CN215931921U - Full-automatic application of sample device - Google Patents

Abstract

The utility model discloses a full-automatic sample adding device, which comprises: the device comprises a reagent needle mechanism, a sample adding needle mechanism, a supply device for providing an extraction strip, a PCR tube and a reagent, a gantry mechanism and a control device; the reagent needle mechanism and the sample adding needle mechanism are both connected with the portal frame mechanism, and the portal frame mechanism can transversely move along the feeding device; the reagent needle mechanism, the sample adding needle mechanism and the portal frame mechanism are all connected with the control device. The device can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reduce the labor intensity of operators and be beneficial to improving the operation efficiency and the operation accuracy.

Description

Full-automatic application of sample device

Technical Field

The utility model relates to the technical field of molecular diagnosis, in particular to a full-automatic sample adding device.

Background

In molecular diagnosis, nucleic acid needs to be extracted and then quantitatively analyzed by an instrument, and in an automatic extraction amplification molecular diagnosis device, the instrument needs to stably extract nucleic acid. In the prior art, generally, after a TIP head is pricked by a pipette, extraction and transfer operations of a reagent and a sample liquid are performed, but the manual operation intensity is high due to the mode, and automatic detection operation of nucleic acid cannot be realized.

In summary, how to automatically extract various reagents and sample solutions required for nucleic acid detection is an urgent problem to be solved by those skilled in the art.

SUMMERY OF THE UTILITY MODEL

In view of this, the present invention provides a full-automatic sample adding device, which can automatically complete the operations of extracting and adding sample liquid and reagent, effectively reduce the labor intensity of operators, and is beneficial to improving the operation efficiency and the operation accuracy.

In order to achieve the above purpose, the utility model provides the following technical scheme:

a full-automatic sample adding device comprises: the device comprises a reagent needle mechanism, a sample adding needle mechanism, a supply device, a gantry mechanism and a control device, wherein the reagent needle mechanism is used for extracting a corresponding reagent and adding the reagent to an extraction strip or a PCR tube;

the reagent needle mechanism and the sample adding needle mechanism are both connected with the portal frame mechanism, and the portal frame mechanism can transversely move along the feeding device;

the reagent needle mechanism, the sample adding needle mechanism and the gantry mechanism are all connected with the control device.

Preferably, the portal frame mechanism comprises a portal frame and a traversing device for driving the portal frame to transversely move left and right, and the reagent needle mechanism and the sample injection needle mechanism are respectively arranged on two sides of the portal frame;

the extraction strip, the PCR tube and the reagent bin for placing the reagent are all arranged at the top of the supply device, the supply device is provided with a guide device for limiting the moving track of the portal frame, the portal frame is connected with the guide device, and the traversing device is connected with the control device.

Preferably, the guide device comprises a linear slide rail and a slide block arranged on the linear slide rail in a sliding manner, the portal frame is arranged on the slide block, and the slide block is connected with the traversing device;

or the guide device comprises a guide sleeve and a guide post embedded in the guide sleeve, the guide post is connected with the transverse moving device, and the portal frame is arranged on the guide post.

Preferably, the reagent needle mechanism comprises a reagent needle, a longitudinal movement device for driving the reagent needle to move back and forth, and a reagent lifting device for driving the reagent needle to move up and down, the longitudinal movement device and the reagent lifting device are both connected with the reagent needle, and the longitudinal movement device and the reagent lifting device are both connected with the control device.

Preferably, the top of the supply device is provided with a TIP and a PCR cap, and the reagent needle can prick the TIP and the PCR cap.

Preferably, the position of the sample adding needle mechanism corresponds to the position of the extraction strip, and when the sample adding needle mechanism is lifted or lowered, the extraction strip can be subjected to liquid extraction or liquid injection operation.

Preferably, the sample adding needle mechanism comprises a sample adding needle, a sample adding pump for driving the sample adding needle to pump the liquid, a first lifting device for driving a plunger of the sample adding pump to move up and down, and a second lifting device for driving the sample adding needle to move up and down, and the sample adding pump is communicated with the sample adding needle;

the first lifting device is connected with the plunger, the second lifting device is connected with the sampling needle, and the first lifting device and the second lifting device are both connected with the control device.

