CN115999195A

CN115999195A - Full-automatic positive pressure solid phase extraction instrument

Info

Publication number: CN115999195A
Application number: CN202310149091.4A
Authority: CN
Inventors: 肖大放
Original assignee: Dayan Biochip Technology Dongguan Co ltd
Current assignee: Dayan Biochip Technology Dongguan Co ltd
Priority date: 2023-02-22
Filing date: 2023-02-22
Publication date: 2023-04-25

Abstract

The invention provides a full-automatic positive pressure solid-phase extraction instrument, which comprises a machine table, a bin mechanism, a liquid transferring mechanism, a discharging cabinet and an extraction system, wherein the bin mechanism comprises a bin body, an upper layer material rack and a lower layer material rack; the top of the machine table is provided with a vertical plate, and the pipetting mechanism comprises an X-axis linear driving device, a Y-axis linear driving device, a Z-axis linear driving device and a pipetting gun; a plurality of first material tanks and second material tanks are arranged on the discharging cabinet; the extraction system comprises a positive pressure generator, a positive pressure cover plate and a negative pressure collecting tank, wherein a base plate is fixedly connected to the vertical plate, the positive pressure cover plate is slidably connected to the bottom of the base plate along the Y-axis direction, positive pressure air holes corresponding to the extraction pillars are distributed on the positive pressure cover plate in a penetrating mode, the positive pressure air holes are communicated with the positive pressure output end of the positive pressure generator, the inside of the negative pressure collecting tank is communicated with the negative pressure output end of the positive pressure generator, automation of the extraction process can be achieved, labor input can be reduced, and convenience is brought to food inspection.

Description

Full-automatic positive pressure solid phase extraction instrument

Technical Field

The invention relates to the field of extractors, in particular to a full-automatic positive pressure solid phase extractor.

Background

Food safety is a very concerned problem, harmful substances in food need to be checked, such as melamine, aflatoxin and the like need to be extracted from the food, the extraction process comprises raw material sampling, column passing and elution, no equipment capable of automatically completing the whole extraction process is available at present, a great deal of manual participation is needed in the extraction process, manpower is wasted, and the execution difficulty of food inspection is increased.

Disclosure of Invention

The invention aims to solve the problems of providing a full-automatic positive pressure solid phase extraction instrument, realizing automation of an extraction process and reducing labor input.

The full-automatic positive pressure solid-phase extraction instrument comprises a machine table, wherein a bin mechanism, a liquid transferring mechanism, a discharging cabinet and an extraction system are arranged on the machine table, the bin mechanism comprises a bin body, an upper layer material rack and a lower layer material rack which are connected in the bin body in a sliding manner along the Y-axis direction, and the upper layer material rack and the lower layer material rack are respectively used for placing extraction small columns and collecting pipes; the top of the machine table is provided with a vertical plate, and the pipetting mechanism comprises an X-axis linear driving device arranged on the vertical plate, a Y-axis linear driving device in driving connection with the X-axis linear driving device, a Z-axis linear driving device in driving connection with the Y-axis linear driving device and a pipetting gun in driving connection with the Z-axis linear driving device; the discharging cabinet is provided with a plurality of first material tanks for placing raw material bottles and a plurality of second material tanks for placing needle frames; the extraction system comprises a positive pressure generator, a positive pressure cover plate and a negative pressure collecting tank, wherein a base plate is fixedly connected to the vertical plate, the positive pressure cover plate is slidably connected to the bottom of the base plate along the Y-axis direction, positive pressure air holes corresponding to the extraction pillars are distributed on the positive pressure cover plate in a penetrating manner, the positive pressure air holes are communicated with the positive pressure output end of the positive pressure generator, and the inside of the negative pressure collecting tank is communicated with the negative pressure output end of the positive pressure generator.

Preferably, the bin body comprises two longitudinal plates oppositely arranged at the top of the machine table, the bin mechanism further comprises an upper drawer and a lower drawer, the upper layer material frame and the lower layer material frame are respectively and fixedly connected in the upper drawer and the lower drawer, and the upper drawer and the lower drawer are slidingly connected between the two longitudinal plates along the Y-axis direction.

Preferably, the upper drawer and the lower drawer are provided with the lug plates on one side, and the lug plates are provided with insertion holes in a penetrating way.

Preferably, the X-axis linear driving device, the Y-axis linear driving device and the Z-axis linear driving device are all screw rod sliding tables.

