CN216003813U - Cell culture medium storage and subpackage device - Google Patents
Cell culture medium storage and subpackage device Download PDFInfo
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- CN216003813U CN216003813U CN202120025026.7U CN202120025026U CN216003813U CN 216003813 U CN216003813 U CN 216003813U CN 202120025026 U CN202120025026 U CN 202120025026U CN 216003813 U CN216003813 U CN 216003813U
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Abstract
A cell culture medium storage and subpackage device comprises a culture medium storage tank and a fixed platform; a storage tank top cover is arranged at the top of the culture medium storage tank; the top cover of the storage tank is movably connected with the upper edge of the culture medium storage tank; the bottom of the culture medium storage tank is fixedly connected with the fixed platform; a liquid outlet is formed in the bottom of the culture medium storage tank; the liquid outlet is connected with a first transmission pipe; the first transmission pipe is movably connected with the liquid outlet; the first transmission pipe is provided with a micro-transmission pump; a culture medium inlet is arranged on the top cover of the storage tank; a second transmission pipe is connected and arranged on the culture medium inlet; the second transmission pipe is movably connected with the culture medium inlet; an interlayer is arranged on the outer wall of the culture medium storage tank; the interlayer is provided with a temperature regulating liquid inlet and a temperature regulating liquid outlet; the upper surface of the fixed table is provided with a groove; the split charging bottle is arranged in the groove. The utility model can accurately subpackage the culture medium and improve the subpackaging efficiency.
Description
Technical Field
The utility model relates to the technical field of cell culture equipment, in particular to a cell culture medium storage and subpackaging device.
Background
The cell culture refers to a method for simulating in vitro in vivo environment asepsis, proper temperature, pH value, certain nutritional conditions and the like to enable the cell to survive, grow and reproduce and maintain main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can be used for culturing a single cell into simple single cells or cells with few differentiation in a large scale, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.
In cell culture experiments, the preparation of a culture medium is one of the most basic operations and is also a very important step, particularly in the most comparative experiments, in order to control variables and reduce experimental errors, the components and the amount of the prepared culture medium need to be kept uniform, and when the culture medium is prepared manually, the problems of complicated operation and uneven split charging of the culture medium exist, and the accuracy of the experiments is influenced.
Disclosure of Invention
The utility model aims to avoid the defects of the prior art and provide a cell culture medium storage and subpackaging device, thereby effectively solving the problems of the prior art.
In order to achieve the purpose, the utility model adopts the technical scheme that:
a cell culture medium storage and split charging device comprises a culture medium storage tank 1 and a fixed platform 2; a storage tank top cover 3 is arranged at the top of the culture medium storage tank 1; the storage tank top cover 3 is movably connected with the upper edge of the culture medium storage tank 1; the bottom of the culture medium storage tank 1 is fixedly connected with the fixed platform 2;
a liquid outlet 4 is arranged at the bottom of the culture medium storage tank 1; a first transmission pipe 5 is connected to the liquid outlet 4; the first transmission pipe 5 is movably connected with the liquid outlet 4; a micro delivery pump 6 is arranged on the first delivery pipe 5;
a culture medium inlet 7 is arranged on the top cover 3 of the storage tank; a second transmission pipe 8 is connected and arranged on the culture medium inlet 7; the second transmission pipe 8 is movably connected with the culture medium inlet 7;
an interlayer 9 is arranged on the outer wall of the culture medium storage tank 1; a temperature regulating liquid inlet 10 and a temperature regulating liquid inlet 10 are arranged on the interlayer 9;
the upper surface of the fixed table 2 is provided with a groove 11; the split charging bottle is arranged in the groove 11.
Further, a cavity 12 is arranged inside the fixed table 2; a rotating motor 13 is arranged inside the cavity 12; a stirring blade 14 is arranged in the culture medium storage tank 1; the bottom of the stirring paddle 14 is provided with a stirring shaft 15; the tail end of the stirring shaft 15 penetrates through the bottom of the culture medium storage tank 1 and the rotating motor 13; the stirring paddle 14 is driven to rotate by the rotating motor 13.
Further, an additive liquid inlet 16 is arranged on the top cover 3 of the storage tank; a sealing plug 17 is movably arranged on the additive agent inlet 16.