Preferably, the sample adding needle mechanism comprises a plurality of sample adding needles and sample adding pumps, the sample adding pumps are communicated in a one-to-one correspondence mode, the number of the sample adding needles is the same as the number of the extraction strips, and the positions of the sample adding needles correspond to the positions of the extraction strips one by one.

Preferably, the anti-dripping mechanism is arranged on the portal frame mechanism and comprises a liquid pocket box for collecting liquid drops and a driving piece for driving the liquid pocket box to move left and right transversely;

when reagent needle mechanism is in the state that rises, the driving piece can drive pocket liquid box removes to the below of reagent needle mechanism, works as when application of sample needle mechanism is in the state that rises, the driving piece can drive pocket liquid box removes to application of sample needle mechanism's below.

Preferably, still include the waste liquid treatment mechanism that is used for collecting the waste liquid, application of sample needle mechanism can extract draw the waste liquid in the strip, and remove the waste liquid to waste liquid treatment mechanism.

Preferably, the waste liquid treatment mechanism includes a waste liquid box for collecting waste liquid, a float switch for detecting whether the capacity of the waste liquid box reaches a preset capacity, and a liquid suction pipe for evacuating waste liquid in the waste liquid box, and the float switch and the liquid suction pipe are connected to the control device.

When the full-automatic sample adding device provided by the utility model is used, firstly, the portal frame mechanism can be driven to transversely move along the supply device, so that the reagent needle mechanism moves to a position which is parallel to the reagent bin, then the reagent needle mechanism is controlled to extract a corresponding reagent, then the reagent needle mechanism is controlled to leave the reagent bin, then the portal frame mechanism is controlled to transversely move, so that the reagent needle mechanism moves to a position which is parallel to the extraction strip, then the reagent needle mechanism is controlled to add the reagent into the extraction strip, and the operation is repeated until all required reagents are injected into the extraction strip;

then, controlling the gantry mechanism to move transversely, so that the sample adding needle mechanism moves to a position aligned with the extraction strip, then controlling the sample adding needle mechanism to perform sample liquid pumping operation on the extraction strip, driving the sample adding needle mechanism to perform repeated lifting operation, and performing repeated liquid pumping operation on the extraction strip so as to fully and uniformly mix the sample liquid and the reagent; later control application of sample needle mechanism leaves and draws the strip, then, control portal frame mechanism lateral shifting, make reagent needle mechanism move to the position of drawing the strip parallel and level, the sample liquid after the control reagent needle mechanism absorbs the mixing again, the control reagent needle mechanism leaves and draws the strip again, later, control portal frame mechanism lateral shifting, make reagent needle mechanism move to the position with PCR pipe parallel and level, it is intraductal to control reagent needle mechanism again and pour into the PCR with the sample liquid, finally, the extraction and the interpolation operation of sample liquid and reagent have been accomplished, this process need not artifical the participation, can effectively improve operating efficiency and accuracy.

In conclusion, the full-automatic sample adding device provided by the utility model can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reduce the labor intensity of operators, and is beneficial to improving the operation efficiency and the operation accuracy.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a fully automatic sample adding device provided by the present invention;

FIG. 2 is a schematic structural view of the full-automatic sample adding device from another view angle;

FIG. 3 is a schematic structural view of the fully automatic sample adding device from another view angle;

FIG. 4 is a schematic view of the structure of the drip-proof mechanism moving to the lower part of the reagent needle mechanism;

FIG. 5 is a schematic view of the structure of the drip-proof mechanism moving to the lower part of the sample injection needle mechanism;

FIG. 6 is a schematic view of the structure of the waste liquid treatment mechanism.

In fig. 1-6:

1 is a reagent needle mechanism, 11 is a reagent needle, 12 is a longitudinal moving device, 121 is a second linear slide rail, 122 is a second synchronous belt transmission structure, 123 is a longitudinal moving motor, 13 is a reagent lifting device, 2 is a sample adding needle mechanism, 21 is a sample adding needle, 22 is a sample adding pump, 23 is a first lifting device, 24 is a second lifting device, 25 is a moving plate, 3 is a supply device, 31 is a first linear slide rail, 32 is a TIP, 4 is a portal frame mechanism, 41 is a portal frame, 42 is a traversing device, 421 is a traversing motor, 422 is a first synchronous belt transmission structure, 5 is an extraction strip, 6 is a PCR tube, 7 is a reagent bin, 71 is a reagent hole, 8 is a drip-proof mechanism, 81 is a liquid pocket box, 82 is a first connecting rod, 83 is a second connecting rod, 84 is a roller, 85 is a fan, 86 is an exhaust pipe, 9 is a waste liquid treatment mechanism, 91 is a waste liquid box, 92 is a float switch, and 93 is a liquid extraction pipe.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The core of the utility model is to provide a full-automatic sample adding device, which can automatically complete the extraction and addition operation of sample liquid and reagent, effectively reduce the labor intensity of operators and is beneficial to improving the operation efficiency and the operation accuracy.

