seedling planting tank, a mycorrhizal fungi culture tank and a nutrient medium tank; the seedling planting tank, the mycorrhizal fungi culture tank and the nutrient medium tank are sequentially arranged in a stacked mode from top to bottom. The bottom parts of the seedling planting tank and the mycorrhizal fungi culture tank are provided with air-permeable components which are paved on the whole bottom part; the air-permeable assembly includes: the screen cloth and the transfer line, the screen cloth is connected with the transfer line. The seedling planting groove is used for planting seedling seedlings or seeds; the mycorrhizal fungi culture tank is used for symbiotic culture of arbuscular mycorrhizal fungi and nursery stocks; the nutrition medium groove is used for providing nutrition medium for the growth of the nursery stock and the arbuscular mycorrhizal fungi. The layer which is arranged separately is used for culturing the arbuscular mycorrhizal fungi, so that the arbuscular mycorrhizal fungi and the root system of the seedling have a proper symbiotic space. By means of layered arrangement, the costs of the arbuscular mycorrhizal fungi matrix can be reduced, and the effect of promoting the growth of seedling root systems is achieved.
Drawings
In order to more clearly explain the technical solution of the present application, the drawings needed to be used in the embodiments are briefly described below, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic view of the structure of the culture apparatus;
FIG. 2 is a front view of the culture apparatus;
FIG. 3 is a schematic view of the structure of a first embodiment of the air permeable assembly;
fig. 4 is a schematic structural view of a second embodiment of the air-permeable assembly.
Illustration of the drawings:
wherein, the culture medium comprises a nutrient medium groove 1, a mycorrhizal fungi culture groove 2, a seedling planting groove 3, a ventilation component 4, a transfusion groove 5, a screen 41 and a transfusion tube 42.
Detailed Description
Reference will now be made in detail to embodiments, examples of which are illustrated in the accompanying drawings. When the following description refers to the accompanying drawings, like numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following examples do not represent all embodiments consistent with the present application. But merely as exemplifications of systems and methods consistent with certain aspects of the application, as recited in the claims.
The present application provides a device for culturing arbuscular mycorrhizal fungi of nursery stock, as shown in fig. 1, includes: a seedling planting groove 3, a mycorrhizal fungi culture groove 2 and a nutrient medium groove 1; the seedling planting tank 3, the mycorrhizal fungi culture tank 2 and the nutrient medium tank 1 are sequentially arranged in a stacked manner from top to bottom; openings are formed in the tops of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2, side walls are arranged on the peripheries of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2, air-permeable components 4 are arranged at the bottoms of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2, and the air-permeable components 4 are paved on the bottoms of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2; the air-permeable assembly 4 comprises: a screen 41 and a transfusion tube 42; the screen 41 is connected with a liquid conveying pipe 42, and the screen 41 is connected with the side walls of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2; the infusion tube 42 stretches across the bottoms of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2, two ends of the infusion tube are respectively connected with different side walls of the seedling planting groove 3 and the mycorrhizal fungi culture groove 2, and a plurality of holes are formed in the infusion tube 42.
The culture device is generally arranged in a cuboid shape and is divided into three layers, so that the culture device can be conveniently packaged and transported later. The upper layer is a seedling planting groove 3 for planting seedling seedlings or seeds, for example: and (5) sorghum seedlings. The middle layer is a mycorrhizal fungi culture tank 2 used for symbiotic culture of arbuscular mycorrhizal fungi and nursery stocks, such as: mycorrhizal fungi culture substrates of Glomus intraradicans (Glomus intraradics, BGCJX04B) and Glomus terreus (Glomus versiforme, BGC XJ 08F). The separately arranged layer can configure corresponding environment according to the growth of arbuscular mycorrhizal fungi without worrying about influencing the growth of upper nursery stocks. The lower layer is a nutrient medium groove 1 for providing nutrient medium for the growth of nursery stock and arbuscular mycorrhizal fungi, such as: nitrogen fertilizer or plant nutrient solution.
The bottom of the seedling planting tank 3 and the bottom of the mycorrhizal fungi culture tank 2 are provided with a ventilating component 4 for the root system of the seedling to permeate, so that the arbuscular mycorrhizal fungi can be cultured and propagated after symbiosis with the root system of the seedling. The air-permeable assembly 4 comprises: the screen 41 and the liquid conveying pipe 42, the screen 41 is paved to fill the seedling planting groove 3 and the bottom of the mycorrhizal fungi culture groove 2, so that good air permeability can be provided, and the root system of the seedling can conveniently permeate out. The infusion tube 42 can be conveniently watered, or used for conveying nutrient solution or liquid medicine, and can also play a role in bearing similar to a cross beam.
When in use, the nutrient medium groove 1 is firstly placed at a proper position and filled with the nutrient medium mixed with the nitrogen fertilizer; placing the mycorrhizal fungi culture tank 2 on the nutrient medium tank 1, and then filling the mycorrhizal fungi culture medium of saccyces intraradicis and sacculus terrestris into the mycorrhizal fungi culture tank 2; then the seedling planting groove 3 is placed on the mycorrhizal fungi culture groove 2, then culture soil is filled in the seedling planting groove 3, and finally the young sorghum seedlings are filled in the culture soil.