Further, an acid-base test inlet 18 is formed in the top cover 3 of the storage tank; the acid-base test inlet 18 is provided with a sealing cover 19.
Further, a filtering cavity 20 is arranged at the joint of the culture medium inlet 7 and the second transmission pipe 8; a filtering membrane 21 is arranged in the filtering cavity 20; two ends of the filtering cavity 20 are respectively movably connected with the culture medium inlet 7 and the second transmission pipe 8.
Further, a liquid level meter 22 is arranged on the culture medium storage tank 1.
Further, a temperature sensor 23 is provided inside the medium storage tank 1.
Compared with the prior art, the utility model has at least the following beneficial effects:
1. the cell culture medium storage and subpackaging device can accurately subpackage the culture medium, so that the subpackaging efficiency is improved;
2. the liquid inlet is provided with the plurality of culture medium liquid inlets, so that the liquid components can be accurately mixed in real time, the components of the culture medium can be prepared in real time, and the convenience of operation is improved;
3. the culture medium storage box is provided with the temperature-regulating interlayer, so that the temperature of the culture medium in the device can be controlled, the culture medium is prevented from being solidified, blocked and wasted, and if the temperature of the culture medium is too high, the culture medium can be properly cooled;
4. the stirring device can keep the culture medium uniform, and avoid the situations of precipitation and uneven dispersion;
5. the filtering cavity 20 can filter the culture medium, so that the quality of the culture medium is ensured;
6. the disinfection, cleaning and drying can be finished by introducing disinfectant, clear water and wind into the culture medium inlet 7, so that the culture medium can be repeatedly used, is clean and sanitary, and avoids waste;
7. the utility model is provided with a liquid level meter, a temperature sensor and an acid-base test inlet 18, thereby facilitating the real-time monitoring of liquid level, temperature and acid-base conditions;
8. the conveying pipelines are movably connected, so that the replacement and the disinfection are convenient, and the pollution of a culture medium is avoided.
Drawings
FIG. 1 is a schematic front view of the present invention;
in the figure: a culture medium storage tank 1; a fixed table 2; a tank top cover 3; a liquid outlet 4; a first transfer pipe 5; a micro delivery pump 6; a culture medium inlet 7; a second transfer pipe 8; a barrier layer 9; a temperature adjusting liquid inlet 10; a groove 11; a cavity 12; a rotating motor 13; a stirring blade 14; a stirring shaft 15; an additive liquid inlet 16; a sealing plug 17; an acid-base test inlet 18; a seal cover 19; a filtering chamber 20; a filtration membrane 21; a liquid level gauge 22; a temperature sensor 23; a temperature regulating liquid outlet 24.
Detailed Description
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the utility model.
As shown in fig. 1:
example 1:
a cell culture medium storage and split charging device comprises a culture medium storage tank 1 and a fixed platform 2; a storage tank top cover 3 is arranged at the top of the culture medium storage tank 1; the storage tank top cover 3 is movably connected with the upper edge of the culture medium storage tank 1; the bottom of the culture medium storage tank 1 is fixedly connected with the fixed platform 2;
a liquid outlet 4 is arranged at the bottom of the culture medium storage tank 1; a first transmission pipe 5 is connected to the liquid outlet 4; the first transmission pipe 5 is movably connected with the liquid outlet 4; a micro delivery pump 6 is arranged on the first delivery pipe 5;
a culture medium inlet 7 is arranged on the top cover 3 of the storage tank; a second transmission pipe 8 is connected and arranged on the culture medium inlet 7; the second transmission pipe 8 is movably connected with the culture medium inlet 7;
an interlayer 9 is arranged on the outer wall of the culture medium storage tank 1; a temperature regulating liquid inlet 10 and a temperature regulating liquid outlet 24 are arranged on the interlayer 9;
the upper surface of the fixed table 2 is provided with a groove 11; the split charging bottle is arranged in the groove 11.
Example 2:
in addition to embodiment 1, a cavity 12 is provided inside the fixed table 2; a rotating motor 13 is arranged inside the cavity 12; a stirring blade 14 is arranged in the culture medium storage tank 1; the bottom of the stirring paddle 14 is provided with a stirring shaft 15; the tail end of the stirring shaft 15 penetrates through the bottom of the culture medium storage tank 1 and the rotating motor 13; the stirring paddle 14 is driven to rotate by the rotating motor 13.