Referring to fig. 1 to fig. 6, fig. 1 is a schematic structural diagram of a full-automatic sample adding device provided by the present invention; FIG. 2 is a schematic structural view of the full-automatic sample adding device from another view angle; FIG. 3 is a schematic structural view of the fully automatic sample adding device from another view angle; FIG. 4 is a schematic view of the structure of the drip-proof mechanism moving to the lower part of the reagent needle mechanism; FIG. 5 is a schematic view of the structure of the drip-proof mechanism moving to the lower part of the sample injection needle mechanism; FIG. 6 is a schematic view of the structure of the waste liquid treatment mechanism.

This embodiment provides a full-automatic application of sample device, includes: a reagent needle mechanism 1 for extracting a corresponding reagent and adding the reagent to the extraction strip 5 or the PCR tube 6, a sample injection needle mechanism 2 for performing a sample liquid extraction and injection operation on the extraction strip 5, a supply device 3 for supplying the extraction strip 5, the PCR tube 6 and the reagent, a portal frame mechanism 4 and a control device; the reagent needle mechanism 1 and the sample adding needle mechanism 2 are both connected with a portal frame mechanism 4, and the portal frame mechanism 4 can transversely move along the supply device 3; the reagent needle mechanism 1, the sample adding needle mechanism 2 and the gantry mechanism 4 are all connected with the control device.

It should be noted that the reagent needle mechanism 1 is responsible for completing the extraction and addition operations of the reagent, the sample adding needle mechanism 2 is responsible for completing the addition and mixing operations of the sample, and the portal frame mechanism 4 is responsible for the transfer and positioning of the reagent needle mechanism 1 and the sample adding needle mechanism 2 so as to realize the synchronous corresponding operation of multiple channels and further greatly improve the operation efficiency of the device.

In the actual application process, the shapes, structures, sizes, materials, positions and the like of the reagent needle mechanism 1, the sample adding needle mechanism 2, the supply device 3, the gantry mechanism 4 and the control device can be determined according to actual conditions and actual requirements.

When the full-automatic sample adding device provided by the utility model is used, firstly, the gantry mechanism 4 can be driven to transversely move along the supply device 3, so that the reagent needle mechanism 1 moves to a position which is parallel to the reagent bin 7, then the reagent needle mechanism 1 is controlled to extract a corresponding reagent, then the reagent needle mechanism 1 is controlled to leave the reagent bin 7, then the gantry mechanism 4 is controlled to transversely move, so that the reagent needle mechanism 1 moves to a position which is parallel to the extraction strip 5, then the reagent needle mechanism 1 is controlled to add the reagent into the extraction strip 5, and the operation is repeated until all required reagents are injected into the extraction strip 5;

then, controlling the portal frame mechanism 4 to move transversely, so that the sampling needle mechanism 2 moves to a position aligned with the extraction strip 5, then controlling the sampling needle mechanism 2 to perform sample liquid pumping and injecting operation on the extraction strip 5, and driving the sampling needle mechanism 2 to perform repeated lifting operation so as to perform repeated liquid pumping and injecting operation on the extraction strip 5, so that the sample liquid and the reagent are fully and uniformly mixed; later control application of sample needle mechanism 2 and leave and draw strip 5, then, control 4 lateral shifting of portal frame mechanism, make reagent needle mechanism 1 move to the position of drawing 5 parallel and level, control reagent needle mechanism 1 again and absorb the sample liquid after the mixing, control reagent needle mechanism 1 again and leave and draw strip 5, later, control 4 lateral shifting of portal frame mechanism, make reagent needle mechanism 1 move to the position with 6 parallel and level of PCR pipe, control reagent needle mechanism 1 again and pour into PCR pipe 6 with the sample liquid, finally accomplished the extraction and the interpolation operation of sample liquid and reagent, this process need not artifical the participation, can effectively improve operating efficiency and accuracy.