The beneficial effects of the invention are as follows: the invention provides a full-automatic positive-pressure solid-phase extraction instrument, which is characterized in that an extraction column and a collecting pipe are respectively arranged on an upper layer material frame and a lower layer material frame, the upper layer material frame and the lower layer material frame are pushed to move forwards and backwards to realize the feeding and discharging of the extraction column and the collecting pipe, a raw material bottle filled with food and experiment related reagents is arranged on a first trough, a needle frame is arranged on a second trough, a needle head which can be arranged on the gun head of a liquid-transferring gun is arranged on the needle frame, an X-axis linear driving device, a Y-axis linear driving device and a Z-axis linear driving device can drive the liquid-transferring gun to move in the X-axis direction, the Y-axis linear driving device and the Z-axis linear driving device respectively, so that the liquid-transferring gun can be used for installing the needle head from the needle frame, extracting food or reagents from the raw material bottle and injecting the reagents into the extraction column, when the positive-pressure cover plate is pushed out to enable each positive-pressure air hole to be aligned with the top end of the corresponding extraction column, when the positive-pressure generator works, the positive-pressure air hole pressurizes the top of the extraction column, the negative-pressure collecting tank is vacuumized at the bottom of the extraction column, so that the liquid in the extraction column can flow out, the upper layer material frame is pushed out a distance to be pushed out to the corresponding to the liquid-transferring frame to be aligned with the corresponding collector tube, and the liquid is enabled to drop out from the extraction column, and the raw materials can be conveniently extracted from the extraction column, and materials can drop into the extraction column, and the extraction column can be conveniently detected.

Drawings

Fig. 1 illustrates an outline structure of the present invention.

Fig. 2 illustrates a schematic cross-sectional structure of the present invention.

Fig. 3 illustrates a top view of the present invention.

Fig. 4 illustrates a partially enlarged structural view of the portion a in fig. 1 according to the present invention.

Fig. 5 illustrates a partially enlarged structural view of the portion B of fig. 3 according to the present invention.

Reference numerals illustrate: the machine comprises a machine table 10, a vertical plate 11, a base plate 12, a discharging cabinet 20, a first trough 21, a second trough 22, a bin body 30, a vertical plate 300, an upper layer material rack 31, an upper drawer 310, a lower layer material rack 32, a lower drawer 320, an ear plate 33, an inserting hole 330, an X-axis linear driving device 40, a Y-axis linear driving device 41, a Z-axis linear driving device 42, a liquid gun 43, a positive and negative pressure generator 50, a positive pressure cover plate 51, a positive pressure air hole 510 and a negative pressure collecting tank 52.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present disclosure more apparent, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present disclosure. It will be apparent that the described embodiments are some, but not all, of the embodiments of the present disclosure.

All other embodiments, which can be made by one of ordinary skill in the art without the need for inventive faculty, are intended to be within the scope of the present disclosure, based on the described embodiments of the present disclosure.

Reference is made to fig. 1-5.

The invention provides a full-automatic positive pressure solid-phase extraction instrument, which comprises a machine table 10, wherein the machine table 10 is provided with a bin mechanism, a liquid-moving mechanism, a discharging cabinet 20 and an extraction system, the bin mechanism comprises a bin body 30, an upper layer material rack 31 and a lower layer material rack 32 which are connected in the bin body 30 in a sliding manner along the Y-axis direction, and the upper layer material rack 31 and the lower layer material rack 32 are respectively used for placing extraction small columns and collecting pipes; the top of the machine table 10 is provided with a vertical plate 11, and the pipetting mechanism comprises an X-axis linear driving device 40 arranged on the vertical plate 11, a Y-axis linear driving device 41 in driving connection with the X-axis linear driving device 40, a Z-axis linear driving device 42 in driving connection with the Y-axis linear driving device 41, and a pipetting gun 43 in driving connection with the Z-axis linear driving device 42; the discharging cabinet 20 is provided with a plurality of first material tanks 21 for placing raw material bottles and a plurality of second material tanks 22 for placing needle frames; the extraction system comprises a positive pressure generator 50, a positive pressure cover plate 51 and a negative pressure collecting tank 52, wherein a base plate 12 is fixedly connected to the vertical plate 11, the positive pressure cover plate 51 is slidably connected to the bottom of the base plate 12 along the Y-axis direction, positive pressure air holes 510 which are correspondingly arranged with extraction pillars are arranged on the positive pressure cover plate 51 in a penetrating manner, the positive pressure air holes 510 are communicated with the positive pressure output end of the positive pressure generator 50, and the interior of the negative pressure collecting tank 52 is communicated with the negative pressure output end of the positive pressure generator 50.