Example 3:
on the basis of the embodiment 1-2, an additive liquid inlet 16 is arranged on the top cover 3 of the storage tank; a sealing plug 17 is movably arranged on the additive agent inlet 16.
Example 4:
on the basis of the embodiments 1-3, an acid-base test inlet 18 is arranged on the top cover 3 of the storage tank; the acid-base test inlet 18 is provided with a sealing cover 19.
Example 5:
on the basis of the embodiments 1-4, a filtering cavity 20 is arranged at the joint of the culture medium inlet 7 and the second transmission pipe 8; a filtering membrane 21 is arranged in the filtering cavity 20; two ends of the filtering cavity 20 are respectively movably connected with the culture medium inlet 7 and the second transmission pipe 8.
Example 6:
in addition to examples 1 to 5, a level gauge 22 was provided on the medium storage tank 1.
Example 7:
in addition to examples 1 to 6, a temperature sensor 23 is provided inside the medium storage tank 1.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (7)
1. A cell culture medium storage and subpackage device is characterized by comprising a culture medium storage tank (1) and a fixed platform (2); a storage tank top cover (3) is arranged at the top of the culture medium storage tank (1); the storage tank top cover (3) is movably connected with the upper edge of the culture medium storage tank (1); the bottom of the culture medium storage tank (1) is fixedly connected with the fixed platform (2);
a liquid outlet (4) is arranged at the bottom of the culture medium storage tank (1); a first transmission pipe (5) is connected to the liquid outlet (4); the first transmission pipe (5) is movably connected with the liquid outlet (4); a micro delivery pump (6) is arranged on the first delivery pipe (5);
a culture medium inlet (7) is arranged on the top cover (3) of the storage tank; a second transmission pipe (8) is connected and arranged on the culture medium inlet (7); the second transmission pipe (8) is movably connected with the culture medium inlet (7);
an interlayer (9) is arranged on the outer wall of the culture medium storage tank (1); a temperature regulating liquid inlet (10) and a temperature regulating liquid outlet (24) are arranged on the interlayer (9);
the upper surface of the fixed table (2) is provided with a groove (11); the split charging bottle is arranged in the groove (11).
2. A cell culture medium storage and dispensing apparatus as claimed in claim 1, wherein the fixed platform (2) is provided with a cavity (12) therein; a rotating motor (13) is arranged in the cavity (12); a stirring paddle (14) is arranged in the culture medium storage tank (1); a stirring shaft (15) is arranged at the bottom of the stirring blade (14); the tail end of the stirring shaft (15) penetrates through the bottom of the culture medium storage tank (1) and the rotating motor (13); the rotating motor (13) drives the stirring paddle (14) to rotate.
3. The cell culture medium storage and dispensing device according to claim 2, wherein the storage tank top cover (3) is provided with an additive liquid inlet (16); a sealing plug (17) is movably arranged on the additive liquid inlet (16).
4. The cell culture medium storage and dispensing device according to claim 1, wherein the storage tank top cover (3) is provided with an acid-base test inlet (18); a sealing cover (19) is arranged on the acid-base test inlet (18).
5. A cell culture medium storage and dispensing device according to claim 1, wherein a filtering cavity (20) is arranged at the joint of the culture medium inlet (7) and the second conveying pipe (8); a filtering membrane (21) is arranged in the filtering cavity (20); two ends of the filtering cavity (20) are respectively and movably connected with the culture medium inlet (7) and the second transmission pipe (8).
6. A cell culture medium storage and dispensing apparatus according to claim 1, wherein the medium storage tank (1) is provided with a liquid level meter (22).
7. A cell culture medium storage and dispensing apparatus according to claim 1, wherein the medium storage tank (1) is provided with a temperature sensor (23) therein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120025026.7U CN216003813U (en) | 2021-01-06 | 2021-01-06 | Cell culture medium storage and subpackage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120025026.7U CN216003813U (en) | 2021-01-06 | 2021-01-06 | Cell culture medium storage and subpackage device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216003813U true CN216003813U (en) | 2022-03-11 |
Family
ID=80581259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120025026.7U Active CN216003813U (en) | 2021-01-06 | 2021-01-06 | Cell culture medium storage and subpackage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216003813U (en) |
-
2021
- 2021-01-06 CN CN202120025026.7U patent/CN216003813U/en active Active
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