In conclusion, the full-automatic sample adding device provided by the utility model can automatically complete the extraction and addition operations of the sample liquid and the reagent, effectively reduce the labor intensity of operators, and is beneficial to improving the operation efficiency and the operation accuracy.

On the basis of the above embodiment, preferably, the gantry mechanism 4 includes a gantry 41 and a traversing device 42 for driving the gantry 41 to move laterally left and right, and the reagent needle mechanism 1 and the sample injection needle mechanism 2 are respectively disposed on two sides of the gantry 41; the extraction strip 5, the PCR tube 6 and the reagent bin 7 for placing the reagent are arranged at the top of the supply device 3, the supply device 3 is provided with a guide device for limiting the moving track of the portal frame 41, the portal frame 41 is connected with the guide device, and the traversing device 42 is connected with the control device.

Preferably, the guiding device comprises a linear slide rail and a slide block arranged on the linear slide rail in a sliding manner, the portal frame 41 is arranged on the slide block, and the slide block is connected with the traversing device 42; or the guiding device comprises a guide sleeve and a guide post embedded in the guide sleeve, the guide post is connected with the transverse moving device 42, and the portal frame 41 is arranged on the guide post.

It should be noted that, because the reagent needle mechanism 1 and the sample adding needle mechanism 2 are respectively disposed on the left and right sides of the portal frame 41, when the control device drives the traverse device 42 to move transversely, the traverse device 42 can drive the portal frame 41 to move transversely, and further drive the reagent needle mechanism 1 and the sample adding needle mechanism 2 to move transversely, so that the reagent needle mechanism 1 and the sample adding needle mechanism 2 reach the required positions to perform corresponding operations.

For example, a first linear slide rail 31 may be disposed on the supply device 3, the traverse device 42 includes a traverse motor 421 and a first synchronous belt transmission structure 422, the first synchronous belt transmission structures 422 are disposed on two sides of the first linear slide rail 31, the traverse motor 421 can drive a synchronous belt of the first synchronous belt transmission structure 422 to rotate when rotating, a first slider capable of sliding along the first linear slide rail 31 is disposed on the synchronous belt, and the portal frame 41 is fixedly connected to the first sliders on two sides, so that when the control device drives the traverse motor 421 to operate, the synchronous belt driving the first synchronous belt transmission structure 422 rotates, so that the first slider drives the portal frame 41 to slide along the first linear slide rail 31, thereby ensuring that the reagent needle mechanism 1 and the sample application needle mechanism 2 can reach a desired position to perform corresponding operations.

It should be noted that, in addition to the traverse motor 421 driving the synchronous belt to rotate, a pneumatic or other electric device may also be used to drive, and in addition to the synchronous belt driving manner, a rack and pinion or other transmission mechanism may also be used to implement the transverse movement operation of the gantry 41. Besides, the linear slide rail can be used for guiding, and the guide column and the guide sleeve or other guide mechanisms can be used for guiding. The shape, structure, size, position, etc. of the gantry 41, the traverse device 42, and the guide device can be determined according to actual conditions and actual requirements during actual use.

Preferably, the reagent needle mechanism 1 comprises a reagent needle 11, a longitudinal moving device 12 for driving the reagent needle 11 to move back and forth, and a reagent lifting device 13 for driving the reagent needle 11 to move up and down, wherein the longitudinal moving device 12 and the reagent lifting device 13 are both connected with the reagent needle 11, and the longitudinal moving device 12 and the reagent lifting device 13 are both connected with the control device.

It should be noted that the longitudinal moving device 12 may include a second linear slide rail 121 disposed along the Y-axis direction, a second synchronous belt transmission structure 122 disposed parallel to the second linear slide rail 121, and a longitudinal moving motor 123, where the longitudinal moving motor 123 is connected to the control device, a second slider capable of sliding along the second linear slide rail 121 is disposed on a synchronous belt of the second synchronous belt transmission structure 122, and the reagent needle 11 is fixedly connected to the second slider, so when the control device drives the longitudinal moving motor 123 to operate, a synchronous belt of the second synchronous belt transmission structure 122 may be driven to rotate, and when the synchronous belt rotates, the second slider may drive the reagent needle 11 to slide along the second linear slide rail 121, thereby ensuring that the reagent needle 11 can reach a desired position to perform corresponding operation.