The working principle is that, at the beginning, the upper layer material frame 31 and the lower layer material frame 32 extend out of the bin body 30, the extraction small column and the collecting pipe are respectively placed on the upper layer material frame 31 and the lower layer material frame 32, during feeding, the upper layer material frame 31 and the lower layer material frame 32 are pushed into the inner side of the bin body 30, so that the upper layer material frame 31 is positioned above the negative pressure collecting tank 52, the bottom end of the extraction small column is communicated to the inside of the negative pressure collecting tank 52, the lower layer material frame 32 is staggered with the upper layer material frame 31, the lower layer material frame 32 is positioned at the front side of the negative pressure collecting tank 52, raw material bottles containing food and experiment related reagents are placed on the first material tank 21, needle heads are placed on the second material tank 22, and needles which can be mounted on gun heads of the liquid transferring gun 43 are placed on the needle heads. The positive and negative pressure generators 50 can output air pressure and form vacuum, the positive and negative pressure generators 50 push out the positive pressure cover plates 51 to align the positive pressure air holes 510 with the corresponding extraction column top ends, the air cylinder can drive the positive pressure cover plates 51 to move forwards and backwards, the positive and negative pressure generators 50 work, the positive pressure air holes 510 form air pressure and pressurize the extraction column top ends, the negative pressure collecting tank 52 forms vacuum state and vacuumizes the extraction column bottom ends, so that waste liquid in the extraction column flows into the negative pressure collecting tank 52 through the bottom ends, the waste liquid is discharged from the negative pressure collecting tank 52, the upper layer material frame 31 is pushed out a distance to align the extraction columns with the corresponding collecting pipes, the reagent layer of the extraction column 43 is pulled out of the collecting pipe, and the reagent layer can be conveniently pulled out of the extraction column 32.

Based on the above embodiment, the bin body 30 comprises two longitudinal plates 300 oppositely arranged at the top of the machine table 10, the bin mechanism further comprises an upper drawer 310 and a lower drawer 320, the upper layer material rack 31 and the lower layer material rack 32 are respectively and fixedly connected in the upper drawer 310 and the lower drawer 320, the upper drawer 310 and the lower drawer 320 are slidingly connected between the two longitudinal plates 300 along the Y-axis direction, so that the drawer-type push-pull function of the upper drawer 310 and the lower drawer 320 is realized, and the feeding and discharging of extraction pillars and collecting pipes are facilitated.

Based on the above embodiment, the ear plates 33 are disposed on one side of the upper drawer 310 and the lower drawer 320, and the insertion holes 330 are disposed on the ear plates 33. When the upper drawer 310 and the lower drawer 320 are required to be pushed to enter and exit on the bin body 30, the gun head of the pipette 43 can be driven to be inserted into the jack 330, and then the pipette 43 is driven to move along the Y-axis direction, so that the automatic push-pull action of the upper drawer 310 and the lower drawer 320 is realized.

Based on the above embodiment, the X-axis linear driving device 40, the Y-axis linear driving device 41, and the Z-axis linear driving device 42 are all screw rod sliding tables, and have good working stability.

The above embodiments are merely illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solution of the present invention should fall within the scope of protection defined by the claims of the present invention without departing from the spirit of the design of the present invention.

Claims

1. The full-automatic positive pressure solid-phase extraction instrument comprises a machine table, and is characterized in that a bin mechanism, a liquid-moving mechanism, a discharging cabinet and an extraction system are arranged on the machine table, wherein the bin mechanism comprises a bin body, an upper layer material rack and a lower layer material rack which are connected in the bin body in a sliding manner along the Y-axis direction, and the upper layer material rack and the lower layer material rack are respectively used for placing extraction small columns and collecting pipes; the machine table top is provided with a vertical plate, and the pipetting mechanism comprises an X-axis linear driving device arranged on the vertical plate, a Y-axis linear driving device in driving connection with the X-axis linear driving device, a Z-axis linear driving device in driving connection with the Y-axis linear driving device and a pipetting gun in driving connection with the Z-axis linear driving device; the feeding cabinet is provided with a plurality of first material tanks for placing raw material bottles and a plurality of second material tanks for placing needle frames; the extraction system comprises a positive pressure generator, a positive pressure cover plate and a negative pressure collecting tank, wherein a base plate is fixedly connected to the vertical plate, the positive pressure cover plate is slidably connected to the bottom of the base plate along the Y-axis direction, positive pressure air holes corresponding to the extraction small columns are distributed on the positive pressure cover plate in a penetrating mode, the positive pressure air holes are communicated with the positive pressure output end of the positive pressure generator, and the inside of the negative pressure collecting tank is communicated with the negative pressure output end of the positive pressure generator.

2. The full-automatic positive pressure solid-phase extraction instrument according to claim 1, wherein the bin body comprises two longitudinal plates oppositely arranged at the top of the machine table, the bin mechanism further comprises an upper drawer and a lower drawer, the upper layer material rack and the lower layer material rack are respectively and fixedly connected in the upper drawer and the lower drawer, and the upper drawer and the lower drawer are slidably connected between the two longitudinal plates along the Y-axis direction.

3. The full-automatic positive pressure solid phase extraction instrument according to claim 2, wherein ear plates are arranged on one sides of the upper drawer and the lower drawer, and insertion holes are formed in the ear plates in a penetrating mode.

4. The full-automatic positive pressure solid phase extraction instrument according to claim 3, wherein the X-axis linear driving device, the Y-axis linear driving device and the Z-axis linear driving device are screw rod sliding tables.