The root system of the sorghum seedling can permeate from the bottom of the seedling planting groove 3 to grow into the mycorrhizal fungi culture groove 2; in the mycorrhizal fungi culture tank 2, the root system of the sorghum seedling can be symbiotically cultured with the saccaromyces intraradicans and the saccaromyces terrestris; the root system can permeate from the bottom of the mycorrhizal fungi culture tank 2 to the nutrient medium tank 1 to absorb nutrient substances when the root system continues to grow. Generally, the seedlings can be packaged and sold after being cultured for three months, the seedling planting grooves 3 are directly packaged and boxed when being sold, and the mycorrhizal fungi culture groove 2 and the nutrient medium groove 1 can be reused.
In an exemplary embodiment, as shown in fig. 2, the cultivation apparatus further includes a liquid transfer tank 5, the liquid transfer tank 5 is disposed on the outer side walls of the seedling planting tank 3 and the mycorrhizal fungi cultivation tank 2, and the liquid transfer tank 5 is connected to the liquid transfer pipe 42.
The infusion liquid tank 5 is used for conveying water or nutrient solution into the infusion tube 42, and can be arranged on the same side to conveniently carry out uniform irrigation.
When in use, water is poured into the infusion tank 5, so that irrigation can be completed. Therefore, an automatic watering system is conveniently designed, and automatic watering can be completed only by introducing water into the liquid conveying tank 5 at regular time.
In an exemplary embodiment, as shown in fig. 3, the number of the infusion tubes 42 is two, wherein one of the infusion tubes 42 transversely passes through the bottom central axis position, and the other infusion tube 42 longitudinally passes through the bottom central axis position, forming a cross structure.
The bottoms of the seedling planting tank 3 and the mycorrhizal fungi culture tank 2 are equivalent to two crossed cross beams, so that a better bearing effect can be achieved; meanwhile, the infusion tubes 42 arranged in a cross way can irrigate more uniformly. The criss-cross infusion tubes 42 can be made by integral molding or can be connected by a criss-cross connector, the transverse and longitudinal infusion tubes 42 are communicated, and fluid can freely flow between the two infusion tubes 42.
In an exemplary embodiment, as shown in fig. 4, the number of the liquid conveying pipes 42 is two, and the two liquid conveying pipes 42 are arranged along diagonal positions of the bottoms of the seedling planting tank 3 and the mycorrhizal fungi culture tank 2 to form a cross structure.
The bottoms of the seedling planting tank 3 and the mycorrhizal fungi culture tank 2 are equivalent to two cross beams with crossed diagonals, so that a better bearing effect can be achieved; meanwhile, the infusion tubes 42 which are arranged in a diagonal cross way can irrigate more uniformly. The diagonally crossed infusion tubes 42 can be manufactured by an integral molding method, or can be connected by a four-way joint, the two diagonal infusion tubes 42 are communicated, and liquid can freely flow between the two infusion tubes 42.
In an exemplary embodiment, the screen 41 has a mesh opening side length of 2-3mm and a mesh line diameter of 1mm or less.
This is provided to prevent the substrate or soil for cultivation from leaking from the mesh holes of the mesh net 41, and if the size of the mesh holes is too large, the substrate or soil for cultivation will leak from the mesh holes, causing the loss of the substrate or soil for cultivation, which will increase the cultivation cost; if the size of the grid holes is too small, poor air permeability can be caused, the penetration of the seedling root system is influenced, and the culture period and effect can be increased. Meanwhile, the diameter of the grid line is less than or equal to 1mm, so that the area of the grid hole is increased to the maximum extent, the air permeability is increased, and the bearing capacity is also ensured.
In an exemplary embodiment, the screen 41 is a PVC mesh or a wire mesh with a corrosion resistant coating.
In an exemplary embodiment, the infusion tube 42 is a PVC tube or a steel tube with a corrosion resistant coating.
PVC (Polyvinyl chloride) is the most popular plastic in world production and has wide application, and the material can be manufactured by an integral forming method with low cost. The wire netting and the steel pipe with the anti-corrosion coating can obtain a better bearing effect.
In an exemplary embodiment, the height of the mycorrhizal fungi culture tank 2 is 10-15 cm.
The arrangement is to ensure that the arbuscular mycorrhizal fungi and the root system of the seedling have proper symbiotic space. The arbuscular mycorrhizal fungi substrate is expensive, and the height of the mycorrhizal fungi culture tank 2 is too high, so that the cost of using the arbuscular mycorrhizal fungi substrate is increased; the height of the mycorrhizal fungi culture tank 2 is too low and the symbiotic effect is reduced. Through actual verification, the mycorrhizal fungi culture tank 2 designed by the application can achieve the effect of three times that of a common growth culture box which is completely filled with the mycorrhizal fungi matrix, and the cost of the arbuscular mycorrhizal fungi matrix can be greatly reduced.