It should be further noted that the reagent lifting device 13 may include a reagent lifting motor, and the reagent lifting motor is connected to the control device, and when the control device drives the reagent lifting motor to operate, the adaptor of the reagent needle 11 may be driven to prick the TIP32, thereby implementing the operations of extracting and adding the reagent.

In the actual operation process, the shape, structure, size, position, etc. of the reagent needle 11, the longitudinal moving device 12, and the reagent lifting device 13 can be determined according to the actual situation and the actual requirement.

Preferably, the top of the supply device 3 is provided with a TIP32 and a PCR cap, and the TIP32 and the PCR cap can be pricked by the reagent needle 11. Wherein TIP32 is used to cooperate with the reagent needle 11 to suck up liquid, and the PCR cap is used to cover and seal the PCR tube 6.

For example, the gantry 41 may be driven to move laterally by the traverse motor 421 so that the reagent needle mechanism 1 is positioned flush with the TIP32, and then the traverse motor 123 may be controlled to drive the reagent needle mechanism 1 to move forward and backward so that the reagent needle 11 is aligned with the TIP32, and then the reagent needle 11 may be controlled to prick the TIP32 by the reagent lifting motor, and when the reagent needle 11 is successfully pricked into the TIP32, the reagent needle 11 may be controlled to lift. Then, the gantry 41 is driven by the traversing motor 421 to move transversely, and the reagent needle mechanism 1 is driven to move back and forth by controlling the longitudinal moving motor 123, so that the reagent needle mechanism 1 moves right above the reagent bin 7, and the top of the reagent bin 7 is provided with a reagent hole 71, so that the reagent needle 11 can suck a reagent. Then, the reagent lift motor controls the reagent needle 11 to cooperate with the TIP32 to suck the reagent, and when the reagent needle 11 successfully extracts the reagent, the reagent lift motor controls the reagent needle 11 to lift. Then, the gantry 41 is driven to move transversely by the traversing motor 421, the reagent needle mechanism 1 is driven to move back and forth by controlling the longitudinal moving motor 123, so that the reagent needle mechanism 1 moves right above the extraction strip 5, the reagent needle 11 is controlled by the reagent lifting motor to inject the reagent into the corresponding reaction bin, and the process is repeated until all the required reaction bins are filled with the corresponding reagent. Similarly, the reagent needle mechanism 1 can be driven to extract the uniformly mixed sample liquid through similar operation, and the uniformly mixed sample liquid is injected into the corresponding PCR tube 6, and the reagent needle mechanism 1 can also cover the PCR tube 6 by using a PCR cover in a sealing manner, so that the sealed PCR tube 6 filled with the sample liquid can be detected subsequently.

In the actual operation process, the shape, structure, size, material, position, etc. of the TIP32, the PCR lid, the PCR tube 6, and the reagent cartridge 7 on the supply device 3 can be determined according to actual conditions and actual requirements.

In addition to the above embodiments, it is preferable that the position of the sample addition needle mechanism 2 is set to correspond to the position of the extraction strip 5, and when the sample addition needle mechanism 2 is operated to ascend or descend, the extraction strip 5 can be subjected to a liquid extraction or injection operation.

Preferably, the sample adding needle mechanism 2 comprises a sample adding needle 21, a sample adding pump 22 for driving the sample adding needle 21 to pump the liquid, a first lifting device 23 for driving a plunger of the sample adding pump 22 to move up and down, and a second lifting device 24 for driving the sample adding needle 21 to move up and down, wherein the sample adding pump 22 is communicated with the sample adding needle 21; the first lifting device 23 is connected with the plunger, the second lifting device 24 is connected with the sampling needle 21, and both the first lifting device 23 and the second lifting device 24 are connected with the control device. Therefore, the control device controls the first lifting device 23 and the second lifting device 24 to synchronously descend, so that the injection operation of the sample liquid can be realized, and the control device controls the first lifting device 23 and the second lifting device 24 to synchronously ascend, so that the pumping operation of the sample liquid can be realized. The repeated liquid pumping and injecting process of the sample adding needle 21 can effectively mix the sample liquid and various reagents uniformly.

Preferably, the sample adding needle mechanism 2 comprises a plurality of sample adding needles 21 and sample adding pumps 22, the sample adding pumps 22 are communicated with the sample adding needles 21 in a one-to-one correspondence manner, the number of the sample adding needles 21 is the same as that of the extraction strips 5, and the positions of the sample adding needles 21 are in one-to-one correspondence with the positions of the extraction strips 5. For example, can set up 8 application of sample needles 21 side by side, 8 application of sample needles 21 and 8 draw 5 one-to-one of strip to make the device structure more compact, it is little to occupy instrument space, and, can realize multichannel synchronous operation, in order effectively to shorten the application of sample time, improve operating efficiency.

For example, the traversing motor 421 can be used to drive the gantry 41 to move laterally, so as to position the sampling needle mechanism 2 above the extraction strip 5, the first lifting device 23 and the second lifting device 24 are used to drive the sampling needle mechanism 2 to extract the sample liquid in the extraction strip 5, and the traversing motor 421 is used to drive the gantry 41 to move laterally, so as to move the sampling needle mechanism 2 to a position above the reaction bin of the extraction strip 5, so as to inject the sample liquid into the corresponding reaction bin, and the above operations are repeated until all the required reaction bins are injected with the corresponding sample liquid and mixed uniformly.

In the actual application process, the shapes, structures, sizes, positions, and the like of the sample adding needle 21, the sample adding pump 22, the first elevating device 23, and the second elevating device 24 can be determined according to actual conditions and actual requirements.

On the basis of the above embodiment, preferably, the drip-proof device further comprises a drip-proof mechanism 8 arranged on the gantry mechanism 4, wherein the drip-proof mechanism 8 comprises a liquid pocket box 81 for collecting liquid drops and a driving member for driving the liquid pocket box 81 to move left and right laterally; when the reagent needle mechanism 1 is in the ascending state, the driving member can drive the pocket liquid box 81 to move to the lower part of the reagent needle mechanism 1, and when the sample adding needle mechanism 2 is in the ascending state, the driving member can drive the pocket liquid box 81 to move to the lower part of the sample adding needle mechanism 2.

It should be noted that the sample adding needle mechanism 2 and the reagent needle mechanism 1 work alternately, and after moving up, a certain mechanism needs to drive corresponding liquid to move spatially, so that a liquid pocket box 81 needs to be arranged below the mechanism to effectively reduce liquid dripping and avoid the pollution phenomenon of the equipment. Each sample adding needle 21 can be fixed on the same moving plate 25, and when the sample adding needle 21 is lifted, the moving plate 25 is lifted synchronously.

Preferably, the driving member may include: the device comprises a sliding plate, a fixed block fixedly arranged on the portal frame 41, a first connecting rod 82 hinged with the fixed block, a second connecting rod 83 hinged with the first connecting rod 82 and a sliding block hinged with the second connecting rod 83, wherein the sliding block is arranged on the sliding plate, the first connecting rod 82 and the second connecting rod 83 are hinged and fixed through a rotating shaft of a roller 84, a liquid pocket box 81 is fixedly connected with the sliding plate, the liquid pocket box 81 is arranged below the sliding plate in parallel, and the fixed plate is provided with a sliding rail which is in sliding fit with the sliding plate, as shown in fig. 4 and 5.

When the moving plate 25 moves downwards to a position contacting with the driving part, the moving plate 25 continues to move downwards along with the moving plate 25, the moving plate 25 applies a leftward acting force on the roller 84, when the roller 84 moves leftwards due to a horizontal leftward external force, the first connecting rod 82 and the second connecting rod 83 push the sliding block leftwards through the action of the rotating shaft, the sliding block can drive the sliding plate to move leftwards together, and the liquid pocket box 81 is fixed below the sliding plate, so that the sliding plate can drive the liquid pocket box 81 to move leftwards synchronously, and at the moment, the liquid pocket groove on the left side of the liquid pocket box 81 is positioned right below the reagent needle mechanism 1 on the left side of the portal frame 41, so as to prevent the reagent needle mechanism 1 from dripping during the moving process.