The application provides a culture apparatus of nursery stock arbuscular mycorrhizal fungi includes: a seedling planting groove 3, a mycorrhizal fungi culture groove 2 and a nutrient medium groove 1; the seedling planting tank 3, the mycorrhizal fungi culture tank 2 and the nutrient medium tank 1 are sequentially arranged in a stacked manner from top to bottom. The bottoms of the seedling planting tank 3 and the mycorrhizal fungi culture tank 2 are provided with air-permeable components 4, and the whole bottom is covered with the air-permeable components 4; the air-permeable assembly 4 comprises: a screen 41 and a perfusion tube 42, wherein the screen 41 is connected with the perfusion tube 42. The seedling planting groove 3 is used for planting seedling seedlings or seeds; the mycorrhizal fungi culture tank 2 is used for symbiotic culture of arbuscular mycorrhizal fungi and nursery stocks; the nutrient medium groove 1 is used for providing nutrient medium for the growth of the nursery stock and the arbuscular mycorrhizal fungi. The layer which is arranged independently is used for culturing the arbuscular mycorrhizal fungi, so that the arbuscular mycorrhizal fungi and the root system of the seedling have proper symbiotic space. By means of layered arrangement, the costs of the arbuscular mycorrhizal fungi matrix can be reduced, and the effect of promoting the growth of seedling root systems is achieved.
The detailed description provided above is only a few examples under the general concept of the present application, and does not constitute a limitation to the scope of the present application. Any other embodiments extended according to the scheme of the present application without inventive efforts will be within the scope of protection of the present application for a person skilled in the art.
Claims (8)
1. A culture apparatus for arbuscular mycorrhizal fungi of nursery stock, characterized by comprising: a seedling planting groove (3), a mycorrhizal fungi culture groove (2) and a nutrient medium groove (1);
the seedling planting tank (3), the mycorrhizal fungi culture tank (2) and the nutrient medium tank (1) are sequentially arranged in a stacked manner from top to bottom; openings are formed in the tops of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2), side walls are arranged on the peripheries of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2), air-permeable components (4) are arranged at the bottoms of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2), and the air-permeable components (4) are paved on the bottoms of the whole seedling planting groove (3) and the whole mycorrhizal fungi culture groove (2);
the air-permeable assembly (4) comprises: a screen (41) and a transfusion tube (42);
the screen (41) is connected with a liquid conveying pipe (42), and the screen (41) is connected with the side walls of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2);
the infusion tube (42) stretches across the bottoms of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2), two ends of the infusion tube are respectively connected with different side walls of the seedling planting groove (3) and the mycorrhizal fungi culture groove (2), and a plurality of holes are formed in the infusion tube (42).
2. The cultivation device of arbuscular mycorrhizal fungi of nursery stock according to claim 1, characterized in that, the cultivation device further comprises a liquid conveying tank (5), the liquid conveying tank (5) is arranged on the outer side wall of the nursery stock planting tank (3) and the mycorrhizal fungi cultivation tank (2), and the liquid conveying tank (5) is connected with the liquid conveying pipe (42).
3. The apparatus for culturing arbuscular mycorrhizal fungi of claim 1, wherein the number of the infusion tubes (42) is two, one of the infusion tubes (42) transversely passes through the bottom central axis position, and the other infusion tube (42) longitudinally passes through the bottom central axis position to form a cross structure.
4. The apparatus for culturing arbuscular mycorrhizal fungi of claim 1, wherein the number of the infusion tubes (42) is two, and the two infusion tubes (42) respectively pass through the diagonal positions at the bottom to form a cross structure.
5. The apparatus for culturing arbuscular mycorrhizal fungi of claim 1, wherein the mesh openings of the screen (41) have a side length of 2-3mm and a diameter of 1mm or less.
6. The apparatus for culturing arbuscular mycorrhizal fungi of claim 1, wherein the screen (41) is a PVC net or a wire net with a corrosion-resistant coating.
7. The apparatus for culturing arbuscular mycorrhizal fungi of claim 1, wherein the infusion tube (42) is a PVC tube or a steel tube with a corrosion-resistant coating.
8. The apparatus for culturing arbuscular mycorrhizal fungi of nursery stock according to claim 1, wherein the height of the mycorrhizal fungi culture tank (2) is 10-15 cm.
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| CN202220649612.3U CN217038070U (en) | 2022-03-23 | 2022-03-23 | Culture apparatus of nursery stock arbuscular mycorrhizal fungi |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500178A (en) * | 2022-10-25 | 2022-12-23 | 贵州大学 | Bird Wang Chajun seedling raising device and using method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115500178A (en) * | 2022-10-25 | 2022-12-23 | 贵州大学 | Bird Wang Chajun seedling raising device and using method thereof |
| CN115500178B (en) * | 2022-10-25 | 2024-01-09 | 贵州大学 | Bird king tea mycorrhiza seedling raising device and application method thereof |
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