When the moving plate 25 moves upwards, the moving plate 25 is separated from the driving member, that is, after the external force on the roller 84 is removed, the elastic member can move the sliding plate to the right to drive the liquid pocket box 81 to move to the right, and at this time, the liquid pocket groove on the right side of the liquid pocket box 81 is positioned right below the sampling needle mechanism 2 on the right side of the portal frame 41, so as to prevent the reagent from dropping from the sampling needle mechanism 2 during the moving process. This device utilizes elastic component and link mechanism to realize the operation of stretching out and evading of pocket liquid box 81 to guarantee that the operation of stretching out and retracting of pocket liquid box 81 goes on smoothly, make the left and right sides position switch of pocket liquid box 81 stable. In addition, a fan 85 and an exhaust pipe 86 can be further arranged to discharge reagent aerial fog on two sides of the liquid pocket box 81 outwards, so that the pollution phenomenon of the aerial fog to equipment is avoided.

The shape, structure, size, position, etc. of the pocket box 81, the driving member, and the moving plate 25 may be determined according to actual conditions and actual requirements during actual use.

Preferably, the device further comprises a waste liquid treatment mechanism 9 for collecting waste liquid, and the sample adding needle mechanism 2 can extract the waste liquid in the extraction strip 5 and move the waste liquid to the waste liquid treatment mechanism 9.

Preferably, the waste liquid disposal mechanism 9 includes a waste liquid box 91 for collecting waste liquid, a float switch 92 for detecting whether the capacity of the waste liquid box 91 reaches a preset capacity, and an extraction tube 93 for evacuating waste liquid in the waste liquid box 91, and both the float switch 92 and the extraction tube 93 are connected to the control device.

It should be noted that, after the sample liquid in the extraction strip 5 is transferred to the PCR tube 6 for detection, a part of liquid in the extraction strip 5 remains, at this time, the remaining waste liquid can be absorbed by the sampling needle mechanism 2, and the waste liquid is transferred to the waste liquid treatment mechanism 9, when the amount of the waste liquid in the waste liquid box 91 reaches a set value, the float switch 92 is triggered, the float switch 92 feeds back the signal to the control device, and the control device can start the liquid pumping tube 93 to pump out the waste liquid, so as to complete the cleaning work of the remaining waste liquid, thereby effectively improving the stability and safety of the device.

The shape, structure, size, position, etc. of the waste liquid box 91, the float switch 92, and the liquid suction pipe 93 can be determined in actual use according to actual conditions and actual requirements.

It should be noted that the full-automatic sample adding device provided by the utility model can realize automatic sample adding operation and effectively prevent pollution caused by manual intervention. Moreover, the device is provided with the corresponding drip-proof mechanism 8 and the waste liquid treatment mechanism 9, so that the using effect, the structural stability and the safety of the device are effectively improved, and the device can be popularized and used.

It should be noted that, in this document, the first lifting device 23 and the second lifting device 24, the first synchronous belt transmission structure 422 and the second synchronous belt transmission structure 122, the first linear guideway 31 and the second linear guideway 121, and the first slider and the second slider are mentioned, where the first and the second are only for distinguishing the difference of the positions, and are not sequentially distinguished.

It should be noted that the directions and positional relationships indicated by "up and down", "front and back", "left and right" in the present application are based on the directions and positional relationships shown in the drawings, and are only for the convenience of simplifying the description and facilitating the understanding, and do not indicate or imply that the device or element referred to must have a specific direction, be configured and operated in a specific direction, and thus, should not be construed as limiting the present invention.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. Any combination of all the embodiments provided by the present invention is within the scope of the present invention, and will not be described herein.

The fully automatic sample adding device provided by the utility model is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.

Claims (11)

1. The utility model provides a full-automatic application of sample device which characterized in that includes: a reagent needle mechanism (1) for extracting corresponding reagents and adding the reagents to an extraction strip (5) or a PCR tube (6), a sample adding needle mechanism (2) for performing sample liquid extraction and injection operation on the extraction strip (5), a supply device (3) for providing the extraction strip (5), the PCR tube (6) and the reagents, a gantry mechanism (4) and a control device;

the reagent needle mechanism (1) and the sample adding needle mechanism (2) are both connected with the gantry mechanism (4), and the gantry mechanism (4) can move transversely along the feeding device (3);

the reagent needle mechanism (1), the sample adding needle mechanism (2) and the gantry mechanism (4) are all connected with the control device.

2. The full-automatic sample adding device according to claim 1, wherein the gantry mechanism (4) comprises a gantry (41) and a traversing device (42) for driving the gantry (41) to move transversely, and the reagent needle mechanism (1) and the sample adding needle mechanism (2) are respectively arranged on two sides of the gantry (41);

the extraction strip (5), the PCR tube (6) and a reagent bin (7) for placing reagents are all arranged at the top of the supply device (3), the supply device (3) is provided with a guide device for limiting the moving track of the portal frame (41), the portal frame (41) is connected with the guide device, and the traversing device (42) is connected with the control device.

3. The full-automatic sample adding device according to claim 2, wherein the guiding device comprises a linear slide rail and a slide block slidably arranged on the linear slide rail, the gantry (41) is arranged on the slide block, and the slide block is connected with the traverse device (42);

or the guide device comprises a guide sleeve and a guide post embedded in the guide sleeve, the guide post is connected with the transverse moving device (42), and the portal frame (41) is arranged on the guide post.

4. The full-automatic sample adding device according to claim 1, wherein the reagent needle mechanism (1) comprises a reagent needle (11), a longitudinal moving device (12) for driving the reagent needle (11) to move back and forth, and a reagent lifting device (13) for driving the reagent needle (11) to move up and down, the longitudinal moving device (12) and the reagent lifting device (13) are both connected with the reagent needle (11), and the longitudinal moving device (12) and the reagent lifting device (13) are both connected with the control device.

5. The fully automatic sample adding device according to claim 4, wherein the top of the supplying device (3) is provided with a TIP (32) and a PCR cover, and the reagent needle (11) can prick the TIP (32) and the PCR cover.

6. The fully automatic sample adding device according to any one of claims 1 to 5, wherein the position of the sample adding needle mechanism (2) is arranged corresponding to the position of the extraction strip (5), and when the sample adding needle mechanism (2) is lifted or lowered, the extraction strip (5) can be subjected to liquid extraction or injection operation.

7. The fully automatic sample adding device according to claim 6, wherein the sample adding needle mechanism (2) comprises a sample adding needle (21), a sample adding pump (22) for driving the sample adding needle (21) to pump liquid, a first lifting device (23) for driving a plunger of the sample adding pump (22) to move up and down, and a second lifting device (24) for driving the sample adding needle (21) to move up and down, wherein the sample adding pump (22) is communicated with the sample adding needle (21);

the first lifting device (23) is connected with the plunger, the second lifting device (24) is connected with the sample adding needle (21), and the first lifting device (23) and the second lifting device (24) are both connected with the control device.

8. The full-automatic sample adding device according to claim 7, wherein the sample adding needle mechanism (2) comprises a plurality of sample adding needles (21) and sample adding pumps (22), the sample adding pumps (22) and the sample adding needles (21) are communicated in a one-to-one correspondence manner, the number of the sample adding needles (21) is the same as that of the extraction strips (5), and the positions of the sample adding needles (21) are in a one-to-one correspondence manner with the positions of the extraction strips (5).

9. The full-automatic sample adding device according to any one of claims 1 to 5, further comprising a drip prevention mechanism (8) arranged on the gantry mechanism (4), wherein the drip prevention mechanism (8) comprises a liquid pocket box (81) for collecting liquid drops and a driving member for driving the liquid pocket box (81) to move transversely;

the driving member includes: the liquid storage device comprises a sliding plate, a fixed block fixedly arranged on a gantry mechanism (4), a first connecting rod (82) hinged to the fixed block, a second connecting rod (83) hinged to the first connecting rod (82) and a sliding block hinged to the second connecting rod (83), wherein the sliding block is arranged on the sliding plate, the first connecting rod (82) and the second connecting rod (83) are hinged and fixed through a rotating shaft of a roller (84), a liquid pocket box (81) is fixedly connected with the sliding plate, the liquid pocket box (81) is arranged below the sliding plate in parallel, and the fixed plate is provided with a sliding rail which is in sliding fit with the sliding plate.

10. The fully automatic sample adding device according to any one of claims 1 to 5, further comprising a waste liquid treatment mechanism (9) for collecting waste liquid, wherein the sample adding needle mechanism (2) can extract the waste liquid in the extraction strip (5) and move the waste liquid to the waste liquid treatment mechanism (9).

11. The fully automatic sample adding device according to claim 10, wherein the waste liquid treatment mechanism (9) comprises a waste liquid box (91) for collecting waste liquid, a float switch (92) for detecting whether the capacity of the waste liquid box (91) reaches a preset capacity, and a liquid suction pipe (93) for evacuating waste liquid in the waste liquid box (91), and the float switch (92) and the liquid suction pipe (93) are both connected to the control device.

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